Subunit composition of seed storage proteins in high-protein soybean genotypes
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Agropecuária Brasileira (Online) |
Texto Completo: | https://seer.sct.embrapa.br/index.php/pab/article/view/8260 |
Resumo: | The objective of this work was to quantify the accumulation of the major seed storage protein subunits, β-conglycinin and glycinin, and how they influence yield and protein and oil contents in high-protein soybean genotypes. The relative accumulation of subunits was calculated by scanning SDS-PAGE gels using densitometry. The protein content of the tested genotypes was higher than control cultivar in the same maturity group. Several genotypes with improved protein content and with unchanged yield or oil content were developed as a result of new breeding initiatives. This research confirmed that high-protein cultivars accumulate higher amounts of glycinin and β-conglycinin. Genotypes KO5427, KO5428, and KO5429, which accumulated lower quantities of all subunits of glycinin and β-conglycinin, were the only exceptions. Attention should be given to genotypes KO5314 and KO5317, which accumulated significantly higher amounts of both subunits of glycinin, and to genotypes KO5425, KO5319, KO539 and KO536, which accumulated significantly higher amounts of β-conglycinin subunits. These findings suggest that some of the tested genotypes could be beneficial in different breeding programs aimed at the production of agronomically viable plants, yielding high-protein seed with specific composition of storage proteins for specific food applications. |
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Subunit composition of seed storage proteins in high-protein soybean genotypesComposição de subunidades de proteínas de reserva em genótipos de soja com alto teor de proteínaGlycine max; β-conglycinin; glycinin; protein content; subunit compositionGlycine max; β-conglicinina; glicinina; conteúdo de proteína; composição das subunidadesThe objective of this work was to quantify the accumulation of the major seed storage protein subunits, β-conglycinin and glycinin, and how they influence yield and protein and oil contents in high-protein soybean genotypes. The relative accumulation of subunits was calculated by scanning SDS-PAGE gels using densitometry. The protein content of the tested genotypes was higher than control cultivar in the same maturity group. Several genotypes with improved protein content and with unchanged yield or oil content were developed as a result of new breeding initiatives. This research confirmed that high-protein cultivars accumulate higher amounts of glycinin and β-conglycinin. Genotypes KO5427, KO5428, and KO5429, which accumulated lower quantities of all subunits of glycinin and β-conglycinin, were the only exceptions. Attention should be given to genotypes KO5314 and KO5317, which accumulated significantly higher amounts of both subunits of glycinin, and to genotypes KO5425, KO5319, KO539 and KO536, which accumulated significantly higher amounts of β-conglycinin subunits. These findings suggest that some of the tested genotypes could be beneficial in different breeding programs aimed at the production of agronomically viable plants, yielding high-protein seed with specific composition of storage proteins for specific food applications.O objetivo deste trabalho foi quantificar o acúmulo das principais subunidades de proteínas de reserva da soja, β-conglicinina e glicinina, e como elas influenciam a produtividade e os conteúdos de proteína e de óleo em genótipos de soja com alto conteúdo de proteína. A acumulação relativa de subunidades foi calculada por escaneamento em géis SDS-PAGE, com uso de densitometria. O conteúdo de proteínas dos genótipos testados foi maior que o da cultivar controle dentro do mesmo grupo de maturação. Vários genótipos com conteúdo de proteína aumentado, mas com produtividade ou conteúdo de óleo inalterados, foram desenvolvidos como resultados de novas iniciativas de melhoramento. Esta pesquisa confirmou que as cultivares com alto conteúdo de proteína acumulam maior quantidade de glicinina e â-conglicinina. Os genótipos KO5427, KO5428, e KO5429, que acumularam menor quantidade de todas as subunidades de glicinina e β-conglicinina, foram as únicas exceções. Deve-se atentar para os genótipos KO5314 e KO5317, que acumularam quantidades significativamente maiores das duas subunidades da glicinina, e para os genótipos KO5425, KO539 e KO536, que acumularam quantidades significativamente maiores das subunidades de β-conglicinina. Estes resultados indicam que alguns dos genótipos testados poderiam ser benéficos em programas de melhoramento que visem à produção de plantas agronomicamente viáveis, com sementes com alto conteúdo de proteínas e composição específica de proteínas de reserva para fins alimentícios definidos.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraTaski-Ajdukovic, KsenijaDjordjevic, VukVidic, MilosVujakovic, Milka2011-01-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/8260Pesquisa Agropecuaria Brasileira; v.45, n.7, jul. 2010; 721-729Pesquisa Agropecuária Brasileira; v.45, n.7, jul. 2010; 721-7291678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/8260/6048https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/8260/3925https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/8260/3926info:eu-repo/semantics/openAccess2014-11-04T16:53:13Zoai:ojs.seer.sct.embrapa.br:article/8260Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-11-04T16:53:13Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Subunit composition of seed storage proteins in high-protein soybean genotypes Composição de subunidades de proteínas de reserva em genótipos de soja com alto teor de proteína |
title |
Subunit composition of seed storage proteins in high-protein soybean genotypes |
spellingShingle |
Subunit composition of seed storage proteins in high-protein soybean genotypes Taski-Ajdukovic, Ksenija Glycine max; β-conglycinin; glycinin; protein content; subunit composition Glycine max; β-conglicinina; glicinina; conteúdo de proteína; composição das subunidades |
title_short |
Subunit composition of seed storage proteins in high-protein soybean genotypes |
title_full |
Subunit composition of seed storage proteins in high-protein soybean genotypes |
title_fullStr |
Subunit composition of seed storage proteins in high-protein soybean genotypes |
title_full_unstemmed |
Subunit composition of seed storage proteins in high-protein soybean genotypes |
title_sort |
Subunit composition of seed storage proteins in high-protein soybean genotypes |
author |
Taski-Ajdukovic, Ksenija |
author_facet |
Taski-Ajdukovic, Ksenija Djordjevic, Vuk Vidic, Milos Vujakovic, Milka |
author_role |
author |
author2 |
Djordjevic, Vuk Vidic, Milos Vujakovic, Milka |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
|
dc.contributor.author.fl_str_mv |
Taski-Ajdukovic, Ksenija Djordjevic, Vuk Vidic, Milos Vujakovic, Milka |
dc.subject.por.fl_str_mv |
Glycine max; β-conglycinin; glycinin; protein content; subunit composition Glycine max; β-conglicinina; glicinina; conteúdo de proteína; composição das subunidades |
topic |
Glycine max; β-conglycinin; glycinin; protein content; subunit composition Glycine max; β-conglicinina; glicinina; conteúdo de proteína; composição das subunidades |
description |
The objective of this work was to quantify the accumulation of the major seed storage protein subunits, β-conglycinin and glycinin, and how they influence yield and protein and oil contents in high-protein soybean genotypes. The relative accumulation of subunits was calculated by scanning SDS-PAGE gels using densitometry. The protein content of the tested genotypes was higher than control cultivar in the same maturity group. Several genotypes with improved protein content and with unchanged yield or oil content were developed as a result of new breeding initiatives. This research confirmed that high-protein cultivars accumulate higher amounts of glycinin and β-conglycinin. Genotypes KO5427, KO5428, and KO5429, which accumulated lower quantities of all subunits of glycinin and β-conglycinin, were the only exceptions. Attention should be given to genotypes KO5314 and KO5317, which accumulated significantly higher amounts of both subunits of glycinin, and to genotypes KO5425, KO5319, KO539 and KO536, which accumulated significantly higher amounts of β-conglycinin subunits. These findings suggest that some of the tested genotypes could be beneficial in different breeding programs aimed at the production of agronomically viable plants, yielding high-protein seed with specific composition of storage proteins for specific food applications. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-01-26 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/8260 |
url |
https://seer.sct.embrapa.br/index.php/pab/article/view/8260 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/8260/6048 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/8260/3925 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/8260/3926 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
dc.source.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira; v.45, n.7, jul. 2010; 721-729 Pesquisa Agropecuária Brasileira; v.45, n.7, jul. 2010; 721-729 1678-3921 0100-104x reponame:Pesquisa Agropecuária Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Agropecuária Brasileira (Online) |
collection |
Pesquisa Agropecuária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
pab@sct.embrapa.br || sct.pab@embrapa.br |
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1793416659889815552 |