Uroanalysis test paper as an alternative method to determine total glucosinolates in oilrapeseed

Detalhes bibliográficos
Autor(a) principal: Silveira, Expedito Paulo
Data de Publicação: 2014
Outros Autores: Gisler, Gastão C., Cerqueira, Marta Regina M. de
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/13487
Resumo: bench test was conducted, in 1985, using a crushed seed of each one of the twelve cultivars of Brassica napus L. vax. oleifera Metzg. and one of Brassica campestris L. available. After crusbing, the seeds were embibed on 1 ml of distilled water, during 24 hours at room temperature, and submitted to reaction on uroanalysis test paper containing glucose-oxidase-peroxidase and a chromogen for determining the concentration of glucose. The positive nonmesuared correlation between the results obtained through this glucose test and the data of determination of glucosinolates by gas/liquid chromatography for the same cultivars as published in the current bibliography, indicates the validity of that test for the mentioned purposes, although some methodological improvements are needed to acquire the desirable rapidity and accuracy.
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spelling Uroanalysis test paper as an alternative method to determine total glucosinolates in oilrapeseedTiras reagentes para uroanálise como método alternativo na determinação de glicosinolatos totais em sementes de colza.Brassica napus; Brassica campestrisBrassica napus; Brassica campestrisbench test was conducted, in 1985, using a crushed seed of each one of the twelve cultivars of Brassica napus L. vax. oleifera Metzg. and one of Brassica campestris L. available. After crusbing, the seeds were embibed on 1 ml of distilled water, during 24 hours at room temperature, and submitted to reaction on uroanalysis test paper containing glucose-oxidase-peroxidase and a chromogen for determining the concentration of glucose. The positive nonmesuared correlation between the results obtained through this glucose test and the data of determination of glucosinolates by gas/liquid chromatography for the same cultivars as published in the current bibliography, indicates the validity of that test for the mentioned purposes, although some methodological improvements are needed to acquire the desirable rapidity and accuracy.Foram desenvolvidos, em 1985, testes de laboratório em que se utilizou uma semente esmagada de cada uma das doze cultivares de Brassica napus L. var. oleifera Metzg. E de uma de Brassica campestris L. disponíveis. Após o esmagamento, as sementes foram embebidas em 0,1 ml de água destilada, durante 24 horas, à temperatura ambiente, e submetidas à reação com tira de papel para uroanálise, que contém glicose-oxidase-peroxidase e cromógeno, para determinar a concentração de glicose. A correlação positiva não-quantificada, existente entre os resultados obtidos no teste de glicose, e os dados de determinação de glicosinolatos totais por cromatografia fase líquida/gasosa, divulgados na literatura pertinente, para as mesmas cultivares, valida aquele teste para os propósitos mencionados, embora careça de aperfeiçoamentos metodológicos, para se adquirir a rapidez e exatidão desejáveis.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraSilveira, Expedito PauloGisler, Gastão C.Cerqueira, Marta Regina M. de2014-04-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/13487Pesquisa Agropecuaria Brasileira; v.25, n.5, maio 1990; 727-731Pesquisa Agropecuária Brasileira; v.25, n.5, maio 1990; 727-7311678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/13487/7619info:eu-repo/semantics/openAccess2014-04-17T14:34:29Zoai:ojs.seer.sct.embrapa.br:article/13487Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-04-17T14:34:29Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Uroanalysis test paper as an alternative method to determine total glucosinolates in oilrapeseed
Tiras reagentes para uroanálise como método alternativo na determinação de glicosinolatos totais em sementes de colza.
title Uroanalysis test paper as an alternative method to determine total glucosinolates in oilrapeseed
spellingShingle Uroanalysis test paper as an alternative method to determine total glucosinolates in oilrapeseed
Silveira, Expedito Paulo
Brassica napus; Brassica campestris
Brassica napus; Brassica campestris
title_short Uroanalysis test paper as an alternative method to determine total glucosinolates in oilrapeseed
title_full Uroanalysis test paper as an alternative method to determine total glucosinolates in oilrapeseed
title_fullStr Uroanalysis test paper as an alternative method to determine total glucosinolates in oilrapeseed
title_full_unstemmed Uroanalysis test paper as an alternative method to determine total glucosinolates in oilrapeseed
title_sort Uroanalysis test paper as an alternative method to determine total glucosinolates in oilrapeseed
author Silveira, Expedito Paulo
author_facet Silveira, Expedito Paulo
Gisler, Gastão C.
Cerqueira, Marta Regina M. de
author_role author
author2 Gisler, Gastão C.
Cerqueira, Marta Regina M. de
author2_role author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Silveira, Expedito Paulo
Gisler, Gastão C.
Cerqueira, Marta Regina M. de
dc.subject.por.fl_str_mv Brassica napus; Brassica campestris
Brassica napus; Brassica campestris
topic Brassica napus; Brassica campestris
Brassica napus; Brassica campestris
description bench test was conducted, in 1985, using a crushed seed of each one of the twelve cultivars of Brassica napus L. vax. oleifera Metzg. and one of Brassica campestris L. available. After crusbing, the seeds were embibed on 1 ml of distilled water, during 24 hours at room temperature, and submitted to reaction on uroanalysis test paper containing glucose-oxidase-peroxidase and a chromogen for determining the concentration of glucose. The positive nonmesuared correlation between the results obtained through this glucose test and the data of determination of glucosinolates by gas/liquid chromatography for the same cultivars as published in the current bibliography, indicates the validity of that test for the mentioned purposes, although some methodological improvements are needed to acquire the desirable rapidity and accuracy.
publishDate 2014
dc.date.none.fl_str_mv 2014-04-17
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/13487
url https://seer.sct.embrapa.br/index.php/pab/article/view/13487
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/13487/7619
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.25, n.5, maio 1990; 727-731
Pesquisa Agropecuária Brasileira; v.25, n.5, maio 1990; 727-731
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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