Avaliação da fração vascular estromal da porção anterior do corpo adiposo de Bichat após associação com plasma pobre em plaqueta autólogo como meio de transporte

• Autor(a) principal: Lisboa, Desyree Ghezzi
• Data de Publicação: 2019
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório do Centro Universitário Braz Cubas
• Texto Completo: https://repositorio.cruzeirodosul.edu.br/handle/123456789/2288
• topic: Biotecnologia industrial; Células-tronco; Medicina regenerativa; Tecido adiposo; CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL

Stem cells have several characteristics, such as cellular differentiation and the capacity of adhering to plastic, which allows its cultivation in bottles or cell culture plates. Research is usually conducted utilizing mesenchymal stem cell, due to its multiple applications and for being easily found in adipose tissue. Several procedures extract adipose tissue from the body, which is then usually thrown away. One of them is buccal fat extraction, a commonly practiced plastic surgery procedure, that extracts and disposes of Bichat's fat pad. It contains several different cell types, that together are called stromal vascular fraction (SVF). For a clinical application of the SVF, a maintenance and transportation medium to deliver cells to its designated location can be a fluid and autologous one, such as the platelet poor plasma. This work's objective is to assess the cells that compose the stromal vascular fraction (SVF) and verify their viability after being immersed in platelet poor plasma for up to 48 hours. The Bichat's fat pad of 3 patients submitted to buccal fat extraction were collected and separately submitted to a SVF obtainment protocol. The SVF was divided into parts in syringes and culture plates, at 0, 24 and 48 hour marks. Cellular viability and characterization were evaluated utilizing a flow cytometer. Through immunophenotyping, the following were detected: endothelial cells, T cells, hematopoietic stem cell and mesenchymal stem cells. The Dominici protocol also confirmed the existence of mesenchymal stem cells in the sample. Cellular viability in the platelet poor plasma remained at 70%, a value considered acceptable by the International Society for Cellular Therapy (ISCT). Therefore, while abiding by biosafety measures, the autologous platelet poor plasma can be considered an efficient medium for the clinical application of SVF