Dicyma pulvinata isolates colonizing Microcyclus ulei stromata in rubber

Detalhes bibliográficos
Autor(a) principal: Mello, Sueli Corrêa Marques de
Data de Publicação: 2006
Outros Autores: Santos, Maria de Fátima, Silva, João Batista Tavares da
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/7149
Resumo: Dicyma pulvinata is an efficient biocontrol agent of Microcyclus ulei, causal agent of South American leaf blight. The objective of this work was to obtain strongly antagonistic isolates. A survey was carried out in rubber plantations located in 14 municipalities in the Sates of Acre, Amazonas, Bahia, Mato Grosso, Pará and Roraima, Brazil, obtaining 52 isolates of D. pulvinata. The antagonistic fungus was isolated on potato-dextrose-agar (PDA) plates and identified based on morphological characteristics of conidiophores and conidia, and colony appearance. The antagonic effect was tested by inoculating D. pulvinata on Fusicladium macrosporum lesions induced on rubber plants, previously. In order to maintain the morphological and pathogenic traits, fungus isolates were preserved by lyophilization, deep freeze (-80oC) and cryogeny. All isolates were introduced into Embrapa Recursos Genéticos e Biotecnologia fungus collection.
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spelling Dicyma pulvinata isolates colonizing Microcyclus ulei stromata in rubberIsolados de Dicyma pulvinata em estromas de Microcyclus ulei em seringueiraHevea; biological control; antagonist fungiHevea; controle biológico; fungo antagonistaDicyma pulvinata is an efficient biocontrol agent of Microcyclus ulei, causal agent of South American leaf blight. The objective of this work was to obtain strongly antagonistic isolates. A survey was carried out in rubber plantations located in 14 municipalities in the Sates of Acre, Amazonas, Bahia, Mato Grosso, Pará and Roraima, Brazil, obtaining 52 isolates of D. pulvinata. The antagonistic fungus was isolated on potato-dextrose-agar (PDA) plates and identified based on morphological characteristics of conidiophores and conidia, and colony appearance. The antagonic effect was tested by inoculating D. pulvinata on Fusicladium macrosporum lesions induced on rubber plants, previously. In order to maintain the morphological and pathogenic traits, fungus isolates were preserved by lyophilization, deep freeze (-80oC) and cryogeny. All isolates were introduced into Embrapa Recursos Genéticos e Biotecnologia fungus collection.Dicyma pulvinata é um eficiente agente de biocontrole de Microcyclus ulei, causador do mal-das-folhas da seringueira. O objetivo deste trabalho foi obter isolados com grande potencial antagônico. Em levantamentos realizados em 14 municípios produtores de borracha, localizados nos Estados do Acre, Amazonas, Bahia, Mato Grosso, Pará e Rondônia, obtiveram-se 52 isolados de D. pulvinata. O fungo antagônico, isolado diretamente em meio de cultura de batata-dextrose-ágar (BDA), foi identificado com base na morfologia dos conídios e conidióforos e preservado pelos métodos da liofilização, congelamento à temperatura de -80oC e criopreservação em nitrogênio líquido, a fim de manter as características morfológicas e patogênicas dos isolados. O efeito antagônico foi testado por meio de inoculações de D. pulvinata em lesões de Fusicladium macrosporum induzidas em plantas de seringueira previamente infectadas. Todos os isolados foram incorporados à coleção de fungos da Embrapa Recursos Genéticos e Biotecnologia.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraMello, Sueli Corrêa Marques deSantos, Maria de FátimaSilva, João Batista Tavares da2006-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/7149Pesquisa Agropecuaria Brasileira; v.41, n.2, fev. 2006; 359-363Pesquisa Agropecuária Brasileira; v.41, n.2, fev. 2006; 359-3631678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/7149/4194info:eu-repo/semantics/openAccess2014-09-26T17:38:31Zoai:ojs.seer.sct.embrapa.br:article/7149Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-09-26T17:38:31Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Dicyma pulvinata isolates colonizing Microcyclus ulei stromata in rubber
Isolados de Dicyma pulvinata em estromas de Microcyclus ulei em seringueira
title Dicyma pulvinata isolates colonizing Microcyclus ulei stromata in rubber
spellingShingle Dicyma pulvinata isolates colonizing Microcyclus ulei stromata in rubber
Mello, Sueli Corrêa Marques de
Hevea; biological control; antagonist fungi
Hevea; controle biológico; fungo antagonista
title_short Dicyma pulvinata isolates colonizing Microcyclus ulei stromata in rubber
title_full Dicyma pulvinata isolates colonizing Microcyclus ulei stromata in rubber
title_fullStr Dicyma pulvinata isolates colonizing Microcyclus ulei stromata in rubber
title_full_unstemmed Dicyma pulvinata isolates colonizing Microcyclus ulei stromata in rubber
title_sort Dicyma pulvinata isolates colonizing Microcyclus ulei stromata in rubber
author Mello, Sueli Corrêa Marques de
author_facet Mello, Sueli Corrêa Marques de
Santos, Maria de Fátima
Silva, João Batista Tavares da
author_role author
author2 Santos, Maria de Fátima
Silva, João Batista Tavares da
author2_role author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Mello, Sueli Corrêa Marques de
Santos, Maria de Fátima
Silva, João Batista Tavares da
dc.subject.por.fl_str_mv Hevea; biological control; antagonist fungi
Hevea; controle biológico; fungo antagonista
topic Hevea; biological control; antagonist fungi
Hevea; controle biológico; fungo antagonista
description Dicyma pulvinata is an efficient biocontrol agent of Microcyclus ulei, causal agent of South American leaf blight. The objective of this work was to obtain strongly antagonistic isolates. A survey was carried out in rubber plantations located in 14 municipalities in the Sates of Acre, Amazonas, Bahia, Mato Grosso, Pará and Roraima, Brazil, obtaining 52 isolates of D. pulvinata. The antagonistic fungus was isolated on potato-dextrose-agar (PDA) plates and identified based on morphological characteristics of conidiophores and conidia, and colony appearance. The antagonic effect was tested by inoculating D. pulvinata on Fusicladium macrosporum lesions induced on rubber plants, previously. In order to maintain the morphological and pathogenic traits, fungus isolates were preserved by lyophilization, deep freeze (-80oC) and cryogeny. All isolates were introduced into Embrapa Recursos Genéticos e Biotecnologia fungus collection.
publishDate 2006
dc.date.none.fl_str_mv 2006-02-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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format article
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dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/7149
url https://seer.sct.embrapa.br/index.php/pab/article/view/7149
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/7149/4194
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.41, n.2, fev. 2006; 359-363
Pesquisa Agropecuária Brasileira; v.41, n.2, fev. 2006; 359-363
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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