Culture of equine bone marrow mononuclear fraction and adipose tissue-derived stromal vascular fraction cells in different media

Detalhes bibliográficos
Autor(a) principal: Ribeiro,Gesiane
Data de Publicação: 2013
Outros Autores: Massoco,Cristina O., Lacerda Neto,José Corrêa de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Veterinária Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2013001300004
Resumo: The objective of this study was to evaluate the culture of equine bone marrow mononuclear fraction and adipose tissue - derived stromal vascular fraction cells in two different cell culture media. Five adult horses were submitted to bone marrow aspiration from the sternum, and then from the adipose tissue of the gluteal region near the base of the tail. Mononuclear fraction and stromal vascular fraction were isolated from the samples and cultivated in DMEM medium supplemented with 10% fetal bovine serum or in AIM-V medium. The cultures were observed once a week with an inverted microscope, to perform a qualitative analysis of the morphology of the cells as well as the general appearance of the cell culture. Colony-forming units (CFU) were counted on days 5, 15 and 25 of cell culture. During the first week of culture, differences were observed between the samples from the same source maintained in different culture media. The number of colonies was significantly higher in samples of bone marrow in relation to samples of adipose tissue.
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spelling Culture of equine bone marrow mononuclear fraction and adipose tissue-derived stromal vascular fraction cells in different mediaHorsesbone marrowadipose tissueculture mediumThe objective of this study was to evaluate the culture of equine bone marrow mononuclear fraction and adipose tissue - derived stromal vascular fraction cells in two different cell culture media. Five adult horses were submitted to bone marrow aspiration from the sternum, and then from the adipose tissue of the gluteal region near the base of the tail. Mononuclear fraction and stromal vascular fraction were isolated from the samples and cultivated in DMEM medium supplemented with 10% fetal bovine serum or in AIM-V medium. The cultures were observed once a week with an inverted microscope, to perform a qualitative analysis of the morphology of the cells as well as the general appearance of the cell culture. Colony-forming units (CFU) were counted on days 5, 15 and 25 of cell culture. During the first week of culture, differences were observed between the samples from the same source maintained in different culture media. The number of colonies was significantly higher in samples of bone marrow in relation to samples of adipose tissue.Colégio Brasileiro de Patologia Animal - CBPA2013-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2013001300004Pesquisa Veterinária Brasileira v.33 suppl.1 2013reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/S0100-736X2013001300004info:eu-repo/semantics/openAccessRibeiro,GesianeMassoco,Cristina O.Lacerda Neto,José Corrêa deeng2014-04-07T00:00:00Zoai:scielo:S0100-736X2013001300004Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2014-04-07T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false
dc.title.none.fl_str_mv Culture of equine bone marrow mononuclear fraction and adipose tissue-derived stromal vascular fraction cells in different media
title Culture of equine bone marrow mononuclear fraction and adipose tissue-derived stromal vascular fraction cells in different media
spellingShingle Culture of equine bone marrow mononuclear fraction and adipose tissue-derived stromal vascular fraction cells in different media
Ribeiro,Gesiane
Horses
bone marrow
adipose tissue
culture medium
title_short Culture of equine bone marrow mononuclear fraction and adipose tissue-derived stromal vascular fraction cells in different media
title_full Culture of equine bone marrow mononuclear fraction and adipose tissue-derived stromal vascular fraction cells in different media
title_fullStr Culture of equine bone marrow mononuclear fraction and adipose tissue-derived stromal vascular fraction cells in different media
title_full_unstemmed Culture of equine bone marrow mononuclear fraction and adipose tissue-derived stromal vascular fraction cells in different media
title_sort Culture of equine bone marrow mononuclear fraction and adipose tissue-derived stromal vascular fraction cells in different media
author Ribeiro,Gesiane
author_facet Ribeiro,Gesiane
Massoco,Cristina O.
Lacerda Neto,José Corrêa de
author_role author
author2 Massoco,Cristina O.
Lacerda Neto,José Corrêa de
author2_role author
author
dc.contributor.author.fl_str_mv Ribeiro,Gesiane
Massoco,Cristina O.
Lacerda Neto,José Corrêa de
dc.subject.por.fl_str_mv Horses
bone marrow
adipose tissue
culture medium
topic Horses
bone marrow
adipose tissue
culture medium
description The objective of this study was to evaluate the culture of equine bone marrow mononuclear fraction and adipose tissue - derived stromal vascular fraction cells in two different cell culture media. Five adult horses were submitted to bone marrow aspiration from the sternum, and then from the adipose tissue of the gluteal region near the base of the tail. Mononuclear fraction and stromal vascular fraction were isolated from the samples and cultivated in DMEM medium supplemented with 10% fetal bovine serum or in AIM-V medium. The cultures were observed once a week with an inverted microscope, to perform a qualitative analysis of the morphology of the cells as well as the general appearance of the cell culture. Colony-forming units (CFU) were counted on days 5, 15 and 25 of cell culture. During the first week of culture, differences were observed between the samples from the same source maintained in different culture media. The number of colonies was significantly higher in samples of bone marrow in relation to samples of adipose tissue.
publishDate 2013
dc.date.none.fl_str_mv 2013-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2013001300004
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2013001300004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-736X2013001300004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
dc.source.none.fl_str_mv Pesquisa Veterinária Brasileira v.33 suppl.1 2013
reponame:Pesquisa Veterinária Brasileira (Online)
instname:Colégio Brasileiro de Patologia Animal (CBPA)
instacron:EMBRAPA
instname_str Colégio Brasileiro de Patologia Animal (CBPA)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Veterinária Brasileira (Online)
collection Pesquisa Veterinária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)
repository.mail.fl_str_mv colegio@cbpa.org.br||pvb@pvb.com.br
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