Identification of zygotic and nucellar seedlings in polyembryonic mango cultivars
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Agropecuária Brasileira (Online) |
Texto Completo: | https://seer.sct.embrapa.br/index.php/pab/article/view/12875 |
Resumo: | The objective of this work was to evaluate the occurrence of polyembryony in the mango cultivars Manila and Ataulfo, and to determine whether seedlings cultured in vitro are zygotic or nucelar. Percentage of polyembryony was calculated and the number of embryos in 100 seeds of each cultivar was recorded. 'Manila' exhibited 97% polyembryony with 3.4 embryos per seed, while 'Ataulfo' had 95% polyembryony with 3.2 embryos per seed. Later, 20 seeds of each cultivar were established in vitro, and it was analyzed those in which all embryos germinated (12 seeds from 'Manila' and 7 from 'Ataulfo'). DNA was extracted from seedling leaf tissue, and its origin was identified with 14 RAPD primers. The polymorphic markers recognized the seedlings of sexual origin in seven of nine 'Manila' polyembryonic seeds, and in four of seven 'Ataulfo' ones. Also, in polyembryonic seeds not all zygotic seedlings were produced by small embryos located at the micropyle. |
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Identification of zygotic and nucellar seedlings in polyembryonic mango cultivarsIdentificação de plântulas zigóticas e nucelares em cultivares de manga poliembriônicasMangifera indica; embryo culture; funiculus; molecular markers; polymorphismMangifera indica; cultura de embriões; funículo; marcadores moleculares; polimorfismoThe objective of this work was to evaluate the occurrence of polyembryony in the mango cultivars Manila and Ataulfo, and to determine whether seedlings cultured in vitro are zygotic or nucelar. Percentage of polyembryony was calculated and the number of embryos in 100 seeds of each cultivar was recorded. 'Manila' exhibited 97% polyembryony with 3.4 embryos per seed, while 'Ataulfo' had 95% polyembryony with 3.2 embryos per seed. Later, 20 seeds of each cultivar were established in vitro, and it was analyzed those in which all embryos germinated (12 seeds from 'Manila' and 7 from 'Ataulfo'). DNA was extracted from seedling leaf tissue, and its origin was identified with 14 RAPD primers. The polymorphic markers recognized the seedlings of sexual origin in seven of nine 'Manila' polyembryonic seeds, and in four of seven 'Ataulfo' ones. Also, in polyembryonic seeds not all zygotic seedlings were produced by small embryos located at the micropyle. O objetivo deste trabalho foi avaliar a ocorrência de poliembrionia nas mangas 'Manila' e 'Ataulfo' e determinar se as plântulas cultivadas in vitro são zigóticas ou nucelares. A percentagem de poliembrionia foi calculada e o número de embriões em 100 sementes de cada cultivar foi determinado. 'Manila' apresentou 97% de poliembrionia com 3,4 embriões por semente, enquanto 'Ataulfo' teve 95% de poliembrionia com 3,2 embriões por semente. Posteriormente, 20 sementes de cada cultivar foram cultivadas in vitro, tendose analisado aquelas em que todos os embriões germinaram (12 sementes de 'Manila' e sete de 'Ataulfo'). O DNA foi extraído a partir de tecidos foliares das plântulas, e sua origem foi identificada com 14 iniciadores RAPD. Os marcadores polimórficos reconheceram o embrião de origem sexual em sete das nove sementes poliembriônicas de 'Manila' e em quatro das sete sementes de 'Ataulfo'. Além disso, nem todas as plântulas zigóticas foram produzidas por embriões pequenos localizados no micrófilo das sementes poliembriônicas. Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraSAGARPA-CONACYT (Project num. 48595), Colegio de Postgraduados e Universidad Autónoma del Estado de MorelosMartinez Ochoa, Elisa del CarmenAndrade-Rodríguez, MariaRocandio Rodríguez, MarioVillegas Monter, Angel2012-12-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/12875Pesquisa Agropecuaria Brasileira; v.47, n.11, nov. 2012; 1629-1636Pesquisa Agropecuária Brasileira; v.47, n.11, nov. 2012; 1629-16361678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/12875/8229https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/12875/8592https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/12875/8594https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/12875/8595info:eu-repo/semantics/openAccess2012-12-19T18:13:57Zoai:ojs.seer.sct.embrapa.br:article/12875Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2012-12-19T18:13:57Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Identification of zygotic and nucellar seedlings in polyembryonic mango cultivars Identificação de plântulas zigóticas e nucelares em cultivares de manga poliembriônicas |
title |
Identification of zygotic and nucellar seedlings in polyembryonic mango cultivars |
spellingShingle |
Identification of zygotic and nucellar seedlings in polyembryonic mango cultivars Martinez Ochoa, Elisa del Carmen Mangifera indica; embryo culture; funiculus; molecular markers; polymorphism Mangifera indica; cultura de embriões; funículo; marcadores moleculares; polimorfismo |
title_short |
Identification of zygotic and nucellar seedlings in polyembryonic mango cultivars |
title_full |
Identification of zygotic and nucellar seedlings in polyembryonic mango cultivars |
title_fullStr |
Identification of zygotic and nucellar seedlings in polyembryonic mango cultivars |
title_full_unstemmed |
Identification of zygotic and nucellar seedlings in polyembryonic mango cultivars |
title_sort |
Identification of zygotic and nucellar seedlings in polyembryonic mango cultivars |
author |
Martinez Ochoa, Elisa del Carmen |
author_facet |
Martinez Ochoa, Elisa del Carmen Andrade-Rodríguez, Maria Rocandio Rodríguez, Mario Villegas Monter, Angel |
author_role |
author |
author2 |
Andrade-Rodríguez, Maria Rocandio Rodríguez, Mario Villegas Monter, Angel |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
SAGARPA-CONACYT (Project num. 48595), Colegio de Postgraduados e Universidad Autónoma del Estado de Morelos |
dc.contributor.author.fl_str_mv |
Martinez Ochoa, Elisa del Carmen Andrade-Rodríguez, Maria Rocandio Rodríguez, Mario Villegas Monter, Angel |
dc.subject.por.fl_str_mv |
Mangifera indica; embryo culture; funiculus; molecular markers; polymorphism Mangifera indica; cultura de embriões; funículo; marcadores moleculares; polimorfismo |
topic |
Mangifera indica; embryo culture; funiculus; molecular markers; polymorphism Mangifera indica; cultura de embriões; funículo; marcadores moleculares; polimorfismo |
description |
The objective of this work was to evaluate the occurrence of polyembryony in the mango cultivars Manila and Ataulfo, and to determine whether seedlings cultured in vitro are zygotic or nucelar. Percentage of polyembryony was calculated and the number of embryos in 100 seeds of each cultivar was recorded. 'Manila' exhibited 97% polyembryony with 3.4 embryos per seed, while 'Ataulfo' had 95% polyembryony with 3.2 embryos per seed. Later, 20 seeds of each cultivar were established in vitro, and it was analyzed those in which all embryos germinated (12 seeds from 'Manila' and 7 from 'Ataulfo'). DNA was extracted from seedling leaf tissue, and its origin was identified with 14 RAPD primers. The polymorphic markers recognized the seedlings of sexual origin in seven of nine 'Manila' polyembryonic seeds, and in four of seven 'Ataulfo' ones. Also, in polyembryonic seeds not all zygotic seedlings were produced by small embryos located at the micropyle. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-12-17 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/12875 |
url |
https://seer.sct.embrapa.br/index.php/pab/article/view/12875 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/12875/8229 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/12875/8592 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/12875/8594 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/12875/8595 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
dc.source.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira; v.47, n.11, nov. 2012; 1629-1636 Pesquisa Agropecuária Brasileira; v.47, n.11, nov. 2012; 1629-1636 1678-3921 0100-104x reponame:Pesquisa Agropecuária Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Agropecuária Brasileira (Online) |
collection |
Pesquisa Agropecuária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
pab@sct.embrapa.br || sct.pab@embrapa.br |
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1793416673166884864 |