Serology, polymerase chain reaction and histopathology for leptospirosis in samples collected at slaughter from dairy cows of Parnaiba region, state of Piauí, Brazil
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2011 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Pesquisa Veterinária Brasileira (Online) |
| Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2011001000005 |
Resumo: | The presence of anti leptospiral agglutinins (microscopic agglutination test - MAT) and DNA of leptospires was investigated in the kidney and urine (Polymerase Chain Reaction - PCR) in samples collected at the time of slaughter of cattle originating from the dairy basin of Parnaíba, Piauí, Brazil, as also the lesions in kidney, lung, liver, uterus, ovary and placenta (histopathology and immunohistochemistry). In the MAT, Hardjo was the predominant serovar with the highest number of reagent animals for the strain Hardjobovis/Sponselee. Anti-leptospiral antigens were scored in epithelial cells, interstitial vascular endothelium, endothelium of glomerular capillaries and Bowman's capsule of 20 positive animals. Inflammatory cells were more common in the kidney. PCR was positive in urine and kidney tissue |
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Serology, polymerase chain reaction and histopathology for leptospirosis in samples collected at slaughter from dairy cows of Parnaiba region, state of Piauí, BrazilLeptospirosisimmunohistochemistryPCRcattleThe presence of anti leptospiral agglutinins (microscopic agglutination test - MAT) and DNA of leptospires was investigated in the kidney and urine (Polymerase Chain Reaction - PCR) in samples collected at the time of slaughter of cattle originating from the dairy basin of Parnaíba, Piauí, Brazil, as also the lesions in kidney, lung, liver, uterus, ovary and placenta (histopathology and immunohistochemistry). In the MAT, Hardjo was the predominant serovar with the highest number of reagent animals for the strain Hardjobovis/Sponselee. Anti-leptospiral antigens were scored in epithelial cells, interstitial vascular endothelium, endothelium of glomerular capillaries and Bowman's capsule of 20 positive animals. Inflammatory cells were more common in the kidney. PCR was positive in urine and kidney tissueColégio Brasileiro de Patologia Animal - CBPA2011-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2011001000005Pesquisa Veterinária Brasileira v.31 n.10 2011reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/S0100-736X2011001000005info:eu-repo/semantics/openAccessMineiro,Ana Lys Bezerra BarrradasVieira,Rômulo JoséCosta,Érica AzevedoSantos,Renato LimaGonçalves,Larissa Maria FeitosaCarvalho,Sônia MariaBomfim,Maria Rosa QuaresmaCosta,Francisco Assis Limaeng2011-11-29T00:00:00Zoai:scielo:S0100-736X2011001000005Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2011-11-29T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false |
| dc.title.none.fl_str_mv |
Serology, polymerase chain reaction and histopathology for leptospirosis in samples collected at slaughter from dairy cows of Parnaiba region, state of Piauí, Brazil |
| title |
Serology, polymerase chain reaction and histopathology for leptospirosis in samples collected at slaughter from dairy cows of Parnaiba region, state of Piauí, Brazil |
| spellingShingle |
Serology, polymerase chain reaction and histopathology for leptospirosis in samples collected at slaughter from dairy cows of Parnaiba region, state of Piauí, Brazil Mineiro,Ana Lys Bezerra Barrradas Leptospirosis immunohistochemistry PCR cattle |
| title_short |
Serology, polymerase chain reaction and histopathology for leptospirosis in samples collected at slaughter from dairy cows of Parnaiba region, state of Piauí, Brazil |
| title_full |
Serology, polymerase chain reaction and histopathology for leptospirosis in samples collected at slaughter from dairy cows of Parnaiba region, state of Piauí, Brazil |
| title_fullStr |
Serology, polymerase chain reaction and histopathology for leptospirosis in samples collected at slaughter from dairy cows of Parnaiba region, state of Piauí, Brazil |
| title_full_unstemmed |
Serology, polymerase chain reaction and histopathology for leptospirosis in samples collected at slaughter from dairy cows of Parnaiba region, state of Piauí, Brazil |
| title_sort |
Serology, polymerase chain reaction and histopathology for leptospirosis in samples collected at slaughter from dairy cows of Parnaiba region, state of Piauí, Brazil |
| author |
Mineiro,Ana Lys Bezerra Barrradas |
| author_facet |
Mineiro,Ana Lys Bezerra Barrradas Vieira,Rômulo José Costa,Érica Azevedo Santos,Renato Lima Gonçalves,Larissa Maria Feitosa Carvalho,Sônia Maria Bomfim,Maria Rosa Quaresma Costa,Francisco Assis Lima |
| author_role |
author |
| author2 |
Vieira,Rômulo José Costa,Érica Azevedo Santos,Renato Lima Gonçalves,Larissa Maria Feitosa Carvalho,Sônia Maria Bomfim,Maria Rosa Quaresma Costa,Francisco Assis Lima |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Mineiro,Ana Lys Bezerra Barrradas Vieira,Rômulo José Costa,Érica Azevedo Santos,Renato Lima Gonçalves,Larissa Maria Feitosa Carvalho,Sônia Maria Bomfim,Maria Rosa Quaresma Costa,Francisco Assis Lima |
| dc.subject.por.fl_str_mv |
Leptospirosis immunohistochemistry PCR cattle |
| topic |
Leptospirosis immunohistochemistry PCR cattle |
| description |
The presence of anti leptospiral agglutinins (microscopic agglutination test - MAT) and DNA of leptospires was investigated in the kidney and urine (Polymerase Chain Reaction - PCR) in samples collected at the time of slaughter of cattle originating from the dairy basin of Parnaíba, Piauí, Brazil, as also the lesions in kidney, lung, liver, uterus, ovary and placenta (histopathology and immunohistochemistry). In the MAT, Hardjo was the predominant serovar with the highest number of reagent animals for the strain Hardjobovis/Sponselee. Anti-leptospiral antigens were scored in epithelial cells, interstitial vascular endothelium, endothelium of glomerular capillaries and Bowman's capsule of 20 positive animals. Inflammatory cells were more common in the kidney. PCR was positive in urine and kidney tissue |
| publishDate |
2011 |
| dc.date.none.fl_str_mv |
2011-10-01 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2011001000005 |
| url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2011001000005 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
10.1590/S0100-736X2011001000005 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
text/html |
| dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
| publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
| dc.source.none.fl_str_mv |
Pesquisa Veterinária Brasileira v.31 n.10 2011 reponame:Pesquisa Veterinária Brasileira (Online) instname:Colégio Brasileiro de Patologia Animal (CBPA) instacron:EMBRAPA |
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Colégio Brasileiro de Patologia Animal (CBPA) |
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EMBRAPA |
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EMBRAPA |
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Pesquisa Veterinária Brasileira (Online) |
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Pesquisa Veterinária Brasileira (Online) |
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Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA) |
| repository.mail.fl_str_mv |
colegio@cbpa.org.br||pvb@pvb.com.br |
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1754122231373365248 |