Efeitos da administração do óleo essencial de pimenta pseudocaryophyllus (Gomes) landrum em modelo animal de câncer colorretal induzido por 1,2 dimetilhidrazina (DMH)

• Autor(a) principal: Souza, Alana Serrano Campelo de
• Data de Publicação: 2017
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório do Centro Universitário Braz Cubas
• Texto Completo: https://repositorio.cruzeirodosul.edu.br/handle/123456789/2261
• topic: Biotecnologia industrial; Neoplasias intestinais - prevenção e controle; Dianthus; CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL

Cancer is a disease characterized by the uncontrolled growth of cells, responsible for the onset of new blood vessels and the invasion of other organs and tissues. Colorectal cancer is an aggressive type of cancer that can cause metastasis. Therefore, it is important to look for alternative ways to prevent and treat this disease. In folk medicine, many teas and extracts of plants are already used empirically in an attempt to prevent cancer development. It is known that the essential oil from Pimenta pseudocaryophyllus (Gomes) Landrum plant is rich in limonene and eugenol, and that these two components have antiproliferative properties. This study aims to (I) review chemopreventive agents for colorectal cancer, especially medicinal herbs and their antitumor properties, (II) review animal models of colorectal carcinogenesis, and its advantages and disadvantages, (III) to evaluate the chemopreventive potential of essential oil extracted form Pimenta pseudocaryophyllus (Gomes) Landrum leafs, against colorectal cancer. This study was approved by ethical committee of animal use, of Universidade Positivo (312/2015). Were used 60 male Wistar rats, witch were divided into five equal groups. Two of the groups received a pretreatment with essential oil of the plant, 5% and 10% concentrate, before subcutaneous administration of tumor inducer, 1,2 dimetilhidrazine (DMH); another group received only the tumor inducer, without pretreatment with essential oil; the fourth group received neither DMH nor the pretreatment with essential oil, but received ethanol orally (used for oil dilution); and finally, the fifth group was defined as been the control group and only received saline 0,9% orally. After 8 months of monitoring, the animals were euthanized in a CO2 chamber. The disease activity indexes were calculated, which ranged from 0, on animals of the control groups, to 3,33, on animal that received the inducer. All animals of DMH-induced groups had intestinal adenocarcinomas in different degrees of differentiation; well differentiated, moderately differentiated, undifferentiated and with gastric differentiation and signet ring cells. The blood collected via cardiac puncture was used to perform immunoenzymatic tests (IL-6), blood count and liver enzymes. A significant difference was observed between animals of control group and animals that received the inducer injections, for all liver enzymes (p<0,01) and for some hematological parameters such as hemoglobin (p<0,01). In addition, concentrations of IL-6 were shown to be significantly higher in animals treated with the essential oil 10% concentrate (p<0,01). The essential oil of P. pseudocaryophyllus was not able to prevent tumor development induced by DMH, and may have potenciated cacinogenic effect promoted by the inducer.