Somatic embryogenesis and the effect of particle bombardment on banana Maçã regeneration
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Agropecuária Brasileira (Online) |
Texto Completo: | https://seer.sct.embrapa.br/index.php/pab/article/view/7068 |
Resumo: | A plant regeneration method with cell suspension cultures of banana, and the effect of biobalistic on regeneration potential are described in this report. Somatic embryos of banana were obtained from indirect embryogenesis of male inflorescence of banana cultivar Maçã (AAB group). Part of the calluses formed (40%) showed embryogenic characteristics (nonfriable, compact and yellow color). The cell suspension, originated from embryogenic calluses, contained clusters of small tightly packed cells with dense cytoplasms, relatively large nuclei and very dense nucleoli. After four months of culture, somatic embryos started to regenerate. The maximum number of regenerated plants was observed between 45 and 60 days after embryo formation. In the first experiment, 401 plants were regenerated from approximately 10 mL of packed cells. In the second experiment, 399 plants were regenerated from a cell suspension six months older than that of the first experiment. Cell transformation using particle bombardment with three different plasmid constructions, containing the uid-A gene, resulted in a strong GUS expression five days after bombardment; however, plant regeneration from bombarded cells was much lower than nonbombarded ones. |
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Somatic embryogenesis and the effect of particle bombardment on banana Maçã regenerationEmbriogênese somática e o efeito do bombardeamento de partículas na regeneração de banana cv. MaçãMusa; cell suspension; callus; somatic differentiation; biobalisticMusa; suspensão celular; calos; diferenciação somática; biobalística A plant regeneration method with cell suspension cultures of banana, and the effect of biobalistic on regeneration potential are described in this report. Somatic embryos of banana were obtained from indirect embryogenesis of male inflorescence of banana cultivar Maçã (AAB group). Part of the calluses formed (40%) showed embryogenic characteristics (nonfriable, compact and yellow color). The cell suspension, originated from embryogenic calluses, contained clusters of small tightly packed cells with dense cytoplasms, relatively large nuclei and very dense nucleoli. After four months of culture, somatic embryos started to regenerate. The maximum number of regenerated plants was observed between 45 and 60 days after embryo formation. In the first experiment, 401 plants were regenerated from approximately 10 mL of packed cells. In the second experiment, 399 plants were regenerated from a cell suspension six months older than that of the first experiment. Cell transformation using particle bombardment with three different plasmid constructions, containing the uid-A gene, resulted in a strong GUS expression five days after bombardment; however, plant regeneration from bombarded cells was much lower than nonbombarded ones.Neste trabalho é descrito um método de regeneração de plantas, a partir de células de bananeira, em suspensão, e o efeito da biobalística no potencial regenerativo. Embriões somáticos foram obtidos de inflorescências masculinas de bananeira cv. Maçã (grupo AAB) por meio de embriogênese indireta. Parte dos calos (40%) apresentou características embriogênicas (não-friáveis, compactas e amareladas). Suspensões celulares obtidas desses calos continham pequenas massas celulares, com citoplasmas ricos em grânulos de amido, núcleos grandes e nucléolos densos. Após quatro meses, embriões somáticos começaram a se desenvolver. O número máximo de plantas regeneradas ocorreu 45–60 dias após a formação dos embriões. No primeiro experimento foram regeneradas 401 plantas. No segundo, 399 plantas foram obtidas de uma suspensão celular 6 meses mais velha do que a do primeiro experimento. Transformações celulares com uma das três construções plasmidiais utilizadas, que continham o gene uid-A, resultaram em fortes sinais de expressão cinco dias após as transformações; todavia, o número de plantas regeneradas foi muito menor do que o observado no material não bombardeado.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraHoullou-Kido, Laureen MichelleKido, Ederson AkioFalco, Maria CristinaSilva Filho, Márcio de CastroFigueira, Antônio Vargas de OliveiraNogueira, Neusa de LimaRossi, Mônica LanzoniTulmann Neto, Augusto2005-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/7068Pesquisa Agropecuaria Brasileira; v.40, n.11, nov. 2005; 1081-1086Pesquisa Agropecuária Brasileira; v.40, n.11, nov. 2005; 1081-10861678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/7068/4116info:eu-repo/semantics/openAccess2014-09-19T17:37:38Zoai:ojs.seer.sct.embrapa.br:article/7068Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-09-19T17:37:38Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Somatic embryogenesis and the effect of particle bombardment on banana Maçã regeneration Embriogênese somática e o efeito do bombardeamento de partículas na regeneração de banana cv. Maçã |
title |
Somatic embryogenesis and the effect of particle bombardment on banana Maçã regeneration |
spellingShingle |
Somatic embryogenesis and the effect of particle bombardment on banana Maçã regeneration Houllou-Kido, Laureen Michelle Musa; cell suspension; callus; somatic differentiation; biobalistic Musa; suspensão celular; calos; diferenciação somática; biobalística |
title_short |
Somatic embryogenesis and the effect of particle bombardment on banana Maçã regeneration |
title_full |
Somatic embryogenesis and the effect of particle bombardment on banana Maçã regeneration |
title_fullStr |
Somatic embryogenesis and the effect of particle bombardment on banana Maçã regeneration |
title_full_unstemmed |
Somatic embryogenesis and the effect of particle bombardment on banana Maçã regeneration |
title_sort |
Somatic embryogenesis and the effect of particle bombardment on banana Maçã regeneration |
author |
Houllou-Kido, Laureen Michelle |
author_facet |
Houllou-Kido, Laureen Michelle Kido, Ederson Akio Falco, Maria Cristina Silva Filho, Márcio de Castro Figueira, Antônio Vargas de Oliveira Nogueira, Neusa de Lima Rossi, Mônica Lanzoni Tulmann Neto, Augusto |
author_role |
author |
author2 |
Kido, Ederson Akio Falco, Maria Cristina Silva Filho, Márcio de Castro Figueira, Antônio Vargas de Oliveira Nogueira, Neusa de Lima Rossi, Mônica Lanzoni Tulmann Neto, Augusto |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
|
dc.contributor.author.fl_str_mv |
Houllou-Kido, Laureen Michelle Kido, Ederson Akio Falco, Maria Cristina Silva Filho, Márcio de Castro Figueira, Antônio Vargas de Oliveira Nogueira, Neusa de Lima Rossi, Mônica Lanzoni Tulmann Neto, Augusto |
dc.subject.por.fl_str_mv |
Musa; cell suspension; callus; somatic differentiation; biobalistic Musa; suspensão celular; calos; diferenciação somática; biobalística |
topic |
Musa; cell suspension; callus; somatic differentiation; biobalistic Musa; suspensão celular; calos; diferenciação somática; biobalística |
description |
A plant regeneration method with cell suspension cultures of banana, and the effect of biobalistic on regeneration potential are described in this report. Somatic embryos of banana were obtained from indirect embryogenesis of male inflorescence of banana cultivar Maçã (AAB group). Part of the calluses formed (40%) showed embryogenic characteristics (nonfriable, compact and yellow color). The cell suspension, originated from embryogenic calluses, contained clusters of small tightly packed cells with dense cytoplasms, relatively large nuclei and very dense nucleoli. After four months of culture, somatic embryos started to regenerate. The maximum number of regenerated plants was observed between 45 and 60 days after embryo formation. In the first experiment, 401 plants were regenerated from approximately 10 mL of packed cells. In the second experiment, 399 plants were regenerated from a cell suspension six months older than that of the first experiment. Cell transformation using particle bombardment with three different plasmid constructions, containing the uid-A gene, resulted in a strong GUS expression five days after bombardment; however, plant regeneration from bombarded cells was much lower than nonbombarded ones. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-11-01 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/7068 |
url |
https://seer.sct.embrapa.br/index.php/pab/article/view/7068 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/7068/4116 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
dc.source.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira; v.40, n.11, nov. 2005; 1081-1086 Pesquisa Agropecuária Brasileira; v.40, n.11, nov. 2005; 1081-1086 1678-3921 0100-104x reponame:Pesquisa Agropecuária Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Agropecuária Brasileira (Online) |
collection |
Pesquisa Agropecuária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
pab@sct.embrapa.br || sct.pab@embrapa.br |
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1793416698884259840 |