Characterization of rue extract and its potential for controlling rice blast.

Detalhes bibliográficos
Autor(a) principal: REIS, K. B.
Data de Publicação: 2015
Outros Autores: CORTÊS, M. V. de C. B., MARTINS, F. S., FILIPPI, M. C. C. de, PAULA, J. R. de, CONCEIÇÃO, E. C. da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1031992
Resumo: The objective of this work was to purify and standardize the extract of rue (Ruta graveolens) and evaluate its effect on Magnaporthe oryzae as an alternative to the integrated management of rice blast. The drug was characterized, the liquid extract was obtained, and the methodology for quantifying the standard markers furanocoumarins psoralen and bergapten was validated. Rue extract and the markers, solely or in combination, were assayed in vitro, as well as in greenhouse conditions, for their ability to suppress leaf blast, by the evaluation of mycelial growth, conidial germination, and appressorium formation. Rue extract inhibited M. oryzae mycelial growth (100%), conidial germination (LD50=0.237 mg), and the appressorium formation (LD50=0.121 mg); besides, the extract reduced leaf blast by 80.84%. Fluorescence microscopy showed that rue extract did not damage M. oryzae cell wall and plasma membrane, indicating another mode of action. Rue extract has a great potential for controlling rice leaf blast.
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spelling Characterization of rue extract and its potential for controlling rice blast.BergaptenoPsoralenoControleArrozArrudaRuta graveolensBrusoneMagnaporthe oryzaeThe objective of this work was to purify and standardize the extract of rue (Ruta graveolens) and evaluate its effect on Magnaporthe oryzae as an alternative to the integrated management of rice blast. The drug was characterized, the liquid extract was obtained, and the methodology for quantifying the standard markers furanocoumarins psoralen and bergapten was validated. Rue extract and the markers, solely or in combination, were assayed in vitro, as well as in greenhouse conditions, for their ability to suppress leaf blast, by the evaluation of mycelial growth, conidial germination, and appressorium formation. Rue extract inhibited M. oryzae mycelial growth (100%), conidial germination (LD50=0.237 mg), and the appressorium formation (LD50=0.121 mg); besides, the extract reduced leaf blast by 80.84%. Fluorescence microscopy showed that rue extract did not damage M. oryzae cell wall and plasma membrane, indicating another mode of action. Rue extract has a great potential for controlling rice leaf blast.KARINNA BANNACH REIS, UFG; MARCIO VINICIUS DE C BARROS CORTES, CNPAF; FREDERICO SEVERINO MARTINS, USP, Ribeirão Preto-SP; MARTA CRISTINA CORSI DE FILIPPI, CNPAF; JOSE REALINO DE PAULA, UFG; EDEMILSON CARDOSO DA CONCEIÇÃO, UFG.REIS, K. B.CORTÊS, M. V. de C. B.MARTINS, F. S.FILIPPI, M. C. C. dePAULA, J. R. deCONCEIÇÃO, E. C. da2015-12-18T11:11:11Z2015-12-18T11:11:11Z2015-12-1820152016-03-29T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePesquisa Agropecuária Brasileira, v. 50, n. 12, p. 1121-1130, dez. 2015.http://www.alice.cnptia.embrapa.br/alice/handle/doc/103199210.1590/S0100-204X2015001200001enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T03:26:40Zoai:www.alice.cnptia.embrapa.br:doc/1031992Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T03:26:40falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T03:26:40Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Characterization of rue extract and its potential for controlling rice blast.
title Characterization of rue extract and its potential for controlling rice blast.
spellingShingle Characterization of rue extract and its potential for controlling rice blast.
REIS, K. B.
Bergapteno
Psoraleno
Controle
Arroz
Arruda
Ruta graveolens
Brusone
Magnaporthe oryzae
title_short Characterization of rue extract and its potential for controlling rice blast.
title_full Characterization of rue extract and its potential for controlling rice blast.
title_fullStr Characterization of rue extract and its potential for controlling rice blast.
title_full_unstemmed Characterization of rue extract and its potential for controlling rice blast.
title_sort Characterization of rue extract and its potential for controlling rice blast.
author REIS, K. B.
author_facet REIS, K. B.
CORTÊS, M. V. de C. B.
MARTINS, F. S.
FILIPPI, M. C. C. de
PAULA, J. R. de
CONCEIÇÃO, E. C. da
author_role author
author2 CORTÊS, M. V. de C. B.
MARTINS, F. S.
FILIPPI, M. C. C. de
PAULA, J. R. de
CONCEIÇÃO, E. C. da
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv KARINNA BANNACH REIS, UFG; MARCIO VINICIUS DE C BARROS CORTES, CNPAF; FREDERICO SEVERINO MARTINS, USP, Ribeirão Preto-SP; MARTA CRISTINA CORSI DE FILIPPI, CNPAF; JOSE REALINO DE PAULA, UFG; EDEMILSON CARDOSO DA CONCEIÇÃO, UFG.
dc.contributor.author.fl_str_mv REIS, K. B.
CORTÊS, M. V. de C. B.
MARTINS, F. S.
FILIPPI, M. C. C. de
PAULA, J. R. de
CONCEIÇÃO, E. C. da
dc.subject.por.fl_str_mv Bergapteno
Psoraleno
Controle
Arroz
Arruda
Ruta graveolens
Brusone
Magnaporthe oryzae
topic Bergapteno
Psoraleno
Controle
Arroz
Arruda
Ruta graveolens
Brusone
Magnaporthe oryzae
description The objective of this work was to purify and standardize the extract of rue (Ruta graveolens) and evaluate its effect on Magnaporthe oryzae as an alternative to the integrated management of rice blast. The drug was characterized, the liquid extract was obtained, and the methodology for quantifying the standard markers furanocoumarins psoralen and bergapten was validated. Rue extract and the markers, solely or in combination, were assayed in vitro, as well as in greenhouse conditions, for their ability to suppress leaf blast, by the evaluation of mycelial growth, conidial germination, and appressorium formation. Rue extract inhibited M. oryzae mycelial growth (100%), conidial germination (LD50=0.237 mg), and the appressorium formation (LD50=0.121 mg); besides, the extract reduced leaf blast by 80.84%. Fluorescence microscopy showed that rue extract did not damage M. oryzae cell wall and plasma membrane, indicating another mode of action. Rue extract has a great potential for controlling rice leaf blast.
publishDate 2015
dc.date.none.fl_str_mv 2015-12-18T11:11:11Z
2015-12-18T11:11:11Z
2015-12-18
2015
2016-03-29T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Pesquisa Agropecuária Brasileira, v. 50, n. 12, p. 1121-1130, dez. 2015.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1031992
10.1590/S0100-204X2015001200001
identifier_str_mv Pesquisa Agropecuária Brasileira, v. 50, n. 12, p. 1121-1130, dez. 2015.
10.1590/S0100-204X2015001200001
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1031992
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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