Endosperm genotyping as a strategy to differentiate the allele source in maize heterozygous progeny

Detalhes bibliográficos
Autor(a) principal: Martins, Francielle Alline
Data de Publicação: 2010
Outros Autores: Carneiro, Pedro Crescêncio Souza, Cruz, Cosme Damião, Carneiro, José Eustáquio de Souza, Guimarães, Claudia Teixeira
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/2200
Resumo: The objective of this work was to distinguish the parental source of alleles in heterozygous progeny using semiquantitative polymerase chain reaction (PCR) in maize endosperm. Endosperms derived from direct and reciprocal single-cross hybrids between maize inbred lines L3 and L1113-01 were genotyped by semiquantitative PCR methodology (SQ-PCR) using fluorescent microsatellite primers. The amplification products were evaluated by the ratios of fluorescence intensity (RFI), calculated between the peaks corresponding to the alleles derived from each parental line. Based on the statistically significant contrast between RFI mean values of direct and reciprocal single-cross hybrids, it was possible to distinguish the number of alleles received from each parental line and, ultimately, to determine the origin of the alleles of each cross. Thus, endosperm genotyping using SQ-PCR is a promising strategy to map QTL in maize outbred populations.
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spelling Endosperm genotyping as a strategy to differentiate the allele source in maize heterozygous progenyGenotipagem do endosperma como estratégia para diferenciar a origem de alelos em progênies heterozigóticas de milhoZea mays; allelic origin; heterozygote; natural populations; polygenes; semiquantitative PCRZea mays; origem alélica; heterozigoto; populações naturais; poligenes; PCR semiquantitativaThe objective of this work was to distinguish the parental source of alleles in heterozygous progeny using semiquantitative polymerase chain reaction (PCR) in maize endosperm. Endosperms derived from direct and reciprocal single-cross hybrids between maize inbred lines L3 and L1113-01 were genotyped by semiquantitative PCR methodology (SQ-PCR) using fluorescent microsatellite primers. The amplification products were evaluated by the ratios of fluorescence intensity (RFI), calculated between the peaks corresponding to the alleles derived from each parental line. Based on the statistically significant contrast between RFI mean values of direct and reciprocal single-cross hybrids, it was possible to distinguish the number of alleles received from each parental line and, ultimately, to determine the origin of the alleles of each cross. Thus, endosperm genotyping using SQ-PCR is a promising strategy to map QTL in maize outbred populations.O objetivo deste trabalho foi distinguir a origem de alelos em progênies heterozigóticas usando reação em cadeia da polimerase (PCR) semiquantitativa em endosperma de milho. Endospermas derivados de híbridos simples diretos e recíprocos entre as linhagens de milho L3 e L1113-01 foram genotipados pela metodologia de PCR semiquantitativa (PCR-SQ) com uso de primers microssatélites fluorescentes. Os produtos de amplificação foram avaliados por meio da razão de intensidade de fluorescência (RIF), calculada entre os valores de intensidade dos picos correspondentes aos alelos derivados de cada genitor. Com base no contraste estatisticamente significativo dos valores médios das RIF entre os híbridos simples direto e recíproco, foi possível distinguir o número de alelos recebidos de cada genitor e, finalmente, determinar a origem dos alelos de cada híbrido. Assim, a genotipagem de endosperma utilizando PCR-SQ é uma estratégia promissora no mapeamento de QTLs em populações exogâmicas de milho.    Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraFAPEMIG E CNPqMartins, Francielle AllineCarneiro, Pedro Crescêncio SouzaCruz, Cosme DamiãoCarneiro, José Eustáquio de SouzaGuimarães, Claudia Teixeira2010-12-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/2200Pesquisa Agropecuaria Brasileira; v.44, n.10, out. 2009; 1291-1296Pesquisa Agropecuária Brasileira; v.44, n.10, out. 2009; 1291-12961678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/2200/5842https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/2200/1654info:eu-repo/semantics/openAccess2012-06-17T11:53:41Zoai:ojs.seer.sct.embrapa.br:article/2200Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2012-06-17T11:53:41Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Endosperm genotyping as a strategy to differentiate the allele source in maize heterozygous progeny
Genotipagem do endosperma como estratégia para diferenciar a origem de alelos em progênies heterozigóticas de milho
title Endosperm genotyping as a strategy to differentiate the allele source in maize heterozygous progeny
spellingShingle Endosperm genotyping as a strategy to differentiate the allele source in maize heterozygous progeny
Martins, Francielle Alline
Zea mays; allelic origin; heterozygote; natural populations; polygenes; semiquantitative PCR
Zea mays; origem alélica; heterozigoto; populações naturais; poligenes; PCR semiquantitativa
title_short Endosperm genotyping as a strategy to differentiate the allele source in maize heterozygous progeny
title_full Endosperm genotyping as a strategy to differentiate the allele source in maize heterozygous progeny
title_fullStr Endosperm genotyping as a strategy to differentiate the allele source in maize heterozygous progeny
title_full_unstemmed Endosperm genotyping as a strategy to differentiate the allele source in maize heterozygous progeny
title_sort Endosperm genotyping as a strategy to differentiate the allele source in maize heterozygous progeny
author Martins, Francielle Alline
author_facet Martins, Francielle Alline
Carneiro, Pedro Crescêncio Souza
Cruz, Cosme Damião
Carneiro, José Eustáquio de Souza
Guimarães, Claudia Teixeira
author_role author
author2 Carneiro, Pedro Crescêncio Souza
Cruz, Cosme Damião
Carneiro, José Eustáquio de Souza
Guimarães, Claudia Teixeira
author2_role author
author
author
author
dc.contributor.none.fl_str_mv
FAPEMIG E CNPq
dc.contributor.author.fl_str_mv Martins, Francielle Alline
Carneiro, Pedro Crescêncio Souza
Cruz, Cosme Damião
Carneiro, José Eustáquio de Souza
Guimarães, Claudia Teixeira
dc.subject.por.fl_str_mv Zea mays; allelic origin; heterozygote; natural populations; polygenes; semiquantitative PCR
Zea mays; origem alélica; heterozigoto; populações naturais; poligenes; PCR semiquantitativa
topic Zea mays; allelic origin; heterozygote; natural populations; polygenes; semiquantitative PCR
Zea mays; origem alélica; heterozigoto; populações naturais; poligenes; PCR semiquantitativa
description The objective of this work was to distinguish the parental source of alleles in heterozygous progeny using semiquantitative polymerase chain reaction (PCR) in maize endosperm. Endosperms derived from direct and reciprocal single-cross hybrids between maize inbred lines L3 and L1113-01 were genotyped by semiquantitative PCR methodology (SQ-PCR) using fluorescent microsatellite primers. The amplification products were evaluated by the ratios of fluorescence intensity (RFI), calculated between the peaks corresponding to the alleles derived from each parental line. Based on the statistically significant contrast between RFI mean values of direct and reciprocal single-cross hybrids, it was possible to distinguish the number of alleles received from each parental line and, ultimately, to determine the origin of the alleles of each cross. Thus, endosperm genotyping using SQ-PCR is a promising strategy to map QTL in maize outbred populations.
publishDate 2010
dc.date.none.fl_str_mv 2010-12-03
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/2200
url https://seer.sct.embrapa.br/index.php/pab/article/view/2200
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/2200/5842
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/2200/1654
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.44, n.10, out. 2009; 1291-1296
Pesquisa Agropecuária Brasileira; v.44, n.10, out. 2009; 1291-1296
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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