Cytometric blood phenotyping in free-living white-eyed parakeet (Psittacara leucophthalmus)
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Veterinária Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2021000100509 |
Resumo: | ABSTRACT: This study aimed at performing cytometric phenotyping of the blood samples from free-living, young white-eyed parakeets (Psittacara leucophthalmus), stained with 3,3-dihexyloxacarbocyanine [DiOC6(3)]. DiOC6(3)-stained whole blood samples from 19 free-living, young white-eyed parakeets were analyzed by flow cytometry and cell types were distinguished by their typical fluorescence in blue laser channel (FL-1) and SSC (side scatter). It was possible to differentiate erythrocytes (58.3±13.6) from leukocytes (32.4±13.1) and some of the leucocyte subpopulations: lymphocytes/thrombocytes (29.7±7.7), monocytes (30.6±8.5), and granulocytes (5.9-26). However, lymphocytes and thrombocytes could not be sorted in the plots. Our study determined that the predominant population in white-eyed parakeet (P. leucophthalmus) was lymphocytes, thrombocytes, and monocytes in the leucocytes gates in comparison to the granulocyte population. The cytometry method and use of DiOC6(3) stain was available for parakeets blood samples and can be studied and applied to other species of parrots. |
id |
EMBRAPA-2_9705b39d4049a33e619c1affba3c12d1 |
---|---|
oai_identifier_str |
oai:scielo:S0100-736X2021000100509 |
network_acronym_str |
EMBRAPA-2 |
network_name_str |
Pesquisa Veterinária Brasileira (Online) |
repository_id_str |
|
spelling |
Cytometric blood phenotyping in free-living white-eyed parakeet (Psittacara leucophthalmus)White-eyed parakeetPsittacara leucophthalmusPsittaciformesavianflow cytometryblood cell phenotypingleukocytesDiOC6(3)wildlife animalsABSTRACT: This study aimed at performing cytometric phenotyping of the blood samples from free-living, young white-eyed parakeets (Psittacara leucophthalmus), stained with 3,3-dihexyloxacarbocyanine [DiOC6(3)]. DiOC6(3)-stained whole blood samples from 19 free-living, young white-eyed parakeets were analyzed by flow cytometry and cell types were distinguished by their typical fluorescence in blue laser channel (FL-1) and SSC (side scatter). It was possible to differentiate erythrocytes (58.3±13.6) from leukocytes (32.4±13.1) and some of the leucocyte subpopulations: lymphocytes/thrombocytes (29.7±7.7), monocytes (30.6±8.5), and granulocytes (5.9-26). However, lymphocytes and thrombocytes could not be sorted in the plots. Our study determined that the predominant population in white-eyed parakeet (P. leucophthalmus) was lymphocytes, thrombocytes, and monocytes in the leucocytes gates in comparison to the granulocyte population. The cytometry method and use of DiOC6(3) stain was available for parakeets blood samples and can be studied and applied to other species of parrots.Colégio Brasileiro de Patologia Animal - CBPA2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2021000100509Pesquisa Veterinária Brasileira v.41 2021reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/1678-5150-pvb-6688info:eu-repo/semantics/openAccessCruz,Nathan R.N.Santi,Mariele deBraz,Larissa Ayane N.Werther,KarinSantana,Áureo E.eng2021-07-02T00:00:00Zoai:scielo:S0100-736X2021000100509Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2021-07-02T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false |
dc.title.none.fl_str_mv |
Cytometric blood phenotyping in free-living white-eyed parakeet (Psittacara leucophthalmus) |
title |
Cytometric blood phenotyping in free-living white-eyed parakeet (Psittacara leucophthalmus) |
spellingShingle |
Cytometric blood phenotyping in free-living white-eyed parakeet (Psittacara leucophthalmus) Cruz,Nathan R.N. White-eyed parakeet Psittacara leucophthalmus Psittaciformes avian flow cytometry blood cell phenotyping leukocytes DiOC6(3) wildlife animals |
title_short |
Cytometric blood phenotyping in free-living white-eyed parakeet (Psittacara leucophthalmus) |
title_full |
Cytometric blood phenotyping in free-living white-eyed parakeet (Psittacara leucophthalmus) |
title_fullStr |
Cytometric blood phenotyping in free-living white-eyed parakeet (Psittacara leucophthalmus) |
title_full_unstemmed |
Cytometric blood phenotyping in free-living white-eyed parakeet (Psittacara leucophthalmus) |
title_sort |
Cytometric blood phenotyping in free-living white-eyed parakeet (Psittacara leucophthalmus) |
author |
Cruz,Nathan R.N. |
author_facet |
Cruz,Nathan R.N. Santi,Mariele de Braz,Larissa Ayane N. Werther,Karin Santana,Áureo E. |
author_role |
author |
author2 |
Santi,Mariele de Braz,Larissa Ayane N. Werther,Karin Santana,Áureo E. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Cruz,Nathan R.N. Santi,Mariele de Braz,Larissa Ayane N. Werther,Karin Santana,Áureo E. |
dc.subject.por.fl_str_mv |
White-eyed parakeet Psittacara leucophthalmus Psittaciformes avian flow cytometry blood cell phenotyping leukocytes DiOC6(3) wildlife animals |
topic |
White-eyed parakeet Psittacara leucophthalmus Psittaciformes avian flow cytometry blood cell phenotyping leukocytes DiOC6(3) wildlife animals |
description |
ABSTRACT: This study aimed at performing cytometric phenotyping of the blood samples from free-living, young white-eyed parakeets (Psittacara leucophthalmus), stained with 3,3-dihexyloxacarbocyanine [DiOC6(3)]. DiOC6(3)-stained whole blood samples from 19 free-living, young white-eyed parakeets were analyzed by flow cytometry and cell types were distinguished by their typical fluorescence in blue laser channel (FL-1) and SSC (side scatter). It was possible to differentiate erythrocytes (58.3±13.6) from leukocytes (32.4±13.1) and some of the leucocyte subpopulations: lymphocytes/thrombocytes (29.7±7.7), monocytes (30.6±8.5), and granulocytes (5.9-26). However, lymphocytes and thrombocytes could not be sorted in the plots. Our study determined that the predominant population in white-eyed parakeet (P. leucophthalmus) was lymphocytes, thrombocytes, and monocytes in the leucocytes gates in comparison to the granulocyte population. The cytometry method and use of DiOC6(3) stain was available for parakeets blood samples and can be studied and applied to other species of parrots. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2021000100509 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2021000100509 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-5150-pvb-6688 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
dc.source.none.fl_str_mv |
Pesquisa Veterinária Brasileira v.41 2021 reponame:Pesquisa Veterinária Brasileira (Online) instname:Colégio Brasileiro de Patologia Animal (CBPA) instacron:EMBRAPA |
instname_str |
Colégio Brasileiro de Patologia Animal (CBPA) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Veterinária Brasileira (Online) |
collection |
Pesquisa Veterinária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA) |
repository.mail.fl_str_mv |
colegio@cbpa.org.br||pvb@pvb.com.br |
_version_ |
1754122241237319680 |