Identification of microsatellite markers associated with seed-coat darkening in common bean
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Pesquisa Agropecuária Brasileira (Online) |
Texto Completo: | https://seer.sct.embrapa.br/index.php/pab/article/view/8562 |
Resumo: | The objective of this work was to indentify microsatellite markers linked to the quantitative trait loci (QTL) responsible for the late seed-coat darkening in the carioca type common bean, in order to reduce the evaluation time spent in selection for this trait. Data of seed-coat darkening of 185 F2:3 progenies derived from the cross VC-3 x 'BRSMG Majestoso', were used. Out of these data, two segregating DNA bulks were constructed to evaluate 444 SSR primers. The applicatives GQMOL and Sisvar were used to evaluate the segregations, elaborate the linkage groups, and to perform single mark and regression analysis (backward method). Eight markers were polymorphic in the DNA bulks. Six of them were mapped in a 80.49-cM linkage group. Out of these six markers, three were very close to the QTL responsible for the late grain darkening. The PVM02TC116 marker co-segregate with that QTL, and the PVESTBR-98 (2.00 cM) and PV176 (12.24 cM) ones flank it, which indicates that high efficiency in the assisted selection is possible. |
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Identification of microsatellite markers associated with seed-coat darkening in common beanIdentificação de marcadores microssatélites relacionados ao escurecimento de grãos em feijãoPhaseolus vulgaris; quantitative trait loci; molecular marker; assisted selection; SSRPhaseolus vulgaris; locos de características quantitativas; marcador molecular; seleção assistida; SSRThe objective of this work was to indentify microsatellite markers linked to the quantitative trait loci (QTL) responsible for the late seed-coat darkening in the carioca type common bean, in order to reduce the evaluation time spent in selection for this trait. Data of seed-coat darkening of 185 F2:3 progenies derived from the cross VC-3 x 'BRSMG Majestoso', were used. Out of these data, two segregating DNA bulks were constructed to evaluate 444 SSR primers. The applicatives GQMOL and Sisvar were used to evaluate the segregations, elaborate the linkage groups, and to perform single mark and regression analysis (backward method). Eight markers were polymorphic in the DNA bulks. Six of them were mapped in a 80.49-cM linkage group. Out of these six markers, three were very close to the QTL responsible for the late grain darkening. The PVM02TC116 marker co-segregate with that QTL, and the PVESTBR-98 (2.00 cM) and PV176 (12.24 cM) ones flank it, which indicates that high efficiency in the assisted selection is possible.O objetivo deste trabalho foi identificar marcadores microssatélites ligados ao loco de características quantitativas (QTL) responsável pelo escurecimento tardio do tegumento de feijões do tipo carioca, a fim de reduzir o tempo de avaliação necessário para seleção quanto a essa característica. Foram utilizados dados de avaliação fenotípica de 185 progênies F2:3 derivadas do cruzamento VC-3 x 'BRSMG Majestoso', para o estudo do controle genético do escurecimento dos grãos. Com esses dados, foram confeccionados dois "bulks" segregantes de DNA, empregados para a avaliação de 444 pares de primers SSR. Os aplicativos computacionais GQMOL e Sisvar foram utilizados para avaliar as segregações, confeccionar um grupo de ligação e realizar análises de marca simples e de regressão múltipla pelo método "backward". Oito marcadores apresentaram polimorfismo nos "bulks". Seis desses marcadores foram agrupados em um grupo de ligação de 80,49 cM, e destes, três mostraram-se estreitamente ligados ao QTL responsável pelo escurecimento tardio dos grãos. O marcador PVM02TC116 cossegregou com o QTL em questão, e os marcadores PVESTBR-98 (2,00 cM) e PV176 (12,24 cM) flanqueiam essa região, o que sugere elevada eficiência para possível uso na seleção assistida. Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraCNPqCouto, Karla RodriguesSantos, João Bosco dosRamalho, Magno Antônio PattoSilva, Graciele Simoneti da2011-01-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/8562Pesquisa Agropecuaria Brasileira; v.45, n.11, nov. 2010; 1268-1274Pesquisa Agropecuária Brasileira; v.45, n.11, nov. 2010; 1268-12741678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/8562/6096info:eu-repo/semantics/openAccess2014-11-13T17:09:39Zoai:ojs.seer.sct.embrapa.br:article/8562Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-11-13T17:09:39Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Identification of microsatellite markers associated with seed-coat darkening in common bean Identificação de marcadores microssatélites relacionados ao escurecimento de grãos em feijão |
title |
Identification of microsatellite markers associated with seed-coat darkening in common bean |
spellingShingle |
Identification of microsatellite markers associated with seed-coat darkening in common bean Couto, Karla Rodrigues Phaseolus vulgaris; quantitative trait loci; molecular marker; assisted selection; SSR Phaseolus vulgaris; locos de características quantitativas; marcador molecular; seleção assistida; SSR |
title_short |
Identification of microsatellite markers associated with seed-coat darkening in common bean |
title_full |
Identification of microsatellite markers associated with seed-coat darkening in common bean |
title_fullStr |
Identification of microsatellite markers associated with seed-coat darkening in common bean |
title_full_unstemmed |
Identification of microsatellite markers associated with seed-coat darkening in common bean |
title_sort |
Identification of microsatellite markers associated with seed-coat darkening in common bean |
author |
Couto, Karla Rodrigues |
author_facet |
Couto, Karla Rodrigues Santos, João Bosco dos Ramalho, Magno Antônio Patto Silva, Graciele Simoneti da |
author_role |
author |
author2 |
Santos, João Bosco dos Ramalho, Magno Antônio Patto Silva, Graciele Simoneti da |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
CNPq |
dc.contributor.author.fl_str_mv |
Couto, Karla Rodrigues Santos, João Bosco dos Ramalho, Magno Antônio Patto Silva, Graciele Simoneti da |
dc.subject.por.fl_str_mv |
Phaseolus vulgaris; quantitative trait loci; molecular marker; assisted selection; SSR Phaseolus vulgaris; locos de características quantitativas; marcador molecular; seleção assistida; SSR |
topic |
Phaseolus vulgaris; quantitative trait loci; molecular marker; assisted selection; SSR Phaseolus vulgaris; locos de características quantitativas; marcador molecular; seleção assistida; SSR |
description |
The objective of this work was to indentify microsatellite markers linked to the quantitative trait loci (QTL) responsible for the late seed-coat darkening in the carioca type common bean, in order to reduce the evaluation time spent in selection for this trait. Data of seed-coat darkening of 185 F2:3 progenies derived from the cross VC-3 x 'BRSMG Majestoso', were used. Out of these data, two segregating DNA bulks were constructed to evaluate 444 SSR primers. The applicatives GQMOL and Sisvar were used to evaluate the segregations, elaborate the linkage groups, and to perform single mark and regression analysis (backward method). Eight markers were polymorphic in the DNA bulks. Six of them were mapped in a 80.49-cM linkage group. Out of these six markers, three were very close to the QTL responsible for the late grain darkening. The PVM02TC116 marker co-segregate with that QTL, and the PVESTBR-98 (2.00 cM) and PV176 (12.24 cM) ones flank it, which indicates that high efficiency in the assisted selection is possible. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-01-28 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/8562 |
url |
https://seer.sct.embrapa.br/index.php/pab/article/view/8562 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/8562/6096 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
dc.source.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira; v.45, n.11, nov. 2010; 1268-1274 Pesquisa Agropecuária Brasileira; v.45, n.11, nov. 2010; 1268-1274 1678-3921 0100-104x reponame:Pesquisa Agropecuária Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Agropecuária Brasileira (Online) |
collection |
Pesquisa Agropecuária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
pab@sct.embrapa.br || sct.pab@embrapa.br |
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1793416666560856064 |