Organogenesis of the leaf explant of Eucalyptus grandis x E. urophylla clones

Detalhes bibliográficos
Autor(a) principal: Alves, Elisa Cristina Soares de Carvalho
Data de Publicação: 2010
Outros Autores: Xavier, Aloísio, Otoni, Wagner Campos
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/9549
Resumo: The aim of this work was to evaluate the effects of growth regulators TDZ [1-phenil-3-(1,2,3-thiadiazol-5-yl)urea], BAP (6-benzilaminopurine) e NAA (Naphthalene acetic acid) on the in vitro propagation by organogenesis from foliar explants of Eucalyptus grandis x E. urophylla. Depending on the clone used, there were singular responses to growth regulators treatment regarding callusing intensity, texture and color. The best results of the three genotypes used were observed with the TDZ (0.5 mg L-1) and NAA (0.1 mg L-1) treatment, where 100% of the foliar explants presented callus. The worst results were observed with the BAP (0.1 mg L-1) and NAA (0.1 mg L-1) treatment. Subsequently, considering the regeneration process, the best response was achieved with 1.0 mg L-1 BAP, in which 8% of the calli regenerated buds, with an average of 4.2 buds per explant.
id EMBRAPA-4_45b52fef2326bbb7ec4a8e8412b2d7b5
oai_identifier_str oai:ojs.seer.sct.embrapa.br:article/9549
network_acronym_str EMBRAPA-4
network_name_str Pesquisa Agropecuária Brasileira (Online)
repository_id_str
spelling Organogenesis of the leaf explant of Eucalyptus grandis x E. urophylla clonesOrganogênese de explante foliar de clones de Eucalyptus grandis x E. urophyllamicropropagation; growth regulators; in vitro propagationmicropropagação; reguladores de crescimento; propagação in vitroThe aim of this work was to evaluate the effects of growth regulators TDZ [1-phenil-3-(1,2,3-thiadiazol-5-yl)urea], BAP (6-benzilaminopurine) e NAA (Naphthalene acetic acid) on the in vitro propagation by organogenesis from foliar explants of Eucalyptus grandis x E. urophylla. Depending on the clone used, there were singular responses to growth regulators treatment regarding callusing intensity, texture and color. The best results of the three genotypes used were observed with the TDZ (0.5 mg L-1) and NAA (0.1 mg L-1) treatment, where 100% of the foliar explants presented callus. The worst results were observed with the BAP (0.1 mg L-1) and NAA (0.1 mg L-1) treatment. Subsequently, considering the regeneration process, the best response was achieved with 1.0 mg L-1 BAP, in which 8% of the calli regenerated buds, with an average of 4.2 buds per explant.O objetivo deste trabalho foi avaliar os efeitos dos reguladores de crescimento TDZ [1-fenil-3-(1,2,3-tiadiazol-5-il)uréia], BAP (6-benzilaminopurina) e ANA (ácido naftalenoacético) no desempenho da propagação in vitro por organogênese de explante foliar de três clones híbridos de Eucalyptus grandis x Eucalyptus urophylla. Houve resposta diferenciada dos clones quanto a intensidade, textura e coloração dos calos, em razão dos tratamentos com os reguladores de crescimento. Os melhores resultados de calejamento dos três genótipos foram observados nos tratamentos com a combinação dos reguladores de crescimento TDZ (0,5 mg L-1) e ANA (0,1 mg L-1), obtendo-se 100% de calejamento no explante foliar. Os piores resultados de calejamento foram observados nos tratamentos com a combinação dos reguladores de crescimento BAP (0,1 mg L-1) e ANA (0,1 mg L-1). Em relação à regeneração, a melhor resposta foi obtida com 1,0 mg L-1 BAP em que 8% dos calos formados a partir de explantes foliares regeneraram gemas, com número médio destas formadas por calo igual a 4,2.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraAlves, Elisa Cristina Soares de CarvalhoXavier, AloísioOtoni, Wagner Campos2010-10-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/9549Pesquisa Agropecuaria Brasileira; v.39, n.5, maio 2004; 421-430Pesquisa Agropecuária Brasileira; v.39, n.5, maio 2004; 421-4301678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/9549/5380info:eu-repo/semantics/openAccess2014-07-17T19:23:44Zoai:ojs.seer.sct.embrapa.br:article/9549Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-07-17T19:23:44Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Organogenesis of the leaf explant of Eucalyptus grandis x E. urophylla clones
Organogênese de explante foliar de clones de Eucalyptus grandis x E. urophylla
title Organogenesis of the leaf explant of Eucalyptus grandis x E. urophylla clones
spellingShingle Organogenesis of the leaf explant of Eucalyptus grandis x E. urophylla clones
Alves, Elisa Cristina Soares de Carvalho
micropropagation; growth regulators; in vitro propagation
micropropagação; reguladores de crescimento; propagação in vitro
title_short Organogenesis of the leaf explant of Eucalyptus grandis x E. urophylla clones
title_full Organogenesis of the leaf explant of Eucalyptus grandis x E. urophylla clones
title_fullStr Organogenesis of the leaf explant of Eucalyptus grandis x E. urophylla clones
title_full_unstemmed Organogenesis of the leaf explant of Eucalyptus grandis x E. urophylla clones
title_sort Organogenesis of the leaf explant of Eucalyptus grandis x E. urophylla clones
author Alves, Elisa Cristina Soares de Carvalho
author_facet Alves, Elisa Cristina Soares de Carvalho
Xavier, Aloísio
Otoni, Wagner Campos
author_role author
author2 Xavier, Aloísio
Otoni, Wagner Campos
author2_role author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Alves, Elisa Cristina Soares de Carvalho
Xavier, Aloísio
Otoni, Wagner Campos
dc.subject.por.fl_str_mv micropropagation; growth regulators; in vitro propagation
micropropagação; reguladores de crescimento; propagação in vitro
topic micropropagation; growth regulators; in vitro propagation
micropropagação; reguladores de crescimento; propagação in vitro
description The aim of this work was to evaluate the effects of growth regulators TDZ [1-phenil-3-(1,2,3-thiadiazol-5-yl)urea], BAP (6-benzilaminopurine) e NAA (Naphthalene acetic acid) on the in vitro propagation by organogenesis from foliar explants of Eucalyptus grandis x E. urophylla. Depending on the clone used, there were singular responses to growth regulators treatment regarding callusing intensity, texture and color. The best results of the three genotypes used were observed with the TDZ (0.5 mg L-1) and NAA (0.1 mg L-1) treatment, where 100% of the foliar explants presented callus. The worst results were observed with the BAP (0.1 mg L-1) and NAA (0.1 mg L-1) treatment. Subsequently, considering the regeneration process, the best response was achieved with 1.0 mg L-1 BAP, in which 8% of the calli regenerated buds, with an average of 4.2 buds per explant.
publishDate 2010
dc.date.none.fl_str_mv 2010-10-06
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/9549
url https://seer.sct.embrapa.br/index.php/pab/article/view/9549
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/9549/5380
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.39, n.5, maio 2004; 421-430
Pesquisa Agropecuária Brasileira; v.39, n.5, maio 2004; 421-430
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
_version_ 1793416685455147008

Registros relacionados