Diversity of Gluconacetobacter diazotrophicus isolated from sugarcane plants cultivated in Brazil

Detalhes bibliográficos
Autor(a) principal: Perin, Liamara
Data de Publicação: 2004
Outros Autores: Baldani, José Ivo, Reis, Veronica Massena
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/6836
Resumo: The objective of this study was to evaluate the diversity of Gluconacetobacter diazotrophicus isolates from different sugarcane (Saccharum spp.) varieties. This study was developed using 123 strains from the internal tissues of stems and roots, isolated from 80 species and hybrids of sugarcane, originated from different countries and maintained in two germoplasm collections localized in Bahia and Rio de Janeiro States. Five isolates obtained from coffee plants (Coffea arabica), two from pineapple (Ananas comosus), one from Pennisetum purpureum and 10 strains, which present different electrophoretic patterns, were used in the comparison. Cluster analysis of enzyme-linked immunosorbent assay (ELISA) results suggested that variations were not correlated with the plant species, sugarcane variety, geographic origin, parts of plants nor with sampling time. High nitrogen doses lowered the diversity of G. diazotrophicus.
id EMBRAPA-4_a687c39e2f2ed00a3f63cfc9b3d6c8da
oai_identifier_str oai:ojs.seer.sct.embrapa.br:article/6836
network_acronym_str EMBRAPA-4
network_name_str Pesquisa Agropecuária Brasileira (Online)
repository_id_str
spelling Diversity of Gluconacetobacter diazotrophicus isolated from sugarcane plants cultivated in BrazilDiversidade de Gluconacetobacter diazotrophicus isolada de plantas de cana-de-açúcar cultivadas no BrasilELISA; biological nitrogen fixation; endophytic bacteriaELISA; fixação biológica de nitrogênio; bactérias endofíticasThe objective of this study was to evaluate the diversity of Gluconacetobacter diazotrophicus isolates from different sugarcane (Saccharum spp.) varieties. This study was developed using 123 strains from the internal tissues of stems and roots, isolated from 80 species and hybrids of sugarcane, originated from different countries and maintained in two germoplasm collections localized in Bahia and Rio de Janeiro States. Five isolates obtained from coffee plants (Coffea arabica), two from pineapple (Ananas comosus), one from Pennisetum purpureum and 10 strains, which present different electrophoretic patterns, were used in the comparison. Cluster analysis of enzyme-linked immunosorbent assay (ELISA) results suggested that variations were not correlated with the plant species, sugarcane variety, geographic origin, parts of plants nor with sampling time. High nitrogen doses lowered the diversity of G. diazotrophicus.O objetivo deste trabalho foi avaliar a diversidade da população de Gluconacetobacter diazotrophicus oriunda de cultivares de cana-de-açúcar (Saccharum spp.) de diferentes regiões e bancos de germoplasma. O estudo foi realizado com 123 isolados, obtidos de folhas, colmos e raízes de 80 espécies e híbridos de cana-de-açúcar, procedentes de diferentes países e mantidos em coleções de germoplasma nos Estados da Bahia e Rio de Janeiro. Foram utilizados cinco isolados obtidos de plantas de café (Coffea arabica), dois de abacaxi (Ananas comosus) e um de Pennisetum purpureum e mais 10 estirpes com padrões eletroforéticos distintos, após o uso de enzimas comuns do metabolismo microbiano (MLEE). O agrupamento obtido por meio da técnica de imunoadsorção com enzima acoplada (ELISA) sugere que as variações expressas pelos isolados não estão relacionadas com a espécie de planta, a variedade de cana-de-açúcar, a origem geográfica, a parte da planta de onde os isolados foram obtidos e o tempo de amostragem. Altas doses de nitrogênio levaram à diminuição da diversidade de G. diazotrophicus.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraPerin, LiamaraBaldani, José IvoReis, Veronica Massena2004-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/6836Pesquisa Agropecuaria Brasileira; v.39, n.8, ago. 2004; 763-770Pesquisa Agropecuária Brasileira; v.39, n.8, ago. 2004; 763-7701678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/6836/3892info:eu-repo/semantics/openAccess2014-08-21T19:21:29Zoai:ojs.seer.sct.embrapa.br:article/6836Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-08-21T19:21:29Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Diversity of Gluconacetobacter diazotrophicus isolated from sugarcane plants cultivated in Brazil
Diversidade de Gluconacetobacter diazotrophicus isolada de plantas de cana-de-açúcar cultivadas no Brasil
title Diversity of Gluconacetobacter diazotrophicus isolated from sugarcane plants cultivated in Brazil
spellingShingle Diversity of Gluconacetobacter diazotrophicus isolated from sugarcane plants cultivated in Brazil
Perin, Liamara
ELISA; biological nitrogen fixation; endophytic bacteria
ELISA; fixação biológica de nitrogênio; bactérias endofíticas
title_short Diversity of Gluconacetobacter diazotrophicus isolated from sugarcane plants cultivated in Brazil
title_full Diversity of Gluconacetobacter diazotrophicus isolated from sugarcane plants cultivated in Brazil
title_fullStr Diversity of Gluconacetobacter diazotrophicus isolated from sugarcane plants cultivated in Brazil
title_full_unstemmed Diversity of Gluconacetobacter diazotrophicus isolated from sugarcane plants cultivated in Brazil
title_sort Diversity of Gluconacetobacter diazotrophicus isolated from sugarcane plants cultivated in Brazil
author Perin, Liamara
author_facet Perin, Liamara
Baldani, José Ivo
Reis, Veronica Massena
author_role author
author2 Baldani, José Ivo
Reis, Veronica Massena
author2_role author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Perin, Liamara
Baldani, José Ivo
Reis, Veronica Massena
dc.subject.por.fl_str_mv ELISA; biological nitrogen fixation; endophytic bacteria
ELISA; fixação biológica de nitrogênio; bactérias endofíticas
topic ELISA; biological nitrogen fixation; endophytic bacteria
ELISA; fixação biológica de nitrogênio; bactérias endofíticas
description The objective of this study was to evaluate the diversity of Gluconacetobacter diazotrophicus isolates from different sugarcane (Saccharum spp.) varieties. This study was developed using 123 strains from the internal tissues of stems and roots, isolated from 80 species and hybrids of sugarcane, originated from different countries and maintained in two germoplasm collections localized in Bahia and Rio de Janeiro States. Five isolates obtained from coffee plants (Coffea arabica), two from pineapple (Ananas comosus), one from Pennisetum purpureum and 10 strains, which present different electrophoretic patterns, were used in the comparison. Cluster analysis of enzyme-linked immunosorbent assay (ELISA) results suggested that variations were not correlated with the plant species, sugarcane variety, geographic origin, parts of plants nor with sampling time. High nitrogen doses lowered the diversity of G. diazotrophicus.
publishDate 2004
dc.date.none.fl_str_mv 2004-08-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/6836
url https://seer.sct.embrapa.br/index.php/pab/article/view/6836
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/6836/3892
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.39, n.8, ago. 2004; 763-770
Pesquisa Agropecuária Brasileira; v.39, n.8, ago. 2004; 763-770
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
_version_ 1793416666583924736

Registros relacionados