Isolation of a cotton NADP(H) oxidase homologue induced by drought stress

Detalhes bibliográficos
Autor(a) principal: Nepomuceno, Alexandre Lima
Data de Publicação: 2000
Outros Autores: Stewart, James MCD, Oosterhuis, Derrick, Turley, Rickie, Neumaier, Norman, Faria, José Renato Bouças
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/5914
Resumo: The aim of this study was to identify and isolate genes that are differentially expressed in four selected cotton (Gossypium hirsutum L.) genotypes contrasting according to their tolerance to water deficit. The genotypes studied were Siokra L-23, Stoneville 506, CS 50 and T-1521. Physiological, morphological and developmental changes that confer drought tolerance in plants must have a molecular genetic basis. To identify and isolate the genes, the mRNA Differential Display (DD) technique was used. Messenger RNAs differentially expressed during water deficit were identified, isolated, cloned and sequenced. The cloned transcript A12B15-5, a NADP(H) oxidase homologue, was up regulated only during the water deficit stress and only in Siokra L-23, a drought tolerant genotype. Ribonuclease protection assay confirmed that transcription.
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spelling Isolation of a cotton NADP(H) oxidase homologue induced by drought stressIsolamento de um cDNA em algodão homólogo a uma NADP(H) oxidase e induzido durante a secamessenger RNA; gene expression; genetic transformation; water deficitRNA mensageiro; expressão gênica; transformação genética; déficit hídricoThe aim of this study was to identify and isolate genes that are differentially expressed in four selected cotton (Gossypium hirsutum L.) genotypes contrasting according to their tolerance to water deficit. The genotypes studied were Siokra L-23, Stoneville 506, CS 50 and T-1521. Physiological, morphological and developmental changes that confer drought tolerance in plants must have a molecular genetic basis. To identify and isolate the genes, the mRNA Differential Display (DD) technique was used. Messenger RNAs differentially expressed during water deficit were identified, isolated, cloned and sequenced. The cloned transcript A12B15-5, a NADP(H) oxidase homologue, was up regulated only during the water deficit stress and only in Siokra L-23, a drought tolerant genotype. Ribonuclease protection assay confirmed that transcription.O objetivo deste estudo foi identificar e isolar genes diferencialmente expressos em quatro genótipos de algodão (Gossypium hirsutum L.) que apresentam respostas distintas ao déficit hídrico. Os genótipos estudados foram Siokra L-23, Stoneville 506, CS 50 e T-1521. As mudanças fisiológicas, morfológicas e de desenvolvimento que as plantas apresentam para suportar a diminuição na disponibilidade de água tem, obrigatoriamente, uma base molecular genética. Utilizou-se a técnica de "Differential Display" para identificar e isolar os genes diferencialmente expressos. RNAs mensageiros diferencialmente expressos durante o déficit hídrico foram identificados, isolados, clonados e seqüenciados. O clone A12B15-5 apresentou homologia com uma NADP(H) oxidase e foi expresso somente durante o período de déficit hídrico em Siokra L-23, um genótipo de algodão tolerante à seca. O uso da técnica "Ribonuclease Protection Assay" confirmou essa expressão.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraNepomuceno, Alexandre LimaStewart, James MCDOosterhuis, DerrickTurley, RickieNeumaier, NormanFaria, José Renato Bouças2000-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/5914Pesquisa Agropecuaria Brasileira; v.35, n.7, jul. 2000; 1407-1416Pesquisa Agropecuária Brasileira; v.35, n.7, jul. 2000; 1407-14161678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/5914/3018info:eu-repo/semantics/openAccess2014-08-19T16:25:28Zoai:ojs.seer.sct.embrapa.br:article/5914Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-08-19T16:25:28Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Isolation of a cotton NADP(H) oxidase homologue induced by drought stress
Isolamento de um cDNA em algodão homólogo a uma NADP(H) oxidase e induzido durante a seca
title Isolation of a cotton NADP(H) oxidase homologue induced by drought stress
spellingShingle Isolation of a cotton NADP(H) oxidase homologue induced by drought stress
Nepomuceno, Alexandre Lima
messenger RNA; gene expression; genetic transformation; water deficit
RNA mensageiro; expressão gênica; transformação genética; déficit hídrico
title_short Isolation of a cotton NADP(H) oxidase homologue induced by drought stress
title_full Isolation of a cotton NADP(H) oxidase homologue induced by drought stress
title_fullStr Isolation of a cotton NADP(H) oxidase homologue induced by drought stress
title_full_unstemmed Isolation of a cotton NADP(H) oxidase homologue induced by drought stress
title_sort Isolation of a cotton NADP(H) oxidase homologue induced by drought stress
author Nepomuceno, Alexandre Lima
author_facet Nepomuceno, Alexandre Lima
Stewart, James MCD
Oosterhuis, Derrick
Turley, Rickie
Neumaier, Norman
Faria, José Renato Bouças
author_role author
author2 Stewart, James MCD
Oosterhuis, Derrick
Turley, Rickie
Neumaier, Norman
Faria, José Renato Bouças
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Nepomuceno, Alexandre Lima
Stewart, James MCD
Oosterhuis, Derrick
Turley, Rickie
Neumaier, Norman
Faria, José Renato Bouças
dc.subject.por.fl_str_mv messenger RNA; gene expression; genetic transformation; water deficit
RNA mensageiro; expressão gênica; transformação genética; déficit hídrico
topic messenger RNA; gene expression; genetic transformation; water deficit
RNA mensageiro; expressão gênica; transformação genética; déficit hídrico
description The aim of this study was to identify and isolate genes that are differentially expressed in four selected cotton (Gossypium hirsutum L.) genotypes contrasting according to their tolerance to water deficit. The genotypes studied were Siokra L-23, Stoneville 506, CS 50 and T-1521. Physiological, morphological and developmental changes that confer drought tolerance in plants must have a molecular genetic basis. To identify and isolate the genes, the mRNA Differential Display (DD) technique was used. Messenger RNAs differentially expressed during water deficit were identified, isolated, cloned and sequenced. The cloned transcript A12B15-5, a NADP(H) oxidase homologue, was up regulated only during the water deficit stress and only in Siokra L-23, a drought tolerant genotype. Ribonuclease protection assay confirmed that transcription.
publishDate 2000
dc.date.none.fl_str_mv 2000-07-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/5914
url https://seer.sct.embrapa.br/index.php/pab/article/view/5914
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/5914/3018
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.35, n.7, jul. 2000; 1407-1416
Pesquisa Agropecuária Brasileira; v.35, n.7, jul. 2000; 1407-1416
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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