Expression of LIN28A/B and Let-7 miRNAs in canine mammary carcinomas

Detalhes bibliográficos
Autor(a) principal: Guilharducci,Raquel Lozano
Data de Publicação: 2022
Outros Autores: Xavier,Pedro Luiz Porfirio, Silveira,Juliano Coelho da, Cordeiro,Yonara de Gouveia, Biondi,Luiz Roberto, Strefezzi,Ricardo de Francisco, Fukumasu,Heidge
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Ciência Rural
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782022001000551
Resumo: ABSTRACT: LIN28 is a RNA-binding protein including two highly conserved homologous, LIN28A and LIN28B. Proto-oncogenes such as LIN28A and LIN28B are generally targeted by the let-7 miRNAs in different types of human cancers. Here, we determined the expression of LIN28A in canine mammary tumor samples and the LIN28/let-7 pathway in canine mammary cell lines. In those cell lines, we identified a functional LIN28/let-7 pathway which exhibited high expression of let-7 members and low expression of its targets, including LIN28A and LIN28B. However, the mammary carcinoma tissue samples showed a frequent expression of LIN28A being expressed mainly in the epithelial cells. No association was observed between LIN28A expression and histopathological classification and grade, TNM and survival time. Our results suggested a possible role of the LIN28A protein in the development of canine mammary carcinomas due to the high frequency observed in the tumor samples (28 of 32). The in vitro experiments suggested that the LIN28/let-7 pathway is active in the tumor cells evaluated. However, more studies are necessary to elucidate the exact role of LIN28/let-7 pathway in canine mammary carcinomas.
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spelling Expression of LIN28A/B and Let-7 miRNAs in canine mammary carcinomascanine mammary carcinomasLIN28let-7veterinary oncologyABSTRACT: LIN28 is a RNA-binding protein including two highly conserved homologous, LIN28A and LIN28B. Proto-oncogenes such as LIN28A and LIN28B are generally targeted by the let-7 miRNAs in different types of human cancers. Here, we determined the expression of LIN28A in canine mammary tumor samples and the LIN28/let-7 pathway in canine mammary cell lines. In those cell lines, we identified a functional LIN28/let-7 pathway which exhibited high expression of let-7 members and low expression of its targets, including LIN28A and LIN28B. However, the mammary carcinoma tissue samples showed a frequent expression of LIN28A being expressed mainly in the epithelial cells. No association was observed between LIN28A expression and histopathological classification and grade, TNM and survival time. Our results suggested a possible role of the LIN28A protein in the development of canine mammary carcinomas due to the high frequency observed in the tumor samples (28 of 32). The in vitro experiments suggested that the LIN28/let-7 pathway is active in the tumor cells evaluated. However, more studies are necessary to elucidate the exact role of LIN28/let-7 pathway in canine mammary carcinomas.Universidade Federal de Santa Maria2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782022001000551Ciência Rural v.52 n.10 2022reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20210171info:eu-repo/semantics/openAccessGuilharducci,Raquel LozanoXavier,Pedro Luiz PorfirioSilveira,Juliano Coelho daCordeiro,Yonara de GouveiaBiondi,Luiz RobertoStrefezzi,Ricardo de FranciscoFukumasu,Heidgeeng2022-03-09T00:00:00ZRevista
dc.title.none.fl_str_mv Expression of LIN28A/B and Let-7 miRNAs in canine mammary carcinomas
title Expression of LIN28A/B and Let-7 miRNAs in canine mammary carcinomas
spellingShingle Expression of LIN28A/B and Let-7 miRNAs in canine mammary carcinomas
Guilharducci,Raquel Lozano
canine mammary carcinomas
LIN28
let-7
veterinary oncology
title_short Expression of LIN28A/B and Let-7 miRNAs in canine mammary carcinomas
title_full Expression of LIN28A/B and Let-7 miRNAs in canine mammary carcinomas
title_fullStr Expression of LIN28A/B and Let-7 miRNAs in canine mammary carcinomas
title_full_unstemmed Expression of LIN28A/B and Let-7 miRNAs in canine mammary carcinomas
title_sort Expression of LIN28A/B and Let-7 miRNAs in canine mammary carcinomas
author Guilharducci,Raquel Lozano
author_facet Guilharducci,Raquel Lozano
Xavier,Pedro Luiz Porfirio
Silveira,Juliano Coelho da
Cordeiro,Yonara de Gouveia
Biondi,Luiz Roberto
Strefezzi,Ricardo de Francisco
Fukumasu,Heidge
author_role author
author2 Xavier,Pedro Luiz Porfirio
Silveira,Juliano Coelho da
Cordeiro,Yonara de Gouveia
Biondi,Luiz Roberto
Strefezzi,Ricardo de Francisco
Fukumasu,Heidge
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Guilharducci,Raquel Lozano
Xavier,Pedro Luiz Porfirio
Silveira,Juliano Coelho da
Cordeiro,Yonara de Gouveia
Biondi,Luiz Roberto
Strefezzi,Ricardo de Francisco
Fukumasu,Heidge
dc.subject.por.fl_str_mv canine mammary carcinomas
LIN28
let-7
veterinary oncology
topic canine mammary carcinomas
LIN28
let-7
veterinary oncology
description ABSTRACT: LIN28 is a RNA-binding protein including two highly conserved homologous, LIN28A and LIN28B. Proto-oncogenes such as LIN28A and LIN28B are generally targeted by the let-7 miRNAs in different types of human cancers. Here, we determined the expression of LIN28A in canine mammary tumor samples and the LIN28/let-7 pathway in canine mammary cell lines. In those cell lines, we identified a functional LIN28/let-7 pathway which exhibited high expression of let-7 members and low expression of its targets, including LIN28A and LIN28B. However, the mammary carcinoma tissue samples showed a frequent expression of LIN28A being expressed mainly in the epithelial cells. No association was observed between LIN28A expression and histopathological classification and grade, TNM and survival time. Our results suggested a possible role of the LIN28A protein in the development of canine mammary carcinomas due to the high frequency observed in the tumor samples (28 of 32). The in vitro experiments suggested that the LIN28/let-7 pathway is active in the tumor cells evaluated. However, more studies are necessary to elucidate the exact role of LIN28/let-7 pathway in canine mammary carcinomas.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782022001000551
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782022001000551
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0103-8478cr20210171
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Rural v.52 n.10 2022
reponame:Ciência Rural
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Rural
collection Ciência Rural
repository.name.fl_str_mv
repository.mail.fl_str_mv
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