Use of photodynamic therapy in the treatment of bovine subclinical mastitis

Detalhes bibliográficos
Autor(a) principal: Moreira, Lívia Helena
Data de Publicação: 2018
Outros Autores: de Souza, José Carlos Pereira, de Lima, Carlos José, Salgado, Miguel Angel Castillo [UNESP], Fernandes, Adriana Barrinha, Andreani, Dora Inés Kozusny, Villaverde, Antonio Balbin, Zângaro, Renato Amaro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.pdpdt.2017.12.009
http://hdl.handle.net/11449/179502
Resumo: Background Bovine mastitis is a disease that causes a severe drawback in dairy production. Conventional treatments with antibiotic could leave antibiotic residues in the milk. The aim of this study was to evaluate the effect of photodynamic therapy in the treatment of bovine subclinical mastitis to develop an in vivo therapeutic protocol that could be used in routine farm practice, favoring the early return to production. Methods Forty cows with subclinical mastitis (n = 40) were divided into 4 groups (control, photodynamic therapy – PDT, light irradiation – LED, and photosensitizer – PS). Control group received no treatment, PDT group received application of 1.0 mL of 2.5% toluidine blue photosensitizer followed by LED irradiation at λ = 635 nm, the LED group was treated with LED irradiation alone, and the PS group received only 2.5% toluidine blue dye. LED irradiation was applied to the mammary gland by means of an acrylic light guide coupled to the LED equipment. The PDT and LED groups were irradiated with 200 J/cm2 at three different positions inside the mammary gland. Milk samples were collected at 0 h, 12 h, 24 h after treatment for microbial identification and total bacterial count. Results The treatment of the PDT group showed significant difference p < 0.05, characterizing the efficiency of this technique with the reduction of the microorganisms Streptococcus dysgalactiae and coagulase-negative Staphylococcus. Conclusion Photodynamic therapy was effective when applied in vivo for subclinical bovine mastitis. There was no need to separate the animal from production.
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spelling Use of photodynamic therapy in the treatment of bovine subclinical mastitisDairy cowsDisease of cowsPhotosensitizerPhototherapyRed LEDVeterinary medicineBackground Bovine mastitis is a disease that causes a severe drawback in dairy production. Conventional treatments with antibiotic could leave antibiotic residues in the milk. The aim of this study was to evaluate the effect of photodynamic therapy in the treatment of bovine subclinical mastitis to develop an in vivo therapeutic protocol that could be used in routine farm practice, favoring the early return to production. Methods Forty cows with subclinical mastitis (n = 40) were divided into 4 groups (control, photodynamic therapy – PDT, light irradiation – LED, and photosensitizer – PS). Control group received no treatment, PDT group received application of 1.0 mL of 2.5% toluidine blue photosensitizer followed by LED irradiation at λ = 635 nm, the LED group was treated with LED irradiation alone, and the PS group received only 2.5% toluidine blue dye. LED irradiation was applied to the mammary gland by means of an acrylic light guide coupled to the LED equipment. The PDT and LED groups were irradiated with 200 J/cm2 at three different positions inside the mammary gland. Milk samples were collected at 0 h, 12 h, 24 h after treatment for microbial identification and total bacterial count. Results The treatment of the PDT group showed significant difference p < 0.05, characterizing the efficiency of this technique with the reduction of the microorganisms Streptococcus dysgalactiae and coagulase-negative Staphylococcus. Conclusion Photodynamic therapy was effective when applied in vivo for subclinical bovine mastitis. There was no need to separate the animal from production.Universidade Anhembi Morumbi (UAM), Rua Casa do Ator, 294, Campus Vila OlímpiaCentro de Inovação Tecnologia e Educação (CITÉ), Estrada Dr. Altino Bondesan, 500, Distrito de Eugênio de MeloMinistério da Agricultura Pecuária e Abastecimento (MAPA), Av. Rodrigues Alves 129, 7° andar, Praça. Mauá CentroUniversidade do Estado de São Paulo (UNESP), Avenida José Longo, 777, Jardim São DimasUniversidade Brasil (UNIBRASIL), Estrada Projetada F-1, s/n − Fazenda Santa RitaUniversidade do Estado de São Paulo (UNESP), Avenida José Longo, 777, Jardim São DimasUniversidade Anhembi Morumbi (UAM)Tecnologia e Educação (CITÉ)Pecuária e Abastecimento (MAPA)Universidade Estadual Paulista (Unesp)Universidade Brasil (UNIBRASIL)Moreira, Lívia Helenade Souza, José Carlos Pereirade Lima, Carlos JoséSalgado, Miguel Angel Castillo [UNESP]Fernandes, Adriana BarrinhaAndreani, Dora Inés KozusnyVillaverde, Antonio BalbinZângaro, Renato Amaro2018-12-11T17:35:26Z2018-12-11T17:35:26Z2018-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article246-251application/pdfhttp://dx.doi.org/10.1016/j.pdpdt.2017.12.009Photodiagnosis and Photodynamic Therapy, v. 21, p. 246-251.1873-15971572-1000http://hdl.handle.net/11449/17950210.1016/j.pdpdt.2017.12.0092-s2.0-850404493622-s2.0-85040449362.pdf8235700830441386Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhotodiagnosis and Photodynamic Therapy0,647info:eu-repo/semantics/openAccess2023-10-17T06:09:06Zoai:repositorio.unesp.br:11449/179502Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:11:41.275407Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Use of photodynamic therapy in the treatment of bovine subclinical mastitis
title Use of photodynamic therapy in the treatment of bovine subclinical mastitis
spellingShingle Use of photodynamic therapy in the treatment of bovine subclinical mastitis
Moreira, Lívia Helena
Dairy cows
Disease of cows
Photosensitizer
Phototherapy
Red LED
Veterinary medicine
title_short Use of photodynamic therapy in the treatment of bovine subclinical mastitis
title_full Use of photodynamic therapy in the treatment of bovine subclinical mastitis
title_fullStr Use of photodynamic therapy in the treatment of bovine subclinical mastitis
title_full_unstemmed Use of photodynamic therapy in the treatment of bovine subclinical mastitis
title_sort Use of photodynamic therapy in the treatment of bovine subclinical mastitis
author Moreira, Lívia Helena
author_facet Moreira, Lívia Helena
de Souza, José Carlos Pereira
de Lima, Carlos José
Salgado, Miguel Angel Castillo [UNESP]
Fernandes, Adriana Barrinha
Andreani, Dora Inés Kozusny
Villaverde, Antonio Balbin
Zângaro, Renato Amaro
author_role author
author2 de Souza, José Carlos Pereira
de Lima, Carlos José
Salgado, Miguel Angel Castillo [UNESP]
Fernandes, Adriana Barrinha
Andreani, Dora Inés Kozusny
Villaverde, Antonio Balbin
Zângaro, Renato Amaro
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Anhembi Morumbi (UAM)
Tecnologia e Educação (CITÉ)
Pecuária e Abastecimento (MAPA)
Universidade Estadual Paulista (Unesp)
Universidade Brasil (UNIBRASIL)
dc.contributor.author.fl_str_mv Moreira, Lívia Helena
de Souza, José Carlos Pereira
de Lima, Carlos José
Salgado, Miguel Angel Castillo [UNESP]
Fernandes, Adriana Barrinha
Andreani, Dora Inés Kozusny
Villaverde, Antonio Balbin
Zângaro, Renato Amaro
dc.subject.por.fl_str_mv Dairy cows
Disease of cows
Photosensitizer
Phototherapy
Red LED
Veterinary medicine
topic Dairy cows
Disease of cows
Photosensitizer
Phototherapy
Red LED
Veterinary medicine
description Background Bovine mastitis is a disease that causes a severe drawback in dairy production. Conventional treatments with antibiotic could leave antibiotic residues in the milk. The aim of this study was to evaluate the effect of photodynamic therapy in the treatment of bovine subclinical mastitis to develop an in vivo therapeutic protocol that could be used in routine farm practice, favoring the early return to production. Methods Forty cows with subclinical mastitis (n = 40) were divided into 4 groups (control, photodynamic therapy – PDT, light irradiation – LED, and photosensitizer – PS). Control group received no treatment, PDT group received application of 1.0 mL of 2.5% toluidine blue photosensitizer followed by LED irradiation at λ = 635 nm, the LED group was treated with LED irradiation alone, and the PS group received only 2.5% toluidine blue dye. LED irradiation was applied to the mammary gland by means of an acrylic light guide coupled to the LED equipment. The PDT and LED groups were irradiated with 200 J/cm2 at three different positions inside the mammary gland. Milk samples were collected at 0 h, 12 h, 24 h after treatment for microbial identification and total bacterial count. Results The treatment of the PDT group showed significant difference p < 0.05, characterizing the efficiency of this technique with the reduction of the microorganisms Streptococcus dysgalactiae and coagulase-negative Staphylococcus. Conclusion Photodynamic therapy was effective when applied in vivo for subclinical bovine mastitis. There was no need to separate the animal from production.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T17:35:26Z
2018-12-11T17:35:26Z
2018-03-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.pdpdt.2017.12.009
Photodiagnosis and Photodynamic Therapy, v. 21, p. 246-251.
1873-1597
1572-1000
http://hdl.handle.net/11449/179502
10.1016/j.pdpdt.2017.12.009
2-s2.0-85040449362
2-s2.0-85040449362.pdf
8235700830441386
url http://dx.doi.org/10.1016/j.pdpdt.2017.12.009
http://hdl.handle.net/11449/179502
identifier_str_mv Photodiagnosis and Photodynamic Therapy, v. 21, p. 246-251.
1873-1597
1572-1000
10.1016/j.pdpdt.2017.12.009
2-s2.0-85040449362
2-s2.0-85040449362.pdf
8235700830441386
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Photodiagnosis and Photodynamic Therapy
0,647
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 246-251
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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