Bioprospection of Trichoderma spp. isolates to control Rhizoctonia solani on cucumber seedling production
Autor(a) principal: | |
---|---|
Data de Publicação: | 2010 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Pesquisa Agropecuária Brasileira (Online) |
Texto Completo: | https://seer.sct.embrapa.br/index.php/pab/article/view/1194 |
Resumo: | The objective of this work was to select and identify Trichoderma spp. isolates for the control of Rhizoctonia solani (AG-4) damping-off on cucumber (Cucumis sativus L.) seedlings, as well as to evaluate the effects of increasing concentrations and different combinations of the most effi cient isolates in the disease control. The experiments were carried out in a greenhouse with 490 isolates. The disease on cucumber seedlings was evaluated one week after the application of a commercial substrate infested with both antagonist (1%) and pathogen (1%) to the seedlings’ root collar. The twelve isolates that conferred more than 85% of disease reduction were further evaluated in pathogen control (1%) at the concentrations 0.5, 1, 2, 3 and 4%. The effect of combining fi ve of the most promising isolates in disease control was also evaluated. The most effective isolates were identifi ed through the sequencing of the ribosomal internal transcribed spacers (ITS) region. Out of the 490 isolates tested 44 (9%) caused reduction of damping-off. Antagonist concentrations higher than 2% conferred the most effective disease control. Only two combinations of isolates resulted in increased disease control. The most effective isolates were identifi ed as T. hamatum (IB08, IB30, IB60), T. harzianum (IB34, IB35), T. atroviride (IB13), T. spirale (IB16, IB24) and T. asperellum (IB44). Three isolates could not be identifi ed at species level. |
id |
EMBRAPA-4_873fe959e2507b72512497b99007d88f |
---|---|
oai_identifier_str |
oai:ojs.seer.sct.embrapa.br:article/1194 |
network_acronym_str |
EMBRAPA-4 |
network_name_str |
Pesquisa Agropecuária Brasileira (Online) |
repository_id_str |
|
spelling |
Bioprospection of Trichoderma spp. isolates to control Rhizoctonia solani on cucumber seedling productionBioprospecção de isolados de Trichoderma spp. para o controle de Rhizoctonia solani na produção de mudas de pepinoCucumis sativus; antagonists; biological control; selection method; damping-offCucumis sativus; antagonistas; controle biológico; método de seleção; tombamentoThe objective of this work was to select and identify Trichoderma spp. isolates for the control of Rhizoctonia solani (AG-4) damping-off on cucumber (Cucumis sativus L.) seedlings, as well as to evaluate the effects of increasing concentrations and different combinations of the most effi cient isolates in the disease control. The experiments were carried out in a greenhouse with 490 isolates. The disease on cucumber seedlings was evaluated one week after the application of a commercial substrate infested with both antagonist (1%) and pathogen (1%) to the seedlings’ root collar. The twelve isolates that conferred more than 85% of disease reduction were further evaluated in pathogen control (1%) at the concentrations 0.5, 1, 2, 3 and 4%. The effect of combining fi ve of the most promising isolates in disease control was also evaluated. The most effective isolates were identifi ed through the sequencing of the ribosomal internal transcribed spacers (ITS) region. Out of the 490 isolates tested 44 (9%) caused reduction of damping-off. Antagonist concentrations higher than 2% conferred the most effective disease control. Only two combinations of isolates resulted in increased disease control. The most effective isolates were identifi ed as T. hamatum (IB08, IB30, IB60), T. harzianum (IB34, IB35), T. atroviride (IB13), T. spirale (IB16, IB24) and T. asperellum (IB44). Three isolates could not be identifi ed at species level.O objetivo deste trabalho foi selecionar e identifi car isolados de Trichoderma spp. para o controle do tombamento causado por Rhizoctonia solani (AG-4) em plântulas de pepino (Cucumis sativus L.), além de avaliar o efeito de concentrações crescentes e de combinações dos isolados mais efi cientes no controle da doença. Os experimentos foram conduzidos em casa de vegetação, com 490 isolados. O tombamento das mudas foi avaliado uma semana após a aplicação à base das plântulas de substrato infestado com antagonista (1%) e patógeno (1%). Os doze isolados que proporcionaram mais de 85% de redução da doença foram testados em concentrações crescentes para o controle do patógeno (1%): 0,5, 1, 2, 3 e 4%. Também foi avaliado o efeito das combinações dos cinco isolados mais promissores. Os isolados mais efetivos foram identifi cados pelo sequenciamento da região espaçadores internos transcritos (ITS) do DNA ribossômico. Dos 490 isolados testados 44 (9%) reduziram o tombamento. As concentrações de antagonistas superiores a 2% foram as mais efetivas no controle da doença. Apenas duas combinações resultaram no aumento do controle da doença. Os isolados mais efetivos foram identifi cados como T. hamatum (IB08, IB30, IB60), T. harzianum (IB34, IB35), T. atroviride (IB13), T. spirale (IB16, IB24) e T. asperellum (IB44). Não foi possível a identifi cação da espécie de três isolados.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraFAPESPLucon, Cleusa Maria MantovanelloKoike, Claudia MitsueIshikawa, Alice IshidaPatrício, Flávia Rodrigues AlvesHarakava, Ricardo2010-11-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/1194Pesquisa Agropecuaria Brasileira; v.44, n.3, mar. 2009; 225-232Pesquisa Agropecuária Brasileira; v.44, n.3, mar. 2009; 225-2321678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/1194/5670info:eu-repo/semantics/openAccess2014-12-08T17:48:31Zoai:ojs.seer.sct.embrapa.br:article/1194Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-12-08T17:48:31Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Bioprospection of Trichoderma spp. isolates to control Rhizoctonia solani on cucumber seedling production Bioprospecção de isolados de Trichoderma spp. para o controle de Rhizoctonia solani na produção de mudas de pepino |
title |
Bioprospection of Trichoderma spp. isolates to control Rhizoctonia solani on cucumber seedling production |
spellingShingle |
Bioprospection of Trichoderma spp. isolates to control Rhizoctonia solani on cucumber seedling production Lucon, Cleusa Maria Mantovanello Cucumis sativus; antagonists; biological control; selection method; damping-off Cucumis sativus; antagonistas; controle biológico; método de seleção; tombamento |
title_short |
Bioprospection of Trichoderma spp. isolates to control Rhizoctonia solani on cucumber seedling production |
title_full |
Bioprospection of Trichoderma spp. isolates to control Rhizoctonia solani on cucumber seedling production |
title_fullStr |
Bioprospection of Trichoderma spp. isolates to control Rhizoctonia solani on cucumber seedling production |
title_full_unstemmed |
Bioprospection of Trichoderma spp. isolates to control Rhizoctonia solani on cucumber seedling production |
title_sort |
Bioprospection of Trichoderma spp. isolates to control Rhizoctonia solani on cucumber seedling production |
author |
Lucon, Cleusa Maria Mantovanello |
author_facet |
Lucon, Cleusa Maria Mantovanello Koike, Claudia Mitsue Ishikawa, Alice Ishida Patrício, Flávia Rodrigues Alves Harakava, Ricardo |
author_role |
author |
author2 |
Koike, Claudia Mitsue Ishikawa, Alice Ishida Patrício, Flávia Rodrigues Alves Harakava, Ricardo |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
FAPESP |
dc.contributor.author.fl_str_mv |
Lucon, Cleusa Maria Mantovanello Koike, Claudia Mitsue Ishikawa, Alice Ishida Patrício, Flávia Rodrigues Alves Harakava, Ricardo |
dc.subject.por.fl_str_mv |
Cucumis sativus; antagonists; biological control; selection method; damping-off Cucumis sativus; antagonistas; controle biológico; método de seleção; tombamento |
topic |
Cucumis sativus; antagonists; biological control; selection method; damping-off Cucumis sativus; antagonistas; controle biológico; método de seleção; tombamento |
description |
The objective of this work was to select and identify Trichoderma spp. isolates for the control of Rhizoctonia solani (AG-4) damping-off on cucumber (Cucumis sativus L.) seedlings, as well as to evaluate the effects of increasing concentrations and different combinations of the most effi cient isolates in the disease control. The experiments were carried out in a greenhouse with 490 isolates. The disease on cucumber seedlings was evaluated one week after the application of a commercial substrate infested with both antagonist (1%) and pathogen (1%) to the seedlings’ root collar. The twelve isolates that conferred more than 85% of disease reduction were further evaluated in pathogen control (1%) at the concentrations 0.5, 1, 2, 3 and 4%. The effect of combining fi ve of the most promising isolates in disease control was also evaluated. The most effective isolates were identifi ed through the sequencing of the ribosomal internal transcribed spacers (ITS) region. Out of the 490 isolates tested 44 (9%) caused reduction of damping-off. Antagonist concentrations higher than 2% conferred the most effective disease control. Only two combinations of isolates resulted in increased disease control. The most effective isolates were identifi ed as T. hamatum (IB08, IB30, IB60), T. harzianum (IB34, IB35), T. atroviride (IB13), T. spirale (IB16, IB24) and T. asperellum (IB44). Three isolates could not be identifi ed at species level. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-11-09 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/1194 |
url |
https://seer.sct.embrapa.br/index.php/pab/article/view/1194 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/1194/5670 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
dc.source.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira; v.44, n.3, mar. 2009; 225-232 Pesquisa Agropecuária Brasileira; v.44, n.3, mar. 2009; 225-232 1678-3921 0100-104x reponame:Pesquisa Agropecuária Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Agropecuária Brasileira (Online) |
collection |
Pesquisa Agropecuária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
pab@sct.embrapa.br || sct.pab@embrapa.br |
_version_ |
1793416695256186880 |