Use of photodynamic therapy in the treatment of bovine subclinical mastitis
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.pdpdt.2017.12.009 http://hdl.handle.net/11449/179502 |
Resumo: | Background Bovine mastitis is a disease that causes a severe drawback in dairy production. Conventional treatments with antibiotic could leave antibiotic residues in the milk. The aim of this study was to evaluate the effect of photodynamic therapy in the treatment of bovine subclinical mastitis to develop an in vivo therapeutic protocol that could be used in routine farm practice, favoring the early return to production. Methods Forty cows with subclinical mastitis (n = 40) were divided into 4 groups (control, photodynamic therapy – PDT, light irradiation – LED, and photosensitizer – PS). Control group received no treatment, PDT group received application of 1.0 mL of 2.5% toluidine blue photosensitizer followed by LED irradiation at λ = 635 nm, the LED group was treated with LED irradiation alone, and the PS group received only 2.5% toluidine blue dye. LED irradiation was applied to the mammary gland by means of an acrylic light guide coupled to the LED equipment. The PDT and LED groups were irradiated with 200 J/cm2 at three different positions inside the mammary gland. Milk samples were collected at 0 h, 12 h, 24 h after treatment for microbial identification and total bacterial count. Results The treatment of the PDT group showed significant difference p < 0.05, characterizing the efficiency of this technique with the reduction of the microorganisms Streptococcus dysgalactiae and coagulase-negative Staphylococcus. Conclusion Photodynamic therapy was effective when applied in vivo for subclinical bovine mastitis. There was no need to separate the animal from production. |
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Use of photodynamic therapy in the treatment of bovine subclinical mastitisDairy cowsDisease of cowsPhotosensitizerPhototherapyRed LEDVeterinary medicineBackground Bovine mastitis is a disease that causes a severe drawback in dairy production. Conventional treatments with antibiotic could leave antibiotic residues in the milk. The aim of this study was to evaluate the effect of photodynamic therapy in the treatment of bovine subclinical mastitis to develop an in vivo therapeutic protocol that could be used in routine farm practice, favoring the early return to production. Methods Forty cows with subclinical mastitis (n = 40) were divided into 4 groups (control, photodynamic therapy – PDT, light irradiation – LED, and photosensitizer – PS). Control group received no treatment, PDT group received application of 1.0 mL of 2.5% toluidine blue photosensitizer followed by LED irradiation at λ = 635 nm, the LED group was treated with LED irradiation alone, and the PS group received only 2.5% toluidine blue dye. LED irradiation was applied to the mammary gland by means of an acrylic light guide coupled to the LED equipment. The PDT and LED groups were irradiated with 200 J/cm2 at three different positions inside the mammary gland. Milk samples were collected at 0 h, 12 h, 24 h after treatment for microbial identification and total bacterial count. Results The treatment of the PDT group showed significant difference p < 0.05, characterizing the efficiency of this technique with the reduction of the microorganisms Streptococcus dysgalactiae and coagulase-negative Staphylococcus. Conclusion Photodynamic therapy was effective when applied in vivo for subclinical bovine mastitis. There was no need to separate the animal from production.Universidade Anhembi Morumbi (UAM), Rua Casa do Ator, 294, Campus Vila OlímpiaCentro de Inovação Tecnologia e Educação (CITÉ), Estrada Dr. Altino Bondesan, 500, Distrito de Eugênio de MeloMinistério da Agricultura Pecuária e Abastecimento (MAPA), Av. Rodrigues Alves 129, 7° andar, Praça. Mauá CentroUniversidade do Estado de São Paulo (UNESP), Avenida José Longo, 777, Jardim São DimasUniversidade Brasil (UNIBRASIL), Estrada Projetada F-1, s/n − Fazenda Santa RitaUniversidade do Estado de São Paulo (UNESP), Avenida José Longo, 777, Jardim São DimasUniversidade Anhembi Morumbi (UAM)Tecnologia e Educação (CITÉ)Pecuária e Abastecimento (MAPA)Universidade Estadual Paulista (Unesp)Universidade Brasil (UNIBRASIL)Moreira, Lívia Helenade Souza, José Carlos Pereirade Lima, Carlos JoséSalgado, Miguel Angel Castillo [UNESP]Fernandes, Adriana BarrinhaAndreani, Dora Inés KozusnyVillaverde, Antonio BalbinZângaro, Renato Amaro2018-12-11T17:35:26Z2018-12-11T17:35:26Z2018-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article246-251application/pdfhttp://dx.doi.org/10.1016/j.pdpdt.2017.12.009Photodiagnosis and Photodynamic Therapy, v. 21, p. 246-251.1873-15971572-1000http://hdl.handle.net/11449/17950210.1016/j.pdpdt.2017.12.0092-s2.0-850404493622-s2.0-85040449362.pdf8235700830441386Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhotodiagnosis and Photodynamic Therapy0,647info:eu-repo/semantics/openAccess2023-10-17T06:09:06Zoai:repositorio.unesp.br:11449/179502Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:11:41.275407Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Use of photodynamic therapy in the treatment of bovine subclinical mastitis |
title |
Use of photodynamic therapy in the treatment of bovine subclinical mastitis |
spellingShingle |
Use of photodynamic therapy in the treatment of bovine subclinical mastitis Moreira, Lívia Helena Dairy cows Disease of cows Photosensitizer Phototherapy Red LED Veterinary medicine |
title_short |
Use of photodynamic therapy in the treatment of bovine subclinical mastitis |
title_full |
Use of photodynamic therapy in the treatment of bovine subclinical mastitis |
title_fullStr |
Use of photodynamic therapy in the treatment of bovine subclinical mastitis |
title_full_unstemmed |
Use of photodynamic therapy in the treatment of bovine subclinical mastitis |
title_sort |
Use of photodynamic therapy in the treatment of bovine subclinical mastitis |
author |
Moreira, Lívia Helena |
author_facet |
Moreira, Lívia Helena de Souza, José Carlos Pereira de Lima, Carlos José Salgado, Miguel Angel Castillo [UNESP] Fernandes, Adriana Barrinha Andreani, Dora Inés Kozusny Villaverde, Antonio Balbin Zângaro, Renato Amaro |
author_role |
author |
author2 |
de Souza, José Carlos Pereira de Lima, Carlos José Salgado, Miguel Angel Castillo [UNESP] Fernandes, Adriana Barrinha Andreani, Dora Inés Kozusny Villaverde, Antonio Balbin Zângaro, Renato Amaro |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Anhembi Morumbi (UAM) Tecnologia e Educação (CITÉ) Pecuária e Abastecimento (MAPA) Universidade Estadual Paulista (Unesp) Universidade Brasil (UNIBRASIL) |
dc.contributor.author.fl_str_mv |
Moreira, Lívia Helena de Souza, José Carlos Pereira de Lima, Carlos José Salgado, Miguel Angel Castillo [UNESP] Fernandes, Adriana Barrinha Andreani, Dora Inés Kozusny Villaverde, Antonio Balbin Zângaro, Renato Amaro |
dc.subject.por.fl_str_mv |
Dairy cows Disease of cows Photosensitizer Phototherapy Red LED Veterinary medicine |
topic |
Dairy cows Disease of cows Photosensitizer Phototherapy Red LED Veterinary medicine |
description |
Background Bovine mastitis is a disease that causes a severe drawback in dairy production. Conventional treatments with antibiotic could leave antibiotic residues in the milk. The aim of this study was to evaluate the effect of photodynamic therapy in the treatment of bovine subclinical mastitis to develop an in vivo therapeutic protocol that could be used in routine farm practice, favoring the early return to production. Methods Forty cows with subclinical mastitis (n = 40) were divided into 4 groups (control, photodynamic therapy – PDT, light irradiation – LED, and photosensitizer – PS). Control group received no treatment, PDT group received application of 1.0 mL of 2.5% toluidine blue photosensitizer followed by LED irradiation at λ = 635 nm, the LED group was treated with LED irradiation alone, and the PS group received only 2.5% toluidine blue dye. LED irradiation was applied to the mammary gland by means of an acrylic light guide coupled to the LED equipment. The PDT and LED groups were irradiated with 200 J/cm2 at three different positions inside the mammary gland. Milk samples were collected at 0 h, 12 h, 24 h after treatment for microbial identification and total bacterial count. Results The treatment of the PDT group showed significant difference p < 0.05, characterizing the efficiency of this technique with the reduction of the microorganisms Streptococcus dysgalactiae and coagulase-negative Staphylococcus. Conclusion Photodynamic therapy was effective when applied in vivo for subclinical bovine mastitis. There was no need to separate the animal from production. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:35:26Z 2018-12-11T17:35:26Z 2018-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.pdpdt.2017.12.009 Photodiagnosis and Photodynamic Therapy, v. 21, p. 246-251. 1873-1597 1572-1000 http://hdl.handle.net/11449/179502 10.1016/j.pdpdt.2017.12.009 2-s2.0-85040449362 2-s2.0-85040449362.pdf 8235700830441386 |
url |
http://dx.doi.org/10.1016/j.pdpdt.2017.12.009 http://hdl.handle.net/11449/179502 |
identifier_str_mv |
Photodiagnosis and Photodynamic Therapy, v. 21, p. 246-251. 1873-1597 1572-1000 10.1016/j.pdpdt.2017.12.009 2-s2.0-85040449362 2-s2.0-85040449362.pdf 8235700830441386 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Photodiagnosis and Photodynamic Therapy 0,647 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
246-251 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128477868589056 |