Dicyma pulvinata isolates colonizing Microcyclus ulei stromata in rubber
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Pesquisa Agropecuária Brasileira (Online) |
Texto Completo: | https://seer.sct.embrapa.br/index.php/pab/article/view/7149 |
Resumo: | Dicyma pulvinata is an efficient biocontrol agent of Microcyclus ulei, causal agent of South American leaf blight. The objective of this work was to obtain strongly antagonistic isolates. A survey was carried out in rubber plantations located in 14 municipalities in the Sates of Acre, Amazonas, Bahia, Mato Grosso, Pará and Roraima, Brazil, obtaining 52 isolates of D. pulvinata. The antagonistic fungus was isolated on potato-dextrose-agar (PDA) plates and identified based on morphological characteristics of conidiophores and conidia, and colony appearance. The antagonic effect was tested by inoculating D. pulvinata on Fusicladium macrosporum lesions induced on rubber plants, previously. In order to maintain the morphological and pathogenic traits, fungus isolates were preserved by lyophilization, deep freeze (-80oC) and cryogeny. All isolates were introduced into Embrapa Recursos Genéticos e Biotecnologia fungus collection. |
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Dicyma pulvinata isolates colonizing Microcyclus ulei stromata in rubberIsolados de Dicyma pulvinata em estromas de Microcyclus ulei em seringueiraHevea; biological control; antagonist fungiHevea; controle biológico; fungo antagonistaDicyma pulvinata is an efficient biocontrol agent of Microcyclus ulei, causal agent of South American leaf blight. The objective of this work was to obtain strongly antagonistic isolates. A survey was carried out in rubber plantations located in 14 municipalities in the Sates of Acre, Amazonas, Bahia, Mato Grosso, Pará and Roraima, Brazil, obtaining 52 isolates of D. pulvinata. The antagonistic fungus was isolated on potato-dextrose-agar (PDA) plates and identified based on morphological characteristics of conidiophores and conidia, and colony appearance. The antagonic effect was tested by inoculating D. pulvinata on Fusicladium macrosporum lesions induced on rubber plants, previously. In order to maintain the morphological and pathogenic traits, fungus isolates were preserved by lyophilization, deep freeze (-80oC) and cryogeny. All isolates were introduced into Embrapa Recursos Genéticos e Biotecnologia fungus collection.Dicyma pulvinata é um eficiente agente de biocontrole de Microcyclus ulei, causador do mal-das-folhas da seringueira. O objetivo deste trabalho foi obter isolados com grande potencial antagônico. Em levantamentos realizados em 14 municípios produtores de borracha, localizados nos Estados do Acre, Amazonas, Bahia, Mato Grosso, Pará e Rondônia, obtiveram-se 52 isolados de D. pulvinata. O fungo antagônico, isolado diretamente em meio de cultura de batata-dextrose-ágar (BDA), foi identificado com base na morfologia dos conídios e conidióforos e preservado pelos métodos da liofilização, congelamento à temperatura de -80oC e criopreservação em nitrogênio líquido, a fim de manter as características morfológicas e patogênicas dos isolados. O efeito antagônico foi testado por meio de inoculações de D. pulvinata em lesões de Fusicladium macrosporum induzidas em plantas de seringueira previamente infectadas. Todos os isolados foram incorporados à coleção de fungos da Embrapa Recursos Genéticos e Biotecnologia.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraMello, Sueli Corrêa Marques deSantos, Maria de FátimaSilva, João Batista Tavares da2006-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/7149Pesquisa Agropecuaria Brasileira; v.41, n.2, fev. 2006; 359-363Pesquisa Agropecuária Brasileira; v.41, n.2, fev. 2006; 359-3631678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/7149/4194info:eu-repo/semantics/openAccess2014-09-26T17:38:31Zoai:ojs.seer.sct.embrapa.br:article/7149Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-09-26T17:38:31Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Dicyma pulvinata isolates colonizing Microcyclus ulei stromata in rubber Isolados de Dicyma pulvinata em estromas de Microcyclus ulei em seringueira |
title |
Dicyma pulvinata isolates colonizing Microcyclus ulei stromata in rubber |
spellingShingle |
Dicyma pulvinata isolates colonizing Microcyclus ulei stromata in rubber Mello, Sueli Corrêa Marques de Hevea; biological control; antagonist fungi Hevea; controle biológico; fungo antagonista |
title_short |
Dicyma pulvinata isolates colonizing Microcyclus ulei stromata in rubber |
title_full |
Dicyma pulvinata isolates colonizing Microcyclus ulei stromata in rubber |
title_fullStr |
Dicyma pulvinata isolates colonizing Microcyclus ulei stromata in rubber |
title_full_unstemmed |
Dicyma pulvinata isolates colonizing Microcyclus ulei stromata in rubber |
title_sort |
Dicyma pulvinata isolates colonizing Microcyclus ulei stromata in rubber |
author |
Mello, Sueli Corrêa Marques de |
author_facet |
Mello, Sueli Corrêa Marques de Santos, Maria de Fátima Silva, João Batista Tavares da |
author_role |
author |
author2 |
Santos, Maria de Fátima Silva, João Batista Tavares da |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
|
dc.contributor.author.fl_str_mv |
Mello, Sueli Corrêa Marques de Santos, Maria de Fátima Silva, João Batista Tavares da |
dc.subject.por.fl_str_mv |
Hevea; biological control; antagonist fungi Hevea; controle biológico; fungo antagonista |
topic |
Hevea; biological control; antagonist fungi Hevea; controle biológico; fungo antagonista |
description |
Dicyma pulvinata is an efficient biocontrol agent of Microcyclus ulei, causal agent of South American leaf blight. The objective of this work was to obtain strongly antagonistic isolates. A survey was carried out in rubber plantations located in 14 municipalities in the Sates of Acre, Amazonas, Bahia, Mato Grosso, Pará and Roraima, Brazil, obtaining 52 isolates of D. pulvinata. The antagonistic fungus was isolated on potato-dextrose-agar (PDA) plates and identified based on morphological characteristics of conidiophores and conidia, and colony appearance. The antagonic effect was tested by inoculating D. pulvinata on Fusicladium macrosporum lesions induced on rubber plants, previously. In order to maintain the morphological and pathogenic traits, fungus isolates were preserved by lyophilization, deep freeze (-80oC) and cryogeny. All isolates were introduced into Embrapa Recursos Genéticos e Biotecnologia fungus collection. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-02-01 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/7149 |
url |
https://seer.sct.embrapa.br/index.php/pab/article/view/7149 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/7149/4194 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
dc.source.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira; v.41, n.2, fev. 2006; 359-363 Pesquisa Agropecuária Brasileira; v.41, n.2, fev. 2006; 359-363 1678-3921 0100-104x reponame:Pesquisa Agropecuária Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Agropecuária Brasileira (Online) |
collection |
Pesquisa Agropecuária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
pab@sct.embrapa.br || sct.pab@embrapa.br |
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1793416656040493056 |