Isolation of a cotton NADP(H) oxidase homologue induced by drought stress
Autor(a) principal: | |
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Data de Publicação: | 2000 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Agropecuária Brasileira (Online) |
Texto Completo: | https://seer.sct.embrapa.br/index.php/pab/article/view/5914 |
Resumo: | The aim of this study was to identify and isolate genes that are differentially expressed in four selected cotton (Gossypium hirsutum L.) genotypes contrasting according to their tolerance to water deficit. The genotypes studied were Siokra L-23, Stoneville 506, CS 50 and T-1521. Physiological, morphological and developmental changes that confer drought tolerance in plants must have a molecular genetic basis. To identify and isolate the genes, the mRNA Differential Display (DD) technique was used. Messenger RNAs differentially expressed during water deficit were identified, isolated, cloned and sequenced. The cloned transcript A12B15-5, a NADP(H) oxidase homologue, was up regulated only during the water deficit stress and only in Siokra L-23, a drought tolerant genotype. Ribonuclease protection assay confirmed that transcription. |
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Isolation of a cotton NADP(H) oxidase homologue induced by drought stressIsolamento de um cDNA em algodão homólogo a uma NADP(H) oxidase e induzido durante a secamessenger RNA; gene expression; genetic transformation; water deficitRNA mensageiro; expressão gênica; transformação genética; déficit hídricoThe aim of this study was to identify and isolate genes that are differentially expressed in four selected cotton (Gossypium hirsutum L.) genotypes contrasting according to their tolerance to water deficit. The genotypes studied were Siokra L-23, Stoneville 506, CS 50 and T-1521. Physiological, morphological and developmental changes that confer drought tolerance in plants must have a molecular genetic basis. To identify and isolate the genes, the mRNA Differential Display (DD) technique was used. Messenger RNAs differentially expressed during water deficit were identified, isolated, cloned and sequenced. The cloned transcript A12B15-5, a NADP(H) oxidase homologue, was up regulated only during the water deficit stress and only in Siokra L-23, a drought tolerant genotype. Ribonuclease protection assay confirmed that transcription.O objetivo deste estudo foi identificar e isolar genes diferencialmente expressos em quatro genótipos de algodão (Gossypium hirsutum L.) que apresentam respostas distintas ao déficit hídrico. Os genótipos estudados foram Siokra L-23, Stoneville 506, CS 50 e T-1521. As mudanças fisiológicas, morfológicas e de desenvolvimento que as plantas apresentam para suportar a diminuição na disponibilidade de água tem, obrigatoriamente, uma base molecular genética. Utilizou-se a técnica de "Differential Display" para identificar e isolar os genes diferencialmente expressos. RNAs mensageiros diferencialmente expressos durante o déficit hídrico foram identificados, isolados, clonados e seqüenciados. O clone A12B15-5 apresentou homologia com uma NADP(H) oxidase e foi expresso somente durante o período de déficit hídrico em Siokra L-23, um genótipo de algodão tolerante à seca. O uso da técnica "Ribonuclease Protection Assay" confirmou essa expressão.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraNepomuceno, Alexandre LimaStewart, James MCDOosterhuis, DerrickTurley, RickieNeumaier, NormanFaria, José Renato Bouças2000-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/5914Pesquisa Agropecuaria Brasileira; v.35, n.7, jul. 2000; 1407-1416Pesquisa Agropecuária Brasileira; v.35, n.7, jul. 2000; 1407-14161678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/5914/3018info:eu-repo/semantics/openAccess2014-08-19T16:25:28Zoai:ojs.seer.sct.embrapa.br:article/5914Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-08-19T16:25:28Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Isolation of a cotton NADP(H) oxidase homologue induced by drought stress Isolamento de um cDNA em algodão homólogo a uma NADP(H) oxidase e induzido durante a seca |
title |
Isolation of a cotton NADP(H) oxidase homologue induced by drought stress |
spellingShingle |
Isolation of a cotton NADP(H) oxidase homologue induced by drought stress Nepomuceno, Alexandre Lima messenger RNA; gene expression; genetic transformation; water deficit RNA mensageiro; expressão gênica; transformação genética; déficit hídrico |
title_short |
Isolation of a cotton NADP(H) oxidase homologue induced by drought stress |
title_full |
Isolation of a cotton NADP(H) oxidase homologue induced by drought stress |
title_fullStr |
Isolation of a cotton NADP(H) oxidase homologue induced by drought stress |
title_full_unstemmed |
Isolation of a cotton NADP(H) oxidase homologue induced by drought stress |
title_sort |
Isolation of a cotton NADP(H) oxidase homologue induced by drought stress |
author |
Nepomuceno, Alexandre Lima |
author_facet |
Nepomuceno, Alexandre Lima Stewart, James MCD Oosterhuis, Derrick Turley, Rickie Neumaier, Norman Faria, José Renato Bouças |
author_role |
author |
author2 |
Stewart, James MCD Oosterhuis, Derrick Turley, Rickie Neumaier, Norman Faria, José Renato Bouças |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
|
dc.contributor.author.fl_str_mv |
Nepomuceno, Alexandre Lima Stewart, James MCD Oosterhuis, Derrick Turley, Rickie Neumaier, Norman Faria, José Renato Bouças |
dc.subject.por.fl_str_mv |
messenger RNA; gene expression; genetic transformation; water deficit RNA mensageiro; expressão gênica; transformação genética; déficit hídrico |
topic |
messenger RNA; gene expression; genetic transformation; water deficit RNA mensageiro; expressão gênica; transformação genética; déficit hídrico |
description |
The aim of this study was to identify and isolate genes that are differentially expressed in four selected cotton (Gossypium hirsutum L.) genotypes contrasting according to their tolerance to water deficit. The genotypes studied were Siokra L-23, Stoneville 506, CS 50 and T-1521. Physiological, morphological and developmental changes that confer drought tolerance in plants must have a molecular genetic basis. To identify and isolate the genes, the mRNA Differential Display (DD) technique was used. Messenger RNAs differentially expressed during water deficit were identified, isolated, cloned and sequenced. The cloned transcript A12B15-5, a NADP(H) oxidase homologue, was up regulated only during the water deficit stress and only in Siokra L-23, a drought tolerant genotype. Ribonuclease protection assay confirmed that transcription. |
publishDate |
2000 |
dc.date.none.fl_str_mv |
2000-07-01 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/5914 |
url |
https://seer.sct.embrapa.br/index.php/pab/article/view/5914 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/5914/3018 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
dc.source.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira; v.35, n.7, jul. 2000; 1407-1416 Pesquisa Agropecuária Brasileira; v.35, n.7, jul. 2000; 1407-1416 1678-3921 0100-104x reponame:Pesquisa Agropecuária Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Agropecuária Brasileira (Online) |
collection |
Pesquisa Agropecuária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
pab@sct.embrapa.br || sct.pab@embrapa.br |
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1793416650027958272 |