Genetic variability of Brazilian phytoplasma and spiroplasma isolated from maize plants

Detalhes bibliográficos
Autor(a) principal: Gomes, Eliane Aparecida
Data de Publicação: 2004
Outros Autores: Jardim, Sílvia Neto, Guimarães, Claudia Teixeira, de Souza, Isabel Regina Prazeres, de Oliveira, Elizabeth
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/6737
Resumo: The objective of this work was to characterize the genetic variability of phytoplasma and Spiroplasma kunkelii isolated from maize plants showing symptoms of stunt collected from different Brazilian geographic regions. A DNA fragment of 500 base pairs (bp) was amplified from the spiralin gene in S. kunkelii and one fragment of 1,200 bp was generated from 16S rDNA gene in phytoplasma. The partial sequences of the spiralin gene showed similarity of 98% among the isolates of S. kunkelii analyzed. These sequences were compared with the sequence of the spiralin gene from other Spiroplasma species deposited in the GenBank, resulting in a similarity varying from 76.9% to 88.1%. The 16S rDNA sequence from the phytoplasma were completely similar within the Brazilian isolates and showed up to 98% of the similarity with sequences already found from other phytoplasmas. A very narrow genetic variability was detected by these gene fragments within phytoplasma and Spiroplasma analyzed. However, other genomic regions with higher polymorphic levels shall be identified in order to better evaluate the genetic diversity within these microorganisms population.
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spelling Genetic variability of Brazilian phytoplasma and spiroplasma isolated from maize plantsVariabilidade genética de fitoplasma e espiroplasma isolados de plantas de milho no BrasilZea mays; microorganisms; spiralin; mollicutes; genetic polymorphism; gene sequenceZea mays; microrganismo; espiralina; molicute; polimorfismo genético; seqüência genéticaThe objective of this work was to characterize the genetic variability of phytoplasma and Spiroplasma kunkelii isolated from maize plants showing symptoms of stunt collected from different Brazilian geographic regions. A DNA fragment of 500 base pairs (bp) was amplified from the spiralin gene in S. kunkelii and one fragment of 1,200 bp was generated from 16S rDNA gene in phytoplasma. The partial sequences of the spiralin gene showed similarity of 98% among the isolates of S. kunkelii analyzed. These sequences were compared with the sequence of the spiralin gene from other Spiroplasma species deposited in the GenBank, resulting in a similarity varying from 76.9% to 88.1%. The 16S rDNA sequence from the phytoplasma were completely similar within the Brazilian isolates and showed up to 98% of the similarity with sequences already found from other phytoplasmas. A very narrow genetic variability was detected by these gene fragments within phytoplasma and Spiroplasma analyzed. However, other genomic regions with higher polymorphic levels shall be identified in order to better evaluate the genetic diversity within these microorganisms population.O objetivo deste trabalho foi caracterizar a variabilidade genética de isolados de fitoplasma e de Spiroplasma kunkelii obtidos de plantas de milho, apresentando sintomas de enfezamento, coletados em diferentes regiões do Brasil. Um fragmento de 500 pares de bases (pb) do gene que codifica a espiralina de S. kunkelii foi amplificado e um produto de amplificação de 1.200 pb foi gerado a partir do gene 16S rDNA de fitoplasma. As seqüências parciais do gene da espiralina mostraram similaridade de 98% entre os isolados de S. kunkelii analisados. Essas seqüências foram comparadas com a seqüência do gene da espiralina de outras espécies de Spiroplasma depositadas no GenBank, resultando em similaridade variável entre 76,9% e 88,1%. As seqüências do gene 16S rDNA dos isolados de fitoplasma foram completamente similares entre todos os isolados brasileiros e apresentaram até 98% de similaridade com seqüências do mesmo gene de outros fitoplasmas já publicadas. Uma variabilidade genética muito estreita foi detectada para esses genes entre os isolados de fitoplasma e Spiroplasma analisados. No entanto, outras regiões genômicas, que apresentem um maior polimorfismo precisam ser identificadas para melhor avaliar a diversidade genética dentro da população desses microrganismos.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraGomes, Eliane AparecidaJardim, Sílvia NetoGuimarães, Claudia Teixeirade Souza, Isabel Regina Prazeresde Oliveira, Elizabeth2004-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/6737Pesquisa Agropecuaria Brasileira; v.39, n.1, jan. 2004; 61-65Pesquisa Agropecuária Brasileira; v.39, n.1, jan. 2004; 61-651678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/6737/3793info:eu-repo/semantics/openAccess2014-07-14T18:08:37Zoai:ojs.seer.sct.embrapa.br:article/6737Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-07-14T18:08:37Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Genetic variability of Brazilian phytoplasma and spiroplasma isolated from maize plants
Variabilidade genética de fitoplasma e espiroplasma isolados de plantas de milho no Brasil
title Genetic variability of Brazilian phytoplasma and spiroplasma isolated from maize plants
spellingShingle Genetic variability of Brazilian phytoplasma and spiroplasma isolated from maize plants
Gomes, Eliane Aparecida
Zea mays; microorganisms; spiralin; mollicutes; genetic polymorphism; gene sequence
Zea mays; microrganismo; espiralina; molicute; polimorfismo genético; seqüência genética
title_short Genetic variability of Brazilian phytoplasma and spiroplasma isolated from maize plants
title_full Genetic variability of Brazilian phytoplasma and spiroplasma isolated from maize plants
title_fullStr Genetic variability of Brazilian phytoplasma and spiroplasma isolated from maize plants
title_full_unstemmed Genetic variability of Brazilian phytoplasma and spiroplasma isolated from maize plants
title_sort Genetic variability of Brazilian phytoplasma and spiroplasma isolated from maize plants
author Gomes, Eliane Aparecida
author_facet Gomes, Eliane Aparecida
Jardim, Sílvia Neto
Guimarães, Claudia Teixeira
de Souza, Isabel Regina Prazeres
de Oliveira, Elizabeth
author_role author
author2 Jardim, Sílvia Neto
Guimarães, Claudia Teixeira
de Souza, Isabel Regina Prazeres
de Oliveira, Elizabeth
author2_role author
author
author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Gomes, Eliane Aparecida
Jardim, Sílvia Neto
Guimarães, Claudia Teixeira
de Souza, Isabel Regina Prazeres
de Oliveira, Elizabeth
dc.subject.por.fl_str_mv Zea mays; microorganisms; spiralin; mollicutes; genetic polymorphism; gene sequence
Zea mays; microrganismo; espiralina; molicute; polimorfismo genético; seqüência genética
topic Zea mays; microorganisms; spiralin; mollicutes; genetic polymorphism; gene sequence
Zea mays; microrganismo; espiralina; molicute; polimorfismo genético; seqüência genética
description The objective of this work was to characterize the genetic variability of phytoplasma and Spiroplasma kunkelii isolated from maize plants showing symptoms of stunt collected from different Brazilian geographic regions. A DNA fragment of 500 base pairs (bp) was amplified from the spiralin gene in S. kunkelii and one fragment of 1,200 bp was generated from 16S rDNA gene in phytoplasma. The partial sequences of the spiralin gene showed similarity of 98% among the isolates of S. kunkelii analyzed. These sequences were compared with the sequence of the spiralin gene from other Spiroplasma species deposited in the GenBank, resulting in a similarity varying from 76.9% to 88.1%. The 16S rDNA sequence from the phytoplasma were completely similar within the Brazilian isolates and showed up to 98% of the similarity with sequences already found from other phytoplasmas. A very narrow genetic variability was detected by these gene fragments within phytoplasma and Spiroplasma analyzed. However, other genomic regions with higher polymorphic levels shall be identified in order to better evaluate the genetic diversity within these microorganisms population.
publishDate 2004
dc.date.none.fl_str_mv 2004-01-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/6737
url https://seer.sct.embrapa.br/index.php/pab/article/view/6737
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/6737/3793
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.39, n.1, jan. 2004; 61-65
Pesquisa Agropecuária Brasileira; v.39, n.1, jan. 2004; 61-65
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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