Trypanosoma cruzi XRNA granules colocalise with distinct mRNP granules at the nuclear periphery

Detalhes bibliográficos
Autor(a) principal: Costa, Jimena Ferreira da
Data de Publicação: 2018
Outros Autores: Ferrarini, Mariana Galvão, Nardelli, Sheila Cristina, Goldenberg, Samuel, Ávila, Andréa Rodrigues, Holetz, Fabíola Barbieri
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/28924
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
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spelling Costa, Jimena Ferreira daFerrarini, Mariana GalvãoNardelli, Sheila CristinaGoldenberg, SamuelÁvila, Andréa RodriguesHoletz, Fabíola Barbieri2018-09-20T12:10:31Z2018-09-20T12:10:31Z2018COSTA, Jimena Ferreira da et al.Trypanosoma cruzi XRNA granules colocalise with distinct mRNP granules at the nuclear periphery. Mem. Inst. Oswaldo Cruz, Rio de Janeiro, v.113, n. 6, 2018.0074-0276https://www.arca.fiocruz.br/handle/icict/2892410.1590/0074-027601705311678-8060porFundação Oswaldo Cruz. Instituto Oswaldo CruzTrypanosoma cruziRNAMessengerRibonucleoproteinsGene ExpressionARN MensajeroExpresión GénicaRNA MensageiroRibonucleoproteínasExpressão GênicaTrypanosoma cruzi XRNA granules colocalise with distinct mRNP granules at the nuclear peripheryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Eukaryotic ribonucleoprotein (RNP) granules are important for the regulation of RNA fate. RNP granules exist in trypanosomatids; however, their roles in controlling gene expression are still not understood. XRNA is a component of granules in Trypanosoma brucei but has not been investigated in Trypanosoma cruzi. This study aimed to investigate the TcXRNA dynamic assembly and its interaction with RNP components under conditions that affect the mRNA availability. As methods, we used in vitro metacyclogenesis of T. cruzi to observe changes in RNP granules during the differentiation process. TcXRNA expression was analysed by Western blot and immunofluorescence. Colocalisation assays were performed to investigate the interaction of TcXRNA with other RNP components. It finded that TcXRNA is constantly present during metacyclogenesis and is localised in cytoplasmic granules. TcXRNA does not colocalise with TcDHH1 and TcCAF1 granules in the cytoplasm. However, TcXRNA granules colocalise with mRNP granules at the nuclear periphery when mRNA processing is inhibited. This way we have as main conclusions that, TcXRNA plays a role in mRNA metabolism as a component of mRNP granules whose assembly is dependent on mRNA availability. TcXRNA granules colocalise with distinct RNP granules at the nuclear periphery, suggesting that the perinuclear region is a regulatory compartment in T. cruzi mRNA metabolism.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/28924/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALMemoriasIOC-170531.pdfMemoriasIOC-170531.pdfapplication/pdf2249280https://www.arca.fiocruz.br/bitstream/icict/28924/2/MemoriasIOC-170531.pdfd8ac6e8c141bef5b3b17e0564ee4cf4bMD52TEXTMemoriasIOC-170531.pdf.txtMemoriasIOC-170531.pdf.txtExtracted texttext/plain38731https://www.arca.fiocruz.br/bitstream/icict/28924/3/MemoriasIOC-170531.pdf.txt4aa3dd429e302ed080811c1bb6e0038eMD53icict/289242018-09-21 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dc.title.pt_BR.fl_str_mv Trypanosoma cruzi XRNA granules colocalise with distinct mRNP granules at the nuclear periphery
title Trypanosoma cruzi XRNA granules colocalise with distinct mRNP granules at the nuclear periphery
spellingShingle Trypanosoma cruzi XRNA granules colocalise with distinct mRNP granules at the nuclear periphery
Costa, Jimena Ferreira da
Trypanosoma cruzi
RNA
Messenger
Ribonucleoproteins
Gene Expression
ARN Mensajero
Expresión Génica
RNA Mensageiro
Ribonucleoproteínas
Expressão Gênica
title_short Trypanosoma cruzi XRNA granules colocalise with distinct mRNP granules at the nuclear periphery
title_full Trypanosoma cruzi XRNA granules colocalise with distinct mRNP granules at the nuclear periphery
title_fullStr Trypanosoma cruzi XRNA granules colocalise with distinct mRNP granules at the nuclear periphery
title_full_unstemmed Trypanosoma cruzi XRNA granules colocalise with distinct mRNP granules at the nuclear periphery
title_sort Trypanosoma cruzi XRNA granules colocalise with distinct mRNP granules at the nuclear periphery
author Costa, Jimena Ferreira da
author_facet Costa, Jimena Ferreira da
Ferrarini, Mariana Galvão
Nardelli, Sheila Cristina
Goldenberg, Samuel
Ávila, Andréa Rodrigues
Holetz, Fabíola Barbieri
author_role author
author2 Ferrarini, Mariana Galvão
Nardelli, Sheila Cristina
Goldenberg, Samuel
Ávila, Andréa Rodrigues
Holetz, Fabíola Barbieri
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Costa, Jimena Ferreira da
Ferrarini, Mariana Galvão
Nardelli, Sheila Cristina
Goldenberg, Samuel
Ávila, Andréa Rodrigues
Holetz, Fabíola Barbieri
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
topic Trypanosoma cruzi
RNA
Messenger
Ribonucleoproteins
Gene Expression
ARN Mensajero
Expresión Génica
RNA Mensageiro
Ribonucleoproteínas
Expressão Gênica
dc.subject.en.pt_BR.fl_str_mv RNA
Messenger
Ribonucleoproteins
Gene Expression
dc.subject.es.pt_BR.fl_str_mv ARN Mensajero
Expresión Génica
dc.subject.decs.pt_BR.fl_str_mv RNA Mensageiro
Ribonucleoproteínas
Expressão Gênica
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-09-20T12:10:31Z
dc.date.available.fl_str_mv 2018-09-20T12:10:31Z
dc.date.issued.fl_str_mv 2018
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv COSTA, Jimena Ferreira da et al.Trypanosoma cruzi XRNA granules colocalise with distinct mRNP granules at the nuclear periphery. Mem. Inst. Oswaldo Cruz, Rio de Janeiro, v.113, n. 6, 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/28924
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/0074-02760170531
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv COSTA, Jimena Ferreira da et al.Trypanosoma cruzi XRNA granules colocalise with distinct mRNP granules at the nuclear periphery. Mem. Inst. Oswaldo Cruz, Rio de Janeiro, v.113, n. 6, 2018.
0074-0276
10.1590/0074-02760170531
1678-8060
url https://www.arca.fiocruz.br/handle/icict/28924
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/28924/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/28924/2/MemoriasIOC-170531.pdf
https://www.arca.fiocruz.br/bitstream/icict/28924/3/MemoriasIOC-170531.pdf.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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