Androgenic response of Brazilian wheat genotypes to different pretreatments of spikes and to a gelling agent

Detalhes bibliográficos
Autor(a) principal: Poersch-Bortolon, Liane Balvedi
Data de Publicação: 2016
Outros Autores: Scagliusi, Sandra Maria Mansur, Yamazaki-Lau, Elene, Bodanese-Zanettini, Maria Helena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/21849
Resumo: The objective of this work was to analyze the androgenic response of Brazilian wheat genotypes to different pretreatments of the spikes, prior to the culture of isolated microspores, and to the effect of a gelling agent in the induction culture medium. Five genotypes were evaluated for embryo formation, green plantregeneration, and spontaneous chromosome duplication. Wheat spikes were subjected to two pretreatments:cold, at 4°C for 21 days; and 2-hydroxynicotinic acid, at 32°C for two days. Culture media were evaluated with or without Ficoll as a gelling agent. Cold produced more embryos and green plants than the chemical pretreatment in four out of five genotypes. Only two genotypes treated with 2-hydroxynicotinic acid were able to produce plants, and one of them produced a single albino plant. Medium containing Ficoll produced moreembryos than liquid medium and promoted a higher number of plants. Spontaneous chromosome duplication varies between genotypes and pretreatments, and shows high variability.
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spelling Androgenic response of Brazilian wheat genotypes to different pretreatments of spikes and to a gelling agentResposta androgênica de genótipos brasileiros de trigo a diferentes pré-tratamentos das espigas e a um agente gelificanteTriticum aestivum; albinism; androgenesis; doubled haploid; isolated microspore culture; recalcitranceTriticum aestivum; albinismo; androgênese; duplo-haploide; cultura de micrósporos isolados; recalcitrânciaThe objective of this work was to analyze the androgenic response of Brazilian wheat genotypes to different pretreatments of the spikes, prior to the culture of isolated microspores, and to the effect of a gelling agent in the induction culture medium. Five genotypes were evaluated for embryo formation, green plantregeneration, and spontaneous chromosome duplication. Wheat spikes were subjected to two pretreatments:cold, at 4°C for 21 days; and 2-hydroxynicotinic acid, at 32°C for two days. Culture media were evaluated with or without Ficoll as a gelling agent. Cold produced more embryos and green plants than the chemical pretreatment in four out of five genotypes. Only two genotypes treated with 2-hydroxynicotinic acid were able to produce plants, and one of them produced a single albino plant. Medium containing Ficoll produced moreembryos than liquid medium and promoted a higher number of plants. Spontaneous chromosome duplication varies between genotypes and pretreatments, and shows high variability.O objetivo deste trabalho foi analisar a resposta androgênica de genótipos brasileiros de trigo a diferentes pré-tratamentos das espigas, antes da cultura de micrósporos isolados, e ao efeito de um agente gelificante no meio de cultura de indução. Cinco genótipos foram avaliados quanto à formação de embriões, regeneração de plantas verdes e à duplicação espontânea dos cromossomos. Espigas de trigo foram submetidas a dois pré-tratamentos: frio, a 4°C por 21 dias; e ácido 2-hidroxinicotínico, a 32°C por dois dias. Os meios decultura foram avaliados com ou sem Ficoll como agente gelificante. O frio produziu mais embriões e plantas verdes do que o pré-tratamento químico, em quatro dos cinco genótipos testados. Apenas dois genótipos tratados com ácido 2-hidroxinicotínico foram capazes de produzir plantas, e um deles produziu uma única planta albina. O meio com Ficoll produziu mais embriões do que o meio líquido e gerou maior número de plantas. A duplicação espontânea dos cromossomos varia entre os genótipos e os pré-tratamentos e apresentaalta variabilidade.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraCAPES, EMBRAPA TRIGOPoersch-Bortolon, Liane BalvediScagliusi, Sandra Maria MansurYamazaki-Lau, EleneBodanese-Zanettini, Maria Helena2016-12-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/21849Pesquisa Agropecuaria Brasileira; v.51, n.11, nov. 2016; 1839-1847Pesquisa Agropecuária Brasileira; v.51, n.11, nov. 2016; 1839-18471678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/21849/13630https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/21849/13934https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/21849/13935Direitos autorais 2016 Pesquisa Agropecuária Brasileirainfo:eu-repo/semantics/openAccess2017-01-18T12:16:38Zoai:ojs.seer.sct.embrapa.br:article/21849Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2017-01-18T12:16:38Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Androgenic response of Brazilian wheat genotypes to different pretreatments of spikes and to a gelling agent
Resposta androgênica de genótipos brasileiros de trigo a diferentes pré-tratamentos das espigas e a um agente gelificante
title Androgenic response of Brazilian wheat genotypes to different pretreatments of spikes and to a gelling agent
spellingShingle Androgenic response of Brazilian wheat genotypes to different pretreatments of spikes and to a gelling agent
Poersch-Bortolon, Liane Balvedi
Triticum aestivum; albinism; androgenesis; doubled haploid; isolated microspore culture; recalcitrance
Triticum aestivum; albinismo; androgênese; duplo-haploide; cultura de micrósporos isolados; recalcitrância
title_short Androgenic response of Brazilian wheat genotypes to different pretreatments of spikes and to a gelling agent
title_full Androgenic response of Brazilian wheat genotypes to different pretreatments of spikes and to a gelling agent
title_fullStr Androgenic response of Brazilian wheat genotypes to different pretreatments of spikes and to a gelling agent
title_full_unstemmed Androgenic response of Brazilian wheat genotypes to different pretreatments of spikes and to a gelling agent
title_sort Androgenic response of Brazilian wheat genotypes to different pretreatments of spikes and to a gelling agent
author Poersch-Bortolon, Liane Balvedi
author_facet Poersch-Bortolon, Liane Balvedi
Scagliusi, Sandra Maria Mansur
Yamazaki-Lau, Elene
Bodanese-Zanettini, Maria Helena
author_role author
author2 Scagliusi, Sandra Maria Mansur
Yamazaki-Lau, Elene
Bodanese-Zanettini, Maria Helena
author2_role author
author
author
dc.contributor.none.fl_str_mv CAPES, EMBRAPA TRIGO
dc.contributor.author.fl_str_mv Poersch-Bortolon, Liane Balvedi
Scagliusi, Sandra Maria Mansur
Yamazaki-Lau, Elene
Bodanese-Zanettini, Maria Helena
dc.subject.por.fl_str_mv Triticum aestivum; albinism; androgenesis; doubled haploid; isolated microspore culture; recalcitrance
Triticum aestivum; albinismo; androgênese; duplo-haploide; cultura de micrósporos isolados; recalcitrância
topic Triticum aestivum; albinism; androgenesis; doubled haploid; isolated microspore culture; recalcitrance
Triticum aestivum; albinismo; androgênese; duplo-haploide; cultura de micrósporos isolados; recalcitrância
description The objective of this work was to analyze the androgenic response of Brazilian wheat genotypes to different pretreatments of the spikes, prior to the culture of isolated microspores, and to the effect of a gelling agent in the induction culture medium. Five genotypes were evaluated for embryo formation, green plantregeneration, and spontaneous chromosome duplication. Wheat spikes were subjected to two pretreatments:cold, at 4°C for 21 days; and 2-hydroxynicotinic acid, at 32°C for two days. Culture media were evaluated with or without Ficoll as a gelling agent. Cold produced more embryos and green plants than the chemical pretreatment in four out of five genotypes. Only two genotypes treated with 2-hydroxynicotinic acid were able to produce plants, and one of them produced a single albino plant. Medium containing Ficoll produced moreembryos than liquid medium and promoted a higher number of plants. Spontaneous chromosome duplication varies between genotypes and pretreatments, and shows high variability.
publishDate 2016
dc.date.none.fl_str_mv 2016-12-23
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/21849
url https://seer.sct.embrapa.br/index.php/pab/article/view/21849
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/21849/13630
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/21849/13934
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/21849/13935
dc.rights.driver.fl_str_mv Direitos autorais 2016 Pesquisa Agropecuária Brasileira
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Direitos autorais 2016 Pesquisa Agropecuária Brasileira
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.51, n.11, nov. 2016; 1839-1847
Pesquisa Agropecuária Brasileira; v.51, n.11, nov. 2016; 1839-1847
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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