Outcrossing rate between 'Haden' and 'Tommy Atkins' mangoes estimated using microsatellite and AFLP markers

Detalhes bibliográficos
Autor(a) principal: Santos, Carlos Antonio Fernandes
Data de Publicação: 2011
Outros Autores: Lima Neto, Francisco Pinheiro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/10700
Resumo: The objective of this work was to estimate outcrossing rates between Haden and Tommy Atkins mango cultivars, using AFLP and microsatellite markers. Progenies of an isolated 'Haden' plant, identified in a 'Tommy Atkins' commercial orchard, in Petrolina, PE, Brazil, were analyzed. Total DNA was isolated from the progeny leaves and used for AFLP and microsatellite reactions. Multilocus outcrossing rates (tm) were estimated by direct count of AFLP or microsatellite markers and by the mLTR software. Outcrossing rates ranged from 0.85 to 0.87 with the analysis based on seven AFLP markers, and from 0.83 to 0.91 based on three microsatellite primers. No unexpected band patterns were observed for 'Haden' and 'Tommy Atkins'. The estimates obtained with the mLTR software were close to those obtained by direct AFLP and microsatellite allele counting, which indicates that the multilocus model was appropriate for this kind of study. The microsatellites mMiCIR005, mMiCIR030, and mMiCIR036 can be used to elucidate the origin of 'Haden' and 'Tommy Atkins' seedlings.
id EMBRAPA-4_d2c8ea5b7126b80bf6b81ad01a524a56
oai_identifier_str oai:ojs.seer.sct.embrapa.br:article/10700
network_acronym_str EMBRAPA-4
network_name_str Pesquisa Agropecuária Brasileira (Online)
repository_id_str
spelling Outcrossing rate between 'Haden' and 'Tommy Atkins' mangoes estimated using microsatellite and AFLP markersTaxa de cruzamento entre manga 'Haden' e 'Tommy Atkins' estimada com marcadores microssatélites e AFLPMangifera indica; mLTR; multilocus estimation; SSRMangifera indica; mLTR; estimativa multilocos; SSRThe objective of this work was to estimate outcrossing rates between Haden and Tommy Atkins mango cultivars, using AFLP and microsatellite markers. Progenies of an isolated 'Haden' plant, identified in a 'Tommy Atkins' commercial orchard, in Petrolina, PE, Brazil, were analyzed. Total DNA was isolated from the progeny leaves and used for AFLP and microsatellite reactions. Multilocus outcrossing rates (tm) were estimated by direct count of AFLP or microsatellite markers and by the mLTR software. Outcrossing rates ranged from 0.85 to 0.87 with the analysis based on seven AFLP markers, and from 0.83 to 0.91 based on three microsatellite primers. No unexpected band patterns were observed for 'Haden' and 'Tommy Atkins'. The estimates obtained with the mLTR software were close to those obtained by direct AFLP and microsatellite allele counting, which indicates that the multilocus model was appropriate for this kind of study. The microsatellites mMiCIR005, mMiCIR030, and mMiCIR036 can be used to elucidate the origin of 'Haden' and 'Tommy Atkins' seedlings. O objetivo deste trabalho foi estimar a taxa de polinização cruzada entre as cultivares de manga Haden e Tommy Atkins, por meio do uso de marcadores AFLP e microssatélites. Progênies de uma planta isolada de 'Haden', identificada em plantio comercial de 'Tommy Atkins', em Petrolina, PE, foram analisadas. O DNA total foi isolado das folhas das progênies e utilizado em reações com marcadores AFLP e microssatélites. Estimativas multilocos (tm) foram obtidas pela contagem direta de marcas AFLP ou de microssatélites e pelo programa mLTR. A taxa de polinização cruzada variou de 0,85 a 0,87, com análise baseada em sete marcas AFLP, e de 0,83 a 0,91, com base em três microssatélites. Não foram observadas bandas diferentes das esperadas para 'Haden' e 'Tommy Atkins'. As estimativas obtidas com o programa mLTR foram próximas às obtidas com a contagem direta de marcas AFLP e de microssatélites, o que indica que o modelo multilocos foi apropriado para este tipo de trabalho. Os microssatélites mMiCIR005, mMiCIR030 e mMiCIR036 são recomendados para elucidar a origem de mudas de 'Haden' e 'Tommy Atkins'.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraSantos, Carlos Antonio FernandesLima Neto, Francisco Pinheiro2011-10-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/10700Pesquisa Agropecuaria Brasileira; v.46, n.8, ago. 2011; 899-904Pesquisa Agropecuária Brasileira; v.46, n.8, ago. 2011; 899-9041678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/10700/6497https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/10700/6091info:eu-repo/semantics/openAccess2014-06-05T17:47:27Zoai:ojs.seer.sct.embrapa.br:article/10700Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-06-05T17:47:27Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Outcrossing rate between 'Haden' and 'Tommy Atkins' mangoes estimated using microsatellite and AFLP markers
Taxa de cruzamento entre manga 'Haden' e 'Tommy Atkins' estimada com marcadores microssatélites e AFLP
title Outcrossing rate between 'Haden' and 'Tommy Atkins' mangoes estimated using microsatellite and AFLP markers
spellingShingle Outcrossing rate between 'Haden' and 'Tommy Atkins' mangoes estimated using microsatellite and AFLP markers
Santos, Carlos Antonio Fernandes
Mangifera indica; mLTR; multilocus estimation; SSR
Mangifera indica; mLTR; estimativa multilocos; SSR
title_short Outcrossing rate between 'Haden' and 'Tommy Atkins' mangoes estimated using microsatellite and AFLP markers
title_full Outcrossing rate between 'Haden' and 'Tommy Atkins' mangoes estimated using microsatellite and AFLP markers
title_fullStr Outcrossing rate between 'Haden' and 'Tommy Atkins' mangoes estimated using microsatellite and AFLP markers
title_full_unstemmed Outcrossing rate between 'Haden' and 'Tommy Atkins' mangoes estimated using microsatellite and AFLP markers
title_sort Outcrossing rate between 'Haden' and 'Tommy Atkins' mangoes estimated using microsatellite and AFLP markers
author Santos, Carlos Antonio Fernandes
author_facet Santos, Carlos Antonio Fernandes
Lima Neto, Francisco Pinheiro
author_role author
author2 Lima Neto, Francisco Pinheiro
author2_role author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Santos, Carlos Antonio Fernandes
Lima Neto, Francisco Pinheiro
dc.subject.por.fl_str_mv Mangifera indica; mLTR; multilocus estimation; SSR
Mangifera indica; mLTR; estimativa multilocos; SSR
topic Mangifera indica; mLTR; multilocus estimation; SSR
Mangifera indica; mLTR; estimativa multilocos; SSR
description The objective of this work was to estimate outcrossing rates between Haden and Tommy Atkins mango cultivars, using AFLP and microsatellite markers. Progenies of an isolated 'Haden' plant, identified in a 'Tommy Atkins' commercial orchard, in Petrolina, PE, Brazil, were analyzed. Total DNA was isolated from the progeny leaves and used for AFLP and microsatellite reactions. Multilocus outcrossing rates (tm) were estimated by direct count of AFLP or microsatellite markers and by the mLTR software. Outcrossing rates ranged from 0.85 to 0.87 with the analysis based on seven AFLP markers, and from 0.83 to 0.91 based on three microsatellite primers. No unexpected band patterns were observed for 'Haden' and 'Tommy Atkins'. The estimates obtained with the mLTR software were close to those obtained by direct AFLP and microsatellite allele counting, which indicates that the multilocus model was appropriate for this kind of study. The microsatellites mMiCIR005, mMiCIR030, and mMiCIR036 can be used to elucidate the origin of 'Haden' and 'Tommy Atkins' seedlings.
publishDate 2011
dc.date.none.fl_str_mv 2011-10-26
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/10700
url https://seer.sct.embrapa.br/index.php/pab/article/view/10700
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/10700/6497
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/10700/6091
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.46, n.8, ago. 2011; 899-904
Pesquisa Agropecuária Brasileira; v.46, n.8, ago. 2011; 899-904
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
_version_ 1793416677633818624

Registros relacionados