Bioprospection of Trichoderma spp. isolates to control Rhizoctonia solani on cucumber seedling production

Detalhes bibliográficos
Autor(a) principal: Lucon, Cleusa Maria Mantovanello
Data de Publicação: 2010
Outros Autores: Koike, Claudia Mitsue, Ishikawa, Alice Ishida, Patrício, Flávia Rodrigues Alves, Harakava, Ricardo
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/1194
Resumo: The objective of this work was to select and identify Trichoderma spp. isolates for the control of Rhizoctonia solani (AG-4) damping-off on cucumber (Cucumis sativus L.) seedlings, as well as to evaluate the effects of increasing concentrations and different combinations of the most effi cient isolates in the disease control. The experiments were carried out in a greenhouse with 490 isolates. The disease on cucumber seedlings was evaluated one week after the application of a commercial substrate infested with both antagonist (1%) and pathogen (1%) to the seedlings’ root collar. The twelve isolates that conferred more than 85% of disease reduction were further evaluated in pathogen control (1%) at the concentrations 0.5, 1, 2, 3 and 4%. The effect of combining fi ve of the most promising isolates in disease control was also evaluated. The most effective isolates were identifi ed through the sequencing of the ribosomal internal transcribed spacers (ITS) region. Out of the 490 isolates tested 44 (9%) caused reduction of damping-off. Antagonist concentrations higher than 2% conferred the most effective disease control. Only two combinations of isolates resulted in increased disease control. The most effective isolates were identifi ed as T. hamatum (IB08, IB30, IB60), T. harzianum (IB34, IB35), T. atroviride (IB13), T. spirale (IB16, IB24) and T. asperellum (IB44). Three isolates could not be identifi ed at species level.
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spelling Bioprospection of Trichoderma spp. isolates to control Rhizoctonia solani on cucumber seedling productionBioprospecção de isolados de Trichoderma spp. para o controle de Rhizoctonia solani na produção de mudas de pepinoCucumis sativus; antagonists; biological control; selection method; damping-offCucumis sativus; antagonistas; controle biológico; método de seleção; tombamentoThe objective of this work was to select and identify Trichoderma spp. isolates for the control of Rhizoctonia solani (AG-4) damping-off on cucumber (Cucumis sativus L.) seedlings, as well as to evaluate the effects of increasing concentrations and different combinations of the most effi cient isolates in the disease control. The experiments were carried out in a greenhouse with 490 isolates. The disease on cucumber seedlings was evaluated one week after the application of a commercial substrate infested with both antagonist (1%) and pathogen (1%) to the seedlings’ root collar. The twelve isolates that conferred more than 85% of disease reduction were further evaluated in pathogen control (1%) at the concentrations 0.5, 1, 2, 3 and 4%. The effect of combining fi ve of the most promising isolates in disease control was also evaluated. The most effective isolates were identifi ed through the sequencing of the ribosomal internal transcribed spacers (ITS) region. Out of the 490 isolates tested 44 (9%) caused reduction of damping-off. Antagonist concentrations higher than 2% conferred the most effective disease control. Only two combinations of isolates resulted in increased disease control. The most effective isolates were identifi ed as T. hamatum (IB08, IB30, IB60), T. harzianum (IB34, IB35), T. atroviride (IB13), T. spirale (IB16, IB24) and T. asperellum (IB44). Three isolates could not be identifi ed at species level.O objetivo deste trabalho foi selecionar e identifi car isolados de Trichoderma spp. para o controle do tombamento causado por Rhizoctonia solani (AG-4) em plântulas de pepino (Cucumis sativus L.), além de avaliar o efeito de concentrações crescentes e de combinações dos isolados mais efi cientes no controle da doença. Os experimentos foram conduzidos em casa de vegetação, com 490 isolados. O tombamento das mudas foi avaliado uma semana após a aplicação à base das plântulas de substrato infestado com antagonista (1%) e patógeno (1%). Os doze isolados que proporcionaram mais de 85% de redução da doença foram testados em concentrações crescentes para o controle do patógeno (1%): 0,5, 1, 2, 3 e 4%. Também foi avaliado o efeito das combinações dos cinco isolados mais promissores. Os isolados mais efetivos foram identifi cados pelo sequenciamento da região espaçadores internos transcritos (ITS) do DNA ribossômico. Dos 490 isolados testados 44 (9%) reduziram o tombamento. As concentrações de antagonistas superiores a 2% foram as mais efetivas no controle da doença. Apenas duas combinações resultaram no aumento do controle da doença. Os isolados mais efetivos foram identifi cados como T. hamatum (IB08, IB30, IB60), T. harzianum (IB34, IB35), T. atroviride (IB13), T. spirale (IB16, IB24) e T. asperellum (IB44). Não foi possível a identifi cação da espécie de três isolados.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraFAPESPLucon, Cleusa Maria MantovanelloKoike, Claudia MitsueIshikawa, Alice IshidaPatrício, Flávia Rodrigues AlvesHarakava, Ricardo2010-11-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/1194Pesquisa Agropecuaria Brasileira; v.44, n.3, mar. 2009; 225-232Pesquisa Agropecuária Brasileira; v.44, n.3, mar. 2009; 225-2321678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/1194/5670info:eu-repo/semantics/openAccess2014-12-08T17:48:31Zoai:ojs.seer.sct.embrapa.br:article/1194Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-12-08T17:48:31Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Bioprospection of Trichoderma spp. isolates to control Rhizoctonia solani on cucumber seedling production
Bioprospecção de isolados de Trichoderma spp. para o controle de Rhizoctonia solani na produção de mudas de pepino
title Bioprospection of Trichoderma spp. isolates to control Rhizoctonia solani on cucumber seedling production
spellingShingle Bioprospection of Trichoderma spp. isolates to control Rhizoctonia solani on cucumber seedling production
Lucon, Cleusa Maria Mantovanello
Cucumis sativus; antagonists; biological control; selection method; damping-off
Cucumis sativus; antagonistas; controle biológico; método de seleção; tombamento
title_short Bioprospection of Trichoderma spp. isolates to control Rhizoctonia solani on cucumber seedling production
title_full Bioprospection of Trichoderma spp. isolates to control Rhizoctonia solani on cucumber seedling production
title_fullStr Bioprospection of Trichoderma spp. isolates to control Rhizoctonia solani on cucumber seedling production
title_full_unstemmed Bioprospection of Trichoderma spp. isolates to control Rhizoctonia solani on cucumber seedling production
title_sort Bioprospection of Trichoderma spp. isolates to control Rhizoctonia solani on cucumber seedling production
author Lucon, Cleusa Maria Mantovanello
author_facet Lucon, Cleusa Maria Mantovanello
Koike, Claudia Mitsue
Ishikawa, Alice Ishida
Patrício, Flávia Rodrigues Alves
Harakava, Ricardo
author_role author
author2 Koike, Claudia Mitsue
Ishikawa, Alice Ishida
Patrício, Flávia Rodrigues Alves
Harakava, Ricardo
author2_role author
author
author
author
dc.contributor.none.fl_str_mv
FAPESP
dc.contributor.author.fl_str_mv Lucon, Cleusa Maria Mantovanello
Koike, Claudia Mitsue
Ishikawa, Alice Ishida
Patrício, Flávia Rodrigues Alves
Harakava, Ricardo
dc.subject.por.fl_str_mv Cucumis sativus; antagonists; biological control; selection method; damping-off
Cucumis sativus; antagonistas; controle biológico; método de seleção; tombamento
topic Cucumis sativus; antagonists; biological control; selection method; damping-off
Cucumis sativus; antagonistas; controle biológico; método de seleção; tombamento
description The objective of this work was to select and identify Trichoderma spp. isolates for the control of Rhizoctonia solani (AG-4) damping-off on cucumber (Cucumis sativus L.) seedlings, as well as to evaluate the effects of increasing concentrations and different combinations of the most effi cient isolates in the disease control. The experiments were carried out in a greenhouse with 490 isolates. The disease on cucumber seedlings was evaluated one week after the application of a commercial substrate infested with both antagonist (1%) and pathogen (1%) to the seedlings’ root collar. The twelve isolates that conferred more than 85% of disease reduction were further evaluated in pathogen control (1%) at the concentrations 0.5, 1, 2, 3 and 4%. The effect of combining fi ve of the most promising isolates in disease control was also evaluated. The most effective isolates were identifi ed through the sequencing of the ribosomal internal transcribed spacers (ITS) region. Out of the 490 isolates tested 44 (9%) caused reduction of damping-off. Antagonist concentrations higher than 2% conferred the most effective disease control. Only two combinations of isolates resulted in increased disease control. The most effective isolates were identifi ed as T. hamatum (IB08, IB30, IB60), T. harzianum (IB34, IB35), T. atroviride (IB13), T. spirale (IB16, IB24) and T. asperellum (IB44). Three isolates could not be identifi ed at species level.
publishDate 2010
dc.date.none.fl_str_mv 2010-11-09
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/1194
url https://seer.sct.embrapa.br/index.php/pab/article/view/1194
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/1194/5670
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.44, n.3, mar. 2009; 225-232
Pesquisa Agropecuária Brasileira; v.44, n.3, mar. 2009; 225-232
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
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instacron:EMBRAPA
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repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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