Histophilus somni-induced thrombotic meningoencephalitis in cattle from northern Paraná, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Veterinária Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2015000400329 |
Resumo: | Thrombotic meningoencephalitis (TME) is a fatal neurological disease of cattle, predominantly from North America, that is caused by Histophilus somniwith sporadic descriptions from other countries. This manuscript describes the occurrence of spontaneous TME in cattle from northern Paraná, Brazil. Most cattle had acute neurological manifestations characteristic of brain dysfunction. Hematological and cerebrospinal fluid analyses were not suggestive of bacterial infections of the brain. Histopathology revealed meningoencephalitis with vasculitis and thrombosis of small vessels that contained discrete neutrophilic and/or lymphocytic infiltrates admixed with fibrin at the brainstem, cerebral cortex, and trigeminal nerve ganglion of all animals. All tissues from the central nervous system used during this study were previously characterized as negative for rabies virus by the direct immunofluorescence assay. PCR and RT-PCR assays investigated the participation of infectious agents associated with bovine neurological disease by targeting specific genes of H. somni, Listeria monocytogenes, bovine herpesvirus -1 and -5, bovine viral diarrhea virus, and ovine herpesvirus-2. PCR and subsequent sequencing resulted in partial fragments of the 16S rRNA gene of H. somni from brain sections of all animals with histopathological diagnosis of TME; all other PCR/RT-PCR assays were negative. These findings confirmed the participation of H. somni in the neuropathological disease observed in these animals, extend the geographical distribution of this disease, and support previous findings of H. somni from Brazil. |
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Histophilus somni-induced thrombotic meningoencephalitis in cattle from northern Paraná, BrazilHistophilus somnithrombotic meningoencephalitisbovine diseaseneuropathologycentral nervous systemmolecular diagnosticshistophilosis.Thrombotic meningoencephalitis (TME) is a fatal neurological disease of cattle, predominantly from North America, that is caused by Histophilus somniwith sporadic descriptions from other countries. This manuscript describes the occurrence of spontaneous TME in cattle from northern Paraná, Brazil. Most cattle had acute neurological manifestations characteristic of brain dysfunction. Hematological and cerebrospinal fluid analyses were not suggestive of bacterial infections of the brain. Histopathology revealed meningoencephalitis with vasculitis and thrombosis of small vessels that contained discrete neutrophilic and/or lymphocytic infiltrates admixed with fibrin at the brainstem, cerebral cortex, and trigeminal nerve ganglion of all animals. All tissues from the central nervous system used during this study were previously characterized as negative for rabies virus by the direct immunofluorescence assay. PCR and RT-PCR assays investigated the participation of infectious agents associated with bovine neurological disease by targeting specific genes of H. somni, Listeria monocytogenes, bovine herpesvirus -1 and -5, bovine viral diarrhea virus, and ovine herpesvirus-2. PCR and subsequent sequencing resulted in partial fragments of the 16S rRNA gene of H. somni from brain sections of all animals with histopathological diagnosis of TME; all other PCR/RT-PCR assays were negative. These findings confirmed the participation of H. somni in the neuropathological disease observed in these animals, extend the geographical distribution of this disease, and support previous findings of H. somni from Brazil.Colégio Brasileiro de Patologia Animal - CBPA2015-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2015000400329Pesquisa Veterinária Brasileira v.35 n.4 2015reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/S0100-736X2015000400003info:eu-repo/semantics/openAccessHeadley,Selwyn A.Bracarense,Ana Paula F.R.L.Oliveira,Victor H.S.Queiroz,Gustavo R.Okano,WernerAlfieri,Alice F.Flaiban,Karina K.M.C.Lisbôa,Júlio A.N.Alfieri,Amauri A.eng2015-09-08T00:00:00Zoai:scielo:S0100-736X2015000400329Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2015-09-08T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false |
dc.title.none.fl_str_mv |
Histophilus somni-induced thrombotic meningoencephalitis in cattle from northern Paraná, Brazil |
title |
Histophilus somni-induced thrombotic meningoencephalitis in cattle from northern Paraná, Brazil |
spellingShingle |
Histophilus somni-induced thrombotic meningoencephalitis in cattle from northern Paraná, Brazil Headley,Selwyn A. Histophilus somni thrombotic meningoencephalitis bovine disease neuropathology central nervous system molecular diagnostics histophilosis. |
title_short |
Histophilus somni-induced thrombotic meningoencephalitis in cattle from northern Paraná, Brazil |
title_full |
Histophilus somni-induced thrombotic meningoencephalitis in cattle from northern Paraná, Brazil |
title_fullStr |
Histophilus somni-induced thrombotic meningoencephalitis in cattle from northern Paraná, Brazil |
title_full_unstemmed |
Histophilus somni-induced thrombotic meningoencephalitis in cattle from northern Paraná, Brazil |
title_sort |
Histophilus somni-induced thrombotic meningoencephalitis in cattle from northern Paraná, Brazil |
author |
Headley,Selwyn A. |
author_facet |
Headley,Selwyn A. Bracarense,Ana Paula F.R.L. Oliveira,Victor H.S. Queiroz,Gustavo R. Okano,Werner Alfieri,Alice F. Flaiban,Karina K.M.C. Lisbôa,Júlio A.N. Alfieri,Amauri A. |
author_role |
author |
author2 |
Bracarense,Ana Paula F.R.L. Oliveira,Victor H.S. Queiroz,Gustavo R. Okano,Werner Alfieri,Alice F. Flaiban,Karina K.M.C. Lisbôa,Júlio A.N. Alfieri,Amauri A. |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Headley,Selwyn A. Bracarense,Ana Paula F.R.L. Oliveira,Victor H.S. Queiroz,Gustavo R. Okano,Werner Alfieri,Alice F. Flaiban,Karina K.M.C. Lisbôa,Júlio A.N. Alfieri,Amauri A. |
dc.subject.por.fl_str_mv |
Histophilus somni thrombotic meningoencephalitis bovine disease neuropathology central nervous system molecular diagnostics histophilosis. |
topic |
Histophilus somni thrombotic meningoencephalitis bovine disease neuropathology central nervous system molecular diagnostics histophilosis. |
description |
Thrombotic meningoencephalitis (TME) is a fatal neurological disease of cattle, predominantly from North America, that is caused by Histophilus somniwith sporadic descriptions from other countries. This manuscript describes the occurrence of spontaneous TME in cattle from northern Paraná, Brazil. Most cattle had acute neurological manifestations characteristic of brain dysfunction. Hematological and cerebrospinal fluid analyses were not suggestive of bacterial infections of the brain. Histopathology revealed meningoencephalitis with vasculitis and thrombosis of small vessels that contained discrete neutrophilic and/or lymphocytic infiltrates admixed with fibrin at the brainstem, cerebral cortex, and trigeminal nerve ganglion of all animals. All tissues from the central nervous system used during this study were previously characterized as negative for rabies virus by the direct immunofluorescence assay. PCR and RT-PCR assays investigated the participation of infectious agents associated with bovine neurological disease by targeting specific genes of H. somni, Listeria monocytogenes, bovine herpesvirus -1 and -5, bovine viral diarrhea virus, and ovine herpesvirus-2. PCR and subsequent sequencing resulted in partial fragments of the 16S rRNA gene of H. somni from brain sections of all animals with histopathological diagnosis of TME; all other PCR/RT-PCR assays were negative. These findings confirmed the participation of H. somni in the neuropathological disease observed in these animals, extend the geographical distribution of this disease, and support previous findings of H. somni from Brazil. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2015000400329 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2015000400329 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-736X2015000400003 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
dc.source.none.fl_str_mv |
Pesquisa Veterinária Brasileira v.35 n.4 2015 reponame:Pesquisa Veterinária Brasileira (Online) instname:Colégio Brasileiro de Patologia Animal (CBPA) instacron:EMBRAPA |
instname_str |
Colégio Brasileiro de Patologia Animal (CBPA) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Veterinária Brasileira (Online) |
collection |
Pesquisa Veterinária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA) |
repository.mail.fl_str_mv |
colegio@cbpa.org.br||pvb@pvb.com.br |
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1754122235128315904 |