Enzymatic activity in sugar cane varieties cultivated in vitro under nitrogen levels

Detalhes bibliográficos
Autor(a) principal: Donato, Virgínia Maria Tenório Sabino
Data de Publicação: 2004
Outros Autores: Andrade, Arnóbio Gonçalves de, Souza, Eidy Simões de, França, José Geraldo Eugênio de, Maciel, Gabriel Alves
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/6881
Resumo: Nitrogen is considered the most abundant mineral element in the plants and an essential component to biomolecules and several enzymes. The objective of this work was to estimate nitrogen assimilation efficiency process and to identify biological fixation potential parameters on sugar cane varieties cultivated in vitro. The sugar cane varieties used were RB 842021, RB 83102, RB 75126, RB 882980, CO 997, besides Brachiaria arrecta (witness), cultivated in vitro in different nitrogen levels [M1 (9.83 mM), M2 (2.46 mM), M3 (0.49 mM), M4 (0.0 mM)]. All the varieties and plant witness (Brachiaria arrecta) exhibited redutase nitrate activity and glutamina sintetase, even in the ammonium and nitrate absence culture media. The RB 842021 variety showed the highest activity of nitrate reductase and the higher chlorophyll a and b contents, whereas RB 882980 variety showed the highest glutamine sintetase activity when cultivated without nitrogen, representing a higher potential assimilation of nitrogen.
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spelling Enzymatic activity in sugar cane varieties cultivated in vitro under nitrogen levelsAtividade enzimática em variedades de cana-de-açúcar cultivadas in vitro sob diferentes níveis de nitrogênioSaccharum; glutamina sintetase; nitrate reductase; chlorophyllSaccharum; glutamina sintetase; redutase do nitrato; clorofilaNitrogen is considered the most abundant mineral element in the plants and an essential component to biomolecules and several enzymes. The objective of this work was to estimate nitrogen assimilation efficiency process and to identify biological fixation potential parameters on sugar cane varieties cultivated in vitro. The sugar cane varieties used were RB 842021, RB 83102, RB 75126, RB 882980, CO 997, besides Brachiaria arrecta (witness), cultivated in vitro in different nitrogen levels [M1 (9.83 mM), M2 (2.46 mM), M3 (0.49 mM), M4 (0.0 mM)]. All the varieties and plant witness (Brachiaria arrecta) exhibited redutase nitrate activity and glutamina sintetase, even in the ammonium and nitrate absence culture media. The RB 842021 variety showed the highest activity of nitrate reductase and the higher chlorophyll a and b contents, whereas RB 882980 variety showed the highest glutamine sintetase activity when cultivated without nitrogen, representing a higher potential assimilation of nitrogen.O nitrogênio é considerado o elemento mineral mais abundante nas plantas, sendo componente essencial de biomoléculas e inúmeras enzimas. O objetivo deste trabalho foi avaliar a eficiência no processo de assimilação do nitrogênio e encontrar parâmetros indicativos do potencial de fixação biológica em variedades de cana-de-açúcar (Saccharum officinarum) cultivadas in vitro. Foram utilizadas as variedades de cana-de-açúcar RB 842021, RB 83102, RB 75126, RB 882980 e Co 997, além da Brachiaria arrecta (testemunha), cultivadas in vitro em diferentes níveis de nitrogênio [M1 (9,83 mM), M2 (2,46 mM), M3 (0,49 mM), M4 (0,0 mM)]. Todas as variedades e a cultura testemunha (Brachiaria arrecta) apresentaram atividade da nitrato redutase (NR) constitutiva e também da glutamina sintetase (GS), mesmo na ausência de amônio e nitrato no meio de cultura. A variedade RB 842021 apresentou a maior atividade da nitrato redutase e o maior conteúdo de clorofilas a e b, e a variedade RB 882980 a maior atividade da glutamina sintetase nas mesmas condições de cultivo, o que pode representar maior potencial de assimilação do nitrogênio.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraDonato, Virgínia Maria Tenório SabinoAndrade, Arnóbio Gonçalves deSouza, Eidy Simões deFrança, José Geraldo Eugênio deMaciel, Gabriel Alves2004-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/6881Pesquisa Agropecuaria Brasileira; v.39, n.11, nov. 2004; 1087-1093Pesquisa Agropecuária Brasileira; v.39, n.11, nov. 2004; 1087-10931678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/6881/3937info:eu-repo/semantics/openAccess2014-09-03T16:35:14Zoai:ojs.seer.sct.embrapa.br:article/6881Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-09-03T16:35:14Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Enzymatic activity in sugar cane varieties cultivated in vitro under nitrogen levels
Atividade enzimática em variedades de cana-de-açúcar cultivadas in vitro sob diferentes níveis de nitrogênio
title Enzymatic activity in sugar cane varieties cultivated in vitro under nitrogen levels
spellingShingle Enzymatic activity in sugar cane varieties cultivated in vitro under nitrogen levels
Donato, Virgínia Maria Tenório Sabino
Saccharum; glutamina sintetase; nitrate reductase; chlorophyll
Saccharum; glutamina sintetase; redutase do nitrato; clorofila
title_short Enzymatic activity in sugar cane varieties cultivated in vitro under nitrogen levels
title_full Enzymatic activity in sugar cane varieties cultivated in vitro under nitrogen levels
title_fullStr Enzymatic activity in sugar cane varieties cultivated in vitro under nitrogen levels
title_full_unstemmed Enzymatic activity in sugar cane varieties cultivated in vitro under nitrogen levels
title_sort Enzymatic activity in sugar cane varieties cultivated in vitro under nitrogen levels
author Donato, Virgínia Maria Tenório Sabino
author_facet Donato, Virgínia Maria Tenório Sabino
Andrade, Arnóbio Gonçalves de
Souza, Eidy Simões de
França, José Geraldo Eugênio de
Maciel, Gabriel Alves
author_role author
author2 Andrade, Arnóbio Gonçalves de
Souza, Eidy Simões de
França, José Geraldo Eugênio de
Maciel, Gabriel Alves
author2_role author
author
author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Donato, Virgínia Maria Tenório Sabino
Andrade, Arnóbio Gonçalves de
Souza, Eidy Simões de
França, José Geraldo Eugênio de
Maciel, Gabriel Alves
dc.subject.por.fl_str_mv Saccharum; glutamina sintetase; nitrate reductase; chlorophyll
Saccharum; glutamina sintetase; redutase do nitrato; clorofila
topic Saccharum; glutamina sintetase; nitrate reductase; chlorophyll
Saccharum; glutamina sintetase; redutase do nitrato; clorofila
description Nitrogen is considered the most abundant mineral element in the plants and an essential component to biomolecules and several enzymes. The objective of this work was to estimate nitrogen assimilation efficiency process and to identify biological fixation potential parameters on sugar cane varieties cultivated in vitro. The sugar cane varieties used were RB 842021, RB 83102, RB 75126, RB 882980, CO 997, besides Brachiaria arrecta (witness), cultivated in vitro in different nitrogen levels [M1 (9.83 mM), M2 (2.46 mM), M3 (0.49 mM), M4 (0.0 mM)]. All the varieties and plant witness (Brachiaria arrecta) exhibited redutase nitrate activity and glutamina sintetase, even in the ammonium and nitrate absence culture media. The RB 842021 variety showed the highest activity of nitrate reductase and the higher chlorophyll a and b contents, whereas RB 882980 variety showed the highest glutamine sintetase activity when cultivated without nitrogen, representing a higher potential assimilation of nitrogen.
publishDate 2004
dc.date.none.fl_str_mv 2004-11-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/6881
url https://seer.sct.embrapa.br/index.php/pab/article/view/6881
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/6881/3937
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.39, n.11, nov. 2004; 1087-1093
Pesquisa Agropecuária Brasileira; v.39, n.11, nov. 2004; 1087-1093
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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