Comparison of procedures for nitrate determination in vegetable tissue

Detalhes bibliográficos
Autor(a) principal: Mantovani, José Ricardo
Data de Publicação: 2005
Outros Autores: Cruz, Mara Cristina Pessôa da, Ferreira, Manoel Evaristo, Barbosa, José Carlos
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/6921
Resumo: Nitrate content determination is important for food quality evaluation, but its determination is affected by interferences. The objective of this work was to compare nitrate determination procedures for vegetable tissues. In dry matter samples from three lettuce cultivars that received five levels of nitrogen in a greenhouse experiment, nitrate was extracted with deionized water and its determination was made by the cadmium column method, the distillation method, the salicylic acid method, and the method using the reductive mixture with zinc. The salicylic acid method and that of reductive mixture with zinc overestimated the nitrate content in lettuce dry matter, because they were influenced by the color of dry matter extracts and other interferences. The cadmium column and the distillation methods are the best for nitrate determination in vegetable tissues, but the simplicity and lower cost of distillation in relation to cadmium column method suggest that the first one must be recommended.
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spelling Comparison of procedures for nitrate determination in vegetable tissueComparação de procedimentos de quantificação de nitrato em tecido vegetalLactuca sativa; plant analysis; nitrogenLactuca sativa; análise de plantas; nitrogênioNitrate content determination is important for food quality evaluation, but its determination is affected by interferences. The objective of this work was to compare nitrate determination procedures for vegetable tissues. In dry matter samples from three lettuce cultivars that received five levels of nitrogen in a greenhouse experiment, nitrate was extracted with deionized water and its determination was made by the cadmium column method, the distillation method, the salicylic acid method, and the method using the reductive mixture with zinc. The salicylic acid method and that of reductive mixture with zinc overestimated the nitrate content in lettuce dry matter, because they were influenced by the color of dry matter extracts and other interferences. The cadmium column and the distillation methods are the best for nitrate determination in vegetable tissues, but the simplicity and lower cost of distillation in relation to cadmium column method suggest that the first one must be recommended.O teor de nitrato é um importante índice da qualidade dos alimentos, mas existem problemas na sua quantificação. O objetivo deste trabalho foi comparar procedimentos de quantificação de nitrato em tecido vegetal. Em amostras de matéria seca da parte aérea de três cultivares de alface adubadas com cinco doses de nitrogênio, provenientes de experimento realizado em casa de vegetação, foi feita extração de nitrato com água desionizada e quantificação, utilizando os procedimentos da coluna redutora contendo cádmio, da destilação, do ácido salicílico e da mistura redutora contendo zinco. Os procedimentos do ácido salicílico e da mistura redutora contendo zinco superestimam os teores de nitrato na matéria seca de alface, pois são mais sujeitos à presença de interferentes e ao efeito da cor do extrato. Os procedimentos da coluna redutora contendo cádmio e o da destilação são os mais adequados na quantificação de nitrato em tecido vegetal. Contudo, a simplicidade e o menor custo da destilação em relação à coluna redutora indicam que a destilação deve ser recomendada.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraMantovani, José RicardoCruz, Mara Cristina Pessôa daFerreira, Manoel EvaristoBarbosa, José Carlos2005-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/6921Pesquisa Agropecuaria Brasileira; v.40, n.1, jan. 2005; 53-59Pesquisa Agropecuária Brasileira; v.40, n.1, jan. 2005; 53-591678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/6921/3969info:eu-repo/semantics/openAccess2014-09-23T18:33:24Zoai:ojs.seer.sct.embrapa.br:article/6921Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-09-23T18:33:24Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Comparison of procedures for nitrate determination in vegetable tissue
Comparação de procedimentos de quantificação de nitrato em tecido vegetal
title Comparison of procedures for nitrate determination in vegetable tissue
spellingShingle Comparison of procedures for nitrate determination in vegetable tissue
Mantovani, José Ricardo
Lactuca sativa; plant analysis; nitrogen
Lactuca sativa; análise de plantas; nitrogênio
title_short Comparison of procedures for nitrate determination in vegetable tissue
title_full Comparison of procedures for nitrate determination in vegetable tissue
title_fullStr Comparison of procedures for nitrate determination in vegetable tissue
title_full_unstemmed Comparison of procedures for nitrate determination in vegetable tissue
title_sort Comparison of procedures for nitrate determination in vegetable tissue
author Mantovani, José Ricardo
author_facet Mantovani, José Ricardo
Cruz, Mara Cristina Pessôa da
Ferreira, Manoel Evaristo
Barbosa, José Carlos
author_role author
author2 Cruz, Mara Cristina Pessôa da
Ferreira, Manoel Evaristo
Barbosa, José Carlos
author2_role author
author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Mantovani, José Ricardo
Cruz, Mara Cristina Pessôa da
Ferreira, Manoel Evaristo
Barbosa, José Carlos
dc.subject.por.fl_str_mv Lactuca sativa; plant analysis; nitrogen
Lactuca sativa; análise de plantas; nitrogênio
topic Lactuca sativa; plant analysis; nitrogen
Lactuca sativa; análise de plantas; nitrogênio
description Nitrate content determination is important for food quality evaluation, but its determination is affected by interferences. The objective of this work was to compare nitrate determination procedures for vegetable tissues. In dry matter samples from three lettuce cultivars that received five levels of nitrogen in a greenhouse experiment, nitrate was extracted with deionized water and its determination was made by the cadmium column method, the distillation method, the salicylic acid method, and the method using the reductive mixture with zinc. The salicylic acid method and that of reductive mixture with zinc overestimated the nitrate content in lettuce dry matter, because they were influenced by the color of dry matter extracts and other interferences. The cadmium column and the distillation methods are the best for nitrate determination in vegetable tissues, but the simplicity and lower cost of distillation in relation to cadmium column method suggest that the first one must be recommended.
publishDate 2005
dc.date.none.fl_str_mv 2005-01-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/6921
url https://seer.sct.embrapa.br/index.php/pab/article/view/6921
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/6921/3969
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.40, n.1, jan. 2005; 53-59
Pesquisa Agropecuária Brasileira; v.40, n.1, jan. 2005; 53-59
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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