fAFLP markers to characterize three mume genotypes selected as rootstocks for peach tree

Detalhes bibliográficos
Autor(a) principal: Wickert, Ester
Data de Publicação: 2007
Outros Autores: Lemos, Eliana Gertrudes de Macedo, Pereira, Fernando Mendes, Mayer, Newton Alex
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/7757
Resumo: The objective of this work was the identification of fAFLP markers to be used in molecular characterization of three mume genotypes selected as rootstocks for peach tree. Twenty-four different fAFLP primer combinations were used and allowed the recognition of 648 markers, comprising 272 markers which were able to discriminate the three clones one from the other. These markers were used to calculate the groupment of the clones according to their similarities with the distance method and neighbour joining algorithm. The same markers were also used to calculate the genetic distance among the clones. The fAFLP markers were efficient to identify the clones, mainly by the combinations of selective primers Fam ACT/CAT, Joe AGG/CTT and Ned AGC/CAA. fAFLP markers allowed the genetic discrimination of the three mume genotypes. The greatest genetic distance was found between Rigitano cultivar and Clone 15. The fAFLP markers also showed greater genetic similarity between Clone 05 and Rigitano cultivar.
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spelling fAFLP markers to characterize three mume genotypes selected as rootstocks for peach treeMarcadores fAFLP na caracterização de três genótipos de umezeiro selecionados como porta-enxertos para pessegueiroPrunus mume; Prunus persica; molecular markersPrunus mume; Prunus persica; marcador molecularThe objective of this work was the identification of fAFLP markers to be used in molecular characterization of three mume genotypes selected as rootstocks for peach tree. Twenty-four different fAFLP primer combinations were used and allowed the recognition of 648 markers, comprising 272 markers which were able to discriminate the three clones one from the other. These markers were used to calculate the groupment of the clones according to their similarities with the distance method and neighbour joining algorithm. The same markers were also used to calculate the genetic distance among the clones. The fAFLP markers were efficient to identify the clones, mainly by the combinations of selective primers Fam ACT/CAT, Joe AGG/CTT and Ned AGC/CAA. fAFLP markers allowed the genetic discrimination of the three mume genotypes. The greatest genetic distance was found between Rigitano cultivar and Clone 15. The fAFLP markers also showed greater genetic similarity between Clone 05 and Rigitano cultivar.O objetivo deste trabalho foi caracterizar a diversidade genética existente em três genótipos de umezeiro (Clone 05, cv. Rigitano e Clone 15) e identificar marcadores moleculares fAFLP (fluorescent Amplified Fragment Lenght Polymorphism) passíveis de serem utilizados na discriminação dos três genótipos de umezeiro selecionados como porta-enxertos para pessegueiro. Foram utilizadas 24 diferentes combinações de primers seletivos fAFLP que geraram 648 marcas, das quais 272 foram diferenciadoras dos três genótipos entre si. As marcas diferenciadoras permitiram o agrupamento dos clones de umezeiro de acordo com sua similaridade através do Método da Distância e algorítmo Neighbour Joining. As mesmas marcas foram utilizadas para calcular a distância genética entre os clones. Com o uso de marcadores fAFLP foi possível discriminar os três genótipos de umezeiro entre si, destacando-se as combinações Fam ACT/CAT, Joe AGG/CTT e Ned AGC/CAA, que permitiram a diferenciação individual de cada um dos clones. A maior distância genética foi encontrada entre a cv. Rigitano e o Clone 15. Os marcadores fAFLP revelaram maior proximidade genética entre o Clone 05 e a cv. Rigitano.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraWickert, EsterLemos, Eliana Gertrudes de MacedoPereira, Fernando MendesMayer, Newton Alex2007-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/7757Pesquisa Agropecuaria Brasileira; v.42, n.12, dez. 2007; 1741-1746Pesquisa Agropecuária Brasileira; v.42, n.12, dez. 2007; 1741-17461678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/7757/4676info:eu-repo/semantics/openAccess2014-10-14T17:05:43Zoai:ojs.seer.sct.embrapa.br:article/7757Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-10-14T17:05:43Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv fAFLP markers to characterize three mume genotypes selected as rootstocks for peach tree
Marcadores fAFLP na caracterização de três genótipos de umezeiro selecionados como porta-enxertos para pessegueiro
title fAFLP markers to characterize three mume genotypes selected as rootstocks for peach tree
spellingShingle fAFLP markers to characterize three mume genotypes selected as rootstocks for peach tree
Wickert, Ester
Prunus mume; Prunus persica; molecular markers
Prunus mume; Prunus persica; marcador molecular
title_short fAFLP markers to characterize three mume genotypes selected as rootstocks for peach tree
title_full fAFLP markers to characterize three mume genotypes selected as rootstocks for peach tree
title_fullStr fAFLP markers to characterize three mume genotypes selected as rootstocks for peach tree
title_full_unstemmed fAFLP markers to characterize three mume genotypes selected as rootstocks for peach tree
title_sort fAFLP markers to characterize three mume genotypes selected as rootstocks for peach tree
author Wickert, Ester
author_facet Wickert, Ester
Lemos, Eliana Gertrudes de Macedo
Pereira, Fernando Mendes
Mayer, Newton Alex
author_role author
author2 Lemos, Eliana Gertrudes de Macedo
Pereira, Fernando Mendes
Mayer, Newton Alex
author2_role author
author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Wickert, Ester
Lemos, Eliana Gertrudes de Macedo
Pereira, Fernando Mendes
Mayer, Newton Alex
dc.subject.por.fl_str_mv Prunus mume; Prunus persica; molecular markers
Prunus mume; Prunus persica; marcador molecular
topic Prunus mume; Prunus persica; molecular markers
Prunus mume; Prunus persica; marcador molecular
description The objective of this work was the identification of fAFLP markers to be used in molecular characterization of three mume genotypes selected as rootstocks for peach tree. Twenty-four different fAFLP primer combinations were used and allowed the recognition of 648 markers, comprising 272 markers which were able to discriminate the three clones one from the other. These markers were used to calculate the groupment of the clones according to their similarities with the distance method and neighbour joining algorithm. The same markers were also used to calculate the genetic distance among the clones. The fAFLP markers were efficient to identify the clones, mainly by the combinations of selective primers Fam ACT/CAT, Joe AGG/CTT and Ned AGC/CAA. fAFLP markers allowed the genetic discrimination of the three mume genotypes. The greatest genetic distance was found between Rigitano cultivar and Clone 15. The fAFLP markers also showed greater genetic similarity between Clone 05 and Rigitano cultivar.
publishDate 2007
dc.date.none.fl_str_mv 2007-12-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/7757
url https://seer.sct.embrapa.br/index.php/pab/article/view/7757
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/7757/4676
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.42, n.12, dez. 2007; 1741-1746
Pesquisa Agropecuária Brasileira; v.42, n.12, dez. 2007; 1741-1746
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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