Production of androgenetic barley doubled haploid lines

Detalhes bibliográficos
Autor(a) principal: Silva, Ana Lúcia Stival da
Data de Publicação: 1997
Outros Autores: Fernandes, Maria Irene Baggio de Moraes, Arias, Gerardo, Ferreira, Alfredo Gui, Haas, João Carlos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/4762
Resumo: Anther culture allows obtention of homozygous lines in one generation instead of seven required by conventional breeding programs. In barley (Hordeum vulgare L.) this technique is well-established, but response is greatly influenced by genotype and growing conditions of donor plants. Therefore, an important goal is to adapt media and methods, aiming to satisfy the specific requirements of every material one has been working with. In this paper androgenetic capacity of F1 hybrids is evaluated and an efficient protocol for regeneration of barley doubled haploids is established in order to make possible their utilization in breeding programs. Two culture media, modified N6 and MS, were employed for induction of androgenesis. Frequency of responsive anthers, green plant regeneration, albinism, and spontaneous doubling were evaluated. Results show that average percentage of responsive anthers was greater in N6 (30.32%) than in MS (6.39%) medium. There was a considerable influence of the genotype for all traits. A total of 192 doubled haploid lines were obtained from different crosses. These lines set seed and were multiplied for agronomic testing in the field.
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spelling Production of androgenetic barley doubled haploid linesProdução de linhagens duplo-haplóides androgenéticas de cevadaanther culturecultura de anterasAnther culture allows obtention of homozygous lines in one generation instead of seven required by conventional breeding programs. In barley (Hordeum vulgare L.) this technique is well-established, but response is greatly influenced by genotype and growing conditions of donor plants. Therefore, an important goal is to adapt media and methods, aiming to satisfy the specific requirements of every material one has been working with. In this paper androgenetic capacity of F1 hybrids is evaluated and an efficient protocol for regeneration of barley doubled haploids is established in order to make possible their utilization in breeding programs. Two culture media, modified N6 and MS, were employed for induction of androgenesis. Frequency of responsive anthers, green plant regeneration, albinism, and spontaneous doubling were evaluated. Results show that average percentage of responsive anthers was greater in N6 (30.32%) than in MS (6.39%) medium. There was a considerable influence of the genotype for all traits. A total of 192 doubled haploid lines were obtained from different crosses. These lines set seed and were multiplied for agronomic testing in the field.A cultura de anteras permite a obtenção de linhagens homozigotas em uma geração, em vez das sete requeridas pelos programas convencionais de melhoramento. Essa técnica encontra-se bem estabelecida com respeito a cevada (Hordeum vulgare L.), embora a resposta seja muito influenciada pelo genótipo e pelas condições de cultivo das plantas doadoras. Por isso, torna-se importante otimizar meios e métodos, buscando satisfazer os requerimentos específicos de cada material usado no trabalho. O objetivo deste trabalho foi avaliar a capacidade androgenética de híbridos F1 de cevada e estabelecer um protocolo eficiente de regeneração de duplo-haplóides, para tornar possível sua utilização em programas de melhoramento. Dois meios de cultura, N6 e MS modificados, foram utilizados para indução de androgênese. Avaliou-se a freqüência de anteras responsivas e de regeneração de plantas verdes, e a porcentagem de albinismo e de duplicação espontânea. Os resultados mostram que a porcentagem média de anteras responsivas no meio N6 (30,32%) foi maior do que no meio MS (6,39%). Houve uma considerável influência do genótipo em relação a todas as variáveis analisadas. No total, 192 genótipos duplo-haplóides, originados de diferentes cruzamentos, foram cultivados até a produção de grãos. As sementes de cada genótipo foram multiplicadas, para avaliação agronômica.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraSilva, Ana Lúcia Stival daFernandes, Maria Irene Baggio de MoraesArias, GerardoFerreira, Alfredo GuiHaas, João Carlos1997-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/4762Pesquisa Agropecuaria Brasileira; v.32, n.11, nov. 1997; 1159-1166Pesquisa Agropecuária Brasileira; v.32, n.11, nov. 1997; 1159-11661678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/4762/7270info:eu-repo/semantics/openAccess2015-04-14T16:27:27Zoai:ojs.seer.sct.embrapa.br:article/4762Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2015-04-14T16:27:27Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Production of androgenetic barley doubled haploid lines
Produção de linhagens duplo-haplóides androgenéticas de cevada
title Production of androgenetic barley doubled haploid lines
spellingShingle Production of androgenetic barley doubled haploid lines
Silva, Ana Lúcia Stival da
anther culture
cultura de anteras
title_short Production of androgenetic barley doubled haploid lines
title_full Production of androgenetic barley doubled haploid lines
title_fullStr Production of androgenetic barley doubled haploid lines
title_full_unstemmed Production of androgenetic barley doubled haploid lines
title_sort Production of androgenetic barley doubled haploid lines
author Silva, Ana Lúcia Stival da
author_facet Silva, Ana Lúcia Stival da
Fernandes, Maria Irene Baggio de Moraes
Arias, Gerardo
Ferreira, Alfredo Gui
Haas, João Carlos
author_role author
author2 Fernandes, Maria Irene Baggio de Moraes
Arias, Gerardo
Ferreira, Alfredo Gui
Haas, João Carlos
author2_role author
author
author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Silva, Ana Lúcia Stival da
Fernandes, Maria Irene Baggio de Moraes
Arias, Gerardo
Ferreira, Alfredo Gui
Haas, João Carlos
dc.subject.por.fl_str_mv anther culture
cultura de anteras
topic anther culture
cultura de anteras
description Anther culture allows obtention of homozygous lines in one generation instead of seven required by conventional breeding programs. In barley (Hordeum vulgare L.) this technique is well-established, but response is greatly influenced by genotype and growing conditions of donor plants. Therefore, an important goal is to adapt media and methods, aiming to satisfy the specific requirements of every material one has been working with. In this paper androgenetic capacity of F1 hybrids is evaluated and an efficient protocol for regeneration of barley doubled haploids is established in order to make possible their utilization in breeding programs. Two culture media, modified N6 and MS, were employed for induction of androgenesis. Frequency of responsive anthers, green plant regeneration, albinism, and spontaneous doubling were evaluated. Results show that average percentage of responsive anthers was greater in N6 (30.32%) than in MS (6.39%) medium. There was a considerable influence of the genotype for all traits. A total of 192 doubled haploid lines were obtained from different crosses. These lines set seed and were multiplied for agronomic testing in the field.
publishDate 1997
dc.date.none.fl_str_mv 1997-11-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/4762
url https://seer.sct.embrapa.br/index.php/pab/article/view/4762
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/4762/7270
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.32, n.11, nov. 1997; 1159-1166
Pesquisa Agropecuária Brasileira; v.32, n.11, nov. 1997; 1159-1166
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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