Serological monitoring of antibodies for an early diagnosis ofaspergillosis in captive penguins
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Veterinária Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2015000600573 |
Resumo: | Abstract: This study aimed to evaluate the efficacy of detection of anti-Aspergillus fumigatus antibodies in captive penguins by double radial agar gel immunodiffusion (AGID) for the aspergillosis diagnosis. We included 134 Magellanic penguins (Spheniscus magellanicus) in rehabilitation at the Center for Recovery of Marine Animals (CRAM / FURG). All of them were monitored by AGID weekly until its final destination (death or release), totalizing 660 serum samples studied. All animals were clinically accompanied and post-mortem examinations was performed in penguins that died during the studied period. A total of 28% (37/134) of the penguins died, 89.2% (33/37) due to aspergillosis, 11% (4/37) by other causes and 97 were released. From the 33 animals with proven aspergillosis, 21 presented anti- A. fumigatus antibodies by AGID, being the average interval between death and positive AGID 16.4 days. Twelve animals with negative serology died of aspergillosis. The sensitivity and specificity rates were 63.6% and 95% respectively, and the positive and negative predictive values were 80.7% and 88.9% respectively. These data demonstrate that the serological monitoring for detection of antibodies by AGID can be an important tool for the diagnosis of aspergillosis in penguins. |
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Serological monitoring of antibodies for an early diagnosis ofaspergillosis in captive penguinsSpheniscus magellanicusSphenisciformescaptivityAspergillus sppaspergillosisantibodyimmunodiffusionAbstract: This study aimed to evaluate the efficacy of detection of anti-Aspergillus fumigatus antibodies in captive penguins by double radial agar gel immunodiffusion (AGID) for the aspergillosis diagnosis. We included 134 Magellanic penguins (Spheniscus magellanicus) in rehabilitation at the Center for Recovery of Marine Animals (CRAM / FURG). All of them were monitored by AGID weekly until its final destination (death or release), totalizing 660 serum samples studied. All animals were clinically accompanied and post-mortem examinations was performed in penguins that died during the studied period. A total of 28% (37/134) of the penguins died, 89.2% (33/37) due to aspergillosis, 11% (4/37) by other causes and 97 were released. From the 33 animals with proven aspergillosis, 21 presented anti- A. fumigatus antibodies by AGID, being the average interval between death and positive AGID 16.4 days. Twelve animals with negative serology died of aspergillosis. The sensitivity and specificity rates were 63.6% and 95% respectively, and the positive and negative predictive values were 80.7% and 88.9% respectively. These data demonstrate that the serological monitoring for detection of antibodies by AGID can be an important tool for the diagnosis of aspergillosis in penguins.Colégio Brasileiro de Patologia Animal - CBPA2015-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2015000600573Pesquisa Veterinária Brasileira v.35 n.6 2015reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/S0100-736X2015000600015info:eu-repo/semantics/openAccessCabana,Ângela L.Xavier,Melissa O.Poester,VaniceKlafke,Gabriel B.B.Filho,Pedro L.Martins,AryseS.Filho,Rodolfo P.Meireles,Mário C.A.eng2015-11-17T00:00:00Zoai:scielo:S0100-736X2015000600573Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2015-11-17T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false |
dc.title.none.fl_str_mv |
Serological monitoring of antibodies for an early diagnosis ofaspergillosis in captive penguins |
title |
Serological monitoring of antibodies for an early diagnosis ofaspergillosis in captive penguins |
spellingShingle |
Serological monitoring of antibodies for an early diagnosis ofaspergillosis in captive penguins Cabana,Ângela L. Spheniscus magellanicus Sphenisciformes captivity Aspergillus spp aspergillosis antibody immunodiffusion |
title_short |
Serological monitoring of antibodies for an early diagnosis ofaspergillosis in captive penguins |
title_full |
Serological monitoring of antibodies for an early diagnosis ofaspergillosis in captive penguins |
title_fullStr |
Serological monitoring of antibodies for an early diagnosis ofaspergillosis in captive penguins |
title_full_unstemmed |
Serological monitoring of antibodies for an early diagnosis ofaspergillosis in captive penguins |
title_sort |
Serological monitoring of antibodies for an early diagnosis ofaspergillosis in captive penguins |
author |
Cabana,Ângela L. |
author_facet |
Cabana,Ângela L. Xavier,Melissa O. Poester,Vanice Klafke,Gabriel B. B.Filho,Pedro L. Martins,Aryse S.Filho,Rodolfo P. Meireles,Mário C.A. |
author_role |
author |
author2 |
Xavier,Melissa O. Poester,Vanice Klafke,Gabriel B. B.Filho,Pedro L. Martins,Aryse S.Filho,Rodolfo P. Meireles,Mário C.A. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Cabana,Ângela L. Xavier,Melissa O. Poester,Vanice Klafke,Gabriel B. B.Filho,Pedro L. Martins,Aryse S.Filho,Rodolfo P. Meireles,Mário C.A. |
dc.subject.por.fl_str_mv |
Spheniscus magellanicus Sphenisciformes captivity Aspergillus spp aspergillosis antibody immunodiffusion |
topic |
Spheniscus magellanicus Sphenisciformes captivity Aspergillus spp aspergillosis antibody immunodiffusion |
description |
Abstract: This study aimed to evaluate the efficacy of detection of anti-Aspergillus fumigatus antibodies in captive penguins by double radial agar gel immunodiffusion (AGID) for the aspergillosis diagnosis. We included 134 Magellanic penguins (Spheniscus magellanicus) in rehabilitation at the Center for Recovery of Marine Animals (CRAM / FURG). All of them were monitored by AGID weekly until its final destination (death or release), totalizing 660 serum samples studied. All animals were clinically accompanied and post-mortem examinations was performed in penguins that died during the studied period. A total of 28% (37/134) of the penguins died, 89.2% (33/37) due to aspergillosis, 11% (4/37) by other causes and 97 were released. From the 33 animals with proven aspergillosis, 21 presented anti- A. fumigatus antibodies by AGID, being the average interval between death and positive AGID 16.4 days. Twelve animals with negative serology died of aspergillosis. The sensitivity and specificity rates were 63.6% and 95% respectively, and the positive and negative predictive values were 80.7% and 88.9% respectively. These data demonstrate that the serological monitoring for detection of antibodies by AGID can be an important tool for the diagnosis of aspergillosis in penguins. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2015000600573 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2015000600573 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-736X2015000600015 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
dc.source.none.fl_str_mv |
Pesquisa Veterinária Brasileira v.35 n.6 2015 reponame:Pesquisa Veterinária Brasileira (Online) instname:Colégio Brasileiro de Patologia Animal (CBPA) instacron:EMBRAPA |
instname_str |
Colégio Brasileiro de Patologia Animal (CBPA) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Veterinária Brasileira (Online) |
collection |
Pesquisa Veterinária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA) |
repository.mail.fl_str_mv |
colegio@cbpa.org.br||pvb@pvb.com.br |
_version_ |
1754122235472248832 |