Increased expression of Interleukin-6 related to nephritis in chickens challenged with an Avian infectious bronchitis virus variant
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0100-736X2015000300002 http://hdl.handle.net/11449/171940 |
Resumo: | A Brazilian field isolate (IBV/Brazil/PR05) of avian infectious bronchitis virus (IBV), associated with development of nephritis in chickens, was previously genotyped as IBV variant after S1 gene sequencing. The aim of this study was to evaluate the levels of IL-6 in kidneys and trachea of birds vaccinated and challenged with IBV/Brazil/PR05 strain, correlating these results with scores of microscopic lesions, specific IBV antigen detection and viral load. The up-regulation of IL-6 and the increased levels of viral load on renal and tracheal samples were significantly correlated with scores of microscopic lesions. Reduced levels of viral load were detected in kidneys of birds previously vaccinated and challenged, compared to non-vaccinated challenged group, although markedly microscopic lesions were observed for both groups. The expression of IL-6, present both in the kidney and in the tracheas, was dependent on the load of the virus present in the tissue, and the development of lesions was related with IL-6 present in the tissues. These data suggest that variant IBV/Brazil/PR05 can induce the expression of proinflammatory cytokines in a manner correlated with viral load and increased IL-6 is involved in the tissue with the influx of inflammatory cells and subsequent nephritis. This may contribute with a model to the development of immunosuppressive agents of IL-6 to prevent acute inflammatory processes against infection with IBV and perhaps other coronaviruses, as well as contribute to the understanding of the immunopathogenesis of IBV nephropatogenic strains. |
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Increased expression of Interleukin-6 related to nephritis in chickens challenged with an Avian infectious bronchitis virus variantChickenIBVInfectious bronchitis virusInterleukin-6NephritisVariant strainA Brazilian field isolate (IBV/Brazil/PR05) of avian infectious bronchitis virus (IBV), associated with development of nephritis in chickens, was previously genotyped as IBV variant after S1 gene sequencing. The aim of this study was to evaluate the levels of IL-6 in kidneys and trachea of birds vaccinated and challenged with IBV/Brazil/PR05 strain, correlating these results with scores of microscopic lesions, specific IBV antigen detection and viral load. The up-regulation of IL-6 and the increased levels of viral load on renal and tracheal samples were significantly correlated with scores of microscopic lesions. Reduced levels of viral load were detected in kidneys of birds previously vaccinated and challenged, compared to non-vaccinated challenged group, although markedly microscopic lesions were observed for both groups. The expression of IL-6, present both in the kidney and in the tracheas, was dependent on the load of the virus present in the tissue, and the development of lesions was related with IL-6 present in the tissues. These data suggest that variant IBV/Brazil/PR05 can induce the expression of proinflammatory cytokines in a manner correlated with viral load and increased IL-6 is involved in the tissue with the influx of inflammatory cells and subsequent nephritis. This may contribute with a model to the development of immunosuppressive agents of IL-6 to prevent acute inflammatory processes against infection with IBV and perhaps other coronaviruses, as well as contribute to the understanding of the immunopathogenesis of IBV nephropatogenic strains.Laboratorio de Imunologia e Virologia Veterinaria, Departamento de Patologia Veterinaria, Faculdade de Ciências Agrárias e Veterinárias (FCAV), Universidade Estadual Paulista (Unesp), Via de acesso Prof. Paulo Donato Castellane s/nEmbrapa Suínos e Aves, Rodovia BR-153 Km 110Laboratorio de Imunologia e Virologia Veterinaria, Departamento de Patologia Veterinaria, Faculdade de Ciências Agrárias e Veterinárias (FCAV), Universidade Estadual Paulista (Unesp), Via de acesso Prof. Paulo Donato Castellane s/nUniversidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Fernando, Filipe S. [UNESP]Okino, Cintia H. [UNESP]Silva, Ketherson R. [UNESP]Fernandes, Camila C. [UNESP]Gonçalves, Mariana C. M. [UNESP]Montassier, Maria F. S. [UNESP]Vasconcelos, Rosemeri O. [UNESP]Montassier, Helio J. [UNESP]2018-12-11T16:57:48Z2018-12-11T16:57:48Z2015-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article216-222application/pdfhttp://dx.doi.org/10.1590/S0100-736X2015000300002Pesquisa Veterinaria Brasileira, v. 35, n. 3, p. 216-222, 2015.1678-51500100-736Xhttp://hdl.handle.net/11449/17194010.1590/S0100-736X2015000300002S0100-736X20150003002162-s2.0-84937410807S0100-736X2015000300216.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPesquisa Veterinaria Brasileirainfo:eu-repo/semantics/openAccess2024-01-21T06:26:06Zoai:repositorio.unesp.br:11449/171940Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:36:40.362499Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Increased expression of Interleukin-6 related to nephritis in chickens challenged with an Avian infectious bronchitis virus variant |
title |
Increased expression of Interleukin-6 related to nephritis in chickens challenged with an Avian infectious bronchitis virus variant |
spellingShingle |
Increased expression of Interleukin-6 related to nephritis in chickens challenged with an Avian infectious bronchitis virus variant Fernando, Filipe S. [UNESP] Chicken IBV Infectious bronchitis virus Interleukin-6 Nephritis Variant strain |
title_short |
Increased expression of Interleukin-6 related to nephritis in chickens challenged with an Avian infectious bronchitis virus variant |
title_full |
Increased expression of Interleukin-6 related to nephritis in chickens challenged with an Avian infectious bronchitis virus variant |
title_fullStr |
Increased expression of Interleukin-6 related to nephritis in chickens challenged with an Avian infectious bronchitis virus variant |
title_full_unstemmed |
Increased expression of Interleukin-6 related to nephritis in chickens challenged with an Avian infectious bronchitis virus variant |
title_sort |
Increased expression of Interleukin-6 related to nephritis in chickens challenged with an Avian infectious bronchitis virus variant |
author |
Fernando, Filipe S. [UNESP] |
author_facet |
Fernando, Filipe S. [UNESP] Okino, Cintia H. [UNESP] Silva, Ketherson R. [UNESP] Fernandes, Camila C. [UNESP] Gonçalves, Mariana C. M. [UNESP] Montassier, Maria F. S. [UNESP] Vasconcelos, Rosemeri O. [UNESP] Montassier, Helio J. [UNESP] |
author_role |
author |
author2 |
Okino, Cintia H. [UNESP] Silva, Ketherson R. [UNESP] Fernandes, Camila C. [UNESP] Gonçalves, Mariana C. M. [UNESP] Montassier, Maria F. S. [UNESP] Vasconcelos, Rosemeri O. [UNESP] Montassier, Helio J. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
dc.contributor.author.fl_str_mv |
Fernando, Filipe S. [UNESP] Okino, Cintia H. [UNESP] Silva, Ketherson R. [UNESP] Fernandes, Camila C. [UNESP] Gonçalves, Mariana C. M. [UNESP] Montassier, Maria F. S. [UNESP] Vasconcelos, Rosemeri O. [UNESP] Montassier, Helio J. [UNESP] |
dc.subject.por.fl_str_mv |
Chicken IBV Infectious bronchitis virus Interleukin-6 Nephritis Variant strain |
topic |
Chicken IBV Infectious bronchitis virus Interleukin-6 Nephritis Variant strain |
description |
A Brazilian field isolate (IBV/Brazil/PR05) of avian infectious bronchitis virus (IBV), associated with development of nephritis in chickens, was previously genotyped as IBV variant after S1 gene sequencing. The aim of this study was to evaluate the levels of IL-6 in kidneys and trachea of birds vaccinated and challenged with IBV/Brazil/PR05 strain, correlating these results with scores of microscopic lesions, specific IBV antigen detection and viral load. The up-regulation of IL-6 and the increased levels of viral load on renal and tracheal samples were significantly correlated with scores of microscopic lesions. Reduced levels of viral load were detected in kidneys of birds previously vaccinated and challenged, compared to non-vaccinated challenged group, although markedly microscopic lesions were observed for both groups. The expression of IL-6, present both in the kidney and in the tracheas, was dependent on the load of the virus present in the tissue, and the development of lesions was related with IL-6 present in the tissues. These data suggest that variant IBV/Brazil/PR05 can induce the expression of proinflammatory cytokines in a manner correlated with viral load and increased IL-6 is involved in the tissue with the influx of inflammatory cells and subsequent nephritis. This may contribute with a model to the development of immunosuppressive agents of IL-6 to prevent acute inflammatory processes against infection with IBV and perhaps other coronaviruses, as well as contribute to the understanding of the immunopathogenesis of IBV nephropatogenic strains. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-01-01 2018-12-11T16:57:48Z 2018-12-11T16:57:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0100-736X2015000300002 Pesquisa Veterinaria Brasileira, v. 35, n. 3, p. 216-222, 2015. 1678-5150 0100-736X http://hdl.handle.net/11449/171940 10.1590/S0100-736X2015000300002 S0100-736X2015000300216 2-s2.0-84937410807 S0100-736X2015000300216.pdf |
url |
http://dx.doi.org/10.1590/S0100-736X2015000300002 http://hdl.handle.net/11449/171940 |
identifier_str_mv |
Pesquisa Veterinaria Brasileira, v. 35, n. 3, p. 216-222, 2015. 1678-5150 0100-736X 10.1590/S0100-736X2015000300002 S0100-736X2015000300216 2-s2.0-84937410807 S0100-736X2015000300216.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pesquisa Veterinaria Brasileira |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
216-222 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129536218365952 |