Protocol for collection and separation of bone marrow mononuclear cells in Chlorocebus aethiops

Detalhes bibliográficos
Autor(a) principal: Branco,Érika
Data de Publicação: 2016
Outros Autores: Silva,Klena Sarges Marruaz, Assis,Maria de Fátima, Oliveira,Edivaldo Herculano Corrêa de, Lima,Ana Rita de, Aquino,Ana Cleide Guimbal de, Cabral,Rosa, Miglino,Maria Angelica
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Veterinária Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2016000200119
Resumo: Abstract: Chlorocebus aethiops is a species of non-human primate frequently used in biomedical research. Some research involves this species as an experimental model for various diseases and possible treatment with stem cells. The bone marrow is one of the main sources of these cells and provides easy access. The aim of this study was to standardize the protocol of collection and separation of bone marrow in C. aethiops. Ten animals were submitted to puncture of bone marrow with access to the iliac crest and cell separation by density gradient. The bone marrow of C. aethiops had an average of 97% viability. From the results achieved, we can conclude that C. aethiops is an excellent model to obtain and isolate mononuclear cells from bone marrow, fostering several studies in the field of cell therapy.
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spelling Protocol for collection and separation of bone marrow mononuclear cells in Chlorocebus aethiopsBone marrowstem cellmononuclear cellsChlorocebus aethiopsAbstract: Chlorocebus aethiops is a species of non-human primate frequently used in biomedical research. Some research involves this species as an experimental model for various diseases and possible treatment with stem cells. The bone marrow is one of the main sources of these cells and provides easy access. The aim of this study was to standardize the protocol of collection and separation of bone marrow in C. aethiops. Ten animals were submitted to puncture of bone marrow with access to the iliac crest and cell separation by density gradient. The bone marrow of C. aethiops had an average of 97% viability. From the results achieved, we can conclude that C. aethiops is an excellent model to obtain and isolate mononuclear cells from bone marrow, fostering several studies in the field of cell therapy.Colégio Brasileiro de Patologia Animal - CBPA2016-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2016000200119Pesquisa Veterinária Brasileira v.36 n.2 2016reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/S0100-736X2016000200009info:eu-repo/semantics/openAccessBranco,ÉrikaSilva,Klena Sarges MarruazAssis,Maria de FátimaOliveira,Edivaldo Herculano Corrêa deLima,Ana Rita deAquino,Ana Cleide Guimbal deCabral,RosaMiglino,Maria Angelicaeng2016-03-29T00:00:00Zoai:scielo:S0100-736X2016000200119Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2016-03-29T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false
dc.title.none.fl_str_mv Protocol for collection and separation of bone marrow mononuclear cells in Chlorocebus aethiops
title Protocol for collection and separation of bone marrow mononuclear cells in Chlorocebus aethiops
spellingShingle Protocol for collection and separation of bone marrow mononuclear cells in Chlorocebus aethiops
Branco,Érika
Bone marrow
stem cell
mononuclear cells
Chlorocebus aethiops
title_short Protocol for collection and separation of bone marrow mononuclear cells in Chlorocebus aethiops
title_full Protocol for collection and separation of bone marrow mononuclear cells in Chlorocebus aethiops
title_fullStr Protocol for collection and separation of bone marrow mononuclear cells in Chlorocebus aethiops
title_full_unstemmed Protocol for collection and separation of bone marrow mononuclear cells in Chlorocebus aethiops
title_sort Protocol for collection and separation of bone marrow mononuclear cells in Chlorocebus aethiops
author Branco,Érika
author_facet Branco,Érika
Silva,Klena Sarges Marruaz
Assis,Maria de Fátima
Oliveira,Edivaldo Herculano Corrêa de
Lima,Ana Rita de
Aquino,Ana Cleide Guimbal de
Cabral,Rosa
Miglino,Maria Angelica
author_role author
author2 Silva,Klena Sarges Marruaz
Assis,Maria de Fátima
Oliveira,Edivaldo Herculano Corrêa de
Lima,Ana Rita de
Aquino,Ana Cleide Guimbal de
Cabral,Rosa
Miglino,Maria Angelica
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Branco,Érika
Silva,Klena Sarges Marruaz
Assis,Maria de Fátima
Oliveira,Edivaldo Herculano Corrêa de
Lima,Ana Rita de
Aquino,Ana Cleide Guimbal de
Cabral,Rosa
Miglino,Maria Angelica
dc.subject.por.fl_str_mv Bone marrow
stem cell
mononuclear cells
Chlorocebus aethiops
topic Bone marrow
stem cell
mononuclear cells
Chlorocebus aethiops
description Abstract: Chlorocebus aethiops is a species of non-human primate frequently used in biomedical research. Some research involves this species as an experimental model for various diseases and possible treatment with stem cells. The bone marrow is one of the main sources of these cells and provides easy access. The aim of this study was to standardize the protocol of collection and separation of bone marrow in C. aethiops. Ten animals were submitted to puncture of bone marrow with access to the iliac crest and cell separation by density gradient. The bone marrow of C. aethiops had an average of 97% viability. From the results achieved, we can conclude that C. aethiops is an excellent model to obtain and isolate mononuclear cells from bone marrow, fostering several studies in the field of cell therapy.
publishDate 2016
dc.date.none.fl_str_mv 2016-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2016000200119
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2016000200119
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-736X2016000200009
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
dc.source.none.fl_str_mv Pesquisa Veterinária Brasileira v.36 n.2 2016
reponame:Pesquisa Veterinária Brasileira (Online)
instname:Colégio Brasileiro de Patologia Animal (CBPA)
instacron:EMBRAPA
instname_str Colégio Brasileiro de Patologia Animal (CBPA)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Veterinária Brasileira (Online)
collection Pesquisa Veterinária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)
repository.mail.fl_str_mv colegio@cbpa.org.br||pvb@pvb.com.br
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