Staphylococcal toxin genes in strains isolated from cows with subclinical mastitis
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Veterinária Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2008001200010 |
Resumo: | The present study was carried out in 11 dairy herds in four municipal districts of the rural area of the State of Pernambuco, Brazil. Out of 984 quarter milk (246 cows), 10 (1.0%) were positive for clinical mastitis, 562 (57.1%) for subclinical mastitis and 412 (41.9%) were negative. A total of 81 Staphylococcus spp. isolates were obtained from milk samples from the cows diagnosed with subclinical mastitis. From these, 53 (65.0%) were S. aureus, 16 (20.0%) coagulase-positive staphylococci (CPS) and 12 (15.0%) coagulase-negative staphylococci (CNS). The isolates were further investigated for the presence of toxin genes by multiplex and uniplex PCR. The main gene observed was seg followed by seh, sei and sej. The distribution of these observed genes among the isolates obtained from different areas showed a regional pattern for the SEs. The presence of toxin genes in the strains isolated from bovine milk demonstrates a potential problem for public health. |
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Staphylococcal toxin genes in strains isolated from cows with subclinical mastitisStaphylococcal toxinstoxin genesmilkdairy cowsThe present study was carried out in 11 dairy herds in four municipal districts of the rural area of the State of Pernambuco, Brazil. Out of 984 quarter milk (246 cows), 10 (1.0%) were positive for clinical mastitis, 562 (57.1%) for subclinical mastitis and 412 (41.9%) were negative. A total of 81 Staphylococcus spp. isolates were obtained from milk samples from the cows diagnosed with subclinical mastitis. From these, 53 (65.0%) were S. aureus, 16 (20.0%) coagulase-positive staphylococci (CPS) and 12 (15.0%) coagulase-negative staphylococci (CNS). The isolates were further investigated for the presence of toxin genes by multiplex and uniplex PCR. The main gene observed was seg followed by seh, sei and sej. The distribution of these observed genes among the isolates obtained from different areas showed a regional pattern for the SEs. The presence of toxin genes in the strains isolated from bovine milk demonstrates a potential problem for public health.Colégio Brasileiro de Patologia Animal - CBPA2008-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2008001200010Pesquisa Veterinária Brasileira v.28 n.12 2008reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/S0100-736X2008001200010info:eu-repo/semantics/openAccessFreitas,Manuela F.L. deLuz,Isabelle da S.Silveira-Filho,Vladimir da M.W.P. Júnior,JoséStamford,Tânia L.M.Mota,Rinaldo A.Sena,Maria J. deAlmeida,Alzira M.P. deBalbino,Valdir de Q.Leal-Balbino,Tereza C.eng2009-03-06T00:00:00Zoai:scielo:S0100-736X2008001200010Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2009-03-06T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false |
dc.title.none.fl_str_mv |
Staphylococcal toxin genes in strains isolated from cows with subclinical mastitis |
title |
Staphylococcal toxin genes in strains isolated from cows with subclinical mastitis |
spellingShingle |
Staphylococcal toxin genes in strains isolated from cows with subclinical mastitis Freitas,Manuela F.L. de Staphylococcal toxins toxin genes milk dairy cows |
title_short |
Staphylococcal toxin genes in strains isolated from cows with subclinical mastitis |
title_full |
Staphylococcal toxin genes in strains isolated from cows with subclinical mastitis |
title_fullStr |
Staphylococcal toxin genes in strains isolated from cows with subclinical mastitis |
title_full_unstemmed |
Staphylococcal toxin genes in strains isolated from cows with subclinical mastitis |
title_sort |
Staphylococcal toxin genes in strains isolated from cows with subclinical mastitis |
author |
Freitas,Manuela F.L. de |
author_facet |
Freitas,Manuela F.L. de Luz,Isabelle da S. Silveira-Filho,Vladimir da M. W.P. Júnior,José Stamford,Tânia L.M. Mota,Rinaldo A. Sena,Maria J. de Almeida,Alzira M.P. de Balbino,Valdir de Q. Leal-Balbino,Tereza C. |
author_role |
author |
author2 |
Luz,Isabelle da S. Silveira-Filho,Vladimir da M. W.P. Júnior,José Stamford,Tânia L.M. Mota,Rinaldo A. Sena,Maria J. de Almeida,Alzira M.P. de Balbino,Valdir de Q. Leal-Balbino,Tereza C. |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Freitas,Manuela F.L. de Luz,Isabelle da S. Silveira-Filho,Vladimir da M. W.P. Júnior,José Stamford,Tânia L.M. Mota,Rinaldo A. Sena,Maria J. de Almeida,Alzira M.P. de Balbino,Valdir de Q. Leal-Balbino,Tereza C. |
dc.subject.por.fl_str_mv |
Staphylococcal toxins toxin genes milk dairy cows |
topic |
Staphylococcal toxins toxin genes milk dairy cows |
description |
The present study was carried out in 11 dairy herds in four municipal districts of the rural area of the State of Pernambuco, Brazil. Out of 984 quarter milk (246 cows), 10 (1.0%) were positive for clinical mastitis, 562 (57.1%) for subclinical mastitis and 412 (41.9%) were negative. A total of 81 Staphylococcus spp. isolates were obtained from milk samples from the cows diagnosed with subclinical mastitis. From these, 53 (65.0%) were S. aureus, 16 (20.0%) coagulase-positive staphylococci (CPS) and 12 (15.0%) coagulase-negative staphylococci (CNS). The isolates were further investigated for the presence of toxin genes by multiplex and uniplex PCR. The main gene observed was seg followed by seh, sei and sej. The distribution of these observed genes among the isolates obtained from different areas showed a regional pattern for the SEs. The presence of toxin genes in the strains isolated from bovine milk demonstrates a potential problem for public health. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2008001200010 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2008001200010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-736X2008001200010 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
dc.source.none.fl_str_mv |
Pesquisa Veterinária Brasileira v.28 n.12 2008 reponame:Pesquisa Veterinária Brasileira (Online) instname:Colégio Brasileiro de Patologia Animal (CBPA) instacron:EMBRAPA |
instname_str |
Colégio Brasileiro de Patologia Animal (CBPA) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Veterinária Brasileira (Online) |
collection |
Pesquisa Veterinária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA) |
repository.mail.fl_str_mv |
colegio@cbpa.org.br||pvb@pvb.com.br |
_version_ |
1754122229161918464 |