Genetic variability of Brazilian phytoplasma and spiroplasma isolated from maize plants
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Agropecuária Brasileira (Online) |
Texto Completo: | https://seer.sct.embrapa.br/index.php/pab/article/view/6737 |
Resumo: | The objective of this work was to characterize the genetic variability of phytoplasma and Spiroplasma kunkelii isolated from maize plants showing symptoms of stunt collected from different Brazilian geographic regions. A DNA fragment of 500 base pairs (bp) was amplified from the spiralin gene in S. kunkelii and one fragment of 1,200 bp was generated from 16S rDNA gene in phytoplasma. The partial sequences of the spiralin gene showed similarity of 98% among the isolates of S. kunkelii analyzed. These sequences were compared with the sequence of the spiralin gene from other Spiroplasma species deposited in the GenBank, resulting in a similarity varying from 76.9% to 88.1%. The 16S rDNA sequence from the phytoplasma were completely similar within the Brazilian isolates and showed up to 98% of the similarity with sequences already found from other phytoplasmas. A very narrow genetic variability was detected by these gene fragments within phytoplasma and Spiroplasma analyzed. However, other genomic regions with higher polymorphic levels shall be identified in order to better evaluate the genetic diversity within these microorganisms population. |
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Genetic variability of Brazilian phytoplasma and spiroplasma isolated from maize plantsVariabilidade genética de fitoplasma e espiroplasma isolados de plantas de milho no BrasilZea mays; microorganisms; spiralin; mollicutes; genetic polymorphism; gene sequenceZea mays; microrganismo; espiralina; molicute; polimorfismo genético; seqüência genéticaThe objective of this work was to characterize the genetic variability of phytoplasma and Spiroplasma kunkelii isolated from maize plants showing symptoms of stunt collected from different Brazilian geographic regions. A DNA fragment of 500 base pairs (bp) was amplified from the spiralin gene in S. kunkelii and one fragment of 1,200 bp was generated from 16S rDNA gene in phytoplasma. The partial sequences of the spiralin gene showed similarity of 98% among the isolates of S. kunkelii analyzed. These sequences were compared with the sequence of the spiralin gene from other Spiroplasma species deposited in the GenBank, resulting in a similarity varying from 76.9% to 88.1%. The 16S rDNA sequence from the phytoplasma were completely similar within the Brazilian isolates and showed up to 98% of the similarity with sequences already found from other phytoplasmas. A very narrow genetic variability was detected by these gene fragments within phytoplasma and Spiroplasma analyzed. However, other genomic regions with higher polymorphic levels shall be identified in order to better evaluate the genetic diversity within these microorganisms population.O objetivo deste trabalho foi caracterizar a variabilidade genética de isolados de fitoplasma e de Spiroplasma kunkelii obtidos de plantas de milho, apresentando sintomas de enfezamento, coletados em diferentes regiões do Brasil. Um fragmento de 500 pares de bases (pb) do gene que codifica a espiralina de S. kunkelii foi amplificado e um produto de amplificação de 1.200 pb foi gerado a partir do gene 16S rDNA de fitoplasma. As seqüências parciais do gene da espiralina mostraram similaridade de 98% entre os isolados de S. kunkelii analisados. Essas seqüências foram comparadas com a seqüência do gene da espiralina de outras espécies de Spiroplasma depositadas no GenBank, resultando em similaridade variável entre 76,9% e 88,1%. As seqüências do gene 16S rDNA dos isolados de fitoplasma foram completamente similares entre todos os isolados brasileiros e apresentaram até 98% de similaridade com seqüências do mesmo gene de outros fitoplasmas já publicadas. Uma variabilidade genética muito estreita foi detectada para esses genes entre os isolados de fitoplasma e Spiroplasma analisados. No entanto, outras regiões genômicas, que apresentem um maior polimorfismo precisam ser identificadas para melhor avaliar a diversidade genética dentro da população desses microrganismos.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraGomes, Eliane AparecidaJardim, Sílvia NetoGuimarães, Claudia Teixeirade Souza, Isabel Regina Prazeresde Oliveira, Elizabeth2004-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/6737Pesquisa Agropecuaria Brasileira; v.39, n.1, jan. 2004; 61-65Pesquisa Agropecuária Brasileira; v.39, n.1, jan. 2004; 61-651678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/6737/3793info:eu-repo/semantics/openAccess2014-07-14T18:08:37Zoai:ojs.seer.sct.embrapa.br:article/6737Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-07-14T18:08:37Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Genetic variability of Brazilian phytoplasma and spiroplasma isolated from maize plants Variabilidade genética de fitoplasma e espiroplasma isolados de plantas de milho no Brasil |
title |
Genetic variability of Brazilian phytoplasma and spiroplasma isolated from maize plants |
spellingShingle |
Genetic variability of Brazilian phytoplasma and spiroplasma isolated from maize plants Gomes, Eliane Aparecida Zea mays; microorganisms; spiralin; mollicutes; genetic polymorphism; gene sequence Zea mays; microrganismo; espiralina; molicute; polimorfismo genético; seqüência genética |
title_short |
Genetic variability of Brazilian phytoplasma and spiroplasma isolated from maize plants |
title_full |
Genetic variability of Brazilian phytoplasma and spiroplasma isolated from maize plants |
title_fullStr |
Genetic variability of Brazilian phytoplasma and spiroplasma isolated from maize plants |
title_full_unstemmed |
Genetic variability of Brazilian phytoplasma and spiroplasma isolated from maize plants |
title_sort |
Genetic variability of Brazilian phytoplasma and spiroplasma isolated from maize plants |
author |
Gomes, Eliane Aparecida |
author_facet |
Gomes, Eliane Aparecida Jardim, Sílvia Neto Guimarães, Claudia Teixeira de Souza, Isabel Regina Prazeres de Oliveira, Elizabeth |
author_role |
author |
author2 |
Jardim, Sílvia Neto Guimarães, Claudia Teixeira de Souza, Isabel Regina Prazeres de Oliveira, Elizabeth |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
|
dc.contributor.author.fl_str_mv |
Gomes, Eliane Aparecida Jardim, Sílvia Neto Guimarães, Claudia Teixeira de Souza, Isabel Regina Prazeres de Oliveira, Elizabeth |
dc.subject.por.fl_str_mv |
Zea mays; microorganisms; spiralin; mollicutes; genetic polymorphism; gene sequence Zea mays; microrganismo; espiralina; molicute; polimorfismo genético; seqüência genética |
topic |
Zea mays; microorganisms; spiralin; mollicutes; genetic polymorphism; gene sequence Zea mays; microrganismo; espiralina; molicute; polimorfismo genético; seqüência genética |
description |
The objective of this work was to characterize the genetic variability of phytoplasma and Spiroplasma kunkelii isolated from maize plants showing symptoms of stunt collected from different Brazilian geographic regions. A DNA fragment of 500 base pairs (bp) was amplified from the spiralin gene in S. kunkelii and one fragment of 1,200 bp was generated from 16S rDNA gene in phytoplasma. The partial sequences of the spiralin gene showed similarity of 98% among the isolates of S. kunkelii analyzed. These sequences were compared with the sequence of the spiralin gene from other Spiroplasma species deposited in the GenBank, resulting in a similarity varying from 76.9% to 88.1%. The 16S rDNA sequence from the phytoplasma were completely similar within the Brazilian isolates and showed up to 98% of the similarity with sequences already found from other phytoplasmas. A very narrow genetic variability was detected by these gene fragments within phytoplasma and Spiroplasma analyzed. However, other genomic regions with higher polymorphic levels shall be identified in order to better evaluate the genetic diversity within these microorganisms population. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-01-01 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/6737 |
url |
https://seer.sct.embrapa.br/index.php/pab/article/view/6737 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/6737/3793 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
dc.source.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira; v.39, n.1, jan. 2004; 61-65 Pesquisa Agropecuária Brasileira; v.39, n.1, jan. 2004; 61-65 1678-3921 0100-104x reponame:Pesquisa Agropecuária Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Agropecuária Brasileira (Online) |
collection |
Pesquisa Agropecuária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
pab@sct.embrapa.br || sct.pab@embrapa.br |
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1793416654448754688 |