Culture of equine bone marrow mononuclear fraction and adipose tissue-derived stromal vascular fraction cells in different media

Detalhes bibliográficos
Autor(a) principal: Ribeiro, Gesiane
Data de Publicação: 2013
Outros Autores: Massoco, Cristina O., Lacerda Neto, José Corrêa De [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0100-736X2013001300004
http://hdl.handle.net/11449/109912
Resumo: The objective of this study was to evaluate the culture of equine bone marrow mononuclear fraction and adipose tissue - derived stromal vascular fraction cells in two different cell culture media. Five adult horses were submitted to bone marrow aspiration from the sternum, and then from the adipose tissue of the gluteal region near the base of the tail. Mononuclear fraction and stromal vascular fraction were isolated from the samples and cultivated in DMEM medium supplemented with 10% fetal bovine serum or in AIM-V medium. The cultures were observed once a week with an inverted microscope, to perform a qualitative analysis of the morphology of the cells as well as the general appearance of the cell culture. Colony-forming units (CFU) were counted on days 5, 15 and 25 of cell culture. During the first week of culture, differences were observed between the samples from the same source maintained in different culture media. The number of colonies was significantly higher in samples of bone marrow in relation to samples of adipose tissue.
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spelling Culture of equine bone marrow mononuclear fraction and adipose tissue-derived stromal vascular fraction cells in different mediaCultivo de células da fração mononuclear da medulla óssea e da fração vascular stromal do tecido adipose de equinos em diferentes meiosHorsebone marrowadipose tissueCulture mediumCavalosMedula ósseaTecido adiposomeios de culturaThe objective of this study was to evaluate the culture of equine bone marrow mononuclear fraction and adipose tissue - derived stromal vascular fraction cells in two different cell culture media. Five adult horses were submitted to bone marrow aspiration from the sternum, and then from the adipose tissue of the gluteal region near the base of the tail. Mononuclear fraction and stromal vascular fraction were isolated from the samples and cultivated in DMEM medium supplemented with 10% fetal bovine serum or in AIM-V medium. The cultures were observed once a week with an inverted microscope, to perform a qualitative analysis of the morphology of the cells as well as the general appearance of the cell culture. Colony-forming units (CFU) were counted on days 5, 15 and 25 of cell culture. During the first week of culture, differences were observed between the samples from the same source maintained in different culture media. The number of colonies was significantly higher in samples of bone marrow in relation to samples of adipose tissue.O objetivo deste estudo foi avaliar o cultivo de células da fração mononuclear da medula óssea e da fração vascular estromal do tecido adiposo de equinos em dois diferentes meios. Cinco cavalos foram submetidos à aspiração da medula óssea do esterno e à coleta de tecido adiposo da região glútea, próxima à inserção da cauda. A fração mononuclear e a fração vascular estromal foram obtidas das amostras e cultivadas em meio DMEM suplementado com soro fetal bovino a 10% ou em meio AIM-V. As culturas foram observadas uma vez por semana com um microscópio de luz invertida, com o intuito de se realizar uma análise qualitativa das características morfológicas das células, bem como do aspecto geral do cultivo celular. As unidades formadoras de colônia (CFU) foram contadas nos dias 5, 15 e 25 do cultivo celular. Durante a primeira semana, foram observadas diferenças entre amostras obtidas de mesma origem mantidas em diferentes meios. O número de colônias foi significativamente maior nas amostras de medula óssea em relação às amostras de tecido adiposo.Faculdades Metropolitanas UnidasUniversidade de São Paulo Faculdade de Medicina Veterinária e Zootecnia Departamento de PatologiaUniversidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias Departamento de Clínica e Cirurgia VeterináriaUniversidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias Departamento de Clínica e Cirurgia VeterináriaColégio Brasileiro de Patologia Animal - CBPAFaculdades Metropolitanas UnidasUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Ribeiro, GesianeMassoco, Cristina O.Lacerda Neto, José Corrêa De [UNESP]2014-10-01T13:08:40Z2014-10-01T13:08:40Z2013-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article20-24application/pdfhttp://dx.doi.org/10.1590/S0100-736X2013001300004Pesquisa Veterinária Brasileira. Colégio Brasileiro de Patologia Animal - CBPA, v. 33, p. 20-24, 2013.0100-736Xhttp://hdl.handle.net/11449/10991210.1590/S0100-736X2013001300004S0100-736X2013001300004WOS:000334663500004S0100-736X2013001300004.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPesquisa Veterinária Brasileira0.385info:eu-repo/semantics/openAccess2024-06-06T14:10:22Zoai:repositorio.unesp.br:11449/109912Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:00:42.223118Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Culture of equine bone marrow mononuclear fraction and adipose tissue-derived stromal vascular fraction cells in different media
Cultivo de células da fração mononuclear da medulla óssea e da fração vascular stromal do tecido adipose de equinos em diferentes meios
title Culture of equine bone marrow mononuclear fraction and adipose tissue-derived stromal vascular fraction cells in different media
spellingShingle Culture of equine bone marrow mononuclear fraction and adipose tissue-derived stromal vascular fraction cells in different media
Ribeiro, Gesiane
Horse
bone marrow
adipose tissue
Culture medium
Cavalos
Medula óssea
Tecido adiposo
meios de cultura
title_short Culture of equine bone marrow mononuclear fraction and adipose tissue-derived stromal vascular fraction cells in different media
title_full Culture of equine bone marrow mononuclear fraction and adipose tissue-derived stromal vascular fraction cells in different media
title_fullStr Culture of equine bone marrow mononuclear fraction and adipose tissue-derived stromal vascular fraction cells in different media
title_full_unstemmed Culture of equine bone marrow mononuclear fraction and adipose tissue-derived stromal vascular fraction cells in different media
title_sort Culture of equine bone marrow mononuclear fraction and adipose tissue-derived stromal vascular fraction cells in different media
author Ribeiro, Gesiane
author_facet Ribeiro, Gesiane
Massoco, Cristina O.
Lacerda Neto, José Corrêa De [UNESP]
author_role author
author2 Massoco, Cristina O.
Lacerda Neto, José Corrêa De [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Faculdades Metropolitanas Unidas
Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Ribeiro, Gesiane
Massoco, Cristina O.
Lacerda Neto, José Corrêa De [UNESP]
dc.subject.por.fl_str_mv Horse
bone marrow
adipose tissue
Culture medium
Cavalos
Medula óssea
Tecido adiposo
meios de cultura
topic Horse
bone marrow
adipose tissue
Culture medium
Cavalos
Medula óssea
Tecido adiposo
meios de cultura
description The objective of this study was to evaluate the culture of equine bone marrow mononuclear fraction and adipose tissue - derived stromal vascular fraction cells in two different cell culture media. Five adult horses were submitted to bone marrow aspiration from the sternum, and then from the adipose tissue of the gluteal region near the base of the tail. Mononuclear fraction and stromal vascular fraction were isolated from the samples and cultivated in DMEM medium supplemented with 10% fetal bovine serum or in AIM-V medium. The cultures were observed once a week with an inverted microscope, to perform a qualitative analysis of the morphology of the cells as well as the general appearance of the cell culture. Colony-forming units (CFU) were counted on days 5, 15 and 25 of cell culture. During the first week of culture, differences were observed between the samples from the same source maintained in different culture media. The number of colonies was significantly higher in samples of bone marrow in relation to samples of adipose tissue.
publishDate 2013
dc.date.none.fl_str_mv 2013-12-01
2014-10-01T13:08:40Z
2014-10-01T13:08:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0100-736X2013001300004
Pesquisa Veterinária Brasileira. Colégio Brasileiro de Patologia Animal - CBPA, v. 33, p. 20-24, 2013.
0100-736X
http://hdl.handle.net/11449/109912
10.1590/S0100-736X2013001300004
S0100-736X2013001300004
WOS:000334663500004
S0100-736X2013001300004.pdf
url http://dx.doi.org/10.1590/S0100-736X2013001300004
http://hdl.handle.net/11449/109912
identifier_str_mv Pesquisa Veterinária Brasileira. Colégio Brasileiro de Patologia Animal - CBPA, v. 33, p. 20-24, 2013.
0100-736X
10.1590/S0100-736X2013001300004
S0100-736X2013001300004
WOS:000334663500004
S0100-736X2013001300004.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Pesquisa Veterinária Brasileira
0.385
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 20-24
application/pdf
dc.publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129148831399936

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