Genetic diversity among hybrids of sweet orange and 'Murcott' tangor evaluated by fAFLP and RAPD markers

Detalhes bibliográficos
Autor(a) principal: Bastianel, Marinês
Data de Publicação: 2006
Outros Autores: Oliveira, Antonio Carlos de, Cristofani, Mariângela, Machado, Marcos Antônio
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/7206
Resumo: The objective of this work was to evaluate the genetic diversity in a population of 148 hybrids of 'Murcott' tangor (Citrus reticulata Blanco x C. sinensis L. Osbeck) and 'Pêra' sweet orange (C. sinensis L. Osbeck), obtained by controlled polination, using fAFLP and RAPD markers. Polymorphic markers (416 fAFLP and 33 RAPD markers) were used to evaluate genetic similarity among the hybrids, calculated by the coefficient of Jaccard, using the UPGMA method. The consistency of each group was determined by software BOOD. There was high genetic similarity within the parents. 'Pêra' sweet orange had a higher number of loci in heterozygosis (132) compared to 'Murcott' tangor (105), supporting the theory of hybrid origin for sweet oranges. Two distinct groups of plants were observed: one group had 80% of the hybrids that displayed higher genetic similarity with 'Pêra' sweet orange. The bootstrap analysis did not show consistence between the groups. The fAFLP markers are more efficient to obtain polymorphism than RAPDs and are more suitable to select hybrids closer to the parents.
id EMBRAPA-4_9782a5392e9cfbea390cab6a299a76cb
oai_identifier_str oai:ojs.seer.sct.embrapa.br:article/7206
network_acronym_str EMBRAPA-4
network_name_str Pesquisa Agropecuária Brasileira (Online)
repository_id_str
spelling Genetic diversity among hybrids of sweet orange and 'Murcott' tangor evaluated by fAFLP and RAPD markersDiversidade genética entre híbridos de laranja-doce e tangor 'Murcott' avaliada por fAFLP e RAPDcitrus; molecular markers; genetic similaritycitros; marcadores moleculares; similaridade genéticaThe objective of this work was to evaluate the genetic diversity in a population of 148 hybrids of 'Murcott' tangor (Citrus reticulata Blanco x C. sinensis L. Osbeck) and 'Pêra' sweet orange (C. sinensis L. Osbeck), obtained by controlled polination, using fAFLP and RAPD markers. Polymorphic markers (416 fAFLP and 33 RAPD markers) were used to evaluate genetic similarity among the hybrids, calculated by the coefficient of Jaccard, using the UPGMA method. The consistency of each group was determined by software BOOD. There was high genetic similarity within the parents. 'Pêra' sweet orange had a higher number of loci in heterozygosis (132) compared to 'Murcott' tangor (105), supporting the theory of hybrid origin for sweet oranges. Two distinct groups of plants were observed: one group had 80% of the hybrids that displayed higher genetic similarity with 'Pêra' sweet orange. The bootstrap analysis did not show consistence between the groups. The fAFLP markers are more efficient to obtain polymorphism than RAPDs and are more suitable to select hybrids closer to the parents.O objetivo deste trabalho foi avaliar a diversidade genética em uma população de 148 híbridos de tangor 'Murcott' (Citrus reticulata Blanco x C. sinensis L. Osbeck) e laranja 'Pêra' (C. sinensis L. Osbeck) obtidos por polinização controlada, pelo uso de marcadores fAFLP e RAPD. Marcadores polimórficos (416 marcadores fAFLP e 33 RAPD) foram utilizados para avaliar a similaridade genética entre os híbridos, calculada com o coeficiente Jaccard pelo método UPGMA. A consistência de cada agrupamento foi determinada pelo programa BOOD. Houve alta similaridade genética entre os parentais. A laranja 'Pêra' apresentou maior número (132) de loci em heterozigose em relação ao tangor 'Murcott' (105), corroborando a teoria de origem híbrida para a laranja-doce. Observaram-se dois grupos distintos de plantas, e um deles abrangeu 80% dos híbridos com maior similaridade com a laranja 'Pêra'. A análise bootstrap não revelou consistência estatística entre esses grupos. Marcadores fAFLP são mais eficientes na avaliação do polimorfismo, sendo indicados para seleção de indivíduos híbridos mais próximos a um dos parentais.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraBastianel, MarinêsOliveira, Antonio Carlos deCristofani, MariângelaMachado, Marcos Antônio2006-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/7206Pesquisa Agropecuaria Brasileira; v.41, n.5, maio 2006; 779-784Pesquisa Agropecuária Brasileira; v.41, n.5, maio 2006; 779-7841678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/7206/4251info:eu-repo/semantics/openAccess2010-12-02T14:10:49Zoai:ojs.seer.sct.embrapa.br:article/7206Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2010-12-02T14:10:49Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Genetic diversity among hybrids of sweet orange and 'Murcott' tangor evaluated by fAFLP and RAPD markers
Diversidade genética entre híbridos de laranja-doce e tangor 'Murcott' avaliada por fAFLP e RAPD
title Genetic diversity among hybrids of sweet orange and 'Murcott' tangor evaluated by fAFLP and RAPD markers
spellingShingle Genetic diversity among hybrids of sweet orange and 'Murcott' tangor evaluated by fAFLP and RAPD markers
Bastianel, Marinês
citrus; molecular markers; genetic similarity
citros; marcadores moleculares; similaridade genética
title_short Genetic diversity among hybrids of sweet orange and 'Murcott' tangor evaluated by fAFLP and RAPD markers
title_full Genetic diversity among hybrids of sweet orange and 'Murcott' tangor evaluated by fAFLP and RAPD markers
title_fullStr Genetic diversity among hybrids of sweet orange and 'Murcott' tangor evaluated by fAFLP and RAPD markers
title_full_unstemmed Genetic diversity among hybrids of sweet orange and 'Murcott' tangor evaluated by fAFLP and RAPD markers
title_sort Genetic diversity among hybrids of sweet orange and 'Murcott' tangor evaluated by fAFLP and RAPD markers
author Bastianel, Marinês
author_facet Bastianel, Marinês
Oliveira, Antonio Carlos de
Cristofani, Mariângela
Machado, Marcos Antônio
author_role author
author2 Oliveira, Antonio Carlos de
Cristofani, Mariângela
Machado, Marcos Antônio
author2_role author
author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Bastianel, Marinês
Oliveira, Antonio Carlos de
Cristofani, Mariângela
Machado, Marcos Antônio
dc.subject.por.fl_str_mv citrus; molecular markers; genetic similarity
citros; marcadores moleculares; similaridade genética
topic citrus; molecular markers; genetic similarity
citros; marcadores moleculares; similaridade genética
description The objective of this work was to evaluate the genetic diversity in a population of 148 hybrids of 'Murcott' tangor (Citrus reticulata Blanco x C. sinensis L. Osbeck) and 'Pêra' sweet orange (C. sinensis L. Osbeck), obtained by controlled polination, using fAFLP and RAPD markers. Polymorphic markers (416 fAFLP and 33 RAPD markers) were used to evaluate genetic similarity among the hybrids, calculated by the coefficient of Jaccard, using the UPGMA method. The consistency of each group was determined by software BOOD. There was high genetic similarity within the parents. 'Pêra' sweet orange had a higher number of loci in heterozygosis (132) compared to 'Murcott' tangor (105), supporting the theory of hybrid origin for sweet oranges. Two distinct groups of plants were observed: one group had 80% of the hybrids that displayed higher genetic similarity with 'Pêra' sweet orange. The bootstrap analysis did not show consistence between the groups. The fAFLP markers are more efficient to obtain polymorphism than RAPDs and are more suitable to select hybrids closer to the parents.
publishDate 2006
dc.date.none.fl_str_mv 2006-05-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/7206
url https://seer.sct.embrapa.br/index.php/pab/article/view/7206
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/7206/4251
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.41, n.5, maio 2006; 779-784
Pesquisa Agropecuária Brasileira; v.41, n.5, maio 2006; 779-784
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
_version_ 1793416652977602560

Registros relacionados