Somatic embryogenesis of an early cotton cultivar

Detalhes bibliográficos
Autor(a) principal: González-Benito, María Elena
Data de Publicação: 1997
Outros Autores: Frota-Chagas Carvalho, Julita María, Pérez, César
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/4680
Resumo: Somatic embryogenesis in cotton has been previously reported in a limited number of genotypes, predominantly Coker cultivars. Before tissue culture techniques are widely applied to cotton improvement programs, regeneration must be possible for a broad range of genotypes. The aim of the present work was to develop a method to obtain somatic embryoids of an early cotton cultivar (Gossypium hirsutum L. race latifolium Hutch cv. CNPA Precoce 2) and their subsequent development. Callus induction was attempted with cotyledon and hypocotyl explants. These explants were cultured on MS medium supplemented with five concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and N6-(2-isopentenyl)-adenine (2iP) either alone or in combination. Based on callus appearance (light brown and granular), four different growth regulator combinations were selected for further callus development. Callus was subcultured on 2.45 μM 2iP and subsequently transferred to 0.45 and 22.50 μM 2,4-D. Somatic embryos of different sizes and shapes subsequently appeared on MS medium supplemented with 2 g L-1 glutamine and no growth regulators. Plantlets were developed from those embryoids. Plant regeneration through somatic embryogenesis is achieved for the first time in the early cultivar CNPA Precoce 2.
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spelling Somatic embryogenesis of an early cotton cultivarEmbriogênese somática na cultivar precoce do algodãocallus; somatic embryos; plant regenerationcalos; embriões somáticos; regeneração da plantaSomatic embryogenesis in cotton has been previously reported in a limited number of genotypes, predominantly Coker cultivars. Before tissue culture techniques are widely applied to cotton improvement programs, regeneration must be possible for a broad range of genotypes. The aim of the present work was to develop a method to obtain somatic embryoids of an early cotton cultivar (Gossypium hirsutum L. race latifolium Hutch cv. CNPA Precoce 2) and their subsequent development. Callus induction was attempted with cotyledon and hypocotyl explants. These explants were cultured on MS medium supplemented with five concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and N6-(2-isopentenyl)-adenine (2iP) either alone or in combination. Based on callus appearance (light brown and granular), four different growth regulator combinations were selected for further callus development. Callus was subcultured on 2.45 μM 2iP and subsequently transferred to 0.45 and 22.50 μM 2,4-D. Somatic embryos of different sizes and shapes subsequently appeared on MS medium supplemented with 2 g L-1 glutamine and no growth regulators. Plantlets were developed from those embryoids. Plant regeneration through somatic embryogenesis is achieved for the first time in the early cultivar CNPA Precoce 2.A embriogênese somática no algodão tem sido observada num número limitado de genótipos, predominantemente nas cultivares Coker. Como as técnicas de cultura de tecido são freqüentemente utilizadas nos programas de melhoramento do algodão, a regeneração das plântulas deve ser possível num grande número de genótipos. A finalidade do presente trabalho foi desenvolver um método para obtenção de embriões somáticos na cultivar precoce de algodão (Gossypium hirsutum L. raça latifolium Hutch cv. CNPA Precoce 2) e seu subseqüente desenvolvimento. Estudou-se a indução de calo nos explantes de cotilédone e de hipocótilo. Estes explantes foram cultivados em meio MS suplementado com cinco concentrações de ácido 2,4-diclorofenoxiacético (2,4-D) e N6-Δ2-isopentenil adenina (2iP) isolado ou em combinação. Com base na aparência dos calos (bege e granulado), foram selecionadas quatro diferentes combinações dos reguladores de crescimento para o melhor desenvolvimento dos calos. Os calos foram induzidos em 2,45 μM de 2iP, e, posteriormente transferidos para 0,45 e 22,50 μM 2,4-D. Embriões somáticos de diferentes formas e tamanhos aparecem subseqüentemente em meio MS desprovido de reguladores de crescimento e com 2 g L-1 glutamina. Várias plântulas se desenvolveram a partir destes embriões somáticos. A regeneração da planta por meio da embriogênese somática foi conseguida, pela primeira vez, na cultivar CNPA Precoce 2.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraGonzález-Benito, María ElenaFrota-Chagas Carvalho, Julita MaríaPérez, César1997-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/4680Pesquisa Agropecuaria Brasileira; v.32, n.5, maio 1997; 485-488Pesquisa Agropecuária Brasileira; v.32, n.5, maio 1997; 485-4881678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/4680/7360info:eu-repo/semantics/openAccess2015-03-19T17:54:22Zoai:ojs.seer.sct.embrapa.br:article/4680Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2015-03-19T17:54:22Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Somatic embryogenesis of an early cotton cultivar
Embriogênese somática na cultivar precoce do algodão
title Somatic embryogenesis of an early cotton cultivar
spellingShingle Somatic embryogenesis of an early cotton cultivar
González-Benito, María Elena
callus; somatic embryos; plant regeneration
calos; embriões somáticos; regeneração da planta
title_short Somatic embryogenesis of an early cotton cultivar
title_full Somatic embryogenesis of an early cotton cultivar
title_fullStr Somatic embryogenesis of an early cotton cultivar
title_full_unstemmed Somatic embryogenesis of an early cotton cultivar
title_sort Somatic embryogenesis of an early cotton cultivar
author González-Benito, María Elena
author_facet González-Benito, María Elena
Frota-Chagas Carvalho, Julita María
Pérez, César
author_role author
author2 Frota-Chagas Carvalho, Julita María
Pérez, César
author2_role author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv González-Benito, María Elena
Frota-Chagas Carvalho, Julita María
Pérez, César
dc.subject.por.fl_str_mv callus; somatic embryos; plant regeneration
calos; embriões somáticos; regeneração da planta
topic callus; somatic embryos; plant regeneration
calos; embriões somáticos; regeneração da planta
description Somatic embryogenesis in cotton has been previously reported in a limited number of genotypes, predominantly Coker cultivars. Before tissue culture techniques are widely applied to cotton improvement programs, regeneration must be possible for a broad range of genotypes. The aim of the present work was to develop a method to obtain somatic embryoids of an early cotton cultivar (Gossypium hirsutum L. race latifolium Hutch cv. CNPA Precoce 2) and their subsequent development. Callus induction was attempted with cotyledon and hypocotyl explants. These explants were cultured on MS medium supplemented with five concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and N6-(2-isopentenyl)-adenine (2iP) either alone or in combination. Based on callus appearance (light brown and granular), four different growth regulator combinations were selected for further callus development. Callus was subcultured on 2.45 μM 2iP and subsequently transferred to 0.45 and 22.50 μM 2,4-D. Somatic embryos of different sizes and shapes subsequently appeared on MS medium supplemented with 2 g L-1 glutamine and no growth regulators. Plantlets were developed from those embryoids. Plant regeneration through somatic embryogenesis is achieved for the first time in the early cultivar CNPA Precoce 2.
publishDate 1997
dc.date.none.fl_str_mv 1997-05-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/4680
url https://seer.sct.embrapa.br/index.php/pab/article/view/4680
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/4680/7360
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.32, n.5, maio 1997; 485-488
Pesquisa Agropecuária Brasileira; v.32, n.5, maio 1997; 485-488
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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