Parameter estimation and use of gamma interferon assay for the diagnosis of bovine tuberculosis in Brazil

Detalhes bibliográficos
Autor(a) principal: Lopes,Luciano B.
Data de Publicação: 2012
Outros Autores: Alves,Telma M., Stynen,Ana Paula R., Mota,Pedro M.P.C., Leite,Rômulo C., Lage,Andrey P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Veterinária Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2012000400001
Resumo: This study aimed to evaluate the interference of tuberculin test on the gamma-interferon (INFg) assay, to estimate the sensitivity and specificity of the INFg assay in Brazilian conditions, and to simulate multiple testing using the comparative tuberculin test and the INFg assay. Three hundred-fifty cattle from two TB-free and two TB-infected herds were submitted to the comparative tuberculin test and the INFg assay. The comparative tuberculin test was performed using avian and bovine PPD. The INFg assay was performed by the BovigamTM kit (CSL Veterinary, Australia), according to the manufacturer's specifications. Sensitivity and specificity of the INFg assay were assessed by a Bayesian latent class model. These diagnostic parameters were also estimate for multiple testing. The results of INFg assay on D0 and D3 after the comparative tuberculin test were compared by the McNemar's test and kappa statistics. Results of mean optical density from INFg assay on both days were similar. Sensitivity and specificity of the INFg assay showed results varying (95% confidence intervals) from 72 to 100% and 74 to 100% respectively. Sensitivity of parallel testing was over 97.5%, while specificity of serial testing was over 99.7%. The INFg assay proved to be a very useful diagnostic method.
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spelling Parameter estimation and use of gamma interferon assay for the diagnosis of bovine tuberculosis in BrazilMycobacterium bovisgamma interferoncomparative tuberculin testdiagnosisThis study aimed to evaluate the interference of tuberculin test on the gamma-interferon (INFg) assay, to estimate the sensitivity and specificity of the INFg assay in Brazilian conditions, and to simulate multiple testing using the comparative tuberculin test and the INFg assay. Three hundred-fifty cattle from two TB-free and two TB-infected herds were submitted to the comparative tuberculin test and the INFg assay. The comparative tuberculin test was performed using avian and bovine PPD. The INFg assay was performed by the BovigamTM kit (CSL Veterinary, Australia), according to the manufacturer's specifications. Sensitivity and specificity of the INFg assay were assessed by a Bayesian latent class model. These diagnostic parameters were also estimate for multiple testing. The results of INFg assay on D0 and D3 after the comparative tuberculin test were compared by the McNemar's test and kappa statistics. Results of mean optical density from INFg assay on both days were similar. Sensitivity and specificity of the INFg assay showed results varying (95% confidence intervals) from 72 to 100% and 74 to 100% respectively. Sensitivity of parallel testing was over 97.5%, while specificity of serial testing was over 99.7%. The INFg assay proved to be a very useful diagnostic method.Colégio Brasileiro de Patologia Animal - CBPA2012-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2012000400001Pesquisa Veterinária Brasileira v.32 n.4 2012reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/S0100-736X2012000400001info:eu-repo/semantics/openAccessLopes,Luciano B.Alves,Telma M.Stynen,Ana Paula R.Mota,Pedro M.P.C.Leite,Rômulo C.Lage,Andrey P.eng2012-06-06T00:00:00Zoai:scielo:S0100-736X2012000400001Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2012-06-06T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false
dc.title.none.fl_str_mv Parameter estimation and use of gamma interferon assay for the diagnosis of bovine tuberculosis in Brazil
title Parameter estimation and use of gamma interferon assay for the diagnosis of bovine tuberculosis in Brazil
spellingShingle Parameter estimation and use of gamma interferon assay for the diagnosis of bovine tuberculosis in Brazil
Lopes,Luciano B.
Mycobacterium bovis
gamma interferon
comparative tuberculin test
diagnosis
title_short Parameter estimation and use of gamma interferon assay for the diagnosis of bovine tuberculosis in Brazil
title_full Parameter estimation and use of gamma interferon assay for the diagnosis of bovine tuberculosis in Brazil
title_fullStr Parameter estimation and use of gamma interferon assay for the diagnosis of bovine tuberculosis in Brazil
title_full_unstemmed Parameter estimation and use of gamma interferon assay for the diagnosis of bovine tuberculosis in Brazil
title_sort Parameter estimation and use of gamma interferon assay for the diagnosis of bovine tuberculosis in Brazil
author Lopes,Luciano B.
author_facet Lopes,Luciano B.
Alves,Telma M.
Stynen,Ana Paula R.
Mota,Pedro M.P.C.
Leite,Rômulo C.
Lage,Andrey P.
author_role author
author2 Alves,Telma M.
Stynen,Ana Paula R.
Mota,Pedro M.P.C.
Leite,Rômulo C.
Lage,Andrey P.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Lopes,Luciano B.
Alves,Telma M.
Stynen,Ana Paula R.
Mota,Pedro M.P.C.
Leite,Rômulo C.
Lage,Andrey P.
dc.subject.por.fl_str_mv Mycobacterium bovis
gamma interferon
comparative tuberculin test
diagnosis
topic Mycobacterium bovis
gamma interferon
comparative tuberculin test
diagnosis
description This study aimed to evaluate the interference of tuberculin test on the gamma-interferon (INFg) assay, to estimate the sensitivity and specificity of the INFg assay in Brazilian conditions, and to simulate multiple testing using the comparative tuberculin test and the INFg assay. Three hundred-fifty cattle from two TB-free and two TB-infected herds were submitted to the comparative tuberculin test and the INFg assay. The comparative tuberculin test was performed using avian and bovine PPD. The INFg assay was performed by the BovigamTM kit (CSL Veterinary, Australia), according to the manufacturer's specifications. Sensitivity and specificity of the INFg assay were assessed by a Bayesian latent class model. These diagnostic parameters were also estimate for multiple testing. The results of INFg assay on D0 and D3 after the comparative tuberculin test were compared by the McNemar's test and kappa statistics. Results of mean optical density from INFg assay on both days were similar. Sensitivity and specificity of the INFg assay showed results varying (95% confidence intervals) from 72 to 100% and 74 to 100% respectively. Sensitivity of parallel testing was over 97.5%, while specificity of serial testing was over 99.7%. The INFg assay proved to be a very useful diagnostic method.
publishDate 2012
dc.date.none.fl_str_mv 2012-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2012000400001
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2012000400001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-736X2012000400001
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
dc.source.none.fl_str_mv Pesquisa Veterinária Brasileira v.32 n.4 2012
reponame:Pesquisa Veterinária Brasileira (Online)
instname:Colégio Brasileiro de Patologia Animal (CBPA)
instacron:EMBRAPA
instname_str Colégio Brasileiro de Patologia Animal (CBPA)
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institution EMBRAPA
reponame_str Pesquisa Veterinária Brasileira (Online)
collection Pesquisa Veterinária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)
repository.mail.fl_str_mv colegio@cbpa.org.br||pvb@pvb.com.br
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