Characterization of rue extract and its potential for controlling rice blast

Detalhes bibliográficos
Autor(a) principal: Reis, Karinna Bannach
Data de Publicação: 2015
Outros Autores: Côrtes, Marcio Vinicius de Carvalho Barros, Martins, Frederico Severino, Filippi, Marta Cristina Corsi de, Paula, José Realino de, Conceição, Edemilson Cardoso da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/21451
Resumo: The objective of this work was to purify and standardize the rue (Ruta graveolens) extract and evaluate its effect on Magnaporthe oryzae as an alternative to the integrated management of rice blast. The drug was characterized, the liquid extract was obtained, and the methodology for quantifying the standard markers psoralen and bergapten was validated. Rue extract and the markers, solely or in combination, were assayed in vitro, as well as in greenhouse conditions, for their ability to suppress leaf blast, by the evaluation of mycelial growth, conidial germination, and appressorium formation. Rue extract inhibited M. oryzae mycelial growth (100%), conidial germination (LD50=0.237 mg), and the appressorium formation (LD50=0.121 mg); besides, the extract reduced leaf blast severity by 80.84%. Fluorescence microscopy showed that rue extract did not damage M. oryzae cell wall and plasma membrane, indicating another mode of action. Rue extract has a great potential for controlling rice leaf blast.
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spelling Characterization of rue extract and its potential for controlling rice blastCaracterização do extrato de arruda e avaliação de seu potencial para o controle da brusoneMagnaporthe oryzae; Ruta graveolens; bergapten; psoralenMagnaporthe oryzae; Ruta graveolens; bergapteno; psoralenoThe objective of this work was to purify and standardize the rue (Ruta graveolens) extract and evaluate its effect on Magnaporthe oryzae as an alternative to the integrated management of rice blast. The drug was characterized, the liquid extract was obtained, and the methodology for quantifying the standard markers psoralen and bergapten was validated. Rue extract and the markers, solely or in combination, were assayed in vitro, as well as in greenhouse conditions, for their ability to suppress leaf blast, by the evaluation of mycelial growth, conidial germination, and appressorium formation. Rue extract inhibited M. oryzae mycelial growth (100%), conidial germination (LD50=0.237 mg), and the appressorium formation (LD50=0.121 mg); besides, the extract reduced leaf blast severity by 80.84%. Fluorescence microscopy showed that rue extract did not damage M. oryzae cell wall and plasma membrane, indicating another mode of action. Rue extract has a great potential for controlling rice leaf blast.O objetivo deste trabalho foi purificar e padronizar o extrato de arruda (Ruta graveolens) e avaliar o seu efeito sobre Magnaporthe oryzae como alternativa para a gestão integrada da brusone. A droga vegetal foi caracterizada, o extrato líquido obtido, e a metodologia para quantificar os marcadores padrão psoraleno e bergapteno validada. O extrato de arruda e os marcadores, isolados ou combinados, foram ensaiados in vitro, assim como em condições de casa de vegetação, para a avaliação de sua capacidade de suprimir a brusone, pela avaliação do crescimento micelial, da germinação de conídios e da formação de apressório. O extrato de arruda inibiu o crescimento micelial de M. oryzae (100%), a germinação de conídios (DL50=0,237 mg) e a formação de apressórios (DL50=0,121 mg); além disso, o extrato reduziu a severidade da brusone em 80,84%. A microscopia de fluorescência mostrou que o extrato de arruda não danificou a parede celular e a membrana plasmática de M. oryzae, o que indica outro modo de ação. O extrato de arruda apresenta grande potencial para o controle da brusone em folhas de arroz.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraCoordenação de Aperfeiçoamento de Pessoal de Nível Superior and Conselho Nacional de Desenvolvimento Científico e TecnológicoCoordenação de Aperfeiçoamento de Pessoal de Nível Superior and Conselho Nacional de Desenvolvimento Científico e TecnológicoReis, Karinna BannachCôrtes, Marcio Vinicius de Carvalho BarrosMartins, Frederico SeverinoFilippi, Marta Cristina Corsi dePaula, José Realino deConceição, Edemilson Cardoso da2015-12-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/21451Pesquisa Agropecuaria Brasileira; v.50, n.12, dez. 2015; 1121-1130Pesquisa Agropecuária Brasileira; v.50, n.12, dez. 2015; 1121-11301678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/21451/13127https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/21451/13564Direitos autorais 2015 Pesquisa Agropecuária Brasileirainfo:eu-repo/semantics/openAccess2015-12-23T17:41:00Zoai:ojs.seer.sct.embrapa.br:article/21451Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2015-12-23T17:41Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Characterization of rue extract and its potential for controlling rice blast
Caracterização do extrato de arruda e avaliação de seu potencial para o controle da brusone
title Characterization of rue extract and its potential for controlling rice blast
spellingShingle Characterization of rue extract and its potential for controlling rice blast
Reis, Karinna Bannach
Magnaporthe oryzae; Ruta graveolens; bergapten; psoralen
Magnaporthe oryzae; Ruta graveolens; bergapteno; psoraleno
title_short Characterization of rue extract and its potential for controlling rice blast
title_full Characterization of rue extract and its potential for controlling rice blast
title_fullStr Characterization of rue extract and its potential for controlling rice blast
title_full_unstemmed Characterization of rue extract and its potential for controlling rice blast
title_sort Characterization of rue extract and its potential for controlling rice blast
author Reis, Karinna Bannach
author_facet Reis, Karinna Bannach
Côrtes, Marcio Vinicius de Carvalho Barros
Martins, Frederico Severino
Filippi, Marta Cristina Corsi de
Paula, José Realino de
Conceição, Edemilson Cardoso da
author_role author
author2 Côrtes, Marcio Vinicius de Carvalho Barros
Martins, Frederico Severino
Filippi, Marta Cristina Corsi de
Paula, José Realino de
Conceição, Edemilson Cardoso da
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Coordenação de Aperfeiçoamento de Pessoal de Nível Superior and Conselho Nacional de Desenvolvimento Científico e Tecnológico
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior and Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.contributor.author.fl_str_mv Reis, Karinna Bannach
Côrtes, Marcio Vinicius de Carvalho Barros
Martins, Frederico Severino
Filippi, Marta Cristina Corsi de
Paula, José Realino de
Conceição, Edemilson Cardoso da
dc.subject.por.fl_str_mv Magnaporthe oryzae; Ruta graveolens; bergapten; psoralen
Magnaporthe oryzae; Ruta graveolens; bergapteno; psoraleno
topic Magnaporthe oryzae; Ruta graveolens; bergapten; psoralen
Magnaporthe oryzae; Ruta graveolens; bergapteno; psoraleno
description The objective of this work was to purify and standardize the rue (Ruta graveolens) extract and evaluate its effect on Magnaporthe oryzae as an alternative to the integrated management of rice blast. The drug was characterized, the liquid extract was obtained, and the methodology for quantifying the standard markers psoralen and bergapten was validated. Rue extract and the markers, solely or in combination, were assayed in vitro, as well as in greenhouse conditions, for their ability to suppress leaf blast, by the evaluation of mycelial growth, conidial germination, and appressorium formation. Rue extract inhibited M. oryzae mycelial growth (100%), conidial germination (LD50=0.237 mg), and the appressorium formation (LD50=0.121 mg); besides, the extract reduced leaf blast severity by 80.84%. Fluorescence microscopy showed that rue extract did not damage M. oryzae cell wall and plasma membrane, indicating another mode of action. Rue extract has a great potential for controlling rice leaf blast.
publishDate 2015
dc.date.none.fl_str_mv 2015-12-23
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/21451
url https://seer.sct.embrapa.br/index.php/pab/article/view/21451
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/21451/13127
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/21451/13564
dc.rights.driver.fl_str_mv Direitos autorais 2015 Pesquisa Agropecuária Brasileira
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Direitos autorais 2015 Pesquisa Agropecuária Brasileira
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.50, n.12, dez. 2015; 1121-1130
Pesquisa Agropecuária Brasileira; v.50, n.12, dez. 2015; 1121-1130
1678-3921
0100-104x
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institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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