Production technology for entomopathogenic fungus using a biphasic culture system

Detalhes bibliográficos
Autor(a) principal: Machado, Ana Carolina Ribeiro
Data de Publicação: 2011
Outros Autores: Monteiro, Antonio Carlos, de Almeida, Aline Maria Belasco, Geraldo Martins, Maria Inez Espagnoli
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/8575
Resumo: The objective of this research was to evaluate combinations of liquid media obtained from agro-industrial residues and by-products, with solid media prepared with mixtures of grains and their derivatives, aiming to increase the production of JAB 02 and JAB 45 isolates of Lecanicillium lecanii. Sporulation, conidial viability and process yield were evaluated as well as the production costs using the JAB 45 isolate as a model system were analyzed. The production of JAB 02 was not increased using the biphasic culture. For JAB 45, some combinations provided an increase in yield, especially cheese whey with wheat bran and wheat grain, with lower production costs. Viability was not influenced by the production method, and the combinations showed no differences in the process yield. The biphasic method is suitable for the production of L. lecanii, and proves to be an appropriate technology to use in mass production by biofactories.
id EMBRAPA-4_4fc57744b4a413f94b50bd8111308c94
oai_identifier_str oai:ojs.seer.sct.embrapa.br:article/8575
network_acronym_str EMBRAPA-4
network_name_str Pesquisa Agropecuária Brasileira (Online)
repository_id_str
spelling Production technology for entomopathogenic fungus using a biphasic culture systemTecnologia de produção de fungo entomopatogênico pelo sistema bifásico de cultivoLecanicillium lecanii; conidial viability; liquid media; mass production; solid mediaLecanicillium lecanii; viabilidade de conídios; meios líquidos; produção massal; meios sólidosThe objective of this research was to evaluate combinations of liquid media obtained from agro-industrial residues and by-products, with solid media prepared with mixtures of grains and their derivatives, aiming to increase the production of JAB 02 and JAB 45 isolates of Lecanicillium lecanii. Sporulation, conidial viability and process yield were evaluated as well as the production costs using the JAB 45 isolate as a model system were analyzed. The production of JAB 02 was not increased using the biphasic culture. For JAB 45, some combinations provided an increase in yield, especially cheese whey with wheat bran and wheat grain, with lower production costs. Viability was not influenced by the production method, and the combinations showed no differences in the process yield. The biphasic method is suitable for the production of L. lecanii, and proves to be an appropriate technology to use in mass production by biofactories.O objetivo deste trabalho foi avaliar combinações de meios líquidos, obtidos de resíduos e subprodutos agroindustriais, e meios sólidos, preparados com misturas de grãos e seus derivados, visando o aumento da produção dos isolados JAB 02 e JAB 45 de Lecanicillium lecanii. Avaliaram-se a esporulação, a viabilidade de conídios e o rendimento do processo, bem como o custo de produção usando como modelo o isolado JAB 45 foi analisado. A produção de JAB 02 não foi incrementada pelo cultivo bifásico. Para JAB 45, algumas combinações promoveram aumento na produção, especialmente a de soro de queijo com farelo de trigo e trigo em grão, que apresentou o menor custo. A viabilidade não foi influenciada pelo método de produção e as combinações não mostraram diferenças quanto ao rendimento do processo. O método bifásico é adequado para a produção de L. lecanii, sendo uma tecnologia apropriada para uso com o objetivo de produção massal por biofábricas.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraCAPESMachado, Ana Carolina RibeiroMonteiro, Antonio Carlosde Almeida, Aline Maria BelascoGeraldo Martins, Maria Inez Espagnoli2011-01-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/8575Pesquisa Agropecuaria Brasileira; v.45, n.10, out. 2010; 1157-1163Pesquisa Agropecuária Brasileira; v.45, n.10, out. 2010; 1157-11631678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/8575/6126https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/8575/4297info:eu-repo/semantics/openAccess2014-11-12T17:21:58Zoai:ojs.seer.sct.embrapa.br:article/8575Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-11-12T17:21:58Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Production technology for entomopathogenic fungus using a biphasic culture system
Tecnologia de produção de fungo entomopatogênico pelo sistema bifásico de cultivo
title Production technology for entomopathogenic fungus using a biphasic culture system
spellingShingle Production technology for entomopathogenic fungus using a biphasic culture system
Machado, Ana Carolina Ribeiro
Lecanicillium lecanii; conidial viability; liquid media; mass production; solid media
Lecanicillium lecanii; viabilidade de conídios; meios líquidos; produção massal; meios sólidos
title_short Production technology for entomopathogenic fungus using a biphasic culture system
title_full Production technology for entomopathogenic fungus using a biphasic culture system
title_fullStr Production technology for entomopathogenic fungus using a biphasic culture system
title_full_unstemmed Production technology for entomopathogenic fungus using a biphasic culture system
title_sort Production technology for entomopathogenic fungus using a biphasic culture system
author Machado, Ana Carolina Ribeiro
author_facet Machado, Ana Carolina Ribeiro
Monteiro, Antonio Carlos
de Almeida, Aline Maria Belasco
Geraldo Martins, Maria Inez Espagnoli
author_role author
author2 Monteiro, Antonio Carlos
de Almeida, Aline Maria Belasco
Geraldo Martins, Maria Inez Espagnoli
author2_role author
author
author
dc.contributor.none.fl_str_mv
CAPES
dc.contributor.author.fl_str_mv Machado, Ana Carolina Ribeiro
Monteiro, Antonio Carlos
de Almeida, Aline Maria Belasco
Geraldo Martins, Maria Inez Espagnoli
dc.subject.por.fl_str_mv Lecanicillium lecanii; conidial viability; liquid media; mass production; solid media
Lecanicillium lecanii; viabilidade de conídios; meios líquidos; produção massal; meios sólidos
topic Lecanicillium lecanii; conidial viability; liquid media; mass production; solid media
Lecanicillium lecanii; viabilidade de conídios; meios líquidos; produção massal; meios sólidos
description The objective of this research was to evaluate combinations of liquid media obtained from agro-industrial residues and by-products, with solid media prepared with mixtures of grains and their derivatives, aiming to increase the production of JAB 02 and JAB 45 isolates of Lecanicillium lecanii. Sporulation, conidial viability and process yield were evaluated as well as the production costs using the JAB 45 isolate as a model system were analyzed. The production of JAB 02 was not increased using the biphasic culture. For JAB 45, some combinations provided an increase in yield, especially cheese whey with wheat bran and wheat grain, with lower production costs. Viability was not influenced by the production method, and the combinations showed no differences in the process yield. The biphasic method is suitable for the production of L. lecanii, and proves to be an appropriate technology to use in mass production by biofactories.
publishDate 2011
dc.date.none.fl_str_mv 2011-01-27
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/8575
url https://seer.sct.embrapa.br/index.php/pab/article/view/8575
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/8575/6126
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/8575/4297
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.45, n.10, out. 2010; 1157-1163
Pesquisa Agropecuária Brasileira; v.45, n.10, out. 2010; 1157-1163
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
_version_ 1793416651449827328

Registros relacionados