Expression of LIN28A/B and Let-7 miRNAs in canine mammary carcinomas
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência Rural |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782022001000551 |
Resumo: | ABSTRACT: LIN28 is a RNA-binding protein including two highly conserved homologous, LIN28A and LIN28B. Proto-oncogenes such as LIN28A and LIN28B are generally targeted by the let-7 miRNAs in different types of human cancers. Here, we determined the expression of LIN28A in canine mammary tumor samples and the LIN28/let-7 pathway in canine mammary cell lines. In those cell lines, we identified a functional LIN28/let-7 pathway which exhibited high expression of let-7 members and low expression of its targets, including LIN28A and LIN28B. However, the mammary carcinoma tissue samples showed a frequent expression of LIN28A being expressed mainly in the epithelial cells. No association was observed between LIN28A expression and histopathological classification and grade, TNM and survival time. Our results suggested a possible role of the LIN28A protein in the development of canine mammary carcinomas due to the high frequency observed in the tumor samples (28 of 32). The in vitro experiments suggested that the LIN28/let-7 pathway is active in the tumor cells evaluated. However, more studies are necessary to elucidate the exact role of LIN28/let-7 pathway in canine mammary carcinomas. |
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Ciência rural (Online) |
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Expression of LIN28A/B and Let-7 miRNAs in canine mammary carcinomascanine mammary carcinomasLIN28let-7veterinary oncologyABSTRACT: LIN28 is a RNA-binding protein including two highly conserved homologous, LIN28A and LIN28B. Proto-oncogenes such as LIN28A and LIN28B are generally targeted by the let-7 miRNAs in different types of human cancers. Here, we determined the expression of LIN28A in canine mammary tumor samples and the LIN28/let-7 pathway in canine mammary cell lines. In those cell lines, we identified a functional LIN28/let-7 pathway which exhibited high expression of let-7 members and low expression of its targets, including LIN28A and LIN28B. However, the mammary carcinoma tissue samples showed a frequent expression of LIN28A being expressed mainly in the epithelial cells. No association was observed between LIN28A expression and histopathological classification and grade, TNM and survival time. Our results suggested a possible role of the LIN28A protein in the development of canine mammary carcinomas due to the high frequency observed in the tumor samples (28 of 32). The in vitro experiments suggested that the LIN28/let-7 pathway is active in the tumor cells evaluated. However, more studies are necessary to elucidate the exact role of LIN28/let-7 pathway in canine mammary carcinomas.Universidade Federal de Santa Maria2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782022001000551Ciência Rural v.52 n.10 2022reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20210171info:eu-repo/semantics/openAccessGuilharducci,Raquel LozanoXavier,Pedro Luiz PorfirioSilveira,Juliano Coelho daCordeiro,Yonara de GouveiaBiondi,Luiz RobertoStrefezzi,Ricardo de FranciscoFukumasu,Heidgeeng2022-03-09T00:00:00ZRevista |
dc.title.none.fl_str_mv |
Expression of LIN28A/B and Let-7 miRNAs in canine mammary carcinomas |
title |
Expression of LIN28A/B and Let-7 miRNAs in canine mammary carcinomas |
spellingShingle |
Expression of LIN28A/B and Let-7 miRNAs in canine mammary carcinomas Guilharducci,Raquel Lozano canine mammary carcinomas LIN28 let-7 veterinary oncology |
title_short |
Expression of LIN28A/B and Let-7 miRNAs in canine mammary carcinomas |
title_full |
Expression of LIN28A/B and Let-7 miRNAs in canine mammary carcinomas |
title_fullStr |
Expression of LIN28A/B and Let-7 miRNAs in canine mammary carcinomas |
title_full_unstemmed |
Expression of LIN28A/B and Let-7 miRNAs in canine mammary carcinomas |
title_sort |
Expression of LIN28A/B and Let-7 miRNAs in canine mammary carcinomas |
author |
Guilharducci,Raquel Lozano |
author_facet |
Guilharducci,Raquel Lozano Xavier,Pedro Luiz Porfirio Silveira,Juliano Coelho da Cordeiro,Yonara de Gouveia Biondi,Luiz Roberto Strefezzi,Ricardo de Francisco Fukumasu,Heidge |
author_role |
author |
author2 |
Xavier,Pedro Luiz Porfirio Silveira,Juliano Coelho da Cordeiro,Yonara de Gouveia Biondi,Luiz Roberto Strefezzi,Ricardo de Francisco Fukumasu,Heidge |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Guilharducci,Raquel Lozano Xavier,Pedro Luiz Porfirio Silveira,Juliano Coelho da Cordeiro,Yonara de Gouveia Biondi,Luiz Roberto Strefezzi,Ricardo de Francisco Fukumasu,Heidge |
dc.subject.por.fl_str_mv |
canine mammary carcinomas LIN28 let-7 veterinary oncology |
topic |
canine mammary carcinomas LIN28 let-7 veterinary oncology |
description |
ABSTRACT: LIN28 is a RNA-binding protein including two highly conserved homologous, LIN28A and LIN28B. Proto-oncogenes such as LIN28A and LIN28B are generally targeted by the let-7 miRNAs in different types of human cancers. Here, we determined the expression of LIN28A in canine mammary tumor samples and the LIN28/let-7 pathway in canine mammary cell lines. In those cell lines, we identified a functional LIN28/let-7 pathway which exhibited high expression of let-7 members and low expression of its targets, including LIN28A and LIN28B. However, the mammary carcinoma tissue samples showed a frequent expression of LIN28A being expressed mainly in the epithelial cells. No association was observed between LIN28A expression and histopathological classification and grade, TNM and survival time. Our results suggested a possible role of the LIN28A protein in the development of canine mammary carcinomas due to the high frequency observed in the tumor samples (28 of 32). The in vitro experiments suggested that the LIN28/let-7 pathway is active in the tumor cells evaluated. However, more studies are necessary to elucidate the exact role of LIN28/let-7 pathway in canine mammary carcinomas. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782022001000551 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782022001000551 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-8478cr20210171 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural v.52 n.10 2022 reponame:Ciência Rural instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Rural |
collection |
Ciência Rural |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1749140557039403008 |