Increased expression of Interleukin-6 related to nephritis in chickens challenged with an Avian infectious bronchitis virus variant

Detalhes bibliográficos
Autor(a) principal: Fernando, Filipe S. [UNESP]
Data de Publicação: 2015
Outros Autores: Okino, Cintia H. [UNESP], Silva, Ketherson R. [UNESP], Fernandes, Camila C. [UNESP], Gonçalves, Mariana C. M. [UNESP], Montassier, Maria F. S. [UNESP], Vasconcelos, Rosemeri O. [UNESP], Montassier, Helio J. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0100-736X2015000300002
http://hdl.handle.net/11449/171940
Resumo: A Brazilian field isolate (IBV/Brazil/PR05) of avian infectious bronchitis virus (IBV), associated with development of nephritis in chickens, was previously genotyped as IBV variant after S1 gene sequencing. The aim of this study was to evaluate the levels of IL-6 in kidneys and trachea of birds vaccinated and challenged with IBV/Brazil/PR05 strain, correlating these results with scores of microscopic lesions, specific IBV antigen detection and viral load. The up-regulation of IL-6 and the increased levels of viral load on renal and tracheal samples were significantly correlated with scores of microscopic lesions. Reduced levels of viral load were detected in kidneys of birds previously vaccinated and challenged, compared to non-vaccinated challenged group, although markedly microscopic lesions were observed for both groups. The expression of IL-6, present both in the kidney and in the tracheas, was dependent on the load of the virus present in the tissue, and the development of lesions was related with IL-6 present in the tissues. These data suggest that variant IBV/Brazil/PR05 can induce the expression of proinflammatory cytokines in a manner correlated with viral load and increased IL-6 is involved in the tissue with the influx of inflammatory cells and subsequent nephritis. This may contribute with a model to the development of immunosuppressive agents of IL-6 to prevent acute inflammatory processes against infection with IBV and perhaps other coronaviruses, as well as contribute to the understanding of the immunopathogenesis of IBV nephropatogenic strains.
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spelling Increased expression of Interleukin-6 related to nephritis in chickens challenged with an Avian infectious bronchitis virus variantChickenIBVInfectious bronchitis virusInterleukin-6NephritisVariant strainA Brazilian field isolate (IBV/Brazil/PR05) of avian infectious bronchitis virus (IBV), associated with development of nephritis in chickens, was previously genotyped as IBV variant after S1 gene sequencing. The aim of this study was to evaluate the levels of IL-6 in kidneys and trachea of birds vaccinated and challenged with IBV/Brazil/PR05 strain, correlating these results with scores of microscopic lesions, specific IBV antigen detection and viral load. The up-regulation of IL-6 and the increased levels of viral load on renal and tracheal samples were significantly correlated with scores of microscopic lesions. Reduced levels of viral load were detected in kidneys of birds previously vaccinated and challenged, compared to non-vaccinated challenged group, although markedly microscopic lesions were observed for both groups. The expression of IL-6, present both in the kidney and in the tracheas, was dependent on the load of the virus present in the tissue, and the development of lesions was related with IL-6 present in the tissues. These data suggest that variant IBV/Brazil/PR05 can induce the expression of proinflammatory cytokines in a manner correlated with viral load and increased IL-6 is involved in the tissue with the influx of inflammatory cells and subsequent nephritis. This may contribute with a model to the development of immunosuppressive agents of IL-6 to prevent acute inflammatory processes against infection with IBV and perhaps other coronaviruses, as well as contribute to the understanding of the immunopathogenesis of IBV nephropatogenic strains.Laboratorio de Imunologia e Virologia Veterinaria, Departamento de Patologia Veterinaria, Faculdade de Ciências Agrárias e Veterinárias (FCAV), Universidade Estadual Paulista (Unesp), Via de acesso Prof. Paulo Donato Castellane s/nEmbrapa Suínos e Aves, Rodovia BR-153 Km 110Laboratorio de Imunologia e Virologia Veterinaria, Departamento de Patologia Veterinaria, Faculdade de Ciências Agrárias e Veterinárias (FCAV), Universidade Estadual Paulista (Unesp), Via de acesso Prof. Paulo Donato Castellane s/nUniversidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Fernando, Filipe S. [UNESP]Okino, Cintia H. [UNESP]Silva, Ketherson R. [UNESP]Fernandes, Camila C. [UNESP]Gonçalves, Mariana C. M. [UNESP]Montassier, Maria F. S. [UNESP]Vasconcelos, Rosemeri O. [UNESP]Montassier, Helio J. [UNESP]2018-12-11T16:57:48Z2018-12-11T16:57:48Z2015-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article216-222application/pdfhttp://dx.doi.org/10.1590/S0100-736X2015000300002Pesquisa Veterinaria Brasileira, v. 35, n. 3, p. 216-222, 2015.1678-51500100-736Xhttp://hdl.handle.net/11449/17194010.1590/S0100-736X2015000300002S0100-736X20150003002162-s2.0-84937410807S0100-736X2015000300216.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPesquisa Veterinaria Brasileirainfo:eu-repo/semantics/openAccess2024-01-21T06:26:06Zoai:repositorio.unesp.br:11449/171940Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:36:40.362499Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Increased expression of Interleukin-6 related to nephritis in chickens challenged with an Avian infectious bronchitis virus variant
title Increased expression of Interleukin-6 related to nephritis in chickens challenged with an Avian infectious bronchitis virus variant
spellingShingle Increased expression of Interleukin-6 related to nephritis in chickens challenged with an Avian infectious bronchitis virus variant
Fernando, Filipe S. [UNESP]
Chicken
IBV
Infectious bronchitis virus
Interleukin-6
Nephritis
Variant strain
title_short Increased expression of Interleukin-6 related to nephritis in chickens challenged with an Avian infectious bronchitis virus variant
title_full Increased expression of Interleukin-6 related to nephritis in chickens challenged with an Avian infectious bronchitis virus variant
title_fullStr Increased expression of Interleukin-6 related to nephritis in chickens challenged with an Avian infectious bronchitis virus variant
title_full_unstemmed Increased expression of Interleukin-6 related to nephritis in chickens challenged with an Avian infectious bronchitis virus variant
title_sort Increased expression of Interleukin-6 related to nephritis in chickens challenged with an Avian infectious bronchitis virus variant
author Fernando, Filipe S. [UNESP]
author_facet Fernando, Filipe S. [UNESP]
Okino, Cintia H. [UNESP]
Silva, Ketherson R. [UNESP]
Fernandes, Camila C. [UNESP]
Gonçalves, Mariana C. M. [UNESP]
Montassier, Maria F. S. [UNESP]
Vasconcelos, Rosemeri O. [UNESP]
Montassier, Helio J. [UNESP]
author_role author
author2 Okino, Cintia H. [UNESP]
Silva, Ketherson R. [UNESP]
Fernandes, Camila C. [UNESP]
Gonçalves, Mariana C. M. [UNESP]
Montassier, Maria F. S. [UNESP]
Vasconcelos, Rosemeri O. [UNESP]
Montassier, Helio J. [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.author.fl_str_mv Fernando, Filipe S. [UNESP]
Okino, Cintia H. [UNESP]
Silva, Ketherson R. [UNESP]
Fernandes, Camila C. [UNESP]
Gonçalves, Mariana C. M. [UNESP]
Montassier, Maria F. S. [UNESP]
Vasconcelos, Rosemeri O. [UNESP]
Montassier, Helio J. [UNESP]
dc.subject.por.fl_str_mv Chicken
IBV
Infectious bronchitis virus
Interleukin-6
Nephritis
Variant strain
topic Chicken
IBV
Infectious bronchitis virus
Interleukin-6
Nephritis
Variant strain
description A Brazilian field isolate (IBV/Brazil/PR05) of avian infectious bronchitis virus (IBV), associated with development of nephritis in chickens, was previously genotyped as IBV variant after S1 gene sequencing. The aim of this study was to evaluate the levels of IL-6 in kidneys and trachea of birds vaccinated and challenged with IBV/Brazil/PR05 strain, correlating these results with scores of microscopic lesions, specific IBV antigen detection and viral load. The up-regulation of IL-6 and the increased levels of viral load on renal and tracheal samples were significantly correlated with scores of microscopic lesions. Reduced levels of viral load were detected in kidneys of birds previously vaccinated and challenged, compared to non-vaccinated challenged group, although markedly microscopic lesions were observed for both groups. The expression of IL-6, present both in the kidney and in the tracheas, was dependent on the load of the virus present in the tissue, and the development of lesions was related with IL-6 present in the tissues. These data suggest that variant IBV/Brazil/PR05 can induce the expression of proinflammatory cytokines in a manner correlated with viral load and increased IL-6 is involved in the tissue with the influx of inflammatory cells and subsequent nephritis. This may contribute with a model to the development of immunosuppressive agents of IL-6 to prevent acute inflammatory processes against infection with IBV and perhaps other coronaviruses, as well as contribute to the understanding of the immunopathogenesis of IBV nephropatogenic strains.
publishDate 2015
dc.date.none.fl_str_mv 2015-01-01
2018-12-11T16:57:48Z
2018-12-11T16:57:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0100-736X2015000300002
Pesquisa Veterinaria Brasileira, v. 35, n. 3, p. 216-222, 2015.
1678-5150
0100-736X
http://hdl.handle.net/11449/171940
10.1590/S0100-736X2015000300002
S0100-736X2015000300216
2-s2.0-84937410807
S0100-736X2015000300216.pdf
url http://dx.doi.org/10.1590/S0100-736X2015000300002
http://hdl.handle.net/11449/171940
identifier_str_mv Pesquisa Veterinaria Brasileira, v. 35, n. 3, p. 216-222, 2015.
1678-5150
0100-736X
10.1590/S0100-736X2015000300002
S0100-736X2015000300216
2-s2.0-84937410807
S0100-736X2015000300216.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Pesquisa Veterinaria Brasileira
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 216-222
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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