"RFLP" analysis of aluminum tolerance in chromosome 2 in maize

Detalhes bibliográficos
Autor(a) principal: Brondani, Claudio
Data de Publicação: 1996
Outros Autores: Paiva, Edilson
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/4523
Resumo: This paper aimed to look for genomic regions related with the aluminum tolerance in chromosome 2 of maize using the RFLP technique. Four aluminum tolerant maize lines (15, 16, 699 and 1327) and three non-tolerant ones (19, 53 and 57) were crossed: 57 x 1327, 57 x 699, 53 x 16, 53 x 15 and 19 x 1327. The degree of tolerance to aluminum of the parents, F1's and F2 were determined through measurements of the Relative Seminal Root Length of seedlings grown in nutrient solution containing 222 µmol of Al/L. The RFLP analysis was made in the 6 more tolerant F2 plants and in the 6 more susceptibles plants of each cross, testing two restriction enzymes and five probes from different regions of chromosome 2 of maize, which have loci to Al tolerance. Tolerant and susceptible RFLP different markers were identified to each cross. These markers are potentially useful in marker assisted selection with RFLP's in the genetic breeding for Al tolerance in maize.
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spelling "RFLP" analysis of aluminum tolerance in chromosome 2 in maizeAnálise de "RFLP" da tolerância à toxidez do alumínio no cromossomo 2 do milhoacid soils; Zea mays; DNA probes; nutritive solution; restriction enzymessolos ácidos; Zea mays; sondas de DNA; solução nutritiva; enzimas de restriçãoThis paper aimed to look for genomic regions related with the aluminum tolerance in chromosome 2 of maize using the RFLP technique. Four aluminum tolerant maize lines (15, 16, 699 and 1327) and three non-tolerant ones (19, 53 and 57) were crossed: 57 x 1327, 57 x 699, 53 x 16, 53 x 15 and 19 x 1327. The degree of tolerance to aluminum of the parents, F1's and F2 were determined through measurements of the Relative Seminal Root Length of seedlings grown in nutrient solution containing 222 µmol of Al/L. The RFLP analysis was made in the 6 more tolerant F2 plants and in the 6 more susceptibles plants of each cross, testing two restriction enzymes and five probes from different regions of chromosome 2 of maize, which have loci to Al tolerance. Tolerant and susceptible RFLP different markers were identified to each cross. These markers are potentially useful in marker assisted selection with RFLP's in the genetic breeding for Al tolerance in maize.O presente trabalho objetivou, via técnica de RFLP, localizar, no cromossomo 2 do milho, regiões genômicas relacionadas com a resistência ao alumínio tóxico, o qual encontra-se presente em cerca de 60% do território brasileiro. Linhagens de milho tolerantes (15, 16, 699 e 1327) e suscetíveis (19, 53 e 57) ao alumínio, foram utilizadas nos cruzamentos 57 x 1.327, 57 x 699, 53 x 16, 53 x 15 e 19 x 1.327. Avaliaram-se a resistência ao alumínio dos parentais, dos F1 's e de 300 indivíduos F2 de cada cruzamento em solução nutritiva contendo 222 µmol Al/L, medindo-se o Comprimento Relativo da Raiz Seminal como marcador morfológico. A análise de RFLP foi efetuada nas seis plantas mais tolerantes e nas seis mais suscetíveis ao A1, em cada cruzamento, testando-se as combinações de duas enzimas de restrição e cinco sondas mapeadas em diferentes regiões do cromossomo 2 do milho. Foram identificados marcadores relacionados com a tolerância ou suscetibilidade ao alumínio específicos para cada cruzamento. Tais marcadores têm uso potencial para a seleção assistida por RFLP's no melhoramento genético visando à tolerância ao alumínio tóxico em milho.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraBrondani, ClaudioPaiva, Edilson1996-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/4523Pesquisa Agropecuaria Brasileira; v.31, n.8, ago. 1996; 575-579Pesquisa Agropecuária Brasileira; v.31, n.8, ago. 1996; 575-5791678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/4523/1809info:eu-repo/semantics/openAccess2015-09-24T12:27:43Zoai:ojs.seer.sct.embrapa.br:article/4523Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2015-09-24T12:27:43Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv "RFLP" analysis of aluminum tolerance in chromosome 2 in maize
Análise de "RFLP" da tolerância à toxidez do alumínio no cromossomo 2 do milho
title "RFLP" analysis of aluminum tolerance in chromosome 2 in maize
spellingShingle "RFLP" analysis of aluminum tolerance in chromosome 2 in maize
Brondani, Claudio
acid soils; Zea mays; DNA probes; nutritive solution; restriction enzymes
solos ácidos; Zea mays; sondas de DNA; solução nutritiva; enzimas de restrição
title_short "RFLP" analysis of aluminum tolerance in chromosome 2 in maize
title_full "RFLP" analysis of aluminum tolerance in chromosome 2 in maize
title_fullStr "RFLP" analysis of aluminum tolerance in chromosome 2 in maize
title_full_unstemmed "RFLP" analysis of aluminum tolerance in chromosome 2 in maize
title_sort "RFLP" analysis of aluminum tolerance in chromosome 2 in maize
author Brondani, Claudio
author_facet Brondani, Claudio
Paiva, Edilson
author_role author
author2 Paiva, Edilson
author2_role author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Brondani, Claudio
Paiva, Edilson
dc.subject.por.fl_str_mv acid soils; Zea mays; DNA probes; nutritive solution; restriction enzymes
solos ácidos; Zea mays; sondas de DNA; solução nutritiva; enzimas de restrição
topic acid soils; Zea mays; DNA probes; nutritive solution; restriction enzymes
solos ácidos; Zea mays; sondas de DNA; solução nutritiva; enzimas de restrição
description This paper aimed to look for genomic regions related with the aluminum tolerance in chromosome 2 of maize using the RFLP technique. Four aluminum tolerant maize lines (15, 16, 699 and 1327) and three non-tolerant ones (19, 53 and 57) were crossed: 57 x 1327, 57 x 699, 53 x 16, 53 x 15 and 19 x 1327. The degree of tolerance to aluminum of the parents, F1's and F2 were determined through measurements of the Relative Seminal Root Length of seedlings grown in nutrient solution containing 222 µmol of Al/L. The RFLP analysis was made in the 6 more tolerant F2 plants and in the 6 more susceptibles plants of each cross, testing two restriction enzymes and five probes from different regions of chromosome 2 of maize, which have loci to Al tolerance. Tolerant and susceptible RFLP different markers were identified to each cross. These markers are potentially useful in marker assisted selection with RFLP's in the genetic breeding for Al tolerance in maize.
publishDate 1996
dc.date.none.fl_str_mv 1996-08-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/4523
url https://seer.sct.embrapa.br/index.php/pab/article/view/4523
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/4523/1809
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.31, n.8, ago. 1996; 575-579
Pesquisa Agropecuária Brasileira; v.31, n.8, ago. 1996; 575-579
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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