Serological monitoring of antibodies for an early diagnosis ofaspergillosis in captive penguins

Detalhes bibliográficos
Autor(a) principal: Cabana,Ângela L.
Data de Publicação: 2015
Outros Autores: Xavier,Melissa O., Poester,Vanice, Klafke,Gabriel B., B.Filho,Pedro L., Martins,Aryse, S.Filho,Rodolfo P., Meireles,Mário C.A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Veterinária Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2015000600573
Resumo: Abstract: This study aimed to evaluate the efficacy of detection of anti-Aspergillus fumigatus antibodies in captive penguins by double radial agar gel immunodiffusion (AGID) for the aspergillosis diagnosis. We included 134 Magellanic penguins (Spheniscus magellanicus) in rehabilitation at the Center for Recovery of Marine Animals (CRAM / FURG). All of them were monitored by AGID weekly until its final destination (death or release), totalizing 660 serum samples studied. All animals were clinically accompanied and post-mortem examinations was performed in penguins that died during the studied period. A total of 28% (37/134) of the penguins died, 89.2% (33/37) due to aspergillosis, 11% (4/37) by other causes and 97 were released. From the 33 animals with proven aspergillosis, 21 presented anti- A. fumigatus antibodies by AGID, being the average interval between death and positive AGID 16.4 days. Twelve animals with negative serology died of aspergillosis. The sensitivity and specificity rates were 63.6% and 95% respectively, and the positive and negative predictive values were 80.7% and 88.9% respectively. These data demonstrate that the serological monitoring for detection of antibodies by AGID can be an important tool for the diagnosis of aspergillosis in penguins.
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spelling Serological monitoring of antibodies for an early diagnosis ofaspergillosis in captive penguinsSpheniscus magellanicusSphenisciformescaptivityAspergillus sppaspergillosisantibodyimmunodiffusionAbstract: This study aimed to evaluate the efficacy of detection of anti-Aspergillus fumigatus antibodies in captive penguins by double radial agar gel immunodiffusion (AGID) for the aspergillosis diagnosis. We included 134 Magellanic penguins (Spheniscus magellanicus) in rehabilitation at the Center for Recovery of Marine Animals (CRAM / FURG). All of them were monitored by AGID weekly until its final destination (death or release), totalizing 660 serum samples studied. All animals were clinically accompanied and post-mortem examinations was performed in penguins that died during the studied period. A total of 28% (37/134) of the penguins died, 89.2% (33/37) due to aspergillosis, 11% (4/37) by other causes and 97 were released. From the 33 animals with proven aspergillosis, 21 presented anti- A. fumigatus antibodies by AGID, being the average interval between death and positive AGID 16.4 days. Twelve animals with negative serology died of aspergillosis. The sensitivity and specificity rates were 63.6% and 95% respectively, and the positive and negative predictive values were 80.7% and 88.9% respectively. These data demonstrate that the serological monitoring for detection of antibodies by AGID can be an important tool for the diagnosis of aspergillosis in penguins.Colégio Brasileiro de Patologia Animal - CBPA2015-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2015000600573Pesquisa Veterinária Brasileira v.35 n.6 2015reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/S0100-736X2015000600015info:eu-repo/semantics/openAccessCabana,Ângela L.Xavier,Melissa O.Poester,VaniceKlafke,Gabriel B.B.Filho,Pedro L.Martins,AryseS.Filho,Rodolfo P.Meireles,Mário C.A.eng2015-11-17T00:00:00Zoai:scielo:S0100-736X2015000600573Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2015-11-17T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false
dc.title.none.fl_str_mv Serological monitoring of antibodies for an early diagnosis ofaspergillosis in captive penguins
title Serological monitoring of antibodies for an early diagnosis ofaspergillosis in captive penguins
spellingShingle Serological monitoring of antibodies for an early diagnosis ofaspergillosis in captive penguins
Cabana,Ângela L.
Spheniscus magellanicus
Sphenisciformes
captivity
Aspergillus spp
aspergillosis
antibody
immunodiffusion
title_short Serological monitoring of antibodies for an early diagnosis ofaspergillosis in captive penguins
title_full Serological monitoring of antibodies for an early diagnosis ofaspergillosis in captive penguins
title_fullStr Serological monitoring of antibodies for an early diagnosis ofaspergillosis in captive penguins
title_full_unstemmed Serological monitoring of antibodies for an early diagnosis ofaspergillosis in captive penguins
title_sort Serological monitoring of antibodies for an early diagnosis ofaspergillosis in captive penguins
author Cabana,Ângela L.
author_facet Cabana,Ângela L.
Xavier,Melissa O.
Poester,Vanice
Klafke,Gabriel B.
B.Filho,Pedro L.
Martins,Aryse
S.Filho,Rodolfo P.
Meireles,Mário C.A.
author_role author
author2 Xavier,Melissa O.
Poester,Vanice
Klafke,Gabriel B.
B.Filho,Pedro L.
Martins,Aryse
S.Filho,Rodolfo P.
Meireles,Mário C.A.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Cabana,Ângela L.
Xavier,Melissa O.
Poester,Vanice
Klafke,Gabriel B.
B.Filho,Pedro L.
Martins,Aryse
S.Filho,Rodolfo P.
Meireles,Mário C.A.
dc.subject.por.fl_str_mv Spheniscus magellanicus
Sphenisciformes
captivity
Aspergillus spp
aspergillosis
antibody
immunodiffusion
topic Spheniscus magellanicus
Sphenisciformes
captivity
Aspergillus spp
aspergillosis
antibody
immunodiffusion
description Abstract: This study aimed to evaluate the efficacy of detection of anti-Aspergillus fumigatus antibodies in captive penguins by double radial agar gel immunodiffusion (AGID) for the aspergillosis diagnosis. We included 134 Magellanic penguins (Spheniscus magellanicus) in rehabilitation at the Center for Recovery of Marine Animals (CRAM / FURG). All of them were monitored by AGID weekly until its final destination (death or release), totalizing 660 serum samples studied. All animals were clinically accompanied and post-mortem examinations was performed in penguins that died during the studied period. A total of 28% (37/134) of the penguins died, 89.2% (33/37) due to aspergillosis, 11% (4/37) by other causes and 97 were released. From the 33 animals with proven aspergillosis, 21 presented anti- A. fumigatus antibodies by AGID, being the average interval between death and positive AGID 16.4 days. Twelve animals with negative serology died of aspergillosis. The sensitivity and specificity rates were 63.6% and 95% respectively, and the positive and negative predictive values were 80.7% and 88.9% respectively. These data demonstrate that the serological monitoring for detection of antibodies by AGID can be an important tool for the diagnosis of aspergillosis in penguins.
publishDate 2015
dc.date.none.fl_str_mv 2015-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2015000600573
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2015000600573
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-736X2015000600015
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
dc.source.none.fl_str_mv Pesquisa Veterinária Brasileira v.35 n.6 2015
reponame:Pesquisa Veterinária Brasileira (Online)
instname:Colégio Brasileiro de Patologia Animal (CBPA)
instacron:EMBRAPA
instname_str Colégio Brasileiro de Patologia Animal (CBPA)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Veterinária Brasileira (Online)
collection Pesquisa Veterinária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)
repository.mail.fl_str_mv colegio@cbpa.org.br||pvb@pvb.com.br
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