Protocol for collection and separation of bone marrow mononuclear cells in chlorocebus aethiops

Detalhes bibliográficos
Autor(a) principal: Branco, Érika
Data de Publicação: 2016
Outros Autores: Silva, Klena Sarges Marruaz, Assis, Maria de Fátima, Oliveira, Edivaldo Herculano Corrêa de, Lima, Ana Rita de, Aquino, Ana Cleide Guimbal de, Miglino, Rosa Cabral Maria Angelica
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/36847
Resumo: Chlorocebus aethiops is a species of non-human primate frequently used in biomedical research. Some research involves this species as an experimental model for various diseases and possible treatment with stem cells. The bone marrow is one of the main sources of these cells and provides easy access. The aim of this study was to standardize the protocol of collection and separation of bone marrow in C. aethiops. Ten animals were submitted to puncture of bone marrow with access to the iliac crest and cell separation by density gradient. The bone marrow of C. aethiops had an average of 97% viability. From the results achieved, we can conclude that C. aethiops is an excellent model to obtain and isolate mononuclear cells from bone marrow, fostering several studies in the field of cell therapy.
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spelling Protocol for collection and separation of bone marrow mononuclear cells in chlorocebus aethiopsProtocolo para colheita e isolamento de células mononucleares de medula óssea em Chlorocebus aethiopsBone marrowStem cellMononuclear cellsMedula ósseaCélulas-troncoCélulas mononuclearesChlorocebus aethiops is a species of non-human primate frequently used in biomedical research. Some research involves this species as an experimental model for various diseases and possible treatment with stem cells. The bone marrow is one of the main sources of these cells and provides easy access. The aim of this study was to standardize the protocol of collection and separation of bone marrow in C. aethiops. Ten animals were submitted to puncture of bone marrow with access to the iliac crest and cell separation by density gradient. The bone marrow of C. aethiops had an average of 97% viability. From the results achieved, we can conclude that C. aethiops is an excellent model to obtain and isolate mononuclear cells from bone marrow, fostering several studies in the field of cell therapy.Chlorocebus aethiops é uma espécie de primata não humano frequentemente utilizados em pesquisa biomédica. Algumas pesquisas envolve esta espécie como modelo experimental para várias doenças e possível tratamento com células-tronco. A medula óssea é uma das principais fontes destas células e proporciona fácil acesso. O objetivo deste estudo foi o de padronizar o protocolo de coleta e separação de medula óssea em C. aethiops. Dez animais foram submetidos a punção de medula óssea com acesso à crista ilíaca e separação de células por gradiente de densidade. A medula óssea de C. aethiops tinha uma média de 97% de viabilidade. A partir dos resultados obtidos, podemos concluir que C. aethiops é um excelente modelo para obter e isolar células mononucleares da medula óssea, promovendo vários estudos no campo da terapia celular.Colégio Brasileiro de Patologia Animal (CBPA)2019-09-18T13:50:21Z2019-09-18T13:50:21Z2016-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfBRANCO, É. et al. Protocol for collection and separation of bone marrow mononuclear cells in chlorocebus aethiops. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 2, p. 119-122, Feb. 2016.http://repositorio.ufla.br/jspui/handle/1/36847Pesquisa Veterinária Brasileirareponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessBranco, ÉrikaSilva, Klena Sarges MarruazAssis, Maria de FátimaOliveira, Edivaldo Herculano Corrêa deLima, Ana Rita deAquino, Ana Cleide Guimbal deMiglino, Rosa Cabral Maria Angelicaeng2019-09-18T13:51:10Zoai:localhost:1/36847Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2019-09-18T13:51:10Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Protocol for collection and separation of bone marrow mononuclear cells in chlorocebus aethiops
Protocolo para colheita e isolamento de células mononucleares de medula óssea em Chlorocebus aethiops
title Protocol for collection and separation of bone marrow mononuclear cells in chlorocebus aethiops
spellingShingle Protocol for collection and separation of bone marrow mononuclear cells in chlorocebus aethiops
Branco, Érika
Bone marrow
Stem cell
Mononuclear cells
Medula óssea
Células-tronco
Células mononucleares
title_short Protocol for collection and separation of bone marrow mononuclear cells in chlorocebus aethiops
title_full Protocol for collection and separation of bone marrow mononuclear cells in chlorocebus aethiops
title_fullStr Protocol for collection and separation of bone marrow mononuclear cells in chlorocebus aethiops
title_full_unstemmed Protocol for collection and separation of bone marrow mononuclear cells in chlorocebus aethiops
title_sort Protocol for collection and separation of bone marrow mononuclear cells in chlorocebus aethiops
author Branco, Érika
author_facet Branco, Érika
Silva, Klena Sarges Marruaz
Assis, Maria de Fátima
Oliveira, Edivaldo Herculano Corrêa de
Lima, Ana Rita de
Aquino, Ana Cleide Guimbal de
Miglino, Rosa Cabral Maria Angelica
author_role author
author2 Silva, Klena Sarges Marruaz
Assis, Maria de Fátima
Oliveira, Edivaldo Herculano Corrêa de
Lima, Ana Rita de
Aquino, Ana Cleide Guimbal de
Miglino, Rosa Cabral Maria Angelica
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Branco, Érika
Silva, Klena Sarges Marruaz
Assis, Maria de Fátima
Oliveira, Edivaldo Herculano Corrêa de
Lima, Ana Rita de
Aquino, Ana Cleide Guimbal de
Miglino, Rosa Cabral Maria Angelica
dc.subject.por.fl_str_mv Bone marrow
Stem cell
Mononuclear cells
Medula óssea
Células-tronco
Células mononucleares
topic Bone marrow
Stem cell
Mononuclear cells
Medula óssea
Células-tronco
Células mononucleares
description Chlorocebus aethiops is a species of non-human primate frequently used in biomedical research. Some research involves this species as an experimental model for various diseases and possible treatment with stem cells. The bone marrow is one of the main sources of these cells and provides easy access. The aim of this study was to standardize the protocol of collection and separation of bone marrow in C. aethiops. Ten animals were submitted to puncture of bone marrow with access to the iliac crest and cell separation by density gradient. The bone marrow of C. aethiops had an average of 97% viability. From the results achieved, we can conclude that C. aethiops is an excellent model to obtain and isolate mononuclear cells from bone marrow, fostering several studies in the field of cell therapy.
publishDate 2016
dc.date.none.fl_str_mv 2016-02
2019-09-18T13:50:21Z
2019-09-18T13:50:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv BRANCO, É. et al. Protocol for collection and separation of bone marrow mononuclear cells in chlorocebus aethiops. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 2, p. 119-122, Feb. 2016.
http://repositorio.ufla.br/jspui/handle/1/36847
identifier_str_mv BRANCO, É. et al. Protocol for collection and separation of bone marrow mononuclear cells in chlorocebus aethiops. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 2, p. 119-122, Feb. 2016.
url http://repositorio.ufla.br/jspui/handle/1/36847
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal (CBPA)
publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal (CBPA)
dc.source.none.fl_str_mv Pesquisa Veterinária Brasileira
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
_version_ 1815439230529175552

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