Functional diversity of bacterial genes associated with aromatic hydrocarbon degradation in anthropogenic dark earth of Amazonia

Detalhes bibliográficos
Autor(a) principal: Germano, Mariana Gomes
Data de Publicação: 2012
Outros Autores: Cannavan, Fabiana de Souza, Mendes, Lucas William, Lima, Amanda Barbosa, Teixeira, Wenceslau Geraldes, Pellizari, Vivian Helena, Tsai, Siu Mui
Tipo de documento: Artigo
Idioma: eng
por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/10028
Resumo: The objective of this work was to evaluate the catabolic gene diversity for the bacterial degradation of aromatic hydrocarbons in anthropogenic dark earth of Amazonia (ADE) and their biochar (BC). Functional diversity analyses in ADE soils can provide information on how adaptive microorganisms may influence the fertility of soils and what is their involvement in biogeochemical cycles. For this, clone libraries containing the gene encoding for the alpha subunit of aromatic ring‑hydroxylating dioxygenases (α‑ARHD bacterial gene) were constructed, totaling 800 clones. These libraries were prepared from samples of an ADE soil under two different land uses, located at the Caldeirão Experimental Station – secondary forest (SF) and agriculture (AG) –, and the biochar (SF_BC and AG_BC, respectively). Heterogeneity estimates indicated greater diversity in BC libraries; and Venn diagrams showed more unique operational protein clusters (OPC) in the SF_BC library than the ADE soil, which indicates that specific metabolic processes may occur in biochar. Phylogenetic analysis showed unidentified dioxygenases in ADE soils. Libraries containing functional gene encoding for the alpha subunit of the aromatic ring‑hydroxylating dioxygenases (ARHD) gene from biochar show higher diversity indices than those of ADE under secondary forest and agriculture.
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spelling Functional diversity of bacterial genes associated with aromatic hydrocarbon degradation in anthropogenic dark earth of AmazoniaFunctional diversity of bacterial genes associated with aromatic hydrocarbon degradation in anthropogenic dark earth of Amazoniagenes catabólicos; diversidade genética; sistemas de uso da terra; ecologia microbiana; solos tropicaiscatabolic genes; genetic diversity; land use systems; microbial ecology; tropical soilsThe objective of this work was to evaluate the catabolic gene diversity for the bacterial degradation of aromatic hydrocarbons in anthropogenic dark earth of Amazonia (ADE) and their biochar (BC). Functional diversity analyses in ADE soils can provide information on how adaptive microorganisms may influence the fertility of soils and what is their involvement in biogeochemical cycles. For this, clone libraries containing the gene encoding for the alpha subunit of aromatic ring‑hydroxylating dioxygenases (α‑ARHD bacterial gene) were constructed, totaling 800 clones. These libraries were prepared from samples of an ADE soil under two different land uses, located at the Caldeirão Experimental Station – secondary forest (SF) and agriculture (AG) –, and the biochar (SF_BC and AG_BC, respectively). Heterogeneity estimates indicated greater diversity in BC libraries; and Venn diagrams showed more unique operational protein clusters (OPC) in the SF_BC library than the ADE soil, which indicates that specific metabolic processes may occur in biochar. Phylogenetic analysis showed unidentified dioxygenases in ADE soils. Libraries containing functional gene encoding for the alpha subunit of the aromatic ring‑hydroxylating dioxygenases (ARHD) gene from biochar show higher diversity indices than those of ADE under secondary forest and agriculture.The objective of this work was to evaluate the catabolic gene diversity for the bacterial degradation of aromatic hydrocarbons in anthropogenic dark earth of Amazonia (ADE) and their biochar (BC). Functional diversity analyses in ADE soils can provide information on how adaptive microorganisms may influence the fertility of soils and what is their involvement in biogeochemical cycles. For this, clone libraries containing the gene encoding for the alpha subunit of aromatic ring‑hydroxylating dioxygenases (α‑ARHD bacterial gene) were constructed, totaling 800 clones. These libraries were prepared from samples of an ADE soil under two different land uses, located at the Caldeirão Experimental Station – secondary forest (SF) and agriculture (AG) –, and the biochar (SF_BC and AG_BC, respectively). Heterogeneity estimates indicated greater diversity in BC libraries; and Venn diagrams showed more unique operational protein clusters (OPC) in the SF_BC library than the ADE soil, which indicates that specific metabolic processes may occur in biochar. Phylogenetic analysis showed unidentified dioxygenases in ADE soils. Libraries containing functional gene encoding for the alpha subunit of the aromatic ring‑hydroxylating dioxygenases (ARHD) gene from biochar show higher diversity indices than those of ADE under secondary forest and agriculture.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraFapespCNPqGermano, Mariana GomesCannavan, Fabiana de SouzaMendes, Lucas WilliamLima, Amanda BarbosaTeixeira, Wenceslau GeraldesPellizari, Vivian HelenaTsai, Siu Mui2012-06-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/10028Pesquisa Agropecuaria Brasileira; v.47, n.5, maio 2012: 3rd International Biochar Conference; 654-664Pesquisa Agropecuária Brasileira; v.47, n.5, maio 2012: 3rd International Biochar Conference; 654-6641678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAengporhttps://seer.sct.embrapa.br/index.php/pab/article/view/10028/6912https://seer.sct.embrapa.br/index.php/pab/article/view/10028/7428https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/10028/5349https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/10028/8045info:eu-repo/semantics/openAccess2012-08-02T14:33:16Zoai:ojs.seer.sct.embrapa.br:article/10028Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2012-08-02T14:33:16Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Functional diversity of bacterial genes associated with aromatic hydrocarbon degradation in anthropogenic dark earth of Amazonia
Functional diversity of bacterial genes associated with aromatic hydrocarbon degradation in anthropogenic dark earth of Amazonia
title Functional diversity of bacterial genes associated with aromatic hydrocarbon degradation in anthropogenic dark earth of Amazonia
spellingShingle Functional diversity of bacterial genes associated with aromatic hydrocarbon degradation in anthropogenic dark earth of Amazonia
Germano, Mariana Gomes
genes catabólicos; diversidade genética; sistemas de uso da terra; ecologia microbiana; solos tropicais
catabolic genes; genetic diversity; land use systems; microbial ecology; tropical soils
title_short Functional diversity of bacterial genes associated with aromatic hydrocarbon degradation in anthropogenic dark earth of Amazonia
title_full Functional diversity of bacterial genes associated with aromatic hydrocarbon degradation in anthropogenic dark earth of Amazonia
title_fullStr Functional diversity of bacterial genes associated with aromatic hydrocarbon degradation in anthropogenic dark earth of Amazonia
title_full_unstemmed Functional diversity of bacterial genes associated with aromatic hydrocarbon degradation in anthropogenic dark earth of Amazonia
title_sort Functional diversity of bacterial genes associated with aromatic hydrocarbon degradation in anthropogenic dark earth of Amazonia
author Germano, Mariana Gomes
author_facet Germano, Mariana Gomes
Cannavan, Fabiana de Souza
Mendes, Lucas William
Lima, Amanda Barbosa
Teixeira, Wenceslau Geraldes
Pellizari, Vivian Helena
Tsai, Siu Mui
author_role author
author2 Cannavan, Fabiana de Souza
Mendes, Lucas William
Lima, Amanda Barbosa
Teixeira, Wenceslau Geraldes
Pellizari, Vivian Helena
Tsai, Siu Mui
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Fapesp
CNPq
dc.contributor.author.fl_str_mv Germano, Mariana Gomes
Cannavan, Fabiana de Souza
Mendes, Lucas William
Lima, Amanda Barbosa
Teixeira, Wenceslau Geraldes
Pellizari, Vivian Helena
Tsai, Siu Mui
dc.subject.por.fl_str_mv genes catabólicos; diversidade genética; sistemas de uso da terra; ecologia microbiana; solos tropicais
catabolic genes; genetic diversity; land use systems; microbial ecology; tropical soils
topic genes catabólicos; diversidade genética; sistemas de uso da terra; ecologia microbiana; solos tropicais
catabolic genes; genetic diversity; land use systems; microbial ecology; tropical soils
description The objective of this work was to evaluate the catabolic gene diversity for the bacterial degradation of aromatic hydrocarbons in anthropogenic dark earth of Amazonia (ADE) and their biochar (BC). Functional diversity analyses in ADE soils can provide information on how adaptive microorganisms may influence the fertility of soils and what is their involvement in biogeochemical cycles. For this, clone libraries containing the gene encoding for the alpha subunit of aromatic ring‑hydroxylating dioxygenases (α‑ARHD bacterial gene) were constructed, totaling 800 clones. These libraries were prepared from samples of an ADE soil under two different land uses, located at the Caldeirão Experimental Station – secondary forest (SF) and agriculture (AG) –, and the biochar (SF_BC and AG_BC, respectively). Heterogeneity estimates indicated greater diversity in BC libraries; and Venn diagrams showed more unique operational protein clusters (OPC) in the SF_BC library than the ADE soil, which indicates that specific metabolic processes may occur in biochar. Phylogenetic analysis showed unidentified dioxygenases in ADE soils. Libraries containing functional gene encoding for the alpha subunit of the aromatic ring‑hydroxylating dioxygenases (ARHD) gene from biochar show higher diversity indices than those of ADE under secondary forest and agriculture.
publishDate 2012
dc.date.none.fl_str_mv 2012-06-15
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/10028
url https://seer.sct.embrapa.br/index.php/pab/article/view/10028
dc.language.iso.fl_str_mv eng
por
language eng
por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/10028/6912
https://seer.sct.embrapa.br/index.php/pab/article/view/10028/7428
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/10028/5349
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/10028/8045
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.47, n.5, maio 2012: 3rd International Biochar Conference; 654-664
Pesquisa Agropecuária Brasileira; v.47, n.5, maio 2012: 3rd International Biochar Conference; 654-664
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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