Genetic variability estimated in banana diploids through microsatellite markers

Detalhes bibliográficos
Autor(a) principal: Amorim, Edson Perito
Data de Publicação: 2008
Outros Autores: Reis, Ronaldo Viana dos, Santos-Serejo, Janay Almeida dos, Amorim, Vanusia Batista de Oliveira, Silva, Sebastião de Oliveira e
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/490
Resumo: The objective of this work was to estimate the genetic diversity between 38 banana diploids from the banana breeding program of Embrapa Mandioca e Fruticultura Tropical (Embrapa Cassava and Tropical Fruits), including improved hybrids, cultivated and wild species, using fi fteen SSR markers. Genetic similarities were utilized to cluster genotypes through UPGMA, based on Jaccard coeffi cient. The average number of alleles per primer was 7.53, with a total of 113 alleles identifi ed. The average similarity was 0.22, and ranged from 0.028 to 0.48, indicating the existence of genetic variability between genotypes. The clustering analysis based on microsatellite polymorphism could not completely separate improved hybrids, cultivated and wild species.Some diploids grouped according to their geographic origins, among which Musa ornata and IAC-1, and Tjau Lagada and Lidi, while in others no relation was established. There was a trend of clustering among related diploids as: SH3263 and 8694-20; 4279-01 and 9179-03; 1304-06 and 5854-03; 86B79-10 and 7341-03; 86B79-12 and 0337-02; and 9194-04 and 4154-08.
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spelling Genetic variability estimated in banana diploids through microsatellite markersVariabilidade genética estimada entre diplóides de banana por meio de marcadores microssatélitesMusa; diploids; genetic diversity; hybrids; molecular markersMusa; diplóides; divergência genética; híbridos; marcadores molecularesThe objective of this work was to estimate the genetic diversity between 38 banana diploids from the banana breeding program of Embrapa Mandioca e Fruticultura Tropical (Embrapa Cassava and Tropical Fruits), including improved hybrids, cultivated and wild species, using fi fteen SSR markers. Genetic similarities were utilized to cluster genotypes through UPGMA, based on Jaccard coeffi cient. The average number of alleles per primer was 7.53, with a total of 113 alleles identifi ed. The average similarity was 0.22, and ranged from 0.028 to 0.48, indicating the existence of genetic variability between genotypes. The clustering analysis based on microsatellite polymorphism could not completely separate improved hybrids, cultivated and wild species.Some diploids grouped according to their geographic origins, among which Musa ornata and IAC-1, and Tjau Lagada and Lidi, while in others no relation was established. There was a trend of clustering among related diploids as: SH3263 and 8694-20; 4279-01 and 9179-03; 1304-06 and 5854-03; 86B79-10 and 7341-03; 86B79-12 and 0337-02; and 9194-04 and 4154-08.O objetivo deste trabalho foi estimar a divergência genética entre 38 diplóides de banana do programa de melhoramento da Embrapa Mandioca e Fruticultura Tropical, incluindo genótipos melhorados, cultivadose selvagens, por meio de 15 marcadores microssatélites ou SSR. As similaridades genéticas, com base no coefi ciente de Jaccard, foram utilizadas para fazer o agrupamento dos genótipos pelo método UPGMA.O número de alelos obtidos foi 113, com média de 7,53 alelos por iniciador. A similaridade genética média foi de 0,22, e variou de 0,028 a 0,48, o que indica existência de variabilidade genética entre os genótipos. A análise de grupos, com base no polimorfi smo de microssatélites, não pôde separar completamente os híbridosmelhorados, cultivados e selvagens. Alguns diplóides agruparam-se com base em sua origem geográfi ca, entre eles Musa ornata e IAC-1, e Tjau Lagada e Lidi, enquanto que, em outros, nenhuma relação foi estabelecida.Houve tendência de agrupamento entre os diplóides aparentados, como: SH3263 e 8694-20, 4279-01 e 9179-03,1304-06 e 5854-03, 86B79-10 e 7341-03, 86B79-12 e 0337-02, e 9194-04 e 4154-08.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraCNPqAmorim, Edson PeritoReis, Ronaldo Viana dosSantos-Serejo, Janay Almeida dosAmorim, Vanusia Batista de OliveiraSilva, Sebastião de Oliveira e2008-10-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/490Pesquisa Agropecuaria Brasileira; v.43, n.8, ago. 2008; 1045-1052Pesquisa Agropecuária Brasileira; v.43, n.8, ago. 2008; 1045-10521678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/490/5614info:eu-repo/semantics/openAccess2014-07-16T18:56:52Zoai:ojs.seer.sct.embrapa.br:article/490Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-07-16T18:56:52Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Genetic variability estimated in banana diploids through microsatellite markers
Variabilidade genética estimada entre diplóides de banana por meio de marcadores microssatélites
title Genetic variability estimated in banana diploids through microsatellite markers
spellingShingle Genetic variability estimated in banana diploids through microsatellite markers
Amorim, Edson Perito
Musa; diploids; genetic diversity; hybrids; molecular markers
Musa; diplóides; divergência genética; híbridos; marcadores moleculares
title_short Genetic variability estimated in banana diploids through microsatellite markers
title_full Genetic variability estimated in banana diploids through microsatellite markers
title_fullStr Genetic variability estimated in banana diploids through microsatellite markers
title_full_unstemmed Genetic variability estimated in banana diploids through microsatellite markers
title_sort Genetic variability estimated in banana diploids through microsatellite markers
author Amorim, Edson Perito
author_facet Amorim, Edson Perito
Reis, Ronaldo Viana dos
Santos-Serejo, Janay Almeida dos
Amorim, Vanusia Batista de Oliveira
Silva, Sebastião de Oliveira e
author_role author
author2 Reis, Ronaldo Viana dos
Santos-Serejo, Janay Almeida dos
Amorim, Vanusia Batista de Oliveira
Silva, Sebastião de Oliveira e
author2_role author
author
author
author
dc.contributor.none.fl_str_mv
CNPq
dc.contributor.author.fl_str_mv Amorim, Edson Perito
Reis, Ronaldo Viana dos
Santos-Serejo, Janay Almeida dos
Amorim, Vanusia Batista de Oliveira
Silva, Sebastião de Oliveira e
dc.subject.por.fl_str_mv Musa; diploids; genetic diversity; hybrids; molecular markers
Musa; diplóides; divergência genética; híbridos; marcadores moleculares
topic Musa; diploids; genetic diversity; hybrids; molecular markers
Musa; diplóides; divergência genética; híbridos; marcadores moleculares
description The objective of this work was to estimate the genetic diversity between 38 banana diploids from the banana breeding program of Embrapa Mandioca e Fruticultura Tropical (Embrapa Cassava and Tropical Fruits), including improved hybrids, cultivated and wild species, using fi fteen SSR markers. Genetic similarities were utilized to cluster genotypes through UPGMA, based on Jaccard coeffi cient. The average number of alleles per primer was 7.53, with a total of 113 alleles identifi ed. The average similarity was 0.22, and ranged from 0.028 to 0.48, indicating the existence of genetic variability between genotypes. The clustering analysis based on microsatellite polymorphism could not completely separate improved hybrids, cultivated and wild species.Some diploids grouped according to their geographic origins, among which Musa ornata and IAC-1, and Tjau Lagada and Lidi, while in others no relation was established. There was a trend of clustering among related diploids as: SH3263 and 8694-20; 4279-01 and 9179-03; 1304-06 and 5854-03; 86B79-10 and 7341-03; 86B79-12 and 0337-02; and 9194-04 and 4154-08.
publishDate 2008
dc.date.none.fl_str_mv 2008-10-28
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/490
url https://seer.sct.embrapa.br/index.php/pab/article/view/490
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/490/5614
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.43, n.8, ago. 2008; 1045-1052
Pesquisa Agropecuária Brasileira; v.43, n.8, ago. 2008; 1045-1052
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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