Genetic linkage maps of 'Pêra' sweet orange and 'Cravo' mandarin with RAPD markers
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Agropecuária Brasileira (Online) |
Texto Completo: | https://seer.sct.embrapa.br/index.php/pab/article/view/6751 |
Resumo: | The objective of this work was to construct linkage maps of ‘Pêra’ sweet orange [Citrus sinensis (L.) Osbeck] and ‘Cravo’ mandarin (Citrus reticulata Blanco) using RAPD markers and the pseudo-testcross strategy. The parents were chosen according to the resistance/susceptibility to citrus variegate chlorosis (CVC). The segregation of 176 markers was analyzed in 94 progeny of F1 hybrids, which were obtained from controlled crossings. The linkage map of ‘Pêra’ sweet orange had 117 markers defined by 12 linkage groups, which spanned 612.1 cM. Only six markers could not be linked to the linkage group and 48.7% of the markers showed segregation distortion. The linkage map of ‘Cravo’ mandarin had 51 markers defined by 12 linkage groups, which spanned 353.3 cM. Only two markers did not link to the groups and 15.7% showed segregation distortion. The construction of linkage maps is relevant to future mapping studies of the inheritance of CVC, citrus canker and leprosis. |
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Genetic linkage maps of 'Pêra' sweet orange and 'Cravo' mandarin with RAPD markersMapas genéticos de ligação de laranja 'Pêra' e tangerina 'Cravo' utilizando marcadores RAPDcitrus; dominant markers; molecular markers; segregation; breeding methodscitro; marcador dominante; marcador molecular; segregação; método de melhoramentoThe objective of this work was to construct linkage maps of ‘Pêra’ sweet orange [Citrus sinensis (L.) Osbeck] and ‘Cravo’ mandarin (Citrus reticulata Blanco) using RAPD markers and the pseudo-testcross strategy. The parents were chosen according to the resistance/susceptibility to citrus variegate chlorosis (CVC). The segregation of 176 markers was analyzed in 94 progeny of F1 hybrids, which were obtained from controlled crossings. The linkage map of ‘Pêra’ sweet orange had 117 markers defined by 12 linkage groups, which spanned 612.1 cM. Only six markers could not be linked to the linkage group and 48.7% of the markers showed segregation distortion. The linkage map of ‘Cravo’ mandarin had 51 markers defined by 12 linkage groups, which spanned 353.3 cM. Only two markers did not link to the groups and 15.7% showed segregation distortion. The construction of linkage maps is relevant to future mapping studies of the inheritance of CVC, citrus canker and leprosis.O objetivo deste trabalho foi elaborar mapas de ligação de laranja ‘Pêra’ [Citrus sinensis (L.) Osbeck] e tangerina ‘Cravo’ (Citrus reticulata Blanco) utilizando marcadores RAPD e a estratégia pseudo-testcross. Os parentais foram escolhidos tomando-se por base o nível de resistência/suscetibilidade à clorose variegada dos citros (CVC). A segregação de 176 marcadores foi analisada em uma progênie de 94 híbridos F1, obtidos por meio de cruzamentos controlados. O mapa de ligação de laranja ‘Pêra’ apresentou 117 marcadores distribuídos em 12 grupos de ligação, totalizando 612,1 cM. Apenas seis marcadores não se ligaram a algum dos grupos de ligação e 48,7% deles apresentaram segregação distorcida. O mapa de ligação de tangerina ‘Cravo’ apresentou 51 marcadores distribuídos em 12 grupos de ligação, totalizando 353,3 cM. Apenas dois marcadores apresentaram-se desligados e 15,7% deles apresentaram segregação distorcida. A elaboração de mapas de ligação dessas cultivares é relevante para futuros estudos da herança da resistência à CVC, cancro cítrico e leprose.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária Brasileirade Oliveira, Roberto PedrosoCristofani, MariângelaMachado, Marcos Antônio2004-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/6751Pesquisa Agropecuaria Brasileira; v.39, n.2, fev. 2004; 159-165Pesquisa Agropecuária Brasileira; v.39, n.2, fev. 2004; 159-1651678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/6751/3807info:eu-repo/semantics/openAccess2014-07-03T18:23:54Zoai:ojs.seer.sct.embrapa.br:article/6751Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-07-03T18:23:54Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Genetic linkage maps of 'Pêra' sweet orange and 'Cravo' mandarin with RAPD markers Mapas genéticos de ligação de laranja 'Pêra' e tangerina 'Cravo' utilizando marcadores RAPD |
title |
Genetic linkage maps of 'Pêra' sweet orange and 'Cravo' mandarin with RAPD markers |
spellingShingle |
Genetic linkage maps of 'Pêra' sweet orange and 'Cravo' mandarin with RAPD markers de Oliveira, Roberto Pedroso citrus; dominant markers; molecular markers; segregation; breeding methods citro; marcador dominante; marcador molecular; segregação; método de melhoramento |
title_short |
Genetic linkage maps of 'Pêra' sweet orange and 'Cravo' mandarin with RAPD markers |
title_full |
Genetic linkage maps of 'Pêra' sweet orange and 'Cravo' mandarin with RAPD markers |
title_fullStr |
Genetic linkage maps of 'Pêra' sweet orange and 'Cravo' mandarin with RAPD markers |
title_full_unstemmed |
Genetic linkage maps of 'Pêra' sweet orange and 'Cravo' mandarin with RAPD markers |
title_sort |
Genetic linkage maps of 'Pêra' sweet orange and 'Cravo' mandarin with RAPD markers |
author |
de Oliveira, Roberto Pedroso |
author_facet |
de Oliveira, Roberto Pedroso Cristofani, Mariângela Machado, Marcos Antônio |
author_role |
author |
author2 |
Cristofani, Mariângela Machado, Marcos Antônio |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
|
dc.contributor.author.fl_str_mv |
de Oliveira, Roberto Pedroso Cristofani, Mariângela Machado, Marcos Antônio |
dc.subject.por.fl_str_mv |
citrus; dominant markers; molecular markers; segregation; breeding methods citro; marcador dominante; marcador molecular; segregação; método de melhoramento |
topic |
citrus; dominant markers; molecular markers; segregation; breeding methods citro; marcador dominante; marcador molecular; segregação; método de melhoramento |
description |
The objective of this work was to construct linkage maps of ‘Pêra’ sweet orange [Citrus sinensis (L.) Osbeck] and ‘Cravo’ mandarin (Citrus reticulata Blanco) using RAPD markers and the pseudo-testcross strategy. The parents were chosen according to the resistance/susceptibility to citrus variegate chlorosis (CVC). The segregation of 176 markers was analyzed in 94 progeny of F1 hybrids, which were obtained from controlled crossings. The linkage map of ‘Pêra’ sweet orange had 117 markers defined by 12 linkage groups, which spanned 612.1 cM. Only six markers could not be linked to the linkage group and 48.7% of the markers showed segregation distortion. The linkage map of ‘Cravo’ mandarin had 51 markers defined by 12 linkage groups, which spanned 353.3 cM. Only two markers did not link to the groups and 15.7% showed segregation distortion. The construction of linkage maps is relevant to future mapping studies of the inheritance of CVC, citrus canker and leprosis. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-02-01 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/6751 |
url |
https://seer.sct.embrapa.br/index.php/pab/article/view/6751 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/6751/3807 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
dc.source.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira; v.39, n.2, fev. 2004; 159-165 Pesquisa Agropecuária Brasileira; v.39, n.2, fev. 2004; 159-165 1678-3921 0100-104x reponame:Pesquisa Agropecuária Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Agropecuária Brasileira (Online) |
collection |
Pesquisa Agropecuária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
pab@sct.embrapa.br || sct.pab@embrapa.br |
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1793416660505329664 |