Detection of illegal estrogen administration through immunohistochemical markers in the bovine prostate

Detalhes bibliográficos
Autor(a) principal: Fernández,Patricia Elena
Data de Publicação: 1999
Outros Autores: Sanguinetti,Héctor Ramón, Portiansky,Enrique Leo, Barbeito,Claudio Gustavo, Gimeno,Eduardo Juan
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Veterinária Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X1999000300008
Resumo: The immunodetection of diverse cell markers was evaluated in prostatic samples from bullocks, and bullocks showing epithelial hyperplasia-metaplasia, with oestrogen-induced changes, and in experimental samples from bullocks inoculated with dietylstilbestrol (DES). Antigen-retrieval procedures allowed the use of tissues that had been fixed in formalin for long periods. Three tissue markers were chosen for the study: cytokeratins 13 and 16, vimentin and desmin. Monoclonal antibody K8.12 (specific for cytokeratins 13 and 16) stained basal cells and hyperplastic-metaplastic epithelium; monoclonal antivimentin, and desmin, allowed the definition of fibromuscular changes.
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spelling Detection of illegal estrogen administration through immunohistochemical markers in the bovine prostateBovine prostatecell markersimmunohistochemistryoestrogenThe immunodetection of diverse cell markers was evaluated in prostatic samples from bullocks, and bullocks showing epithelial hyperplasia-metaplasia, with oestrogen-induced changes, and in experimental samples from bullocks inoculated with dietylstilbestrol (DES). Antigen-retrieval procedures allowed the use of tissues that had been fixed in formalin for long periods. Three tissue markers were chosen for the study: cytokeratins 13 and 16, vimentin and desmin. Monoclonal antibody K8.12 (specific for cytokeratins 13 and 16) stained basal cells and hyperplastic-metaplastic epithelium; monoclonal antivimentin, and desmin, allowed the definition of fibromuscular changes.Colégio Brasileiro de Patologia Animal - CBPA1999-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X1999000300008Pesquisa Veterinária Brasileira v.19 n.3-4 1999reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/S0100-736X1999000300008info:eu-repo/semantics/openAccessFernández,Patricia ElenaSanguinetti,Héctor RamónPortiansky,Enrique LeoBarbeito,Claudio GustavoGimeno,Eduardo Juaneng2000-02-25T00:00:00Zoai:scielo:S0100-736X1999000300008Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2000-02-25T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false
dc.title.none.fl_str_mv Detection of illegal estrogen administration through immunohistochemical markers in the bovine prostate
title Detection of illegal estrogen administration through immunohistochemical markers in the bovine prostate
spellingShingle Detection of illegal estrogen administration through immunohistochemical markers in the bovine prostate
Fernández,Patricia Elena
Bovine prostate
cell markers
immunohistochemistry
oestrogen
title_short Detection of illegal estrogen administration through immunohistochemical markers in the bovine prostate
title_full Detection of illegal estrogen administration through immunohistochemical markers in the bovine prostate
title_fullStr Detection of illegal estrogen administration through immunohistochemical markers in the bovine prostate
title_full_unstemmed Detection of illegal estrogen administration through immunohistochemical markers in the bovine prostate
title_sort Detection of illegal estrogen administration through immunohistochemical markers in the bovine prostate
author Fernández,Patricia Elena
author_facet Fernández,Patricia Elena
Sanguinetti,Héctor Ramón
Portiansky,Enrique Leo
Barbeito,Claudio Gustavo
Gimeno,Eduardo Juan
author_role author
author2 Sanguinetti,Héctor Ramón
Portiansky,Enrique Leo
Barbeito,Claudio Gustavo
Gimeno,Eduardo Juan
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Fernández,Patricia Elena
Sanguinetti,Héctor Ramón
Portiansky,Enrique Leo
Barbeito,Claudio Gustavo
Gimeno,Eduardo Juan
dc.subject.por.fl_str_mv Bovine prostate
cell markers
immunohistochemistry
oestrogen
topic Bovine prostate
cell markers
immunohistochemistry
oestrogen
description The immunodetection of diverse cell markers was evaluated in prostatic samples from bullocks, and bullocks showing epithelial hyperplasia-metaplasia, with oestrogen-induced changes, and in experimental samples from bullocks inoculated with dietylstilbestrol (DES). Antigen-retrieval procedures allowed the use of tissues that had been fixed in formalin for long periods. Three tissue markers were chosen for the study: cytokeratins 13 and 16, vimentin and desmin. Monoclonal antibody K8.12 (specific for cytokeratins 13 and 16) stained basal cells and hyperplastic-metaplastic epithelium; monoclonal antivimentin, and desmin, allowed the definition of fibromuscular changes.
publishDate 1999
dc.date.none.fl_str_mv 1999-07-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X1999000300008
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X1999000300008
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-736X1999000300008
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
dc.source.none.fl_str_mv Pesquisa Veterinária Brasileira v.19 n.3-4 1999
reponame:Pesquisa Veterinária Brasileira (Online)
instname:Colégio Brasileiro de Patologia Animal (CBPA)
instacron:EMBRAPA
instname_str Colégio Brasileiro de Patologia Animal (CBPA)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Veterinária Brasileira (Online)
collection Pesquisa Veterinária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)
repository.mail.fl_str_mv colegio@cbpa.org.br||pvb@pvb.com.br
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