Characterization and genetic diversity of elephant grass cultivars and their hybrids with millet through isoenzymatic patterns

Detalhes bibliográficos
Autor(a) principal: Freitas, Nara Suzi Aguiar de
Data de Publicação: 2000
Outros Autores: Falcão, Tânia Maria Muniz de Arruda, Burity, Hélio Almeida, Tabosa, José Nildo, Silva, Márcia Vânusa da
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/5901
Resumo: The isoenzymatic patterns of seven cultivars of elephant grass (Pennisetum purpureum) and their seven hybrids with Pearl millet (P. americanum), selected by Empresa Pernambucana de Pesquisa Agropecuária (IPA), were evaluated to identify the accesses. In polyacrylamide gels, the systems peroxidase (POX), esterase (EST), glutamate oxaloacetate transaminase (GOT), leucine amino peptidase (LAP), alcohol dehydrogenase (ADH), and acid phosphatase (ACP), were studied in young leaves at 28 days after uniformization cut. It wasn't observed isoenzymatic activity of ADH and the efficiency of the LAP system was considered low, which suggest that both can not be indicated for germoplasm characterization. The patterns ACP, GOT, POX and EST allowed the identification of the 14 accesses studied, which revealed 9, 3, 13 and 19 different electrophoretic patterns respectively. Using the esterase system alone, it is possible to identify the germoplasm bank of elephant grass quickly and accurately.
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spelling Characterization and genetic diversity of elephant grass cultivars and their hybrids with millet through isoenzymatic patternsCaracterização e diversidade genética do capim-elefante e seus híbridos com milheto mediante padrões isoenzimáticosPennisetum purpureum; Pennisetum americanum; gramineae; isoenzymes; genetic variationPennisetum purpureum; Pennisetum americanum; gramíneas; isoenzimas; variação genéticaThe isoenzymatic patterns of seven cultivars of elephant grass (Pennisetum purpureum) and their seven hybrids with Pearl millet (P. americanum), selected by Empresa Pernambucana de Pesquisa Agropecuária (IPA), were evaluated to identify the accesses. In polyacrylamide gels, the systems peroxidase (POX), esterase (EST), glutamate oxaloacetate transaminase (GOT), leucine amino peptidase (LAP), alcohol dehydrogenase (ADH), and acid phosphatase (ACP), were studied in young leaves at 28 days after uniformization cut. It wasn't observed isoenzymatic activity of ADH and the efficiency of the LAP system was considered low, which suggest that both can not be indicated for germoplasm characterization. The patterns ACP, GOT, POX and EST allowed the identification of the 14 accesses studied, which revealed 9, 3, 13 and 19 different electrophoretic patterns respectively. Using the esterase system alone, it is possible to identify the germoplasm bank of elephant grass quickly and accurately. Foram avaliadas isoenzimaticamente sete cultivares de capim-elefante (Pennisetum purpureum) e seus híbridos com milheto (P. americanum), selecionados pela Empresa Pernambucana de Pesquisa Agropecuária (IPA), visando à identificação de acessos. Foram estudados, em gel de poliacrilamida, os sistemas peroxidase (POX), esterase (EST), glutamato oxalacetato transaminase (GOT), leucina aminopeptidase (LAP), álcool-desidrogenase (ADH) e fosfatase ácida (ACP), em folhas jovens, aos 28 dias após o corte de uniformização. Não foi observada atividade isoenzimática da ADH e observou-se baixa resolução do sistema LAP, os quais não são indicados para caracterização dos germoplasmas. Os padrões de ACP, GOT, POX e EST permitiram conhecer os fenótipos dos 14 acessos estudados. Foram revelados 9, 3, 13 e 19 diferentes padrões de bandas, respectivamente, sendo possível a identificação da coleção de forma rápida e segura utilizando apenas os padrões de esterase.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraFreitas, Nara Suzi Aguiar deFalcão, Tânia Maria Muniz de ArrudaBurity, Hélio AlmeidaTabosa, José NildoSilva, Márcia Vânusa da2000-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/5901Pesquisa Agropecuaria Brasileira; v.35, n.6, jun. 2000; 1125-1133Pesquisa Agropecuária Brasileira; v.35, n.6, jun. 2000; 1125-11331678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/5901/3005info:eu-repo/semantics/openAccess2014-08-19T15:59:04Zoai:ojs.seer.sct.embrapa.br:article/5901Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-08-19T15:59:04Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Characterization and genetic diversity of elephant grass cultivars and their hybrids with millet through isoenzymatic patterns
Caracterização e diversidade genética do capim-elefante e seus híbridos com milheto mediante padrões isoenzimáticos
title Characterization and genetic diversity of elephant grass cultivars and their hybrids with millet through isoenzymatic patterns
spellingShingle Characterization and genetic diversity of elephant grass cultivars and their hybrids with millet through isoenzymatic patterns
Freitas, Nara Suzi Aguiar de
Pennisetum purpureum; Pennisetum americanum; gramineae; isoenzymes; genetic variation
Pennisetum purpureum; Pennisetum americanum; gramíneas; isoenzimas; variação genética
title_short Characterization and genetic diversity of elephant grass cultivars and their hybrids with millet through isoenzymatic patterns
title_full Characterization and genetic diversity of elephant grass cultivars and their hybrids with millet through isoenzymatic patterns
title_fullStr Characterization and genetic diversity of elephant grass cultivars and their hybrids with millet through isoenzymatic patterns
title_full_unstemmed Characterization and genetic diversity of elephant grass cultivars and their hybrids with millet through isoenzymatic patterns
title_sort Characterization and genetic diversity of elephant grass cultivars and their hybrids with millet through isoenzymatic patterns
author Freitas, Nara Suzi Aguiar de
author_facet Freitas, Nara Suzi Aguiar de
Falcão, Tânia Maria Muniz de Arruda
Burity, Hélio Almeida
Tabosa, José Nildo
Silva, Márcia Vânusa da
author_role author
author2 Falcão, Tânia Maria Muniz de Arruda
Burity, Hélio Almeida
Tabosa, José Nildo
Silva, Márcia Vânusa da
author2_role author
author
author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Freitas, Nara Suzi Aguiar de
Falcão, Tânia Maria Muniz de Arruda
Burity, Hélio Almeida
Tabosa, José Nildo
Silva, Márcia Vânusa da
dc.subject.por.fl_str_mv Pennisetum purpureum; Pennisetum americanum; gramineae; isoenzymes; genetic variation
Pennisetum purpureum; Pennisetum americanum; gramíneas; isoenzimas; variação genética
topic Pennisetum purpureum; Pennisetum americanum; gramineae; isoenzymes; genetic variation
Pennisetum purpureum; Pennisetum americanum; gramíneas; isoenzimas; variação genética
description The isoenzymatic patterns of seven cultivars of elephant grass (Pennisetum purpureum) and their seven hybrids with Pearl millet (P. americanum), selected by Empresa Pernambucana de Pesquisa Agropecuária (IPA), were evaluated to identify the accesses. In polyacrylamide gels, the systems peroxidase (POX), esterase (EST), glutamate oxaloacetate transaminase (GOT), leucine amino peptidase (LAP), alcohol dehydrogenase (ADH), and acid phosphatase (ACP), were studied in young leaves at 28 days after uniformization cut. It wasn't observed isoenzymatic activity of ADH and the efficiency of the LAP system was considered low, which suggest that both can not be indicated for germoplasm characterization. The patterns ACP, GOT, POX and EST allowed the identification of the 14 accesses studied, which revealed 9, 3, 13 and 19 different electrophoretic patterns respectively. Using the esterase system alone, it is possible to identify the germoplasm bank of elephant grass quickly and accurately.
publishDate 2000
dc.date.none.fl_str_mv 2000-06-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/5901
url https://seer.sct.embrapa.br/index.php/pab/article/view/5901
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/5901/3005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.35, n.6, jun. 2000; 1125-1133
Pesquisa Agropecuária Brasileira; v.35, n.6, jun. 2000; 1125-1133
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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