Tumor-associated macrophage is correlated with survival and SOCS protein expression in canine mammary carcinoma

Detalhes bibliográficos
Autor(a) principal: Vieira-Filho,Carlos H.C.
Data de Publicação: 2018
Outros Autores: Barrouin-Melo,Stella M., Damasceno,Karine A., Araújo,Márcio S.S., Borges,Natalie F., Silva,Fabiana L., Cassali,Geovanni D., Estrela-Lima,Alessandra
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Veterinária Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2018001001972
Resumo: ABSTRACT: The inflammatory infiltrate in the tumor microenvironment, particularly in mammary tumors, has aroused great interest in oncology, to play different roles in the progression or tumor regression dependent on the types and cell subsets involved. The present study aimed to evaluate (1) the occurrence and intensity of macrophage infiltration in the mammary carcinoma microenvironment, (2) the expression of SOCS1 and SOCS3 proteins in tumor associated macrophages, (3) any association between these parameters and tumor development, as well as survival rates in female dogs. Twenty-two female dogs diagnosed as carcinoma arising in a mixed tumor (CMT) by histopathology were divided into two groups following mastectomy: dogs without metastasis (CMT(-)=11) and those with metastasis (CMT(+)=11). The following parameters were analyzed: tumor size, lymph node metastasis, clinical stage, histological grade, distribution and intensity of inflammatory infiltrate, tumor macrophage quantification by immunohistochemical analysis of SOCS1 and SOCS3 expression, and immunophenotyping of peripheral blood leukocytes by flow cytometry. Dogs with the higher proportions of macrophages in the inflammatory infiltrate (≥400/tumor) also had higher survival rates in comparison with dogs with less macrophages. Immunostaining revealed higher proportions of SOCS3-positive macrophages in dogs without lymph node metastasis, while SOCS1-positive macrophages were predominant in dogs with metastasis (p<0.05). Multivariate analysis found associations between survival rate and clinical staging (p=0.025), histological grade (p=0.007), and the expression of MHC-CI in circulating monocytes (p=0.018). Higher SOCS3 expression in activated macrophages within the inflammatory infiltrate were considered indicative of an antitumor immune response, improved clinicopathological parameters and longer survival, whereas SOCS1-related activation was associated with tumor progression, metastasis development and reduced survival in female dogs with mammary carcinomas.
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spelling Tumor-associated macrophage is correlated with survival and SOCS protein expression in canine mammary carcinomaCanine mammary carcinomatumordogmacrophagescytokinedogspathologyABSTRACT: The inflammatory infiltrate in the tumor microenvironment, particularly in mammary tumors, has aroused great interest in oncology, to play different roles in the progression or tumor regression dependent on the types and cell subsets involved. The present study aimed to evaluate (1) the occurrence and intensity of macrophage infiltration in the mammary carcinoma microenvironment, (2) the expression of SOCS1 and SOCS3 proteins in tumor associated macrophages, (3) any association between these parameters and tumor development, as well as survival rates in female dogs. Twenty-two female dogs diagnosed as carcinoma arising in a mixed tumor (CMT) by histopathology were divided into two groups following mastectomy: dogs without metastasis (CMT(-)=11) and those with metastasis (CMT(+)=11). The following parameters were analyzed: tumor size, lymph node metastasis, clinical stage, histological grade, distribution and intensity of inflammatory infiltrate, tumor macrophage quantification by immunohistochemical analysis of SOCS1 and SOCS3 expression, and immunophenotyping of peripheral blood leukocytes by flow cytometry. Dogs with the higher proportions of macrophages in the inflammatory infiltrate (≥400/tumor) also had higher survival rates in comparison with dogs with less macrophages. Immunostaining revealed higher proportions of SOCS3-positive macrophages in dogs without lymph node metastasis, while SOCS1-positive macrophages were predominant in dogs with metastasis (p<0.05). Multivariate analysis found associations between survival rate and clinical staging (p=0.025), histological grade (p=0.007), and the expression of MHC-CI in circulating monocytes (p=0.018). Higher SOCS3 expression in activated macrophages within the inflammatory infiltrate were considered indicative of an antitumor immune response, improved clinicopathological parameters and longer survival, whereas SOCS1-related activation was associated with tumor progression, metastasis development and reduced survival in female dogs with mammary carcinomas.Colégio Brasileiro de Patologia Animal - CBPA2018-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2018001001972Pesquisa Veterinária Brasileira v.38 n.10 2018reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/1678-5150-pvb-5638info:eu-repo/semantics/openAccessVieira-Filho,Carlos H.C.Barrouin-Melo,Stella M.Damasceno,Karine A.Araújo,Márcio S.S.Borges,Natalie F.Silva,Fabiana L.Cassali,Geovanni D.Estrela-Lima,Alessandraeng2018-11-26T00:00:00Zoai:scielo:S0100-736X2018001001972Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2018-11-26T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false
dc.title.none.fl_str_mv Tumor-associated macrophage is correlated with survival and SOCS protein expression in canine mammary carcinoma
title Tumor-associated macrophage is correlated with survival and SOCS protein expression in canine mammary carcinoma
spellingShingle Tumor-associated macrophage is correlated with survival and SOCS protein expression in canine mammary carcinoma
Vieira-Filho,Carlos H.C.
Canine mammary carcinoma
tumor
dog
macrophages
cytokine
dogs
pathology
title_short Tumor-associated macrophage is correlated with survival and SOCS protein expression in canine mammary carcinoma
title_full Tumor-associated macrophage is correlated with survival and SOCS protein expression in canine mammary carcinoma
title_fullStr Tumor-associated macrophage is correlated with survival and SOCS protein expression in canine mammary carcinoma
title_full_unstemmed Tumor-associated macrophage is correlated with survival and SOCS protein expression in canine mammary carcinoma
title_sort Tumor-associated macrophage is correlated with survival and SOCS protein expression in canine mammary carcinoma
author Vieira-Filho,Carlos H.C.
author_facet Vieira-Filho,Carlos H.C.
Barrouin-Melo,Stella M.
Damasceno,Karine A.
Araújo,Márcio S.S.
Borges,Natalie F.
Silva,Fabiana L.
Cassali,Geovanni D.
Estrela-Lima,Alessandra
author_role author
author2 Barrouin-Melo,Stella M.
Damasceno,Karine A.
Araújo,Márcio S.S.
Borges,Natalie F.
Silva,Fabiana L.
Cassali,Geovanni D.
Estrela-Lima,Alessandra
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Vieira-Filho,Carlos H.C.
Barrouin-Melo,Stella M.
Damasceno,Karine A.
Araújo,Márcio S.S.
Borges,Natalie F.
Silva,Fabiana L.
Cassali,Geovanni D.
Estrela-Lima,Alessandra
dc.subject.por.fl_str_mv Canine mammary carcinoma
tumor
dog
macrophages
cytokine
dogs
pathology
topic Canine mammary carcinoma
tumor
dog
macrophages
cytokine
dogs
pathology
description ABSTRACT: The inflammatory infiltrate in the tumor microenvironment, particularly in mammary tumors, has aroused great interest in oncology, to play different roles in the progression or tumor regression dependent on the types and cell subsets involved. The present study aimed to evaluate (1) the occurrence and intensity of macrophage infiltration in the mammary carcinoma microenvironment, (2) the expression of SOCS1 and SOCS3 proteins in tumor associated macrophages, (3) any association between these parameters and tumor development, as well as survival rates in female dogs. Twenty-two female dogs diagnosed as carcinoma arising in a mixed tumor (CMT) by histopathology were divided into two groups following mastectomy: dogs without metastasis (CMT(-)=11) and those with metastasis (CMT(+)=11). The following parameters were analyzed: tumor size, lymph node metastasis, clinical stage, histological grade, distribution and intensity of inflammatory infiltrate, tumor macrophage quantification by immunohistochemical analysis of SOCS1 and SOCS3 expression, and immunophenotyping of peripheral blood leukocytes by flow cytometry. Dogs with the higher proportions of macrophages in the inflammatory infiltrate (≥400/tumor) also had higher survival rates in comparison with dogs with less macrophages. Immunostaining revealed higher proportions of SOCS3-positive macrophages in dogs without lymph node metastasis, while SOCS1-positive macrophages were predominant in dogs with metastasis (p<0.05). Multivariate analysis found associations between survival rate and clinical staging (p=0.025), histological grade (p=0.007), and the expression of MHC-CI in circulating monocytes (p=0.018). Higher SOCS3 expression in activated macrophages within the inflammatory infiltrate were considered indicative of an antitumor immune response, improved clinicopathological parameters and longer survival, whereas SOCS1-related activation was associated with tumor progression, metastasis development and reduced survival in female dogs with mammary carcinomas.
publishDate 2018
dc.date.none.fl_str_mv 2018-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2018001001972
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2018001001972
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-5150-pvb-5638
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
dc.source.none.fl_str_mv Pesquisa Veterinária Brasileira v.38 n.10 2018
reponame:Pesquisa Veterinária Brasileira (Online)
instname:Colégio Brasileiro de Patologia Animal (CBPA)
instacron:EMBRAPA
instname_str Colégio Brasileiro de Patologia Animal (CBPA)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Veterinária Brasileira (Online)
collection Pesquisa Veterinária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)
repository.mail.fl_str_mv colegio@cbpa.org.br||pvb@pvb.com.br
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