Cytometric blood phenotyping in free-living white-eyed parakeet (Psittacara leucophthalmus)

Detalhes bibliográficos
Autor(a) principal: Cruz,Nathan R.N.
Data de Publicação: 2021
Outros Autores: Santi,Mariele de, Braz,Larissa Ayane N., Werther,Karin, Santana,Áureo E.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Veterinária Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2021000100509
Resumo: ABSTRACT: This study aimed at performing cytometric phenotyping of the blood samples from free-living, young white-eyed parakeets (Psittacara leucophthalmus), stained with 3,3-dihexyloxacarbocyanine [DiOC6(3)]. DiOC6(3)-stained whole blood samples from 19 free-living, young white-eyed parakeets were analyzed by flow cytometry and cell types were distinguished by their typical fluorescence in blue laser channel (FL-1) and SSC (side scatter). It was possible to differentiate erythrocytes (58.3±13.6) from leukocytes (32.4±13.1) and some of the leucocyte subpopulations: lymphocytes/thrombocytes (29.7±7.7), monocytes (30.6±8.5), and granulocytes (5.9-26). However, lymphocytes and thrombocytes could not be sorted in the plots. Our study determined that the predominant population in white-eyed parakeet (P. leucophthalmus) was lymphocytes, thrombocytes, and monocytes in the leucocytes gates in comparison to the granulocyte population. The cytometry method and use of DiOC6(3) stain was available for parakeets blood samples and can be studied and applied to other species of parrots.
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spelling Cytometric blood phenotyping in free-living white-eyed parakeet (Psittacara leucophthalmus)White-eyed parakeetPsittacara leucophthalmusPsittaciformesavianflow cytometryblood cell phenotypingleukocytesDiOC6(3)wildlife animalsABSTRACT: This study aimed at performing cytometric phenotyping of the blood samples from free-living, young white-eyed parakeets (Psittacara leucophthalmus), stained with 3,3-dihexyloxacarbocyanine [DiOC6(3)]. DiOC6(3)-stained whole blood samples from 19 free-living, young white-eyed parakeets were analyzed by flow cytometry and cell types were distinguished by their typical fluorescence in blue laser channel (FL-1) and SSC (side scatter). It was possible to differentiate erythrocytes (58.3±13.6) from leukocytes (32.4±13.1) and some of the leucocyte subpopulations: lymphocytes/thrombocytes (29.7±7.7), monocytes (30.6±8.5), and granulocytes (5.9-26). However, lymphocytes and thrombocytes could not be sorted in the plots. Our study determined that the predominant population in white-eyed parakeet (P. leucophthalmus) was lymphocytes, thrombocytes, and monocytes in the leucocytes gates in comparison to the granulocyte population. The cytometry method and use of DiOC6(3) stain was available for parakeets blood samples and can be studied and applied to other species of parrots.Colégio Brasileiro de Patologia Animal - CBPA2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2021000100509Pesquisa Veterinária Brasileira v.41 2021reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/1678-5150-pvb-6688info:eu-repo/semantics/openAccessCruz,Nathan R.N.Santi,Mariele deBraz,Larissa Ayane N.Werther,KarinSantana,Áureo E.eng2021-07-02T00:00:00Zoai:scielo:S0100-736X2021000100509Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2021-07-02T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false
dc.title.none.fl_str_mv Cytometric blood phenotyping in free-living white-eyed parakeet (Psittacara leucophthalmus)
title Cytometric blood phenotyping in free-living white-eyed parakeet (Psittacara leucophthalmus)
spellingShingle Cytometric blood phenotyping in free-living white-eyed parakeet (Psittacara leucophthalmus)
Cruz,Nathan R.N.
White-eyed parakeet
Psittacara leucophthalmus
Psittaciformes
avian
flow cytometry
blood cell phenotyping
leukocytes
DiOC6(3)
wildlife animals
title_short Cytometric blood phenotyping in free-living white-eyed parakeet (Psittacara leucophthalmus)
title_full Cytometric blood phenotyping in free-living white-eyed parakeet (Psittacara leucophthalmus)
title_fullStr Cytometric blood phenotyping in free-living white-eyed parakeet (Psittacara leucophthalmus)
title_full_unstemmed Cytometric blood phenotyping in free-living white-eyed parakeet (Psittacara leucophthalmus)
title_sort Cytometric blood phenotyping in free-living white-eyed parakeet (Psittacara leucophthalmus)
author Cruz,Nathan R.N.
author_facet Cruz,Nathan R.N.
Santi,Mariele de
Braz,Larissa Ayane N.
Werther,Karin
Santana,Áureo E.
author_role author
author2 Santi,Mariele de
Braz,Larissa Ayane N.
Werther,Karin
Santana,Áureo E.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Cruz,Nathan R.N.
Santi,Mariele de
Braz,Larissa Ayane N.
Werther,Karin
Santana,Áureo E.
dc.subject.por.fl_str_mv White-eyed parakeet
Psittacara leucophthalmus
Psittaciformes
avian
flow cytometry
blood cell phenotyping
leukocytes
DiOC6(3)
wildlife animals
topic White-eyed parakeet
Psittacara leucophthalmus
Psittaciformes
avian
flow cytometry
blood cell phenotyping
leukocytes
DiOC6(3)
wildlife animals
description ABSTRACT: This study aimed at performing cytometric phenotyping of the blood samples from free-living, young white-eyed parakeets (Psittacara leucophthalmus), stained with 3,3-dihexyloxacarbocyanine [DiOC6(3)]. DiOC6(3)-stained whole blood samples from 19 free-living, young white-eyed parakeets were analyzed by flow cytometry and cell types were distinguished by their typical fluorescence in blue laser channel (FL-1) and SSC (side scatter). It was possible to differentiate erythrocytes (58.3±13.6) from leukocytes (32.4±13.1) and some of the leucocyte subpopulations: lymphocytes/thrombocytes (29.7±7.7), monocytes (30.6±8.5), and granulocytes (5.9-26). However, lymphocytes and thrombocytes could not be sorted in the plots. Our study determined that the predominant population in white-eyed parakeet (P. leucophthalmus) was lymphocytes, thrombocytes, and monocytes in the leucocytes gates in comparison to the granulocyte population. The cytometry method and use of DiOC6(3) stain was available for parakeets blood samples and can be studied and applied to other species of parrots.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2021000100509
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2021000100509
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-5150-pvb-6688
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
dc.source.none.fl_str_mv Pesquisa Veterinária Brasileira v.41 2021
reponame:Pesquisa Veterinária Brasileira (Online)
instname:Colégio Brasileiro de Patologia Animal (CBPA)
instacron:EMBRAPA
instname_str Colégio Brasileiro de Patologia Animal (CBPA)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Veterinária Brasileira (Online)
collection Pesquisa Veterinária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)
repository.mail.fl_str_mv colegio@cbpa.org.br||pvb@pvb.com.br
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