Monitoramento sorológico para diagnóstico precoce da aspergilose em pinguins em cativeiro
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFPel - Guaiaca |
Texto Completo: | http://repositorio.ufpel.edu.br/handle/ri/2490 |
Resumo: | Aspergillosis is a leading cause of death in captive penguins. Though being a known problem for decades, most reported cases are still only confirmed in post-mortem examinations, requiring further studies on methods for in vivo diagnosis of the disease. This study aimed to evaluate the efficacy of detection of anti-Aspergillus fumigatus antibodies through the performance by serological monitoring with agar gel double radial immunodiffusion (AGID) for in vivo diagnosis of aspergillosis in captive penguins. The study was performed including Magellanic penguins in a rehabilitation program at Center for Recovery of Marine Animals (CRAM/FURG) in the period from 2009 to 2011. We included 134 Magellanic penguins (Spheniscus magellanicus) in rehabilitation at CRAM, which were monitored by AGID weekly until its final destination (death or release) totaling 660 studied serum samples. The serological monitoring was performed in order to detect anti-Aspergillus fumigatus antibodies using commercial antibodies and antigen. All animals were clinically monitored and post-mortem examinations were performed on penguins that died during the study period. A total of 28% (37/134) penguin died, 89.2% (33/37) of aspergillosis, 11% (4/37) of other causes and 97 were released. From the 33 animals with proven aspergillosis, 21 had anti-A. fumigatus antibodies by AGID, being the average interval between death and the positive AGID result of 16.4 days. Twelve seronegative animals eventually died of aspergillosis. The rates of sensitivity and specificity were 63.6% and 95%, respectively, and positive and negative predictive values were 80.7% and 88.9% respectively. These data demonstrate that serological monitoring for the detection of antibodies by AGID may be a useful and important tool for the diagnosis of aspergillosis in penguins. As our study with AGID in penguins is pioneer, more studies are required concerning the prognosis in cases of aspergillosis in penguins whose therapeutic intervention is based on diagnosis by AGID, considering that the average period between the positive result of this test and the death of the animal was less than one month. Key-words: Sphenisciformes. Captivity |
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2014-08-20T14:37:52Z2013-08-212014-08-20T14:37:52Z2013-02-28CABANA, Ângela Leitzke. Monitoramento sorológico para diagnostic precoce da aspergilose em pinguins em cativeiro. 2013. 110 f. Dissertação (Mestrado em Veterinária) - Universidade Federal de Pelotas, Pelotas, 2013.http://repositorio.ufpel.edu.br/handle/ri/2490Aspergillosis is a leading cause of death in captive penguins. Though being a known problem for decades, most reported cases are still only confirmed in post-mortem examinations, requiring further studies on methods for in vivo diagnosis of the disease. This study aimed to evaluate the efficacy of detection of anti-Aspergillus fumigatus antibodies through the performance by serological monitoring with agar gel double radial immunodiffusion (AGID) for in vivo diagnosis of aspergillosis in captive penguins. The study was performed including Magellanic penguins in a rehabilitation program at Center for Recovery of Marine Animals (CRAM/FURG) in the period from 2009 to 2011. We included 134 Magellanic penguins (Spheniscus magellanicus) in rehabilitation at CRAM, which were monitored by AGID weekly until its final destination (death or release) totaling 660 studied serum samples. The serological monitoring was performed in order to detect anti-Aspergillus fumigatus antibodies using commercial antibodies and antigen. All animals were clinically monitored and post-mortem examinations were performed on penguins that died during the study period. A total of 28% (37/134) penguin died, 89.2% (33/37) of aspergillosis, 11% (4/37) of other causes and 97 were released. From the 33 animals with proven aspergillosis, 21 had anti-A. fumigatus antibodies by AGID, being the average interval between death and the positive AGID result of 16.4 days. Twelve seronegative animals eventually died of aspergillosis. The rates of sensitivity and specificity were 63.6% and 95%, respectively, and positive and negative predictive values were 80.7% and 88.9% respectively. These data demonstrate that serological monitoring for the detection of antibodies by AGID may be a useful and important tool for the diagnosis of aspergillosis in penguins. As our study with AGID in penguins is pioneer, more studies are required concerning the prognosis in cases of aspergillosis in penguins whose therapeutic intervention is based on diagnosis by AGID, considering that the average period between the positive result of this test and the death of the animal was less than one month. Key-words: Sphenisciformes. CaptivityA aspergilose é uma das principais causas de morte de pinguins em cativeiro. Embora este seja um problema conhecido há décadas, a maioria dos casos relatados são ainda confirmados em exames post-mortem, sendo necessários mais estudos sobre os métodos de diagnóstico in vivo da doença. Assim, este trabalho objetivou avaliar a eficácia da detecção de anticorpos anti-Aspergillus fumigatus através do desempenho do monitoramento sorológico por imunodifusão radial dupla em gel de ágar (IDGA) para o diagnóstico in vivo da aspergilose em pinguins em cativeiro. O estudo foi realizado incluindo pinguins-de-Magalhães em reabilitação no Centro de Recuperação de Animais Marinhos (CRAM/FURG) no período de 2009 a 2011.. Foram incluídos 134 pingüins de Magalhães (Spheniscus magellanicus) em reabilitação no Centro de Recuperação de Animais Marinhos (CRAM / FURG), que foram monitorados por IDGA semanalmente até seu destino final (morte ou de liberação), totalizando 660 amostras de soro estudadas. O monitoramento sorológico foi realizado para detecção de anticorpos anti-Aspergillus fumigatus utilizando antígeno e anticorpos comerciais. Todos os animais foram acompanhados clinicamente e exames post-mortem foram realizados em pinguins que vieram a óbito durante o período do estudo. Um total de 28% (37/134) dos pinguins veio a óbito, 89,2% (33/37) de aspergilose, 11% (4/37) de outras causas e 97 foram libertados. A partir dos 33 animais com aspergilose comprovada, 21 apresentaram anticorpos anti- A. fumigatus por IDGA, sendo o intervalo médio entre a morte e as IDGA positivas 16,4 dias. Doze animais com sorologia negativa vieram a óbito por aspergilose. As taxas de sensibilidade e especificidade foram de 63,6% e 95%, respectivamente, e os valores preditivo positivo e negativo foram 80,7% e 88,9%, respectivamente. Estes dados demonstram que o acompanhamento serológico para a detecção de anticorpos por IDGA pode ser uma ferramenta útil e importante para o diagnóstico de aspergilose em pinguins. Como nosso estudo com IDGA em pinguins é pioneiro, maiores estudos são necessários acerca do prognóstico dos casos de aspergilose em pinguins cuja intervenção terapêutica se baseie no diagnóstico por IDGA, considerando que o período médio entre o resultado positivo deste teste e o óbito do animal foi de menos de um mês.application/pdfporUniversidade Federal de PelotasPrograma de Pós-Graduação em VeterináriaUFPelBRVeterináriaVeterináriaSphenisciformesCativeiroAspergillus spAnticorposImunodifusãoIDGAVeterinarySphenisciformesCaptivityAspergillus spAntibodyImmunodiffusionAGIDCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIAMonitoramento sorológico para diagnóstico precoce da aspergilose em pinguins em cativeiroinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttp://lattes.cnpq.br/3380926755008181http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4799478H9Vargas, Gilberto D ávilahttp://lattes.cnpq.br/2905761117990157Meireles, Mario Carlos AraújoCabana, Ângela Leitzkeinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPel - Guaiacainstname:Universidade Federal de Pelotas (UFPEL)instacron:UFPELORIGINALdissertacao_angela_cabana.pdfapplication/pdf1514484http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2490/1/dissertacao_angela_cabana.pdfa7b8213a229cc70d85faecf587014b8aMD51open accessTEXTdissertacao_angela_cabana.pdf.txtdissertacao_angela_cabana.pdf.txtExtracted Texttext/plain206770http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2490/2/dissertacao_angela_cabana.pdf.txtbbda1fcec76a3d9d76c29558f5e1d0beMD52open accessTHUMBNAILdissertacao_angela_cabana.pdf.jpgdissertacao_angela_cabana.pdf.jpgGenerated Thumbnailimage/jpeg1374http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2490/3/dissertacao_angela_cabana.pdf.jpg0a79f3313da32187ad3c04766224f9abMD53open access123456789/24902019-09-17 18:55:36.625open accessoai:guaiaca.ufpel.edu.br:123456789/2490Repositório InstitucionalPUBhttp://repositorio.ufpel.edu.br/oai/requestrippel@ufpel.edu.br || repositorio@ufpel.edu.br || aline.batista@ufpel.edu.bropendoar:2019-09-17T21:55:36Repositório Institucional da UFPel - Guaiaca - Universidade Federal de Pelotas (UFPEL)false |
dc.title.por.fl_str_mv |
Monitoramento sorológico para diagnóstico precoce da aspergilose em pinguins em cativeiro |
title |
Monitoramento sorológico para diagnóstico precoce da aspergilose em pinguins em cativeiro |
spellingShingle |
Monitoramento sorológico para diagnóstico precoce da aspergilose em pinguins em cativeiro Cabana, Ângela Leitzke Veterinária Sphenisciformes Cativeiro Aspergillus sp Anticorpos Imunodifusão IDGA Veterinary Sphenisciformes Captivity Aspergillus sp Antibody Immunodiffusion AGID CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Monitoramento sorológico para diagnóstico precoce da aspergilose em pinguins em cativeiro |
title_full |
Monitoramento sorológico para diagnóstico precoce da aspergilose em pinguins em cativeiro |
title_fullStr |
Monitoramento sorológico para diagnóstico precoce da aspergilose em pinguins em cativeiro |
title_full_unstemmed |
Monitoramento sorológico para diagnóstico precoce da aspergilose em pinguins em cativeiro |
title_sort |
Monitoramento sorológico para diagnóstico precoce da aspergilose em pinguins em cativeiro |
author |
Cabana, Ângela Leitzke |
author_facet |
Cabana, Ângela Leitzke |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/3380926755008181 |
dc.contributor.advisorLattes.por.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4799478H9 |
dc.contributor.referees1.pt_BR.fl_str_mv |
Vargas, Gilberto D ávila |
dc.contributor.referees1ID.por.fl_str_mv |
|
dc.contributor.referees1Lattes.por.fl_str_mv |
http://lattes.cnpq.br/2905761117990157 |
dc.contributor.advisor1.fl_str_mv |
Meireles, Mario Carlos Araújo |
dc.contributor.author.fl_str_mv |
Cabana, Ângela Leitzke |
contributor_str_mv |
Meireles, Mario Carlos Araújo |
dc.subject.por.fl_str_mv |
Veterinária Sphenisciformes Cativeiro Aspergillus sp Anticorpos Imunodifusão IDGA |
topic |
Veterinária Sphenisciformes Cativeiro Aspergillus sp Anticorpos Imunodifusão IDGA Veterinary Sphenisciformes Captivity Aspergillus sp Antibody Immunodiffusion AGID CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.eng.fl_str_mv |
Veterinary Sphenisciformes Captivity Aspergillus sp Antibody Immunodiffusion AGID |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
Aspergillosis is a leading cause of death in captive penguins. Though being a known problem for decades, most reported cases are still only confirmed in post-mortem examinations, requiring further studies on methods for in vivo diagnosis of the disease. This study aimed to evaluate the efficacy of detection of anti-Aspergillus fumigatus antibodies through the performance by serological monitoring with agar gel double radial immunodiffusion (AGID) for in vivo diagnosis of aspergillosis in captive penguins. The study was performed including Magellanic penguins in a rehabilitation program at Center for Recovery of Marine Animals (CRAM/FURG) in the period from 2009 to 2011. We included 134 Magellanic penguins (Spheniscus magellanicus) in rehabilitation at CRAM, which were monitored by AGID weekly until its final destination (death or release) totaling 660 studied serum samples. The serological monitoring was performed in order to detect anti-Aspergillus fumigatus antibodies using commercial antibodies and antigen. All animals were clinically monitored and post-mortem examinations were performed on penguins that died during the study period. A total of 28% (37/134) penguin died, 89.2% (33/37) of aspergillosis, 11% (4/37) of other causes and 97 were released. From the 33 animals with proven aspergillosis, 21 had anti-A. fumigatus antibodies by AGID, being the average interval between death and the positive AGID result of 16.4 days. Twelve seronegative animals eventually died of aspergillosis. The rates of sensitivity and specificity were 63.6% and 95%, respectively, and positive and negative predictive values were 80.7% and 88.9% respectively. These data demonstrate that serological monitoring for the detection of antibodies by AGID may be a useful and important tool for the diagnosis of aspergillosis in penguins. As our study with AGID in penguins is pioneer, more studies are required concerning the prognosis in cases of aspergillosis in penguins whose therapeutic intervention is based on diagnosis by AGID, considering that the average period between the positive result of this test and the death of the animal was less than one month. Key-words: Sphenisciformes. Captivity |
publishDate |
2013 |
dc.date.available.fl_str_mv |
2013-08-21 2014-08-20T14:37:52Z |
dc.date.issued.fl_str_mv |
2013-02-28 |
dc.date.accessioned.fl_str_mv |
2014-08-20T14:37:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CABANA, Ângela Leitzke. Monitoramento sorológico para diagnostic precoce da aspergilose em pinguins em cativeiro. 2013. 110 f. Dissertação (Mestrado em Veterinária) - Universidade Federal de Pelotas, Pelotas, 2013. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufpel.edu.br/handle/ri/2490 |
identifier_str_mv |
CABANA, Ângela Leitzke. Monitoramento sorológico para diagnostic precoce da aspergilose em pinguins em cativeiro. 2013. 110 f. Dissertação (Mestrado em Veterinária) - Universidade Federal de Pelotas, Pelotas, 2013. |
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http://repositorio.ufpel.edu.br/handle/ri/2490 |
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Universidade Federal de Pelotas |
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Programa de Pós-Graduação em Veterinária |
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UFPel |
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BR |
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Veterinária |
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Universidade Federal de Pelotas |
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