Isoenzymatic activity in wheat plants infected by virus SBWMV

Detalhes bibliográficos
Autor(a) principal: de Souza, Rocheli
Data de Publicação: 2005
Outros Autores: Schons, Jurema, Brammer, Sandra Patussi, Prestes, Ariano Moraes, Scheeren, Pedro Luiz, e Silva, Marcio Só, Del Duca, Leo de Jesus Antunes
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/7035
Resumo: The aim of this work was to elucidate the effect of the Soil-borne wheat mosaic virus (SBWMV) on the activity and on the isoenzymatic expression of esterases, peroxidases, and aspartate aminotranferases in wheat plants. Biochemical analyses were carried out for four cultivars and one line of wheat, 45 days after emergence with different levels of resistance to SBWMV: BRS Guabiju, BRS 194, BRS 179, BR 23 and PF 980524. In general, intra and interpopulation differences in quality and quantity were detected, when comparing plants with and without symptoms of SBWMV. Nine band patterns were determined in both situations for esterase. To peroxidase and aspartato aminotransferase, three band patterns were detected for both conditions. Eletrophoretic patterns of SBWMV infected plants were observed, when compared with noninfected ones, notably for esterase, which provides a more precise identification of cell metabolic condition.
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spelling Isoenzymatic activity in wheat plants infected by virus SBWMVAtividade isoenzimática em plantas de trigo infectadas com o vírus SBWMVTriticum aestivum; genetic variability; esterase; peroxidase; aspartate aminotransferaseTriticum aestivum; variabilidade genética; esterase; peroxidase; aspartato aminotransferase The aim of this work was to elucidate the effect of the Soil-borne wheat mosaic virus (SBWMV) on the activity and on the isoenzymatic expression of esterases, peroxidases, and aspartate aminotranferases in wheat plants. Biochemical analyses were carried out for four cultivars and one line of wheat, 45 days after emergence with different levels of resistance to SBWMV: BRS Guabiju, BRS 194, BRS 179, BR 23 and PF 980524. In general, intra and interpopulation differences in quality and quantity were detected, when comparing plants with and without symptoms of SBWMV. Nine band patterns were determined in both situations for esterase. To peroxidase and aspartato aminotransferase, three band patterns were detected for both conditions. Eletrophoretic patterns of SBWMV infected plants were observed, when compared with noninfected ones, notably for esterase, which provides a more precise identification of cell metabolic condition.O objetivo deste trabalho foi elucidar a atividade e a expressão isoenzimática das esterases, das peroxidases e das aspartato aminotransferases em função da infecção de plantas de trigo pelo Soil-borne wheat mosaic virus (SBWMV). Foram analisadas, aos 45 dias após a emergência, quatro cultivares e uma linhagem de trigo, com diferentes níveis de resistência ao SBWMV: BRS Guabiju, BRS 194, BRS 179, BR 23 e PF 980524. De modo geral, ocorreram diferenças qualitativas e quantitativas intra e interpopulacional, quando comparadas plantas assintomáticas e sintomáticas ao SBWMV. Para o sistema esterase, nove padrões de bandas foram determinados e para peroxidase e aspartato aminotransferase foram detectados três padrões de bandas, para ambas as condições. Padrões eletroforéticos foram observados para plantas infectadas, quando comparadas com as não infectadas, destacando-se a atividade da esterase, o que permitiu identificar com maior precisão o estado metabólico e diferenciado das células.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária Brasileirade Souza, RocheliSchons, JuremaBrammer, Sandra PatussiPrestes, Ariano MoraesScheeren, Pedro Luize Silva, Marcio SóDel Duca, Leo de Jesus Antunes2005-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/7035Pesquisa Agropecuaria Brasileira; v.40, n.9, set. 2005; 845-852Pesquisa Agropecuária Brasileira; v.40, n.9, set. 2005; 845-8521678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/7035/4083info:eu-repo/semantics/openAccess2014-09-19T19:35:38Zoai:ojs.seer.sct.embrapa.br:article/7035Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-09-19T19:35:38Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Isoenzymatic activity in wheat plants infected by virus SBWMV
Atividade isoenzimática em plantas de trigo infectadas com o vírus SBWMV
title Isoenzymatic activity in wheat plants infected by virus SBWMV
spellingShingle Isoenzymatic activity in wheat plants infected by virus SBWMV
de Souza, Rocheli
Triticum aestivum; genetic variability; esterase; peroxidase; aspartate aminotransferase
Triticum aestivum; variabilidade genética; esterase; peroxidase; aspartato aminotransferase
title_short Isoenzymatic activity in wheat plants infected by virus SBWMV
title_full Isoenzymatic activity in wheat plants infected by virus SBWMV
title_fullStr Isoenzymatic activity in wheat plants infected by virus SBWMV
title_full_unstemmed Isoenzymatic activity in wheat plants infected by virus SBWMV
title_sort Isoenzymatic activity in wheat plants infected by virus SBWMV
author de Souza, Rocheli
author_facet de Souza, Rocheli
Schons, Jurema
Brammer, Sandra Patussi
Prestes, Ariano Moraes
Scheeren, Pedro Luiz
e Silva, Marcio Só
Del Duca, Leo de Jesus Antunes
author_role author
author2 Schons, Jurema
Brammer, Sandra Patussi
Prestes, Ariano Moraes
Scheeren, Pedro Luiz
e Silva, Marcio Só
Del Duca, Leo de Jesus Antunes
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv de Souza, Rocheli
Schons, Jurema
Brammer, Sandra Patussi
Prestes, Ariano Moraes
Scheeren, Pedro Luiz
e Silva, Marcio Só
Del Duca, Leo de Jesus Antunes
dc.subject.por.fl_str_mv Triticum aestivum; genetic variability; esterase; peroxidase; aspartate aminotransferase
Triticum aestivum; variabilidade genética; esterase; peroxidase; aspartato aminotransferase
topic Triticum aestivum; genetic variability; esterase; peroxidase; aspartate aminotransferase
Triticum aestivum; variabilidade genética; esterase; peroxidase; aspartato aminotransferase
description The aim of this work was to elucidate the effect of the Soil-borne wheat mosaic virus (SBWMV) on the activity and on the isoenzymatic expression of esterases, peroxidases, and aspartate aminotranferases in wheat plants. Biochemical analyses were carried out for four cultivars and one line of wheat, 45 days after emergence with different levels of resistance to SBWMV: BRS Guabiju, BRS 194, BRS 179, BR 23 and PF 980524. In general, intra and interpopulation differences in quality and quantity were detected, when comparing plants with and without symptoms of SBWMV. Nine band patterns were determined in both situations for esterase. To peroxidase and aspartato aminotransferase, three band patterns were detected for both conditions. Eletrophoretic patterns of SBWMV infected plants were observed, when compared with noninfected ones, notably for esterase, which provides a more precise identification of cell metabolic condition.
publishDate 2005
dc.date.none.fl_str_mv 2005-09-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/7035
url https://seer.sct.embrapa.br/index.php/pab/article/view/7035
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/7035/4083
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.40, n.9, set. 2005; 845-852
Pesquisa Agropecuária Brasileira; v.40, n.9, set. 2005; 845-852
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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