Androgenic response of Brazilian wheat genotypes to different pretreatments of spikes and to a gelling agent
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Agropecuária Brasileira (Online) |
Texto Completo: | https://seer.sct.embrapa.br/index.php/pab/article/view/21849 |
Resumo: | The objective of this work was to analyze the androgenic response of Brazilian wheat genotypes to different pretreatments of the spikes, prior to the culture of isolated microspores, and to the effect of a gelling agent in the induction culture medium. Five genotypes were evaluated for embryo formation, green plantregeneration, and spontaneous chromosome duplication. Wheat spikes were subjected to two pretreatments:cold, at 4°C for 21 days; and 2-hydroxynicotinic acid, at 32°C for two days. Culture media were evaluated with or without Ficoll as a gelling agent. Cold produced more embryos and green plants than the chemical pretreatment in four out of five genotypes. Only two genotypes treated with 2-hydroxynicotinic acid were able to produce plants, and one of them produced a single albino plant. Medium containing Ficoll produced moreembryos than liquid medium and promoted a higher number of plants. Spontaneous chromosome duplication varies between genotypes and pretreatments, and shows high variability. |
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Androgenic response of Brazilian wheat genotypes to different pretreatments of spikes and to a gelling agentResposta androgênica de genótipos brasileiros de trigo a diferentes pré-tratamentos das espigas e a um agente gelificanteTriticum aestivum; albinism; androgenesis; doubled haploid; isolated microspore culture; recalcitranceTriticum aestivum; albinismo; androgênese; duplo-haploide; cultura de micrósporos isolados; recalcitrânciaThe objective of this work was to analyze the androgenic response of Brazilian wheat genotypes to different pretreatments of the spikes, prior to the culture of isolated microspores, and to the effect of a gelling agent in the induction culture medium. Five genotypes were evaluated for embryo formation, green plantregeneration, and spontaneous chromosome duplication. Wheat spikes were subjected to two pretreatments:cold, at 4°C for 21 days; and 2-hydroxynicotinic acid, at 32°C for two days. Culture media were evaluated with or without Ficoll as a gelling agent. Cold produced more embryos and green plants than the chemical pretreatment in four out of five genotypes. Only two genotypes treated with 2-hydroxynicotinic acid were able to produce plants, and one of them produced a single albino plant. Medium containing Ficoll produced moreembryos than liquid medium and promoted a higher number of plants. Spontaneous chromosome duplication varies between genotypes and pretreatments, and shows high variability.O objetivo deste trabalho foi analisar a resposta androgênica de genótipos brasileiros de trigo a diferentes pré-tratamentos das espigas, antes da cultura de micrósporos isolados, e ao efeito de um agente gelificante no meio de cultura de indução. Cinco genótipos foram avaliados quanto à formação de embriões, regeneração de plantas verdes e à duplicação espontânea dos cromossomos. Espigas de trigo foram submetidas a dois pré-tratamentos: frio, a 4°C por 21 dias; e ácido 2-hidroxinicotínico, a 32°C por dois dias. Os meios decultura foram avaliados com ou sem Ficoll como agente gelificante. O frio produziu mais embriões e plantas verdes do que o pré-tratamento químico, em quatro dos cinco genótipos testados. Apenas dois genótipos tratados com ácido 2-hidroxinicotínico foram capazes de produzir plantas, e um deles produziu uma única planta albina. O meio com Ficoll produziu mais embriões do que o meio líquido e gerou maior número de plantas. A duplicação espontânea dos cromossomos varia entre os genótipos e os pré-tratamentos e apresentaalta variabilidade.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraCAPES, EMBRAPA TRIGOPoersch-Bortolon, Liane BalvediScagliusi, Sandra Maria MansurYamazaki-Lau, EleneBodanese-Zanettini, Maria Helena2016-12-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/21849Pesquisa Agropecuaria Brasileira; v.51, n.11, nov. 2016; 1839-1847Pesquisa Agropecuária Brasileira; v.51, n.11, nov. 2016; 1839-18471678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/21849/13630https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/21849/13934https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/21849/13935Direitos autorais 2016 Pesquisa Agropecuária Brasileirainfo:eu-repo/semantics/openAccess2017-01-18T12:16:38Zoai:ojs.seer.sct.embrapa.br:article/21849Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2017-01-18T12:16:38Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Androgenic response of Brazilian wheat genotypes to different pretreatments of spikes and to a gelling agent Resposta androgênica de genótipos brasileiros de trigo a diferentes pré-tratamentos das espigas e a um agente gelificante |
title |
Androgenic response of Brazilian wheat genotypes to different pretreatments of spikes and to a gelling agent |
spellingShingle |
Androgenic response of Brazilian wheat genotypes to different pretreatments of spikes and to a gelling agent Poersch-Bortolon, Liane Balvedi Triticum aestivum; albinism; androgenesis; doubled haploid; isolated microspore culture; recalcitrance Triticum aestivum; albinismo; androgênese; duplo-haploide; cultura de micrósporos isolados; recalcitrância |
title_short |
Androgenic response of Brazilian wheat genotypes to different pretreatments of spikes and to a gelling agent |
title_full |
Androgenic response of Brazilian wheat genotypes to different pretreatments of spikes and to a gelling agent |
title_fullStr |
Androgenic response of Brazilian wheat genotypes to different pretreatments of spikes and to a gelling agent |
title_full_unstemmed |
Androgenic response of Brazilian wheat genotypes to different pretreatments of spikes and to a gelling agent |
title_sort |
Androgenic response of Brazilian wheat genotypes to different pretreatments of spikes and to a gelling agent |
author |
Poersch-Bortolon, Liane Balvedi |
author_facet |
Poersch-Bortolon, Liane Balvedi Scagliusi, Sandra Maria Mansur Yamazaki-Lau, Elene Bodanese-Zanettini, Maria Helena |
author_role |
author |
author2 |
Scagliusi, Sandra Maria Mansur Yamazaki-Lau, Elene Bodanese-Zanettini, Maria Helena |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
CAPES, EMBRAPA TRIGO |
dc.contributor.author.fl_str_mv |
Poersch-Bortolon, Liane Balvedi Scagliusi, Sandra Maria Mansur Yamazaki-Lau, Elene Bodanese-Zanettini, Maria Helena |
dc.subject.por.fl_str_mv |
Triticum aestivum; albinism; androgenesis; doubled haploid; isolated microspore culture; recalcitrance Triticum aestivum; albinismo; androgênese; duplo-haploide; cultura de micrósporos isolados; recalcitrância |
topic |
Triticum aestivum; albinism; androgenesis; doubled haploid; isolated microspore culture; recalcitrance Triticum aestivum; albinismo; androgênese; duplo-haploide; cultura de micrósporos isolados; recalcitrância |
description |
The objective of this work was to analyze the androgenic response of Brazilian wheat genotypes to different pretreatments of the spikes, prior to the culture of isolated microspores, and to the effect of a gelling agent in the induction culture medium. Five genotypes were evaluated for embryo formation, green plantregeneration, and spontaneous chromosome duplication. Wheat spikes were subjected to two pretreatments:cold, at 4°C for 21 days; and 2-hydroxynicotinic acid, at 32°C for two days. Culture media were evaluated with or without Ficoll as a gelling agent. Cold produced more embryos and green plants than the chemical pretreatment in four out of five genotypes. Only two genotypes treated with 2-hydroxynicotinic acid were able to produce plants, and one of them produced a single albino plant. Medium containing Ficoll produced moreembryos than liquid medium and promoted a higher number of plants. Spontaneous chromosome duplication varies between genotypes and pretreatments, and shows high variability. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-12-23 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/21849 |
url |
https://seer.sct.embrapa.br/index.php/pab/article/view/21849 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/21849/13630 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/21849/13934 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/21849/13935 |
dc.rights.driver.fl_str_mv |
Direitos autorais 2016 Pesquisa Agropecuária Brasileira info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Direitos autorais 2016 Pesquisa Agropecuária Brasileira |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
dc.source.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira; v.51, n.11, nov. 2016; 1839-1847 Pesquisa Agropecuária Brasileira; v.51, n.11, nov. 2016; 1839-1847 1678-3921 0100-104x reponame:Pesquisa Agropecuária Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Agropecuária Brasileira (Online) |
collection |
Pesquisa Agropecuária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
pab@sct.embrapa.br || sct.pab@embrapa.br |
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1793416657507450880 |