Increased expression of Interleukin-6 related to nephritis in chickens challenged with an Avian infectious bronchitis virus variant

Detalhes bibliográficos
Autor(a) principal: Fernando,Filipe S.
Data de Publicação: 2015
Outros Autores: Okino,Cintia H., Silva,Ketherson R., Fernandes,Camila C., Gonçalves,Mariana C.M., Montassier,Maria F. S., Vasconcelos,Rosemeri O., Montassier,Helio J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Veterinária Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2015000300216
Resumo: A Brazilian field isolate (IBV/Brazil/PR05) of avian infectious bronchitis virus (IBV), associated with development of nephritis in chickens, was previously genotyped as IBV variant after S1 gene sequencing. The aim of this study was to evaluate the levels of IL-6 in kidneys and trachea of birds vaccinated and challenged with IBV/Brazil/PR05 strain, correlating these results with scores of microscopic lesions, specific IBV antigen detection and viral load. The up-regulation of IL-6 and the increased levels of viral load on renal and tracheal samples were significantly correlated with scores of microscopic lesions. Reduced levels of viral load were detected in kidneys of birds previously vaccinated and challenged, compared to non-vaccinated challenged group, although markedly microscopic lesions were observed for both groups. The expression of IL-6, present both in the kidney and in the tracheas, was dependent on the load of the virus present in the tissue, and the development of lesions was related with IL-6 present in the tissues. These data suggest that variant IBV/Brazil/PR05 can induce the expression of proinflammatory cytokines in a manner correlated with viral load and increased IL-6 is involved in the tissue with the influx of inflammatory cells and subsequent nephritis. This may contribute with a model to the development of immunosuppressive agents of IL-6 to prevent acute inflammatory processes against infection with IBV and perhaps other coronaviruses, as well as contribute to the understanding of the immunopathogenesis of IBV nephropatogenic strains.
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spelling Increased expression of Interleukin-6 related to nephritis in chickens challenged with an Avian infectious bronchitis virus variantInterleukin-6nephritisinfectious bronchitis virusIBVvariant strainchicken.A Brazilian field isolate (IBV/Brazil/PR05) of avian infectious bronchitis virus (IBV), associated with development of nephritis in chickens, was previously genotyped as IBV variant after S1 gene sequencing. The aim of this study was to evaluate the levels of IL-6 in kidneys and trachea of birds vaccinated and challenged with IBV/Brazil/PR05 strain, correlating these results with scores of microscopic lesions, specific IBV antigen detection and viral load. The up-regulation of IL-6 and the increased levels of viral load on renal and tracheal samples were significantly correlated with scores of microscopic lesions. Reduced levels of viral load were detected in kidneys of birds previously vaccinated and challenged, compared to non-vaccinated challenged group, although markedly microscopic lesions were observed for both groups. The expression of IL-6, present both in the kidney and in the tracheas, was dependent on the load of the virus present in the tissue, and the development of lesions was related with IL-6 present in the tissues. These data suggest that variant IBV/Brazil/PR05 can induce the expression of proinflammatory cytokines in a manner correlated with viral load and increased IL-6 is involved in the tissue with the influx of inflammatory cells and subsequent nephritis. This may contribute with a model to the development of immunosuppressive agents of IL-6 to prevent acute inflammatory processes against infection with IBV and perhaps other coronaviruses, as well as contribute to the understanding of the immunopathogenesis of IBV nephropatogenic strains.Colégio Brasileiro de Patologia Animal - CBPA2015-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2015000300216Pesquisa Veterinária Brasileira v.35 n.3 2015reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/S0100-736X2015000300002info:eu-repo/semantics/openAccessFernando,Filipe S.Okino,Cintia H.Silva,Ketherson R.Fernandes,Camila C.Gonçalves,Mariana C.M.Montassier,Maria F. S.Vasconcelos,Rosemeri O.Montassier,Helio J.eng2015-07-08T00:00:00Zoai:scielo:S0100-736X2015000300216Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2015-07-08T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false
dc.title.none.fl_str_mv Increased expression of Interleukin-6 related to nephritis in chickens challenged with an Avian infectious bronchitis virus variant
title Increased expression of Interleukin-6 related to nephritis in chickens challenged with an Avian infectious bronchitis virus variant
spellingShingle Increased expression of Interleukin-6 related to nephritis in chickens challenged with an Avian infectious bronchitis virus variant
Fernando,Filipe S.
Interleukin-6
nephritis
infectious bronchitis virus
IBV
variant strain
chicken.
title_short Increased expression of Interleukin-6 related to nephritis in chickens challenged with an Avian infectious bronchitis virus variant
title_full Increased expression of Interleukin-6 related to nephritis in chickens challenged with an Avian infectious bronchitis virus variant
title_fullStr Increased expression of Interleukin-6 related to nephritis in chickens challenged with an Avian infectious bronchitis virus variant
title_full_unstemmed Increased expression of Interleukin-6 related to nephritis in chickens challenged with an Avian infectious bronchitis virus variant
title_sort Increased expression of Interleukin-6 related to nephritis in chickens challenged with an Avian infectious bronchitis virus variant
author Fernando,Filipe S.
author_facet Fernando,Filipe S.
Okino,Cintia H.
Silva,Ketherson R.
Fernandes,Camila C.
Gonçalves,Mariana C.M.
Montassier,Maria F. S.
Vasconcelos,Rosemeri O.
Montassier,Helio J.
author_role author
author2 Okino,Cintia H.
Silva,Ketherson R.
Fernandes,Camila C.
Gonçalves,Mariana C.M.
Montassier,Maria F. S.
Vasconcelos,Rosemeri O.
Montassier,Helio J.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fernando,Filipe S.
Okino,Cintia H.
Silva,Ketherson R.
Fernandes,Camila C.
Gonçalves,Mariana C.M.
Montassier,Maria F. S.
Vasconcelos,Rosemeri O.
Montassier,Helio J.
dc.subject.por.fl_str_mv Interleukin-6
nephritis
infectious bronchitis virus
IBV
variant strain
chicken.
topic Interleukin-6
nephritis
infectious bronchitis virus
IBV
variant strain
chicken.
description A Brazilian field isolate (IBV/Brazil/PR05) of avian infectious bronchitis virus (IBV), associated with development of nephritis in chickens, was previously genotyped as IBV variant after S1 gene sequencing. The aim of this study was to evaluate the levels of IL-6 in kidneys and trachea of birds vaccinated and challenged with IBV/Brazil/PR05 strain, correlating these results with scores of microscopic lesions, specific IBV antigen detection and viral load. The up-regulation of IL-6 and the increased levels of viral load on renal and tracheal samples were significantly correlated with scores of microscopic lesions. Reduced levels of viral load were detected in kidneys of birds previously vaccinated and challenged, compared to non-vaccinated challenged group, although markedly microscopic lesions were observed for both groups. The expression of IL-6, present both in the kidney and in the tracheas, was dependent on the load of the virus present in the tissue, and the development of lesions was related with IL-6 present in the tissues. These data suggest that variant IBV/Brazil/PR05 can induce the expression of proinflammatory cytokines in a manner correlated with viral load and increased IL-6 is involved in the tissue with the influx of inflammatory cells and subsequent nephritis. This may contribute with a model to the development of immunosuppressive agents of IL-6 to prevent acute inflammatory processes against infection with IBV and perhaps other coronaviruses, as well as contribute to the understanding of the immunopathogenesis of IBV nephropatogenic strains.
publishDate 2015
dc.date.none.fl_str_mv 2015-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2015000300216
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2015000300216
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-736X2015000300002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
dc.source.none.fl_str_mv Pesquisa Veterinária Brasileira v.35 n.3 2015
reponame:Pesquisa Veterinária Brasileira (Online)
instname:Colégio Brasileiro de Patologia Animal (CBPA)
instacron:EMBRAPA
instname_str Colégio Brasileiro de Patologia Animal (CBPA)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Veterinária Brasileira (Online)
collection Pesquisa Veterinária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)
repository.mail.fl_str_mv colegio@cbpa.org.br||pvb@pvb.com.br
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